Proteomic analysis of Arabidopsis protein S-nitrosylation in response to inoculation with <em>Pseudomonas syringae</em>.

Nitric oxide (NO) is a key signaling molecule in plants, being its biological effects mainly mediated through S-nitrosylation of cysteine thiols. Using the biotin switch method combined with mass spectrometry analysis we have identified 127 targets of S-nitrosylation in Arabidopsis cell suspension cultures and leaves challenged with virulent and avirulent isolates of Pseudomonas syringae pv. tomato. The NO targets are proteins associated with carbon, nitrogen, and sulpfur metabolism, photosynthesis, the cytoskeleton, stress-, pathogen- and redox-related and signaling proteins. Some proteins were previously identified in plants and mammals, while others (63%) represent novel targets of S-nitrosylation. Our data suggest that NO might be orchestrating the whole plant physiology, presumably through covalent modification of proteins

Fourteen years of plant proteomics reflected in Proteomics: Moving from model species and 2DE-based approaches to orphan species and gel-free platforms.

In this article, the topic of plant proteomics is reviewed based on related papers published in the journal Proteomics since publication of the first issue in 2001. In total, around 300 original papers and 41 reviews published in Proteomics between 2000 and 2014 have been surveyed. Our main objective for this review is to help bridge the gap between plant biologists and proteomics technologists, two often very separate groups. Over the past years a number of reviews on plant proteomics have been published [1-7]. To avoid repetition we have focused on more recent literature published after 2010, and have chosen to rather make continuous reference to older publications. The use of the latest proteomics techniques and their integration with other approaches in the "systems biology" direction are discussed more in detail. Finally we comment on the recent history, state of the art, and future directions of plant proteomics, using publications in Proteomics to illustrate the progress in the field. The review is organized into two major blocks, the first devoted to provide an overview of experimental systems (plants, plant organs, biological processes) and the second one to the methodology

Influencia de la fecha de plantación al establecimiento de "Pinus halepensis" Mill.: aplicación de la proteómica a estudios de ecofisiología en campo

Pinus halepensis Mill. en el ámbito mediterráneo, y en particular en ambientes semiáridos, ha demostrado ser una especie con buena adaptabilidad y capaz de sobrevivir en situaciones de fuerte estrés hídrico siendo una de las especies más utilizadas en los programas de reforestación del sureste español. La proteómica tiene como objetivo el estudio del proteoma de los diferentes seres vivos, es decir, el estudio de las proteínas presentes en una unidad biológica, en un momento concreto y bajo condiciones ambientales determinadas, siendo un buen indicador del estado fisiológico de la planta y su respuesta a estrés. El objetivo de este trabajo es aplicar la proteómica como herramienta para el estudio del efecto de la fecha de plantación sobre una repoblación de Pinus halepensis en el levante español bajo condiciones de estrés. La fecha de plantación tuvo efecto sobre la variable crecimiento en términos de altura, diámetro y esbeltez y sobre el potencial hídrico, no siendo demasiado notable su influencia sobre la supervivencia o la fluorescencia de la clorofila. Mediante un análisis de proteómica previo, se encontraron algunas diferencias a nivel de expresión de proteínas que podrían servir como marcadores moleculares de estrés hídrico una vez hayan sido identificadas

Facing challenges in Proteomics today and in the coming decade: Report of Roundtable Discussions at the 4th EuPA Scientific Meeting, Portugal, Estoril 2010

Roundtable Discussion sessions were conceived for the first time at the 4th Edition of the EuPA Annual Scientific Meeting that took place in Estoril, Portugal, from the 23rd to 27th of October, 2010. Under the theme “2010: a proteomics odyssey towards the next decades”, young and senior scientists had the opportunity to discuss particular issues of different proteomic areas s distributed by eleven luncheon roundtables moderated by recognized experts in the field. During two hours, the roundtable discussions proceeded in a friendly environment, in which the participants (about 8–10 per roundtable) were encouraged to ask questions and express their opinions without restraint (Fig. 1). The topics covered a range of recurring issues such as the capabilities and limitations of several proteomic methodologies and suggestions on how researchers may better overcome them. The major goal devised by roundtable leaders was to provide independent advice, describe limitations, give practical tips, help set realistic expectations and answer questions from the participants. Participants were also encouraged to be ambitious in generating new solutions or strategies for the future

From 2003 to 2011: Proteomics investigation at the agroforestry and plant biochemistry and proteomics research group (University of Cordoba, Spain)

ISBN: 9784904633991International audienc