3,281 research outputs found
Acidosis Is a Key Regulator of Osteoblast Ecto-Nucleotidase Pyrophosphatase/Phosphodiesterase 1 (NPP1) Expression and Activity
Previous work has shown that acidosis prevents bone nodule formation by osteoblasts in vitro by inhibiting mineralisation of the collagenous matrix. The ratio of phosphate (Pi) to pyrophosphate (PPi) in the bone microenvironment is a fundamental regulator of bone mineralisation. Both Pi and PPi, a potent inhibitor of mineralisation, are generated from extracellular nucleotides by the actions of ecto‐nucleotidases. This study investigated the expression and activity of ecto‐nucleotidases by osteoblasts under normal and acid conditions. We found that osteoblasts express mRNA for a number of ecto‐nucleotidases including NTPdase 1–6 (ecto‐nucleoside triphosphate diphosphohydrolase) and NPP1‐3 (ecto‐nucleotide pyrophosphatase/phosphodiesterase). The rank order of mRNA expression in differentiating rat osteoblasts (day 7) was Enpp1 > NTPdase 4 > NTPdase 6 > NTPdase 5 > alkaline phosphatase > ecto‐5‐nucleotidase > Enpp3 > NTPdase 1 > NTPdase 3 > Enpp2 > NTPdase 2. Acidosis (pH 6.9) upregulated NPP1 mRNA (2.8‐fold) and protein expression at all stages of osteoblast differentiation compared to physiological pH (pH 7.4); expression of other ecto‐nucleotidases was unaffected. Furthermore, total NPP activity was increased up to 53% in osteoblasts cultured in acid conditions (P < 0.001). Release of ATP, one of the key substrates for NPP1, from osteoblasts, was unaffected by acidosis. Further studies showed that mineralised bone formation by osteoblasts cultured from NPP1 knockout mice was increased compared with wildtypes (2.5‐fold, P < 0.001) and was partially resistant to the inhibitory effect of acidosis. These results indicate that increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions
Non-linear response of single-molecule magnets: field-tuned quantum-to-classical crossovers
Quantum nanomagnets can show a field dependence of the relaxation time very
different from their classical counterparts, due to resonant tunneling via
excited states (near the anisotropy barrier top). The relaxation time then
shows minima at the resonant fields H_{n}=n D at which the levels at both sides
of the barrier become degenerate (D is the anisotropy constant). We showed that
in Mn12, near zero field, this yields a contribution to the nonlinear
susceptibility that makes it qualitatively different from the classical curves
[Phys. Rev. B 72, 224433 (2005)]. Here we extend the experimental study to
finite dc fields showing how the bias can trigger the system to display those
quantum nonlinear responses, near the resonant fields, while recovering an
classical-like behaviour for fields between them. The analysis of the
experiments is done with heuristic expressions derived from simple balance
equations and calculations with a Pauli-type quantum master equation.Comment: 4 pages, 3 figures. Submitted to Phys. Rev. B, brief report
Strong Near-Infrared Emission Interior to the Dust-Sublimation Radius of Young Stellar Objects MWC275 and AB Aur
Using the longest optical-interferometeric baselines currently available, we
have detected strong near-infrared (NIR) emission from inside the
dust-destruction radius of Herbig Ae stars MWC275 and AB Aur. Our
sub-milli-arcsecond resolution observations unambiguously place the emission
between the dust-destruction radius and the magnetospheric co-rotation radius.
