Bioinformatic analysis and purification of glutathione transferase (GST) from Pseudomonas sp. UW4

The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search at UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative glutathione transferases for the microorganism. The molecular weights of the isoforms ranged from 17.6 to 34.06 kDa. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutathione (BSP) affinity column, resolved into a single band with a low molecular weight (MW) of 16 kDa with the pI value of 6.0. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide, and hydrogen peroxide, but no detectable activity with Trans-2-octenal, hepta-2,4-dienal and Trans-4-phenyl-3-butene-2-one. This has proven that putative GST possessed peroxidase activity and proposed to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4 according to its estimated molecular weight and the pI values obtained experimentally

Bioinformatic analysis and purification of Glutathione Transferase (GST) from Pseudomonas sp. UW4

The study aimed at identifying and purifying cytosolic glutathione transferase isoforms expressed in Pseudomonas sp. UW4. Search at UniProt (https://www.uniprot.org/uniprot/), has indicated that there were 20 genes encoding putative glutathione transferases for the microorganism. The molecular weights of the isoforms ranged from 17.6 to 34.06 kDa. SDS-polyacrylamide gel electrophoresis revealed that the GST purified using Sulfobromophthalein-glutathione (BSP) affinity column, resolved into a single band with a low molecular weight (MW) of 16 kDa with the pI value of 6.0. Purified GST was reactive towards ethacrynic acid, 1-chloro-2,4-dinitrobenzene, cumene hydroxide, and hydrogen peroxide, but no detectable activity with Trans-2-octenal, hepta-2,4-dienal and Trans-4-phenyl-3-butene-2-one. This has proven that putative GST possessed peroxidase activity and proposed to be similar to PputUW4_00801 (putative glutathione S-transferase) of Pseudomonas sp. UW4 according to its estimated molecular weight and the pI values obtained experimentally

High Mutational Heterogeneity, and New Mutations in the Human Coagulation Factor V Gene. Future Perspectives for Factor V Deficiency Using Recombinant and Advanced Therapies

Enfermedad de Owren; Análisis de mutaciones; ParahemofiliaMalaltia d'Owren; Anàlisi de mutacions; ParahemofíliaOwren’s disease; Mutation analysis; ParahemophiliaFactor V is an essential clotting factor that plays a key role in the blood coagulation cascade on account of its procoagulant and anticoagulant activity. Eighty percent of circulating factor V is produced in the liver and the remaining 20% originates in the α-granules of platelets. In humans, the factor V gene is about 80 kb in size; it is located on chromosome 1q24.2, and its cDNA is 6914 bp in length. Furthermore, nearly 190 mutations have been reported in the gene. Factor V deficiency is an autosomal recessive coagulation disorder associated with mutations in the factor V gene. This hereditary coagulation disorder is clinically characterized by a heterogeneous spectrum of hemorrhagic manifestations ranging from mucosal or soft-tissue bleeds to potentially fatal hemorrhages. Current treatment of this condition consists in the administration of fresh frozen plasma and platelet concentrates. This article describes the cases of two patients with severe factor V deficiency, and of their parents. A high level of mutational heterogeneity of factor V gene was identified, nonsense mutations, frameshift mutations, missense changes, synonymous sequence variants and intronic changes. These findings prompted the identification of a new mutation in the human factor V gene, designated as Jaén-1, which is capable of altering the procoagulant function of factor V. In addition, an update is provided on the prospects for the treatment of factor V deficiency on the basis of yet-to-be-developed recombinant products or advanced gene and cell therapies that could potentially correct this hereditary disorder.This study was supported by the Andalusian Association of Hemophilia (ASANHEMO FV 2016–20 grant) and Octapharma S.A. (OCPH-2019-20 grant)

Promoting social capital, self-management and health literacy in older adults through a group-based intervention delivered in low-income urban areas : results of the randomized trial AEQUALIS

