269 research outputs found
Gold Nanosystems Covered with Doxorubicin/DNA Complexes: A Therapeutic Target for Prostate and Liver Cancer
Different gold nanosystems covered with DNA and doxorubicin (Doxo) were designed and synthesized for cancer therapy, starting from Au@16-Ph-16 cationic nanoparticles and DNA–Doxo complexes prepared under saturation conditions. For the preparation of stable, biocompatible, and small-sized compacted Au@16-Ph-16/DNA–Doxo nanotransporters, the conditions for the DNA–Doxo compaction process induced by gold nanoparticles were first explored using fluorescence spectroscopy, circular dichroism and atomic force microscopy techniques. The reverse process, which is fundamental for Doxo liberation at the site of action, was found to occur at higher CAu@16-Ph-16 concentrations using these techniques. Zeta potential, dynamic light scattering and UV–visible spectroscopy reveal that the prepared compacted nanosystems are stable, highly charged and of adequate size for the effective delivery of Doxo to the cell. This fact is verified by in vitro biocompatibility and internalization studies using two prostate cancer-derived cell lines (LNCaP and DU145) and one hepatocellular carcinoma-derived cell line (SNU-387), as well as a non-tumor prostate (PNT2) cell line and a non-hepatocarcinoma hepatoblastoma cell line (Hep-G2) model used as a control in liver cells. However, the most outstanding results of this work are derived from the use of the CI+NI combined treatments which present strong action in cancer-derived cell lines, while a protective effect is observed in non-tumor cell lines. Hence, novel therapeutic targets based on gold nanoparticles denote high selectivity compared to conventional treatment based on free Doxo at the same concentration. The results obtained show the viability of both the proposed methodology for internalization of compacted nanocomplexes inside the cell and the effectiveness of the possible treatment and minimization of side effects in prostate and liver cancer
Unveiling the monster heart: unbeamed properties of blazar 4C 71.07
4C 71.07 is a high-redshift blazar whose optical radiation is dominated by
quasar-like nuclear emission. We here present the results of a spectroscopic
monitoring of the source to study its unbeamed properties. We obtained 24
optical spectra at the Nordic Optical Telescope (NOT) and William Herschel
Telescope (WHT) and 3 near-infrared spectra at the Telescopio Nazionale Galileo
(TNG). They show no evidence of narrow emission lines. The estimate of the
systemic redshift from the H and H broad emission lines leads to
. Notwithstanding the nearly face-on orientation
of the accretion disc, the high-ionization emission lines present large
broadening as well as noticeable blueshifts, which increase with the ionizing
energy of the corresponding species. This is a clear indication of strong
ionized outflows. Line broadening and blueshift appear correlated. We applied
scaling relationships to estimate the mass of the supermassive black hole from
the Balmer and C IV lines, taking into account the prescriptions to correct for
outflow. They give . We derived an
Eddington luminosity
, and a broad line region luminosity . The line fluxes do not show significant
variability in time. In particular, there is no line reaction to the jet
flaring activity detected in 2015 October and November. This implies that the
jet gives no contribution to the photoionization of the broad line region in
the considered period.Comment: 13 pages, 13 figures, in press for MNRA
Exploring the circumstellar environment of the young eruptive star V2492 Cyg
Context. V2492 Cyg is a young eruptive star that went into outburst in 2010.
The near-infrared color changes observed since the outburst peak suggest that
the source belongs to a newly defined sub-class of young eruptive stars, where
time-dependent accretion and variable line-of-sight extinction play a combined
role in the flux changes.
Aims. In order to learn about the origin of the light variations and to
explore the circumstellar and interstellar environment of V2492 Cyg, we
monitored the source at ten different wavelengths, between 0.55 \mu m and 2.2
\mu m from the ground and between 3.6 \mu m and 160 \mu m from space.
Methods. We analyze the light curves and study the color-color diagrams via
comparison with the standard reddening path. We examine the structure of the
molecular cloud hosting V2492 Cyg by computing temperature and optical depth
maps from the far-infrared data.
Results. We find that the shapes of the light curves at different wavelengths
are strictly self-similar and that the observed variability is related to a
single physical process, most likely variable extinction. We suggest that the
central source is episodically occulted by a dense dust cloud in the inner
disk, and, based on the invariability of the far-infrared fluxes, we propose
that it is a long-lived rather than a transient structure. In some respects,
V2492 Cyg can be regarded as a young, embedded analog of UX Orionis-type stars.
