Structural characterization of functionalized gold nanoparticles for drug delivery in cancer therapy: a NMR based approach

In the present paper, we report results from a study of the structure and physicochemical properties of gold nanoparticles modified with poly(ethylene glycol) (PEG) designed for the drug delivery of the proteasome inhibitor Bortezomib (BTZ) in cancer therapy. A number of advanced analytical techniques were used to define important physicochemical characteristics such as composition, structure, surface properties, particle size and morphology. A new approach based on detailed NMR studies was employed to define specific intermolecular interactions and mechanisms of drug immobilization and location into surface modified gold nanoparticles (AuNPs). Particularly important information was gained from analysis of NMR spectroscopic parameters such as the spectral line shape, translation diffusion, the nuclear Overhauser effect (NOE) and spin-lattice relaxation (T-1). The results confirmed the coexistence of two different types of BTZ inclusion into polyethylene glycol coated gold nanoparticles: (i) association with the polymer chains by weak H-bonds and/or dipole-charge interactions and (ii) adsorption on the surface of the gold nanoparticles. The results allowed for determination of the overall structure of Bortezomib loaded PEG coated AuNPs, which is related to the therapeutic drug efficacy and activity in the treatment of cancer

Redox Proteomic Analysis Reveals Oxidative Modifications of Proteins by Increased Levels of Intracellular Reactive Oxygen Species During Hypoxia Adaptation of Aspergillus fumigatus

We thank Silke Steinbach, Till Kindel, and Michael Cyrulies for their excellent technical assistance. Work of T.K., O.K. and A.A.B was supported by the Deutsche Forschungsge-meinschaft within the Collaborative Research Center TR124 FungiNet (project A1 and Z2).The work of E.S. was supported by the International Leibniz Research School for Microbial and Biomolecular Interactions (ILRS)and by the Medical Research Council Centre for Medical Mycology at the University of Aberdeen (MR/N006364/1).We thank Matthew Blango and Falk Hillmann for the critical reading of the manuscript.Peer reviewedPostprin

The teacher’s self-presentation in terms of students’ assessments or the study of the teacher’s self-presentation features

The article is devoted to the study of the phenomenon of self-presentation, as a significant mechanism that helps to build effective communication between teachers and students. To study the described phenomenon, we used the author's questionnaire "Assessment of the image of the teacher." A study conducted on a sample of teachers and their students led us to the conclusion that there are differences between the “I-image” of teachers and the “I-expert”, i.e. between teachers' self-image and student grading system. Teachers evaluate themselves higher in terms of the studied parameters than their students evaluate. As a result, we came to the empirical conclusion that it is important for teachers to pay attention to the development of self-presentation skills

The teacher’s self-presentation in terms of students’ assessments or the study of the teacher’s self-presentation features

The article is devoted to the study of the phenomenon of self-presentation, as a significant mechanism that helps to build effective communication between teachers and students. To study the described phenomenon, we used the author's questionnaire "Assessment of the image of the teacher." A study conducted on a sample of teachers and their students led us to the conclusion that there are differences between the “I-image” of teachers and the “I-expert”, i.e. between teachers' self-image and student grading system. Teachers evaluate themselves higher in terms of the studied parameters than their students evaluate. As a result, we came to the empirical conclusion that it is important for teachers to pay attention to the development of self-presentation skills

Transformation of the concept of "power" as a basic cultural value in the media discourse of modern times in metagraphic representation

The relevance is due to the fact that new geopolitical realities cause the transformation of basic concepts, which include the concept of "power", which has an internal conflict of content, where there is power as self-possession and power as the management of others. At the same time, the concept of "power" itself is built in various linguistic and philosophical coordinate systems. The research is based on an ethnic-psycholinguistic scientific paradigm, within which the concept of "power" is analyzed from the point of view of structure and semantics as a separate element of linguistic consciousness, actively represented in the speech activity of representatives of various linguistic cultures (Kazakh, Russian, Anglo-American, Chinese). The appeal to this concept is predetermined by the acuteness of the experience of the problem of power itself as a constantly evolving socio-political institution. The aim of the study is to model the concept of "power" in the modern linguistic consciousness of modern times with the establishment of its structural and content specificity. The complexity of the claimed topic is predetermined by the initial ambiguity of power as a social phenomenon, which includes a binary of oppositions: "I" individual and "We" collective, the ability to control oneself and the ability to control others, legitimate power and illegitimate power, ethical power due to respect and authority and the power of oppression and pressure due to fear. The metagraph of the complex socio-political basic value "power" perfectly illustrates the representation of this complex concept, demonstrating qualitative transformations in the structure of the meaning of this concept at different levels of lexico-semantic, neurasthenic and symbolic-semiological hierarchy

The structure and properties of boron carbide ceramics modified by high-current pulsed electron-beam