Altres ajuts: Recercaixa (2014ACUP00207)Background: Evidence is scarce on how to promote health and decrease cumulative inequalities for disadvantaged older people. Downstream complex interventions focusing on intermediate factors (self-management, health literacy and social capital) may have the potential to mitigate the inequitable impacts of social determinants in health. The aim of the AEQUALIS study was to assess the effectiveness of a group-based intervention to improve self-perceived health as indicator of health inequality. Methods: Pragmatic randomised clinical trial addressed to older adults (≥ 60 years) living in urban disadvantaged areas with low self-perceived health. The intervention was delivered in primary care settings and community assets between 2015 and 2017 and consisted in 12 weekly sessions. The primary outcome was self-perceived health assessed in two ways: with the first item of the SF-12 questionnaire, and with the EQ-5D visual analog scale. Secondary outcomes were health-related quality of life, social capital, self-management, mental health and use of health services. Outcomes were assessed at baseline, post intervention and follow-up at 9 months after the end of the intervention. Results: 390 people were allocated to the intervention group (IG) or the control group (CG) and 194 participants and 164 were included in the data analysis, respectively. Self perceived health as primary outcome assessed with SF-12-1 was not specifically affected by the intervention, but with the EQ-5D visual analog scale showed a significant increase at one-year follow-up only in the IG (MD=4.80, 95%CI [1.09, 8.52]). IG group improved health literacy in terms of a better understanding of medical information (− 0.62 [− 1.10, − 0.13]). The mental component of SF-12 improved (3.77 [1.82, 5.73]), and depressive symptoms decreased at post-intervention (− 1.26 [− 1.90, − 0.63]), and at follow-up (− 0.95 [− 1.62, − 0.27]). The use of antidepressants increased in CG at the follow-up (1.59 [0.33, 2.86]), while it remained stable in the IG. Conclusions: This study indicates that a group intervention with a strong social component, conducted in primary health care and community assets, shows promising effects on mental health and can be used as a strategy for health promotion among older adults in urban disadvantaged areas. Trial registration: ClinicalTrials.gov, NCT02733523. Registered 11 April 2016 - Retrospectively registere

Motivación de logro para aprender en educación física. Adaptación de la versión española del Test AMPET

El presente estudio ha tenido como principal objetivo la adaptación de la versión española del Test de Motivación de Logro para el Aprendizaje en Educación Física (AMPET). Esta adaptación supuso la inclusión de una nueva dimensión que permitiera que la competencia motriz percibida pudiera ser analizada desde dos perspectivas, una autopercibida y la comparada con los demás. Se presentan dos estudios. En el primero mediante AFE se exploró la estructura factorial de 4 dimensiones prevista teóricamente. El segundo estudio sirvió para confirmar el modelo mediante un AFC que ofreció cuatro factores, buen ajuste y coeficientes de fiabilidad iguales o superiores a .70 en cada una de las dimensiones obtenidas

Motivación de Logro para Aprender en Educación Física: Adaptación de la versión española del Test AMPET E

El presente estudio ha tenido como principal objetivo la adaptación de la versión española del Test de Motivación de Logro para el Aprendizaje en Educación Física (AMPET). Esta adaptación supuso la inclusión de una nueva dimensión que permitiera que la competencia motriz percibida pudiera ser analizada desde dos perspectivas, una autopercibida y la comparada con los demás. Se presentan dos estudios. En el primero mediante AFE se exploró la estructura factorial de 4 dimensiones prevista teóricamente. El segundo estudio sirvió para confirmar el modelo mediante un AFC que ofreció cuatro factores, buen ajuste y coeficientes de fiabilidad iguales o superiores a .70 en cada una de las dimensiones obtenidas

