Trend analysis for daily rainfall series of Barcelona

Frequency analysis of hydrological series is a key point to acquire an in-depth understanding of the behaviour of hydrologic events. The occurrence of extreme hydrologic events in an area may imply great social and economical impacts. A good understanding of hazardous events improves the planning of human activities.Peer ReviewedPostprint (published version

Use of Sensors in the Treatment and Follow-up of Patients with Diabetes Mellitus

Glucose control is the cornerstone of Diabetes Mellitus (DM) treatment. Although self-regulation using capillary glycemia (SRCG) still remains the best procedure in clinical practice, continuous glucose monitoring systems (CGM) offer the possibility of continuous and dynamic assessment of interstitial glucose concentration. CGM systems have the potential to improve glycemic control while decreasing the incidence of hypoglycemia but the efficiency, compared with SRCG, is still debated. CGM systems have the greatest potential value in patients with hypoglycemic unawareness and in controlling daily fluctuations in blood glucose. The implementation of continuous monitoring in the standard clinical setting has not yet been established but a new generation of open and close loop subcutaneous insulin infusion devices are emerging making insulin treatment and glycemic control more reliable

Impact of interstitial lung disease on the survival of systemic sclerosis with pulmonary arterial hypertension

To assess severity markers and outcomes of patients with systemic sclerosis (SSc) with or without pulmonary arterial hypertension (PAH-SSc/non-PAH-SSc), and the impact of interstitial lung disease (ILD) on PAH-SSc. Non-PAH-SSc patients from the Spanish SSc registry and PAH-SSc patients from the Spanish PAH registry were included. A total of 364 PAH-SSc and 1589 non-PAH-SSc patients were included. PAH-SSc patients had worse NYHA-functional class (NYHA-FC), worse forced vital capacity (FVC) (81.2 +/- 20.6% vs 93.6 +/- 20.6%, P < 0.001), worse tricuspid annular plane systolic excursion (TAPSE) (17.4 +/- 5.2 mm vs 19.9 +/- 6.7 mm, P < 0.001), higher incidence of pericardial effusion (30% vs 5.2%, P < 0.001) and similar prevalence of ILD (41.8% vs. 44.9%). In individuals with PAH-SSc, ILD was associated with worse hemodynamics and pulmonary function tests (PFT). Up-front combination therapy was used in 59.8% and 61.7% of patients with and without ILD, respectively. Five-year transplant-free survival rate was 41.1% in PAH-SSc patients and 93.9% in non-PAH-SSc patients (P < 0.001). Global survival of PAH-SSc patients was not affected by ILD regardless its severity. The multivariate survival analysis in PAH-SSc patients confirmed age at diagnosis, worse NYHA-FC, increased PVR, reduced DLCO, and lower management with up-front combination therapy as major risk factors. In conclusion, in PAH-SSc cohort risk of death was greatly increased by clinical, PFT, and hemodynamic factors, whereas it was decreased by up-front combination therapy. Concomitant ILD worsened hemodynamics and PFT in PAH-SSc but not survival regardless of FVC impairment

Integrative epigenomics in Sjögren´s syndrome reveals novel pathways and a strong interaction between the HLA, autoantibodies and the interferon signature

Primary Sjögren's syndrome (SS) is a systemic autoimmune disease characterized by lymphocytic infiltration and damage of exocrine salivary and lacrimal glands. The etiology of SS is complex with environmental triggers and genetic factors involved. By conducting an integrated multi-omics study, we confirmed a vast coordinated hypomethylation and overexpression effects in IFN-related genes, what is known as the IFN signature. Stratified and conditional analyses suggest a strong interaction between SS-associated HLA genetic variation and the presence of Anti-Ro/SSA autoantibodies in driving the IFN epigenetic signature and determining SS. We report a novel epigenetic signature characterized by increased DNA methylation levels in a large number of genes enriched in pathways such as collagen metabolism and extracellular matrix organization. We identified potential new genetic variants associated with SS that might mediate their risk by altering DNA methylation or gene expression patterns, as well as disease-interacting genetic variants that exhibit regulatory function only in the SS population. Our study sheds new light on the interaction between genetics, autoantibody profiles, DNA methylation and gene expression in SS, and contributes to elucidate the genetic architecture of gene regulation in an autoimmune population

Serum profiling identifies CCL8, CXCL13, and IL-1RA as markers of active disease in patients with systemic lupus erythematosus