The present work is devoted to numerical simulation of temperature fields and the analysis of structural and strength properties of the samples surface layer of boron carbide ceramics treated by the high-current pulsed electron-beam of the submillisecond duration. The samples made of sintered boron carbide ceramics are used in these investigations. The problem of calculating the temperature field is reduced to solving the thermal conductivity equation. The electron beam density ranges between 8…30 J/cm2, while the pulse durations are 100…200 μs in numerical modelling. The results of modelling the temperature field allowed ascertaining the threshold parameters of the electron beam, such as energy density and pulse duration. The electron beam irradiation is accompanied by the structural modification of the surface layer of boron carbide ceramics either in the single-phase (liquid or solid) or two-phase (solid-liquid) states. The sample surface of boron carbide ceramics is treated under the two-phase state (solid-liquid) conditions of the structural modification. The surface layer is modified by the high-current pulsed electron-beam produced by SOLO installation at the Institute of High Current Electronics of the Siberian Branch of the Russian Academy of Sciences, Tomsk, Russia. The elemental composition and the defect structure of the modified surface layer are analyzed by the optical instrument, scanning electron and transmission electron microscopes. Mechanical properties of the modified layer are determined measuring its hardness and crack resistance. Research results show that the melting and subsequent rapid solidification of the surface layer lead to such phenomena as fragmentation due to a crack network, grain size reduction, formation of the sub-grained structure due to mechanical twinning, and increase of hardness and crack resistance

Oxidative stress, a trigger of hepatitis C and B virus-induced liver carcinogenesis

Virally induced liver cancer usually evolves over long periods of time in the context of a strongly oxidative microenvironment, characterized by chronic liver inflammation and regeneration processes. They ultimately lead to oncogenic mutations in many cellular signaling cascades that drive cell growth and proliferation. Oxidative stress, induced by hepatitis viruses, therefore is one of the factors that drives the neoplastic transformation process in the liver. This review summarizes current knowledge on oxidative stress and oxidative stress responses induced by human hepatitis B and C viruses. It focuses on the molecular mechanisms by which these viruses activate cellular enzymes/systems that generate or scavenge reactive oxygen species (ROS) and control cellular redox homeostasis. The impact of an altered cellular redox homeostasis on the initiation and establishment of chronic viral infection, as well as on the course and outcome of liver fibrosis and hepatocarcinogenesis will be discussed The review neither discusses reactive nitrogen species, although their metabolism is interferes with that of ROS, nor antioxidants as potential therapeutic remedies against viral infections, both subjects meriting an independent review.publishersversionPeer reviewe

Cyrillic Manuscript Heritage: Vol. 37

CMH 37 cover design based on the cover design of CMH 34 created by Eva Dujardin Dale.Note that in 2015, only one issue appeared of the biannual newsletter appeared: Cyrillic Manuscript Heritage 37 (December 2015). This issue contains information and photos of the Eighth Biennial Medieval Slavic Summer Institute, which was held during the summer of 2015.Biannual newsletter of the Hilandar Research Library (HRL) and the Resource Center for Medieval Slavic Studies (RCMSS), The Ohio State University. Includes: features by guest contributors, which appear under the column heading of "HRL Journal" – where Cyrillic Manuscript Heritage asks researchers who have used the resources of the HRL in the past year or so to describe their experience and work; a column, "Director's Desk," by the Director of RCMSS; Reports on recent visitors, research, events, exhibits, projects, updates, etc.; Contributors to both the Hilandar Endowment Fund and Hilandar's Friends of the Library Fund are listed, as are donors of "Gifts in Kind." Also included are seasonal announcements regarding the International Hilandar Conference series and the Medieval Slavic Summer Institute.Front cover image: Hodigetria Mosaic icon, 12th century, which Stefan Nemanja (canonized as St. Simeon) is said to have brought to Mount Athos after he took monastic vows in 1198. Image is from a set of slides donated to the HRL by A. Dean McKenzie, professor emeritus of Art History at the University of Oregon; table of contents, front cover; masthead and description of front cover image, p. 2; "From the Director's Desk," by Dr. Predrag Matejic, photos by M.A. Johnson and Jessi Jones, pp. 2-3, 5; "The 2015 Kalamazoo Conference," photos by M.A. Johnson, p. 4; "Welcome Ryan Perkins!," photo courtesy of Opic [the OSU Avatar Service], p. 4; "New Leadership at OSU Libraries," photos courtesy of OSU Libraries, p. 6; "Exploring the 'World's Desire,'" by Kevin Bloomfield, photos by Jessi Jones, p. 6; "Kopitar's Legacy of Slavic Manuscripts in Slovenia and Austria," by M.A. Johnson, photos by M.A. Johnson, p. 7; "HRL Journal: Traversing the Annals of Time," by Ezekiel Abodale Olagoke, photo by Jessi Jones, p. 8; "Getting 'Hands On' at Hilandar Research Library," by Tom Elvins, photos by Jessi Jones, p. 9; "MSSI 2015," photos by Nina Haviernikova, M.A. Johnson, and Jessi Jones, pp. 10-11; "Examining Exegesis and Hermeneutics at HRL," by Maria Ivanova, photo by Jessie Jones, p. 12; "Letter from Dr. Vlada Stanković," p. 12; "2015 Highlights," photos by Nina Haviernikova and courtesy of St. Vladimir's Orthodox Theological Seminary (SVOTS), p. 13; "HRL Acquires the Emilia Guergova-Veder & William R. Veder Collection," p. 13; "Edward Kasinec Request," photo courtesy of Edward Kasinec, p. 14; "Special Collections Donor Wall," photo by Jessi Jones, p. 14; Contributions to the Hilandar Endowment Fund, Gifts through Friends of the Library, Gifts in Kind to the Hilandar Research Library, p. 15; farewell to OSUL colleague Wes Boomgaarden (retired), photo by Eric Albrecht, and "Happy Holidays and Season's Greetings," back cover