We argue that this new component corresponds to hot gas inside the
dust-sublimation radius, confirming recent claims based on spectrally-resolved
interferometry and dust evaporation front modeling.Comment: 12 pages, 4 figures, Accepted for publication in ApJ
Extraction of phenolic compounds in controlled macerations of Pedro Ximenez grapes
Crushed grapes of cv. Pedro Ximenez were macerated together with their must for 4, 16, 24 and 48 h at temperatures of 10, 15 and 25°C. The musts obtained after pressing were used for the determination of 14 phenolic compounds from the following fractions: hydroxybenzoic acids, hydroxycinnamic acids, other nonflavonoids, flavan-3-ols and flavonols. The temperature was found not to exert a marked influence on the extraction of the phenolic compounds in the first 16 h of maceration. After that time, however, it had a significant effect, so maceration times longer than 16 h are inadvisable unless subsequent oxidation is applied. The analytical results obtained showed that the flavan-3-ols, catechin and epicatechin were extracted at the highest rates, related with maceration temperature, followed by hydroxbenzoic acids, other nonflavonoids, hydroxycinnamic acids and flavonols. The flavan-3-ol fraction was also extracted in higher proportions under the different conditions assayed, through very closely followed by that of hydroxybenzoic acids
Flavour in "Pedro Ximénez" grape musts subjected to maceration processes
Crushed grapes of Vitis vinifera cv. Pedro Ximenez were macerated at 10, 15 and 25°C for 4, 16, 24 and 48 hours. The musts obtained after pressing were used for the determination of higher alcohols, esters and terpene compounds. The data was subjected to discriminant analysis, obtaining three functions of difficult interpretation. A fruitiness index (SIGMA favourable aromas/SIGMA unfavourable aromas) x 100 and the total of phenolic compounds were subjected to variance and multiple range analyses which revealed significant differences (p<0.01) for maceration times and temperatures. Taking into account the fruitiness index and phenolic compound values, as well as the significant differences obtained by multifactorial (time-temperature) variance analysis, the 24 h-10°C, 4 h-15°C, 16 h-15°C and 24 h-15°C were the best condition
Spatially and Spectrally Resolved Hydrogen Gas within 0.1 AU of T Tauri and Herbig Ae/Be Stars
We present near-infrared observations of T Tauri and Herbig Ae/Be stars with
a spatial resolution of a few milli-arcseconds and a spectral resolution of
~2000. Our observations spatially resolve gas and dust in the inner regions of
protoplanetary disks, and spectrally resolve broad-linewidth emission from the
Brackett gamma transition of hydrogen gas. We use the technique of
spectro-astrometry to determine centroids of different velocity components of
this gaseous emission at a precision orders of magnitude better than the
angular resolution. In all sources, we find the gaseous emission to be more
compact than or distributed on similar spatial scales to the dust emission. We
attempt to fit the data with models including both dust and Brackett
gamma-emitting gas, and we consider both disk and infall/outflow morphologies
for the gaseous matter. In most cases where we can distinguish between these
two models, the data show a preference for infall/outflow models. In all cases,
our data appear consistent with the presence of some gas at stellocentric radii
of ~0.01 AU. Our findings support the hypothesis that Brackett gamma emission
generally traces magnetospherically driven accretion and/or outflows in young
star/disk systems.Comment: 48 pages, including 17 figures. Accepted for publication by Ap
Neural network monitoring of resistance welding processes
Control of weld quality is one of the most important and complex processes to be carried out on production lines. Neural networks have shown good results in fields such as modelling and control of physical processes. It is suggested in this article that a neural classifier should be used to carry out non‐destructive on‐line analysis. This system has been developed and installed at resistance welding stations. Results confirm the validity of neural networks used for this type of application
The effect of prolonged simulated non- gravitational environment on mineral balance in the adult male, volume 1 Final report
Effect of prolonged bed rest with simulated weightlessness on mineral balance in male adult - Vol.
Science with the Keck Interferometer ASTRA Program
The ASTrometric and phase-Referenced Astronomy (ASTRA) project will provide
phase referencing and astrometric observations at the Keck Interferometer,
leading to enhanced sensitivity and the ability to monitor orbits at an
accuracy level of 30-100 microarcseconds. Here we discuss recent scientific
results from ASTRA, and describe new scientific programs that will begin in
2010-2011. We begin with results from the "self phase referencing" (SPR) mode
of ASTRA, which uses continuum light to correct atmospheric phase variations
and produce a phase-stabilized channel for spectroscopy. We have observed a
number of protoplanetary disks using SPR and a grism providing a spectral
dispersion of ~2000. In our data we spatially resolve emission from dust as
well as gas. Hydrogen line emission is spectrally resolved, allowing
differential phase measurements across the emission line that constrain the
relative centroids of different velocity components at the 10 microarcsecond
level. In the upcoming year, we will begin dual-field phase referencing (DFPR)
measurements of the Galactic Center and a number of exoplanet systems. These
observations will, in part, serve as precursors to astrometric monitoring of
stellar orbits in the Galactic Center and stellar wobbles of exoplanet host
stars. We describe the design of several scientific investigations capitalizing
on the upcoming phase-referencing and astrometric capabilities of ASTRA.Comment: Published in the proceedings of the SPIE 2010 conference on "Optical
and Infrared Interferometry II
Radial Structure in the TW Hya Circumstellar Disk
We present new near-infrared interferometric data from the CHARA array and the Keck Interferometer on the circumstellar disk of the young star, TW Hya, a proposed "transition disk." We use these data, as well as previously published, spatially resolved data at 10 μm and 7 mm, to constrain disk models based on a standard flared disk structure. We find that we can match the interferometry data sets and the overall spectral energy distribution with a three-component model, which combines elements at spatial scales proposed by previous studies: optically thin, emission nearest the star, an inner optically thick ring of emission at roughly 0.5 AU followed by an opacity gap and, finally, an outer optically thick disk starting at ~4 AU. The model demonstrates that the constraints imposed by the spatially resolved data can be met with a physically plausible disk but this requires a disk containing not only an inner gap in the optically thick disk as previously suggested, but also a gap between the inner and outer optically thick disks. Our model is consistent with the suggestion by Calvet et al. of a planet with an orbital radius of a few AU. We discuss the implications of an opacity gap within the optically thick disk
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