Conclusions. The example of V2492 Cyg demonstrates that the light variations
of young eruptive stars are not exclusively related to changing accretion. The
variability provided information on an azimuthally asymmetric structural
element in the inner disk. Such an asymmetric density distribution in the
terrestrial zone may also have consequences for the initial conditions of
planet formation.Comment: 9 pages, 7 figures, 2 online tables, accepted for publication in A&
Variability and evolution of the optical polarization of a sample of gamma-ray blazars
We present a polarization variability analysis of a sample of 26 -ray
blazars monitored by the Steward Observatory between 2008 and 2018 in the
optical band. We investigate the properties and long-term variability of their
optical polarization, searching for differences between blazar types. We
observe that BL Lac objects are typically less polarized and less variable than
flat spectrum radio quasars (FSRQs). Moreover, BL Lacs display a distribution
of their polarization angle typically oriented in a preferential direction,
contrary to the rather random distribution of FSRQs. For the latter blazar
type, as well as those sources showing a bright stellar emission, we take into
account the depolarizing effect introduced by the broad line region and the
host galaxy on the measured polarization degree. In this sample we also observe
that BL Lacs present an uncorrelated evolution of the flux and the
polarization. Contrary, FSRQs show a correlation before the depolarization
correction, that is lost however after considering this effect. In addition, we
study the behaviour of the polarization angle, searching for angle rotations in
its long-term evolution. We derive that the FSRQs studied here show rotations
more frequently than BL Lac objects by a factor 1.5. During these periods
we also observe a systematic decrease of the polarization fraction, as well as
a marginal flux increase, not significant however to connect rotations with
optical flares. We interpret these results within the extended shock-in-jet
scenario, able to explain the overall features observed here for the
polarization of the blazar sample.Comment: Accepted for publication in MNRA
Understanding the local and remote source contributions to ambient O3 during a pollution episode using a combination of experimental approaches in the Guadalquivir valley, southern Spain
The Guadalquivir Valley is one of three major O3 hotspots in Spain. An airborne and surface measurement campaign was carried out from July 9th to 11th, 2019 to quantify the local/regional O3 contributions using experimental approaches. Air quality and meteorology data from surface measurements, a microlight aircraft, a helium balloon, and remote sensing data (TROPOMI-NO2-ESA) were used to obtain the 3D distribution of O3 and various tracer pollutants.
O3 accumulation over 2.5 days started with inputs from oceanic air masses transported inland by sea breezes, which drew O3 and its precursors from a local/regional origin to the northeastern end of the basin. The orographic–meteorological setting of the valley caused vertical recirculation of the air masses inside the valley that caused the accumulation by increasing regional background O3 concentration by 25–30 ppb. Furthermore, possible Mediterranean O3 contributions and additional vertical recirculation through the entrainment zone of the convective boundary layer also contributed. Using particulate matter finer than 2.5 μm (PM2.5), ultrafine particles (UFP), and black carbon (BC) as tracers of local sources, we calculated that local contributions increased regional O3 levels by 20 ppb inside specific pollution plumes transported by the breeze into the valley, and by 10 ppb during midday when flying over an area with abundant agricultural burning during the morning. Air masses that crossed the southern boundaries of the Betic system at mid-altitude (400–1850 m a.s.l.) on July 10th and 11th may have provided additional O3. Meanwhile, a decreasing trend at high altitudes (3000–5000 m a.s.l.) was observed, signifying that the impact of stratospheric O3 intrusion decreased during the campaign
Vertical and horizontal distribution of regional new particle formation events in Madrid
The vertical profile of new particle formation (NPF) events was studied by comparing the aerosol size number distributions measured aloft and at surface level in a suburban environment in Madrid, Spain, using airborne instruments. The horizontal distribution and regional impact of the NPF events was investigated with data from three urban, urban background, and suburban stations in the Madrid metropolitan area. Intensive regional NPF episodes followed by particle growth were simultaneously recorded at three stations in and around Madrid during a field campaign in July 2016. The urban stations presented larger formation rates compared to the suburban station. Condensation and coagulation sinks followed a similar evolution at all stations, with higher values at urban stations. However, the total number concentration of particles larger than 2.5 nm was lower at the urban station and peaked around noon, when black carbon (BC) levels are at a minimum. The vertical soundings demonstrated that ultrafine particles (UFPs) are formed exclusively inside the mixed layer. As convection becomes more effective and the mixed layer grows, UFPs are detected at higher levels. The morning soundings revealed the presence of a residual layer in the upper levels in which aged particles (nucleated and grown on previous days) prevail. The particles in this layer also grow in size, with growth rates significantly smaller than those inside the mixed layer. Under conditions with strong enough convection, the soundings revealed homogeneous number size distributions and growth rates at all altitudes, which follow the same evolution at the other stations considered in this study. This indicates that UFPs are detected quasi-homogenously in an area spanning at least 17 km horizontally. The NPF events extend over the full vertical extension of the mixed layer, which can reach as high as 3000 m in the area, according to previous studies. On some days a marked decline in particle size (shrinkage) was observed in the afternoon, associated with a change in air masses. Additionally, a few nocturnal nucleation-mode bursts were observed at the urban stations, for which further research is needed to elucidate their origin.Peer reviewe
Dissecting the long-term emission behaviour of the BL Lac object Mrk 421
We report on long-term multiwavelengthmonitoring of blazar Mrk 421 by the GLAST-AGILE
Support Program of the Whole Earth Blazar Telescope (GASP-WEBT) collaboration and
Steward Observatory, and by the Swift and Fermi satellites. We study the source behaviour in
the period 2007–2015, characterized by several extreme flares. The ratio between the optical,
X-ray and γ -ray fluxes is very variable. The γ -ray flux variations show a fair correlation with
the optical ones starting from 2012.We analyse spectropolarimetric data and find wavelengthdependence
of the polarization degree (P), which is compatible with the presence of the
host galaxy, and no wavelength dependence of the electric vector polarization angle (EVPA).
Optical polarimetry shows a lack of simple correlation between P and flux and wide rotations of
the EVPA.We build broad-band spectral energy distributions with simultaneous near-infrared
and optical data from the GASP-WEBT and ultraviolet and X-ray data from the Swift satellite.
They show strong variability in both flux and X-ray spectral shape and suggest a shift of
the synchrotron peak up to a factor of ∼50 in frequency. The interpretation of the flux and
spectral variability is compatible with jet models including at least two emitting regions that
can change their orientation with respect to the line of sight.http://10.0.4.69/mnras/stx2185Accepted manuscrip
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