Live, but at What Cost? An Analysis of Live News Reporting in the Indianapolis Television News Market

During the summer of 20 10 I had the opportunity to work as a news intern at WTHR- TV. This experience not only provided me with extremely valuable professional experience, but it also introduced me to one of the most common elements of local television news: the live shot. During my internship I would accompany reporters into the field and assist them in the news gathering process, a process that was often cut short to ensure that the reporter and videographer had an adequate amount of time to return to the news station to put together their story and then travel to a location relevant to their story for a live shot. At times, this meant that reporters had less time to gather background information about their story, speak with sources, and it even affected the amount of time they could spend writing their story. After witnessing the negative impact that live shots often have on the news gathering process, I became interested in investigating whether or not live technology capabilities are driving story-selection in newsrooms today

A method for isolating and culturing placental cells from failed early equine pregnancies

Early pregnancy loss occurs in 6–10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14–65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome™ Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL

Drivers of success in implementing sustainable tourism policies in urban areas

The existing literature in the field of sustainable tourism highlights a number of barriers that impede the implementation of policies in this area. Yet, not many studies have so far considered the factors that would contribute to putting this concept into practice, and few address the case of urban areas. The concept of sustainability has only received limited attention in urban tourism research, even though large cities are recognised as one of the most important tourist destinations that attract vast numbers of visitors. Adopting a case study approach, this paper discusses a number of drivers of success identified by policy-makers in London to contribute to the implementation of sustainable tourisms policies at the local level, and briefly looks at the relationship between these drivers and the constraints perceived by the respondents to hinder the implementation of such policies in practice. These findings may help policy-makers in other large cities to successfully develop and implement policies towards sustainable development of tourism in their area

The Development and Characterization of Tannic Acid Cross-linked Collagen Scaffolds for the Prevention of Breast Cancer Recurrence in Lumpectomy Patients

Breast cancer is the second most common cancer amongst women, second only to lung cancer. In 2014, it was estimated that there were approximately 295,000 new cases of breast cancer diagnosed in women, with approximately 40,000 deaths caused by breast cancer in the United States. It has been reported that a woman has a 12% chance of developing breast cancer in her lifetime and a 3% chance of dying from breast cancer. There are several treatment options available for breast cancer, including chemotherapy, radiation therapy, as well as two different forms of surgery, mastectomy and lumpectomy. However, none of these treatment options is perfect and there is even a chance of cancer recurrence after a patient undergoes one or more of these treatments. The long-term goal, beyond the scope of this project, is to significantly reduce the chances of cancer recurrence after a patient has undergone a lumpectomy. Currently, the chance of recurrence for a patient who undergoes a lumpectomy is around 10%. Furthermore, there is not currently an option for breast reconstruction for a lumpectomy patient. The proposed research will explore a possible approach to reducing the cancer recurrence rate as well as filling the void within the breast that was created by the lumpectomy with the patient\u27s own tissues. The approach will include the use of tannic acid (TA) as a novel cross-linking agent for collagen scaffolds that will be used to encourage the attachment and growth of the patient\u27s own cells, resulting in the regeneration of tissue to fill the void. As the cells remodel the scaffold, the tannic acid will be released into the surrounding tissue. In addition to tannic acid\u27s cross-linking abilities, tannic acid has also been shown to have anti-cancer properties which will be used to neutralize any cancer cells that may still be present within the breast. This will effectively reduce the chances of recurrence for the patient. The focus for this research was on the evaluation of an in vitro model of a lumpectomy site which will used to test the efficacy of collagen beads that have been cross-linked with tannic acid. Beads cross-linked with TA concentrations of 0.1%, 1.0% and 10.0% were evaluated during the course of these studies. These beads were first evaluated using a 2D lumpectomy model and the results showed that the 1.0% and 10.0% TA beads were able to cause a higher incidence of apoptosis in cancer cells compared to preadipocytes. The 0.1% TA beads were able to support preadipocyte cell attachment and growth throughout the duration of the studies. The 1.0% TA beads were initially able to support cell growth but after 72 hrs, the level of viable cells greatly decreased. The studies then progressed to 3D cell culture techniques using collagen/ agarose gels with cancerous cells embedded within. The gels were then exposed to the different bead types. The 1.0% and 10.0% beads were able to induce a higher rate of apoptosis in the embedded cancerous cells when compared to the control groups. The 0.1% TA beads were able to function as a viable scaffold for the duration of the 7 day study while the 1.0% TA beads were initially able to allow preadipocyte cell attachment and growth but after 4 days, the number of viable preadipocyte cells attached to the beads greatly decreased. This study was then repeated using primary cells and the same results were seen. The results of these studies showed that the ideal TA cross-linking concentration was not found. The 0.1% TA beads do not have a high enough concentration to induce apoptosis of cancerous cells while the 1.0% TA beads was too high of a concentration to allow for proper preadipocyte attachment and proliferation. Further studies of concentrations between these two end points is needed

Computational and Experimental Study of Supersonic Nozzle Flow and Shock Interactions

This study focused on the capability of NASA Tetrahedral Unstructured Software System's CFD code USM3D capability to predict the interaction between a shock and supersonic plume flow. Previous studies, published in 2004, 2009 and 2013, investigated USM3D's supersonic plume flow results versus historical experimental data. This current study builds on that research by utilizing the best practices from the early papers for properly capturing the plume flow and then adding a wedge acting as a shock generator. This computational study is in conjunction with experimental tests conducted at the Glenn Research Center 1'x1' Supersonic Wind Tunnel. The comparison of the computational and experimental data shows good agreement for location and strength of the shocks although there are vertical shifts between the data sets that may be do to the measurement technique