Training needs of university professors in skills related to the tutoring action

[ES] El objeto de este estudio es determinar el grado de capacitación del profesorado frente a las competencias que se consideran básicas para el desempeño de la acción tutorial en la universidad. De esta forma se puede valorar cuáles son los puntos fuertes y cuáles son deficitarios para la elaboración de futuros programas de formación del profesorado frente al desempeño de la nueva función tutorial dentro del EEEs.  En esta investigación participaron 279 profesores de tres universidades andaluzas de las diversas facultades o áreas de conocimiento, empleándose para tal selección un muestreo probabilístico, de carácter aleatorio por afijación proporcional en función de los diferentes departamentos que componen cada facultad, enviando el cuestionario a cada uno de los sujetos seleccionados mediante e mail a su correo institucional, recogiendo únicamente los resultados de las personas que accedieron a responder. Los resultados obtenidos permiten aclarar cuáles son las competencias que más pose[EN] The purpose of this study is to determine the degree of training of teachers against the competencies that are considered essential   for   the   performance   of   the tutoring   action   in   the   university.   So that there could be valued which are the  strong  points  and  which  are  deficit for  the  production  of  futures  programs of    formation    of    the    professorship opposite to the performance of the new tutoring function inside the EHEA. In this investigation there took part 279 teachers from three Andalusian universities of the diverse  powers  or  knowledge ́s  areas.  A probability sampling being used for such a selection, of random nature by affixation proportional  depending  on  the  different departments that compose every faculty, sending the questionnaire to each of the subjects selected by means of and e-mail to  his  institutional  mail,  gathering  only the results of the persons who agreed to answer. The obtained results allow to clarify which are theAguilar Parra, JM.; Alias García, A.; Fernández Campoy, JM.; Álvarez, J.; Pérez Gallardo, ER.; Hernández Rodríguez, AI. (2015). Necesidades de formación del profesor universitario en competencias relacionadas con la acción tutorial. REDU. Revista de Docencia Universitaria. 13(3):357-375. https://doi.org/10.4995/redu.2015.5433OJS357375133Aguilar, J. M. y Martínez, M. (2012). Modalidades de tutoría. En Álvarez, J. Plan de Orientación para la Universidad. Madrid: Editorial Académica Española.Álvarez-Pérez, P. R. (2002). La función tutorial en la universidad. Una apuesta por la mejora de la calidad de la enseñanza. Madrid: EOS.Álvarez, V., García, E., Gil, J. y Romero, S. (2004). La enseñanza universitaria. Planificación y desarrollo de la docencia. Madrid: EOS.Álvarez-Rojo, V., Romero, S., Gil-Flores, J., Rodríguez-Santero, J., Clares, J., Asensio, I., Del-Frago, R. y Salmerón-Vílchez, P. (2011). Necesidades de formación del profesorado universitario para la adaptación de su docencia al Espacio Europeo de Educación Superior (EEES). Relieve, 17(1), 1-22.Casado, R., Greca, I. M., Tricio, V., Collado, M. y Lara, A. M. (2014). Impacto de un Plan de Acción Tutorial universitario: resultados académicos, implicación y satisfacción. REDU - Revista de Docencia Universitaria, 12(4), 323-342.Castaño, E., Blanco, A. y Asensio, E. (2012). Competencias para la tutoría: experiencia de formación con profesores universitarios. REDU - Revista de Docencia Universitaria, 10(2), 193-210.Comellas, M. J. (Coord.) (2002). Las competencias del profesorado para la acción tutorial. Barcelona: Cisspraxis.De Pablos, J. (Coord.) (2006). El proceso de integración en el Espacio Europeo de Educación Superior: Necesidades y demandas del profesorado de la Universidad de Sevilla.Sevilla: Secretariado de Publicaciones de la Universidad de Sevilla.Domínguez, G., Álvarez, F. J. y López, A. M. (2013). Acción tutorial y orientación en el periodo de transición de la Educación Secundaria a la Universidad. La orientación al alumnado de nuevo ingreso. REDU - Revista de Docencia Universitaria, 11(2), 221-241.Fernández, F. y Arco, J. (2011). Efectos de un programa de acción tutorial entre universitarios. Infancia y Aprendizaje, 34(1), 109-122.Gallego, S. (1997). Las funciones del tutor universiraio. En AEOP (Comp.), La orientación educativa y la intervención psicopedagógica integradas en el currículum. Valencia: AEOP.Gil-Albarova, A., Martínez, O., Tunnicliffe, A. y Moneo, J. M. (2013). Estudiantes universitarios y calidad de plan de acción tutorial. Valoraciones y mejoras. REDU - Revista de Docencia Universitaria, 11(2), 63-87.González-Sanmamed, M. (Dir.) (2006). O EEES: perspectiva do profesorado das universidades galegas. Santiago de Compostela: ACSUG.González-Sanmamed, M. y Raposo, M. (2008). Necesidades formativas del profesorado universitario en el contexto de la convergencia europea. Revista de Investigación Educativa, 26(2), 285-306.González-Sanmamed, M. y Raposo, M. (2009). Valoraciones del profesorado universitario sobre las acciones que pueden favorecer el proceso de convergencia europea.Revista de Educación, 349, 361-390.Lucas, S. (2007). Desarrollo de competencias desde la enseñanza universitaria. Armonización con la Educación Secundaria y el mercado de trabajo, desde la Psicología Social de la Educación. Electronic Journal of Research in Educational Psychology, 11(5), 125-158.Margalef, L. y Álvarez, J. M. (2005). La formación del profesorado universitario para la innovación en el marco de la integración del Espacio Europeo de Educación Superior. Revista de Educación, 337, 51-70.Monereo, C. (2010). La formación del profesorado una pauta para el análisis e intervención a través de incidentes críticos. Revista Iberoamericana de Educación, 52, 149-170.Pantoja, A. (2005). La acción tutorial en la universidad: propuestas para el cambio.Cultura y Educación, 17(1), 67-82.Rodríguez, J., Álvarez, V., Gil, J. y Romero, S. (2011). Necesidades del profesorado de la Universidad de Sevilla respecto a la enseñanza que requiere el EEES. Cultura y Educación, 23(3), 323-340.Rodríguez-Espinar, S. (2003). Nuevos retos y enfoques en la formación del profesorado universitario. Revista de Educación, 331, 67-99.Rodríguez-Moreno, Mª. L. (2002). Hacia una nueva orientación universitaria. Barcelona: Ediciones de la Universidad de Barcelona.Roelofs, E. y Sanders, P. (2007). Towards a framework for assessing teacher competence. European Journal of Vocational Training, 40(1), 123-139.Sánchez, F. (Coord.) (2005). Modelos de formación del profesorado y su valoración para el Espacio Europeo de Educación Superior. Estudios y Análisis. Madrid: MEC.Valcárcel, M. (Dir.) (2003). La preparación del profesorado universitario español para la Convergencia Europea en Educación Superior. Estudios y Análisis. Madrid: MEC.Zabalza, M. A. (2003). Competencias docentes del profesorado universitario. Calidad y desarrollo profesional. Madrid: Narcea

