Ways of optimizing medical services for children at educational institutions under the Health Care system reform in Ukraine.

Aim – scientific substantiation of the ways to optimize preventive childhood medicine under the health care system reform in Ukraine. The medical, sanitary and epidemiological conditions of children’s stay were studied at 147 schools in five oblasts of Ukraine and the city of Kyiv. The following methods were used: system analysis, analytical, mathematical and statistical, bibliosemantic, modeling and expert assessments. The results of an expert assessment (224 specialists) on the optimization of medical services for schoolchildren were analyzed. The main risk factors for schoolchildren staying at educational institutions were identified: daily routine failure, non-compliance with the hygiene requirements for the premises where the educational process is carried out, as well as failure in food and water quality and safety. The levels of medical observation provision were analyzed. There were suggested two ways of maintaining the role of medical personnel in preserving and enhancing the health of schoolchildren: involving doctors of primary care centers of the Ministry of Health of Ukraine and creating an autonomous medical service in the institutions of the Ministry of Education and Science of Ukraine in accordance with the current licensing requirements. The issues of prevention and promotion of children's health, including schoolchildren, are one of the key aspects of the training of medical personnel at all stages. Such programs need to be modernized on the basis of a competency approach, taking into account the current needs of the health care system and the best international experience in resolving these issues

МЕТОДИ КЛАСТЕРИЗАЦІЇ В ПРОГРАМІ MICROARRAYTOOL ДЛЯ АНАЛІЗУ ДАНИХ ДНК-МІКРОАРРЕЇВ

Microarray technologies (DNA chips) allow to perform a quantitative analysis of expression of ten thousands genes. In this work a novel Microarraytool program was developed which allows to perform the cluster analysis and to compare the different experiments data by statistical analysis. Several clustering algorithms have been implemented into Microarraytool program: hierarchical clustering, k-means clustering, self-organizing maps (SOM) algorithm and self-organizing tree maps (SOTA) algorithm. The testing of these algorithms was performed using the Stanford Microarray Database for expression of 8613 individual genes in human fibroblasts after stimulation. The testing procedure revealed a correct performance of these algorithms implemented into Microarraytool program.Микроаррей-технологии или ДНК-чипы позволяют проводить количественный анализ экспрессии десятков тысяч генов. В данной работе описана новая программа Microarraytool для анализа ДНК микроаррей-данных, которая позволяет проводить трансформацию и нормализацию данных, выполнять кластерный анализ и сравнивать разные эксперименты с помощью статистического анализа. В программе имплементированы такие методы кластерного анализа: иерархический кластерний анализ, метод кластеризации k-средних, карты самоорганизующихся признаков (SOM) и SOTA- кластеризация. Проведено тестирование алгоритмов кластерного анализа для микроаррей-данных Стэнфордской базы данных по экспрессии первичных фибробластов человека для 8613 индивидуальных генов на разных временных промежутках после стимуляции. Анализ данных показал корректное выполнение алгоритмов, имплементированных в программе Microarraytool.Мікроаррей-технології або ДНК-чіпи дозволяють проводити кількісний аналіз експресії десятків тисяч генів. В даній роботі описана нова програма Microarraytool для аналізу ДНК мікроаррей-даних, яка дозволяє проводити трансформацію та нормалізацію даних, виконувати кластерний аналіз та порівнювати різні експерименти за допомогою статистичного аналізу. Імплементовано такі методи кластерного аналізу: ієрархічний кластерний аналіз, метод кластеризації k-середніх, карти ознак, що самоорганізуються (SOM) та SOTA-кластеризація. Проведено тестування алгоритмів для кластерного аналізу для мікроаррей-даних Стенфордської бази даних з експресії первинних фібробластів людини для 8613 індивідуальних генів на різних часових проміжках після стимуляції. Аналіз даних показав коректне виконання алгоритмів, імплементованих в програмі Microarraytool

