The anatomical components of the cardiac outflow tract of the bichir, polypterus senegalus. Evolutionary significance

El resumen aparece en el Program & Abstracts of the 10th International Congress of Vertebrate Morphology, Barcelona 2013.Anatomical Record, Volume 296, Special Feature — 1: P-077.In chondrichthyans and actinopterygians, the outflow tract of the heart, namely, the cardiac portion intercalated between the ventricle and the ventral aorta, consists of two anatomical components: conus arteriosus and bulbus arteriosus. In chondrichthyans and extant representatives of phylogenetically ancient actinopterygian groups, the conus and bulbus are well-developed in size, whereas in most teleosts, the bulbus is markedly larger than the conus. Current knowledge about the cardiac outflow tract of the polypteriformes is scarce and highly contradictory, a fact that contrasts with their crucial phylogenetic position at the source of the actinopterygian lineage. In fact, it remains uncertain whether they have a bulbus at the arterial pole of the heart. The present study aimed to elucidate the anatomical arrangement of the cardiac outflow tract of the bichir in an attempt to improve our understanding of the evolution of the vertebrate heart. We examined the hearts from 12 bichirs using histochemical and immunohistochemical techniques. Our findings showed that the outflow tract of the bichir consists of two components, namely, a long conus arteriosus, largely composed of myocardial tissue and furnished with a variable number of valves at its luminal side, and a very short, elastin rich bulbus arteriosus, devoid of myocardium. The bulbus has an arterial-like histological composition. However, it differs from the aorta because it has a thicker wall, shows a different arrangement of the histological elements, is covered by the epicardium and is crossed by coronary arteries. The present observations are consistent with the notion that the conus arteriosus and the bulbus arteriosus have coexisted from the beginning of the jawed vertebrate radiation. This is of particular interest, because there is evidence that the bulbus arteriosus, which is a second heart field derivative, is homologous with the intrapericardial portions of the aorta and pulmonary artery of birds and mammals.Proyecto CGL2010-16417/BOS; Fondos FEDER

Construction of a self-luminescent cyanobacterial bioreporter that detects a broad range of bioavailable heavy metals in aquatic environments

Frontiers in Microbiology 6 (2015): 186 This Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permissionA self-luminescent bioreporter strain of the unicellular cyanobacterium Synechococcus sp. PCC 7942 was constructed by fusing the promoter region of the smt locus (encoding the transcriptional repressor SmtB and the metallothionein SmtA) to luxCDABE from Photorhabdus luminescens; the sensor smtB gene controlling the expression of smtA was cloned in the same vector. The bioreporter performance was tested with a range of heavy metals and was shown to respond linearly to divalent Zn, Cd, Cu, Co, Hg, and monovalent Ag. Chemical modeling was used to link bioreporter response with metal speciation and bioavailability. Limits of Detection (LODs), Maximum Permissive Concentrations (MPCs) and dynamic ranges for each metal were calculated in terms of free ion concentrations. The ranges of detection varied from 11 to 72 pM for Hg2+ (the ion to which the bioreporter was most sensitive) to 1.54-5.35 μM for Cd2+ with an order of decreasing sensitivity as follows: Hg2+ >> Cu2+ >> Ag+ > Co2+ = Zn2+ > Cd2+. However, the maximum induction factor reached 75-fold in the case of Zn2+ and 56-fold in the case of Cd2+, implying that Zn2+ is the preferred metal in vivo for the SmtB sensor, followed by Cd2+, Ag+ and Cu2+ (around 45-50-fold induction), Hg2+ (30-fold) and finally Co2+ (20-fold). The bioreporter performance was tested in real environmental samples with different water matrix complexity artificially contaminated with increasing concentrations of Zn, Cd, Ag, and Cu, confirming its validity as a sensor of free heavy metal cations bioavailability in aquatic environmentsThis study was funded by MINECO grants CGL2010-15675 and CTM2013-45775-C2-2-

Factores condicionantes de la sostenibilidad como una dimensión estratégica del desarrollo turístico mexicano

This paper proposes the Delphi analysis to identify and analyze the key factors of sustainability in Mexicoʼs present tourism-development model. The importance of the results is due to the fact that sustainability has become a central aspect in the competitiveness of any tourism destination. In this sense, Mexicoʼs current shortfalls may limit the role of tourism in the economic development of the country. So, the definition of the sustainability factors in Mexico leads to the design and implementation of strategies to overcome such situation.A través del análisis Delphi, en este trabajo se identifican y analizan los principales condicionantes de la sostenibilidad del actual modelo de desarrollo turístico mexicano. La importancia de los resultados obtenidos radica en que la sostenibilidad se ha convertido en un factor clave en la capacidad de competir de cualquier destino turístico, y México presenta actualmente serios déficit en este aspecto, lo que, a su vez, puede constreñir la contribución del turismo al desarrollo económico del país. De ahí que conocer los factores que condicionan la sostenibilidad del turismo mexicano contribuya al diseño e implementación de estrategias para superar esta situación

Analysis of chiral effects in non-planar metamaterials with oblique incidence

One of the main research lines of the Grupo de Electromagnetismo of the Universidad de Cantabria is focused on the study and characterization of planar chiral metamaterial (CMM) structures implemented on printed circuit board technology. The objective of this communication is to analyze the influence of the angle of incidence on the chiral behavior of metamaterial structures. A variation of the split ring resonator (SRR) particle has been used as unit cell of the periodic metamaterials analyzed. From this structure, the effect of the angle of incidence on the chiral response is analyzed, evaluating variations in the geometry that provide different types of interaction between unit cells or between the incident wave and the structure. The SRRs are printed only on one side of the printed circuit board. Fig.1. This way, with normal incidence the structure is achiral. The obtained results show that the angle of incidence is a key parameter for the structure in order to present a chiral behavior. In this case, the chiral behavior is shown as a high difference between the transmission levels of the two eigenwaves (left handed and right handed circular polarized waves), i.e., circular dichroism

Radon concentration in caves as a proxy for tectonic activity in the cantabrian mountains (Spain)

Radon (Rn) constitutes a good geochemical tracer for neotectonic activity in faults since associated fracturing near the surface favours fluid escape to the atmosphere. In this contribution, we measured the Rn concentration in the air inside karst caves to constraints the recent fault activity in the Cantabrian Mountains (N Spain). Rock formations exhumed during the uplifting of the Cantabrian Mountains record a long history of fracturing, which has the potential to connect deeper sources of Rn with the surface. In this regional study, we correlate Rn measurements with cave survey data and geological structures using a Geographic Information Systems. Thirty-four Rn average concentration was recorded by CR-39 detectors during 8 integrated months. The method is applied to the central part of the Cantabrian Mountains that is built on sedimentary and low-grade metamorphic rocks relatively poor in U. Dominant tectonic structures and Rn concentration are examined in 28 cavities. The concentration of Rn values is higher than 0.5 kBq·m-3 in caves developed preferably following fractures with the direction N30oW, being the concentration greater than 0.8 kBq·m-3 in cavities located less than 200±50 m from subvertical faults with such orientation. Rn anomalies point to relative high connectivity along subvertical fault zones NW-trending, preserving fracture connectivity in the most recent structures in the Cantabrian Mountains. Finally, in the study area there is a low but significant radioactive hazard which is associated to fault zones in a fractured rock massif. It contrasts with other active tectonic settings where the radioactive hazard may come from fault movements

Methodological Approaches to Radon in Water Measurements: Comparative Experiences between Romania and Spain

The EC Directive 2013/51/EURATOM of 22 October 2013 represents the first time that a radon limit for drinking water has been proposed. Transposition of this Directive into Spanish legislation was by means of the recent RD 314/2016 which sets a limit value of 500 Bq l?1 for radon (222Rn) in water for human consumption while that in Romanian legislation the Law 301/2015 provides a limit value of 100 Bq l-1 for 222Rn concentration in water for human consumption as well as a total effective dose of 0.1 mSv y-1 from all radionuclides present in water (same dose value established by Spanish law). For several years, both in Romania and in Spain, there have been campaigns to measure the concentration of 222Rn in waters of different origins in order to determine the possible exposure of the population through this irradiation path, and also to use the presence of radon dissolved in water as a source of indoor radon in thermal spa facilities, and also as a tracer of dynamic processes (C. Sainy et al., Sci. Tot. Envir. 543, 460 (2016); 1). In this article, an assessment of the results from measurement campaigns in both countries will be presented and compared. In addition, the different measurement techniques used will be described, and the description of an intercomparison exercise on radon measurement in water organized by the Environmental Radioactivity Laboratory of the University of Cantabria (LaRUC) will be presented, in order to evaluate the quality of the performance of the techniques used.This work was partially supported by the project ID P_37_229, Contract No. 22/01.09.2016, with the title Smart Systems for Public Safety through Control and Mitigation of Residential Radon linked with Energy Efficiency Optimization of Buildings in Romanian Major Urban Agglomerations SMART-RAD-EN of the POC Programme

Effects of Geometry in Atrial Fibrillation Markers Obtained With Electrocardiographic Imaging