High Mutational Heterogeneity, and New Mutations in the Human Coagulation Factor V Gene. Future Perspectives for Factor V Deficiency Using Recombinant and Advanced Therapies

Factor V is an essential clotting factor that plays a key role in the blood coagulation cascade on account of its procoagulant and anticoagulant activity. Eighty percent of circulating factor V is produced in the liver and the remaining 20% originates in the α-granules of platelets. In humans, the factor V gene is about 80 kb in size; it is located on chromosome 1q24.2, and its cDNA is 6914 bp in length. Furthermore, nearly 190 mutations have been reported in the gene. Factor V deficiency is an autosomal recessive coagulation disorder associated with mutations in the factor V gene. This hereditary coagulation disorder is clinically characterized by a heterogeneous spectrum of hemorrhagic manifestations ranging from mucosal or soft-tissue bleeds to potentially fatal hemorrhages. Current treatment of this condition consists in the administration of fresh frozen plasma and platelet concentrates. This article describes the cases of two patients with severe factor V deficiency, and of their parents. A high level of mutational heterogeneity of factor V gene was identified, nonsense mutations, frameshift mutations, missense changes, synonymous sequence variants and intronic changes. These findings prompted the identification of a new mutation in the human factor V gene, designated as Jaén-1, which is capable of altering the procoagulant function of factor V. In addition, an update is provided on the prospects for the treatment of factor V deficiency on the basis of yet-to-be-developed recombinant products or advanced gene and cell therapies that could potentially correct this hereditary disorder

Systematic Collaborative Reanalysis of Genomic Data Improves Diagnostic Yield in Neurologic Rare Diseases

Altres ajuts: Generalitat de Catalunya, Departament de Salut; Generalitat de Catalunya, Departament d'Empresa i Coneixement i CERCA Program; Ministerio de Ciencia e Innovación; Instituto Nacional de Bioinformática; ELIXIR Implementation Studies (CNAG-CRG); Centro de Investigaciones Biomédicas en Red de Enfermedades Raras; Centro de Excelencia Severo Ochoa; European Regional Development Fund (FEDER).Many patients experiencing a rare disease remain undiagnosed even after genomic testing. Reanalysis of existing genomic data has shown to increase diagnostic yield, although there are few systematic and comprehensive reanalysis efforts that enable collaborative interpretation and future reinterpretation. The Undiagnosed Rare Disease Program of Catalonia project collated previously inconclusive good quality genomic data (panels, exomes, and genomes) and standardized phenotypic profiles from 323 families (543 individuals) with a neurologic rare disease. The data were reanalyzed systematically to identify relatedness, runs of homozygosity, consanguinity, single-nucleotide variants, insertions and deletions, and copy number variants. Data were shared and collaboratively interpreted within the consortium through a customized Genome-Phenome Analysis Platform, which also enables future data reinterpretation. Reanalysis of existing genomic data provided a diagnosis for 20.7% of the patients, including 1.8% diagnosed after the generation of additional genomic data to identify a second pathogenic heterozygous variant. Diagnostic rate was significantly higher for family-based exome/genome reanalysis compared with singleton panels. Most new diagnoses were attributable to recent gene-disease associations (50.8%), additional or improved bioinformatic analysis (19.7%), and standardized phenotyping data integrated within the Undiagnosed Rare Disease Program of Catalonia Genome-Phenome Analysis Platform functionalities (18%)

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat