Changes in the secretory profile of NSCLC-associated fibroblasts after ablative radiotherapy: potential impact on angiogenesis and tumor growth

In the context of radiotherapy, collateral effects of ablative ionizing radiation (AIR) on stromal components of tumors remains understudied. In this work, cancer-associated fibroblasts (CAFs) isolated from freshly resected human lung tumors were exposed to AIR (1x18Gy) and analyzed for their release of paracrine factors. Inflammatory mediators and regulators of angiogenesis and tumor growth were analyzed by multiplex protein assays in conditioned medium (CM) from irradiated and non-irradiated CAFs. Additionally, the profile of secreted proteins was examined by proteomics. In functional assays, effects of CAF-CM on proliferative and migratory capacity of lung tumor cells (H-520/H-522) and endothelial cells (HUVECs), and on the tube-forming capacity of endothelial cells was assessed. Our data show that exposure of CAFs to ablative doses of ionizing radiation results in a) down-regulated release of angiogenic factors SDF-1, angiopoietin and thrombospondin-2; b) up-regulated release of growth factor bFGF from most donors, and c) unaffected expression-levels of HGF and inflammatory mediators IL-6, IL-8, IL-1ƒÒ and TNF-£. Conditioned medium from irradiated and control CAFs did not affect differently the proliferative or migratory capacity of tumor cells (H-520/H-522), whereas migratory capacity of endothelial HUVEC cells was partially reduced in the presence of irradiated CAF conditioned medium. Overall we conclude that AIR mediates a transformation on the secretory profile of CAFs that could influence the behavior of other cells in the tumor tissue and hence guide to some extent therapeutic outcomes. The downstream consequences of the changes observed in this study merits further investigations

Logarithmic behavior of degradation dynamics in metal--oxide semiconductor devices

In this paper the authors describe a theoretical simple statistical modelling of relaxation process in metal-oxide semiconductor devices that governs its degradation. Basically, starting from an initial state where a given number of traps are occupied, the dynamics of the relaxation process is measured calculating the density of occupied traps and its fluctuations (second moment) as function of time. Our theoretical results show a universal logarithmic law for the density of occupied traps $\bar{} \sim \phi (T,E_{F}) (A+B \ln t)$, i.e., the degradation is logarithmic and its amplitude depends on the temperature and Fermi Level of device. Our approach reduces the work to the averages determined by simple binomial sums that are corroborated by our Monte Carlo simulations and by experimental results from literature, which bear in mind enlightening elucidations about the physics of degradation of semiconductor devices of our modern life

Seeking the Ultraviolet Ionizing Background at z~3 with the Keck Telescope

We describe the initial results of a deep long-slit emission line search for redshifted (2.7<z<4.1) Lyman-alpha. These observations are used to constrain the fluorescent Ly-alpha emission from the population of clouds whose absorption produces the higher-column-density component of the Ly-alpha forest in quasar spectra. We use the results to set an upper limit on the ultraviolet ionizing background. Our spectroscopic data obtained with the Keck II telescope at lambda/(Delta lambda FWHM)~2000 reveals no candidate Ly-alpha emission over the wavelength range of 4500-6200 Ang along a 3 arcmin slit in a 5400 s integration. Our 3 sigma upper bound on the mean intensity of the ionizing background at the Lyman limit is J(nu 0) < 2E-21 erg/s/cm**2/Hz/sr for 2.7<z<3.1 (where we are most sensitive), assuming Lyman limit systems have typical radii of 70 kpc (q_0=0.5, H_0=50 km/s/Mpc). This constraint is more than an order of magnitude more stringent than any previously published direct limit. However, it is still a factor of three above the ultraviolet background level expected due to the integrated light of known quasars at z~3. This pilot study confirms the conclusion of Gould \& Weinberg (1996) that integrations of several hours on a 10-m class telescope should be capable of measuring J(nu 0) at high redshift.Comment: 22 pages, 2 postscipt figures. Latex requires aaspp4.sty and epsf.sty (included). Accepted for publication in the Astronomical Journal (Nov 1998

Synthetic and structural studies of mixed sodium bis(trimethylsilyl)amide/sodium halide aggregates in the presence of η2-N,N-, η3-N,N,N/N,O,N-, and η4-N,N,N,N-donor ligands

When the important utility amide sodium bis(trimethylsilyl)amide is combined with hydrocarbon-soluble molecular forms of sodium halide salts, a complex structural chemistry is observed. Through utilization of several different multidentate donor molecules, it is possible to isolate a series of compounds that have structural motifs previously not observed in sodium chemistry

Log canonical thresholds of Del Pezzo Surfaces in characteristic p

The global log canonical threshold of each non-singular complex del Pezzo surface was computed by Cheltsov. The proof used Koll\'ar-Shokurov's connectedness principle and other results relying on vanishing theorems of Kodaira type, not known to be true in finite characteristic. We compute the global log canonical threshold of non-singular del Pezzo surfaces over an algebraically closed field. We give algebraic proofs of results previously known only in characteristic $0$. Instead of using of the connectedness principle we introduce a new technique based on a classification of curves of low degree. As an application we conclude that non-singular del Pezzo surfaces in finite characteristic of degree lower or equal than $4$ are K-semistable.Comment: 21 pages. Thorough rewrite following referee's suggestions. To be published in Manuscripta Mathematic