[EN] Electrocardiographic imaging (ECGI) can characterise cardiac pathologies such as atrial fibrillation (AF) through specific markers based on frequency or phase analysis. In this study, the effect of the geometry of patients torso and atria in the ECGI resolution is studied. A realistic 3D atrial geometry was located on 30 patient torsos and ECGI signals were calculated for 30 different AF simulations in each torso. Dominant frequency (DF) and reentrant activity analysis were calculated for each scenario. Anatomical and geometrical measurements of each torso (30-80% of variability between patients) and atria were calculated and compared with the errors in the ECGI estimation versus the departing EGM maps. Results show evidences that big chest dimensions worsen the non-invasive calculation of AF markers (p<0.05). Also, higher number of visible electrodes from each atrial region improves ECGI characterization measured as lower DF deviations (0.64±0.26 Hz vs 0.72±0.27 Hz, p<0.05) and higher reentrant activity coincidence (10.1±12.2% vs 3.4±3.4%, p<0.05). Torso and atrial geometry affect the quality of the non-invasive reconstruction of AF markers such as DF or reentrant activity. Knowing the geometrical parameters that worsen non-invasive AF maps may help to measure each detected AF driver reliability.Supported in part by: Instituto de Salud Carlos III FEDER (Fondo Europeo de Desarrollo Regional; IJCI2014-22178, DTS16/00160; PI14/00857, PI16/01123; PI17/01059; PI17/01106), Generalitat Valenciana Grants (APOSTD/2017 and APOSTD/2018) and projects (GVA/2018/103) and EIT-Health 19600 AFFINE.Molero-Alabau, R.; Climent, AM.; Hernández-Romero, I.; Liberos, A.; Fernández-Avilés, F.; Atienza, F.; Guillem Sánchez, MS.... (2019). Effects of Geometry in Atrial Fibrillation Markers Obtained With Electrocardiographic Imaging. IEEE. 1-4. https://doi.org/10.22489/CinC.2019.3081

Spanish experience on the design of radon surveys based on the use of geogenic information

One of the requirements of the recently approved EU-BSS (European Basic Safety Standards Directive, EURATOM, 2013) is the design and implementation of national radon action plans in the member states (Annex XVIII). Such plans require radon surveys. The analysis of indoor radon data is supported by the existing knowledge about geogenic radiation. With this aim, we used the terrestrial gamma dose rate data from the MARNA project. In addition, we considered other criterion regarding the surface of Spain, population, permeability of rocks, uranium and radium contain in soils because currently no data are available related to soil radon gas concentration and permeability in Spain. Given that, a Spanish radon map was produced which will be part of the European Indoor Radon Map and a component of the European Atlas of Natural Radiation. The map indicates geographical areas with high probability of finding high indoor radon concentrations. This information will support legislation regarding prevention of radon entry both in dwellings and workplaces. In addition, the map will serve as a tool for the development of strategies at all levels: individual dwellings, local, regional and national administration

Solving Inaccuracies in Anatomical Models for Electrocardiographic Inverse Problem Resolution by Maximizing Reconstruction Quality

[EN] Electrocardiographic Imaging has become an increasingly used technique for non-invasive diagnosis of cardiac arrhythmias, although the need for medical imaging technology to determine the anatomy hinders its introduction in the clinical practice. This paper explores the ability of a new metric based on the inverse reconstruction quality for the location and orientation of the atrial surface inside the torso. Body surface electrical signals from 31 realistic mathematical models and four AF patients were used to estimate the optimal position of the atria inside the torso. The curvature of the L-curve from the Tikhonov method, which was found to be related to the inverse reconstruction quality, was measured after application of deviations in atrial position and orientation. Independent deviations in the atrial position were solved by finding the maximal L-curve curvature with an error of 1.7 +/- 2.4 mm in mathematical models and 9.1 +/- 11.5 mm in patients. For the case of independent angular deviations, the error in location by using the L-curve was 5.8 +/- 7.1 degrees in mathematical models and 12.4 degrees +/- 13.2 degrees in patients. The ability of the L-curve curvature was tested also under superimposed uncertainties in the three axis of translation and in the three axis of rotation, and the error in location was of 2.3 +/- 3.2 mm and 6.4 degrees +/- 7.1 degrees in mathematical models, and 7.9 +/- 10.7 mm and 12.1 degrees +/- 15.5 degrees in patients. The curvature of L-curve is a useful marker for the atrial position and would allow emending the inaccuracies in its location.This work was supported in part by Generalitat Valenciana under Grant ACIF/2013/021, in part by the Instituto de Salud Carlos III, Ministry of Economy and Competitiveness, Spain, under Grant PI13-01882, Grant PI13-00903, Grant PI14/00857, Grant TEC2013-46067-R, and Grant DTS16/00160, in part by the Spanish Society of Cardiology (Grant for Clinical Research in Cardiology 2015), and in part by the Spanish Ministry of Science and Innovation (Red RIC) under Grant PLE2009-0152.Rodrigo Bort, M.; Climent, AM.; Liberos Mascarell, A.; Hernández-Romero, I.; Arenal, A.; Bermejo, J.; Fernández-Avilés, F.... (2018). Solving Inaccuracies in Anatomical Models for Electrocardiographic Inverse Problem Resolution by Maximizing Reconstruction Quality. IEEE Transactions on Medical Imaging. 37(3):733-740. https://doi.org/10.1109/TMI.2017.2707413S73374037