Conflict resolution

U radu se na sustavan i jezgrovit način objašnjavaju temeljne značajke sukoba, vrste sukoba te način podučavanja djece konstruktivnom, nenasilnom rješavanju istih. Istraživanje započinje definiranjem osnovnih značajki komunikacije i naglašavanjem važnosti same sposobnosti komuniciranja. Čovjek je od najranije dobi u svakodnevnoj komunikaciji s ljudima oko sebe. Ona je temelj za razvoj svih daljnjih socijalnih vještina. Socijalna kompetencija razvija se tijekom odrastanja prvo u roditeljskom domu, a zatim i kroz socijalnu interakciju s drugim ljudima. Sposobnost socijalne interakcije nužno je razvijati te je bitno kod djece poticati razvoj socijalnih vještina, jer nam one omogućavaju da u komunikaciji s okolinom ostvarimo sve svoje želje i potrebe. Socijalne vještine koje kod djece treba posebno poticati su vještine slušanja, suradnje i empatije te ujedno i samopoštovanje. Djeca moraju naučiti na koji način reagirati na sukob i to će uvelike utjecati na kvalitetu njihova života izvan i unutar zajednice. Teorijske spoznaje o komunikaciji, stjecanju socijalnih vještina, te učenju konstruktivnog rješavanja sukoba upotpunjene su praktičnim saznanjima te provođenjem radionica konstruktivnog rješavanja sukoba s djecom predškolske dobi.This thesis systematically and concisely explains fundamental principles of conflict, types of conflict and ways of teaching children about constructive, nonviolent resolution of such. Research begins by defining basic principles of communication and emphasizing importance of the sole ability to communicate. From the earliest age people are daily involved in communication with others. This is the foundation for development of any other social skill. Social competence is being developed during growing up, it begins in the family home and it continues through social interaction with other people. It is essential to develop social interaction skills and it is also important to encourage development of these skills in children, because social skills enable us to achieve all our desires and needs through communication. Social skills that should be specially encouraged in children are skills of listening, cooperation, empathy towards others and self respect. . Children must learn how to react to conflict and this will greatly influence the quality of their personal life as well as their life within the community. Theoretical knowledge on communication, on growing social skills and on learning how to constructively resolve conflicts is accompanied with practical findings and through workshops on constructive conflict resolution among children of preschool age

The first experience of application of robotic systems for stereotactic hypofractionated radiotherapy "CyberKnife" in the treatment of patients with renal cell cancer

Presents the first experience of application of stereotactic RA-diotherapy on the robotic system 'CyberKnife* in the establishment of health care Chelyabinsk Regional Clinical Oncological Dispensary in patients with renal cell cancer. Treatment received 2 patients with renal cell carcinoma, and one patient carried out the irradiation of both kidneys. As a result, the first patient received a com-plete tumor resorption of the right kidney and reducing the size and lowering the contrast of the tumor of the left kidney. Kidney function is not deteriorated. The second patient has decreased the tumor size, but retains the contrast in the vascular phase. The function of a single kidney is not deteriorated.Представлен первый опыт применения стереотаксической радиотерапии на роботизированной системе «CyberKnife» в ГБУЗ «Челябинский Областной Клинический Онкологический Диспансер» у больных почечно-клеточным раком. Лечение получили 2 пациента с почечно-клеточным раком, причем у одного пациента проведено облучение обеих почек. В результате у первого пациента получена полная резорбция опухоли правой почки и уменьшение размеров и снижение контрастирования опухоли левой почки. Функция почек не ухудшилась. У второго пациента уменьшились размеры опухоли, но сохраняется контрастирование в сосудистые фазы. Функция единственной почки не ухудшилась

Charting the NF-κB Pathway Interactome Map

Inflammation is part of a complex physiological response to harmful stimuli and pathogenic stress. The five components of the Nuclear Factor κB (NF-κB) family are prominent mediators of inflammation, acting as key transcriptional regulators of hundreds of genes. Several signaling pathways activated by diverse stimuli converge on NF-κB activation, resulting in a regulatory system characterized by high complexity. It is increasingly recognized that the number of components that impinges upon phenotypic outcomes of signal transduction pathways may be higher than those taken into consideration from canonical pathway representations. Scope of the present analysis is to provide a wider, systemic picture of the NF-κB signaling system. Data from different sources such as literature, functional enrichment web resources, protein-protein interaction and pathway databases have been gathered, curated, integrated and analyzed in order to reconstruct a single, comprehensive picture of the proteins that interact with, and participate to the NF-κB activation system. Such a reconstruction shows that the NF-κB interactome is substantially different in quantity and quality of components with respect to canonical representations. The analysis highlights that several neglected but topologically central proteins may play a role in the activation of NF-κB mediated responses. Moreover the interactome structure fits with the characteristics of a bow tie architecture. This interactome is intended as an open network resource available for further development, refinement and analysis

Parsimonious Higher-Order Hidden Markov Models for Improved Array-CGH Analysis with Applications to Arabidopsis thaliana

Array-based comparative genomic hybridization (Array-CGH) is an important technology in molecular biology for the detection of DNA copy number polymorphisms between closely related genomes. Hidden Markov Models (HMMs) are popular tools for the analysis of Array-CGH data, but current methods are only based on first-order HMMs having constrained abilities to model spatial dependencies between measurements of closely adjacent chromosomal regions. Here, we develop parsimonious higher-order HMMs enabling the interpolation between a mixture model ignoring spatial dependencies and a higher-order HMM exhaustively modeling spatial dependencies. We apply parsimonious higher-order HMMs to the analysis of Array-CGH data of the accessions C24 and Col-0 of the model plant Arabidopsis thaliana. We compare these models against first-order HMMs and other existing methods using a reference of known deletions and sequence deviations. We find that parsimonious higher-order HMMs clearly improve the identification of these polymorphisms. Moreover, we perform a functional analysis of identified polymorphisms revealing novel details of genomic differences between C24 and Col-0. Additional model evaluations are done on widely considered Array-CGH data of human cell lines indicating that parsimonious HMMs are also well-suited for the analysis of non-plant specific data. All these results indicate that parsimonious higher-order HMMs are useful for Array-CGH analyses. An implementation of parsimonious higher-order HMMs is available as part of the open source Java library Jstacs (www.jstacs.de/index.php/PHHMM)

Ways of Optimizing Medical Services for Children at Educational Institutions Under the Health Care System Reform in Ukraine.

Aim – scientific substantiation of the ways to optimize preventive childhood medicine under the health care system reform in Ukraine. The medical, sanitary and epidemiological conditions of children's stay were studied at 147 schools in five oblasts of Ukraine and the city of Kyiv. The following methods were used: system analysis, analytical, mathematical and statistical, bibliosemantic, modeling and expert assessments. The results of an expert assessment (224 specialists) on the optimization of medical services for schoolchildren were analyzed. The main risk factors for schoolchildren staying at educational institutions were identified: daily routine failure, non-compliance with the hygiene requirements for the premises where the educational process is carried out, as well as failure in food and water quality and safety. The levels of medical observation provision were analyzed. There were suggested two ways of maintaining the role of medical personnel in preserving and enhancing the health of schoolchildren: involving doctors of primary care centers of the Ministry of Health of Ukraine and creating an autonomous medical service in the institutions of the Ministry of Education and Science of Ukraine in accordance with the current licensing requirements. The issues of prevention and promotion of children's health, including schoolchildren, are one of the key aspects of the training of medical personnel at all stages. Such programs need to be modernized on the basis of a competency approach, taking into account the current needs of the health care system and the best International experience in resolving these issues

Prospects for Healthcare Personnel Training in the Public Health System of Ukraine

Purpose: scientific substantiation of training of medical personnel for the public health system of Ukraine. During 2017-2020, the Department of Public Health conducted a systematic analysis of public health staffing in Ukraine, developed countries and Europe. For the first time, a model of formation of specialists' competencies for the public health system of Ukraine in the postgraduate education program with a multidisciplinary approach to training is substantiated and developed, which includes such disciplines as epidemiology, hygiene, medical statistics, laboratory diagnostics, information technology, legal law and others. This enables to reveal the tools and methods of system management, improve the availability and quality of work during the formation and development of the industry. The need for medical education for public health specialists who are ready to perform basic operational functions to preserve and strengthen the health of the population of Ukraine has been established. For the first time, methodological approaches to the improvement of higher medical education at the undergraduate and postgraduate levels have been scientifically substantiated, taking into account modern requirements for the functioning of the public health system; competence models of the public health specialist in modern conditions of realization of his activity are created; standards of higher education at the first (bachelor's) and second (master's) levels in the specialty 229 “Public health” in the field of knowledge 22 “Health Care” have been developed