Analogue mouse pointer control via an online steady state visual evoked potential (SSVEP) brain-computer interface

The steady state visual evoked protocol has recently become a popular paradigm in brain–computer interface (BCI) applications. Typically (regardless of function) these applications offer the user a binary selection of targets that perform correspondingly discrete actions. Such discrete control systems are appropriate for applications that are inherently isolated in nature, such as selecting numbers from a keypad to be dialled or letters from an alphabet to be spelled. However motivation exists for users to employ proportional control methods in intrinsically analogue tasks such as the movement of a mouse pointer. This paper introduces an online BCI in which control of a mouse pointer is directly proportional to a user's intent. Performance is measured over a series of pointer movement tasks and compared to the traditional discrete output approach. Analogue control allowed subjects to move the pointer faster to the cued target location compared to discrete output but suffers more undesired movements overall. Best performance is achieved when combining the threshold to movement of traditional discrete techniques with the range of movement offered by proportional control

The ultracool dwarf DENIS-P J104814.7-395606. Chromospheres and coronae at the low-mass end of the main-sequence

We have obtained an XMM-Newton observation and a broad-band spectrum from the ultraviolet to the near infrared with X-Shooter for one of the nearest M9 dwarfs, DENIS-P J1048-3956 (4pc). We integrate these data by a compilation of activity parameters for ultracool dwarfs from the literature with the aim to advance our understanding of these objects by comparing them to early-M type dwarf stars and the Sun. Our deep XMM-Newton observation has led to the first X-ray detection of DENIS-P J1048-3956 (log Lx = 25.1) as well as the first measurement of its V band brightness (V = 17.35mag). Flux-flux relations between X-ray and chromospheric activity indicators are here for the first time extended into the regime of the ultracool dwarfs. The approximate agreement of DENIS-P J1048-3956 and other ultracool dwarfs with flux-flux relations for early-M dwarfs suggests that the same heating mechanisms work in the atmospheres of ultracool dwarfs, albeit weaker as judged from their lower fluxes. The observed Balmer decrements of DENIS-P J1048-3956 are compatible with optically thick plasma in LTE at low, nearly photospheric temperature or optically thin LTE plasma at 20000K. Describing the decrements with CaseB recombination requires different emitting regions for Halpha and the higher Balmer lines. The high observed Halpha/Hbeta flux ratio is also poorly fitted by the optically thin models. We derive a similarly high value for the Halpha/Hbeta ratio of vB10 and LHS2065 and conclude that this may be a characteristic of ultracool dwarfs. We add DENIS-P J1048-3956 to the list of ultracool dwarfs detected in both the radio and the X-ray band. The Benz-Guedel relation between radio and X-ray luminosity of late-type stars is well-known to be violated by ultracool dwarfs. We speculate on the presence of two types of ultracool dwarfs with distinct radio and X-ray behavior.Comment: accepted for publication in Astronomy & Astrophysic

The first Frontier Fields cluster: 4.5{\mu}m excess in a z~8 galaxy candidate in Abell 2744

We present in this letter the first analysis of a z~8 galaxy candidate found in the Hubble and Spitzer imaging data of Abell 2744, as part of the Hubble Frontier Fields legacy program. We applied the most commonly-used methods to select exceptionally high-z galaxies by combining non-detection and color-criteria using seven HST bands. We used GALFIT on IRAC images for fitting and subtracting contamination of bright nearby sources. The physical properties have been inferred from SED-fitting using templates with and without nebular emission. This letter is focussed on the brightest candidate we found (m$_{F160W}$=26.2) over the 4.9 arcmin$^2$ field of view covered by the WFC3. It shows a non-detection in the ACS bands and at 3.6{\mu}m whereas it is clearly detected at 4.5{\mu}m with rather similar depths. This break in the IRAC data could be explained by strong [OIII]+H{\beta} lines at z~8 which contribute to the 4.5{\mu}m photometry. The best photo-z is found at z~8.0$^{+0.2}_{-0.5}$, although solutions at low-redshift (z~1.9) cannot be completely excluded, but they are strongly disfavoured by the SED-fitting work. The amplification factor is relatively small at {\mu}=1.49$\pm$0.02. The Star Formation Rate in this object is ranging from 8 to 60 Mo/yr, the stellar mass is in the order of M$_{\star}$=(2.5-10) x 10$^{9}$Mo and the size is r~0.35$\pm$0.15 kpc. This object is one of the first z~8 LBG candidates showing a clear break between 3.6{\mu}m and 4.5{\mu}m which is consistent with the IRAC properties of the first spectroscopically confirmed galaxy at a similar redshift. Due to its brightness, the redshift of this object could potentially be confirmed by near infrared spectroscopy with current 8-10m telescopes. The nature of this candidate will be revealed in the coming months with the arrival of new ACS and Spitzer data, increasing the depth at optical and near-IR wavelengths.Comment: 4 pages, 2 figures, Accepted for publication in Astronomy and Astrophysics Letter