IntroductionSystemic lupus erythematosus (SLE) is a clinically heterogeneous disease that presents a challenge for clinicians. To identify potential biomarkers for diagnosis and disease activity in SLE, we investigated a selected yet broad panel of cytokines and autoantibodies in patients with SLE, healthy controls (HC), and patients with other autoimmune diseases (AIDs).MethodsSerum samples from 422 SLE patients, 546 HC, and 1223 other AIDs were analysed within the frame of the European PRECISESADS project (NTC02890121). Cytokine levels were determined using Luminex panels, and autoantibodies using different immunoassays.ResultsOf the 83 cytokines analysed, 29 differed significantly between patients with SLE and HC. Specifically, CCL8, CXCL13, and IL-1RA levels were elevated in patients with active, but not inactive, SLE versus HC, as well as in patients with SLE versus other AIDs. The levels of these cytokines also correlated with SLE Disease Activity Index 2000 (SLEDAI-2K) scores, among five other cytokines. Overall, the occurrence of autoantibodies was similar across SLEDAI-2K organ domains, and the correlations between autoantibodies and activity in different organ domains were weak.DiscussionOur findings suggest that, upon validation, CCL8, CXCL13, and IL-1RA could serve as promising serum biomarkers of activity in SLE

O31 Integrative analysis reveals a molecular stratification of systemic autoimmune diseases

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Host plant use by two distinct lineages of the tomato red spider mite, Tetranychus evansi, differing in their distribution range

International audienceThe tomato red spider mite, Tetranychus evansi, is an emerging pest of solanaceous crops. Two distinct genetic lineages (I and II) have been identified, lineage I having a much wider geographic distribution than lineage II. This has been attributed to differences in cold hardiness that make lineage I better adapted to colonize the coldest parts of the invaded area. However, other factors such as the ability to exploit different hosts may also be involved. In this work, we compared the performance of the Nice (lineage I) and Perpignan (lineage II) strains of T. evansi on two frequent host plants for this species: black nightshade, Solanum nigrum, and cultivated tomato, S. lycopersicum. In general, Nice strain mites performed better (higher fecundity, lower offspring mortality, bigger egg size and lower percentage of males) than Perpignan strain mites when both: (1) they were reared and tested on the same host plant (S. lycopersicum or S. nigrum); and (2) when shifted from S. nigrum to S. lycopersicum and vice versa. Digestive proteases showed also higher expression in Nice strain mites than in Perpignan strain mites, independently of their plant host, potentially reflecting a more efficient proteolytic digestion of plant proteins. However, no differences in detoxification enzyme (P450, esterases and glutathione S-transferases) activities were found when the two strains were compared. In conclusion, our results demonstrate that Nice strain mites exhibited life history traits leading to higher fitness on two different hosts, which may be related with the higher invasive potential and outbreak risks of mites from lineage I

Phytocystatins: Defense Proteins against Phytophagous Insects and Acari

This review deals with phytocystatins, focussing on their potential role as defence proteins against phytophagous arthropods. Information about the evolutionary, molecular and biochemical features and inhibitory properties of phytocystatins are presented. Cystatin ability to inhibit heterologous cysteine protease activities is commented on as well as some approaches of tailoring cystatin specificity to enhance their defence function towards pests. A general landscape on the digestive proteases of phytophagous insects and acari and the remarkable plasticity of their digestive physiology after feeding on cystatins are highlighted. Biotechnological approaches to produce recombinant cystatins to be added to artificial diets or to be sprayed as insecticide–acaricide compounds and the of use cystatins as transgenes are discussed. Multiple examples and applications are included to end with some conclusions and future perspectives

Trend analysis for daily rainfall series of Barcelona

Frequency analysis of hydrological series is a key point to acquire an in-depth understanding of the behaviour of hydrologic events. The occurrence of extreme hydrologic events in an area may imply great social and economical impacts. A good understanding of hazardous events improves the planning of human activities.Peer Reviewe

Bayesian trend analysis for daily rainfall series of Barcelona

A Point-Over-Threshold approach using a reparameterization of the Generalized Pareto Distribution (GPD) has been used to assess changes in the daily rainfall Barcelona series (1854–2006). A Bayesian approach, considering the suitable scale and physical features of the phenomenon, has been used to look for these alterations. Two different models have been assessed: existence of abrupt changes in the new GPD parameters due to modifications of the observatory locations and trends in these GPD parameters, pointing to a climate change scenario