Nutrient Sources in the Clark Fork River Basin

Under Section 525 of the 1987 amendments to the federal Clean Water Act, Montana initiated an intensive monitoring program to identify and rank the major point and nonpoint sources of nutrients to the Clark Fork River. A 51 station monitoring network was established, including 19 stations on the Clark Fork River, 22 stations on tributary streams, and 10 municipal and industrial wastewater discharges to the river. In the first year of monitoring, samples were collected 15 times and analyzed for total and soluble forms of phosphorus and nitrogen. Several small tributaries to the upper Clark Fork (Gold, Flint, Lost, Racetrack, and Dempsey creeks and the Mill-Willow Bypass) and all 10 wastewater discharges exhibited elevated nutrient concentrations. The Missoula, Butte, and Deer Lodge municipal wastewater discharges were responsible for the largest nutrient concentrations in the Clark Fork. Inflows from good quality tributaries such as Rock Creek and the Blackfoot, Little Blackfoot, Bitterroot, and Flathead rivers were important in diluting nutrient concentrations in the Clark Fork. Overall, soluble phosphorus loading to the Clark Fork originated about equally from tributary inflows and wastewater discharges. About two-thirds of the soluble nitrogen loading came from tributaries, with effluents contributing the remaining third. During the summer low flow period, an even greater share of the soluble nutrient loading came from effluents. Tributary sources of soluble nutrient loading were dominated by the Flathead, Bitterroot, and Blackfoot rivers. Gold and Flint creeks ranked fourth in importance as tributary sources of soluble phosphorus and nitrogen, respectively. The Missoula, Butte, and Deer Lodge municipal wastewater treatment plants and the Stone Container Corporation kraft mill discharged most of the soluble nutrient loading from effluents. However, the Warm Springs treatment ponds on Silver Bow Creek removed most of the Butte nutrient load prior to reaching the Clark Fork River

The Right Stuff: biodegradable alternatives to plastic mulch

This project was a continuation of work started by Mark Ingman after he was bothered by the amount of plastic sheeting used in agriculture in China. He was determined to develop an alternative to this environmentally destructive use of plastic by farmers. The plastic “mulch” is used to prevent weeds and maintain soil moisture, but after a season of use the plastic is either thrown into a landfill or burned. Needless to say this creates a lot of unnecessary waste. Mark came back to the United States and put together a team to develop an alternative to the plastic mulch using wool and flax shive waste. The shive waste is an agricultural byproduct. It is composed of biomass available after processing the plant for fibers, oil and seeds. The project was sponsored by the EPA and given a P3 Phase 2 Grant after all initial work. Dr. Skip Rochefort was asked to consult to help develop the product further. He suggested using sodium alginate, a common food additive, to bind the flax shive into a sheet form. Sodium alginate is biodegradable and fit within the sustainability needs of the project. I was tasked with taking Dr. Rochefort's idea and turning it into the final mulch sheet product. The goal of the project was to make this sheet retain moisture and prevent weeds like other mulches but biodegrade over time. At the end of the season, this product would be tilled into the soil as a soil amendment

Evolutionary History of Hunter-Gatherer Marriage Practices

Background: The universality of marriage in human societies around the world suggests a deep evolutionary history of institutionalized pair-bonding that stems back at least to early modern humans. However, marriage practices vary considerably from culture to culture, ranging from strict prescriptions and arranged marriages in some societies to mostly unregulated courtship in others, presence to absence of brideservice and brideprice, and polyandrous to polygynous unions. The ancestral state of early human marriage is not well known given the lack of conclusive archaeological evidence. Methodology: Comparative phylogenetic analyses using data from contemporary hunter-gatherers around the world may allow for the reconstruction of ancestral human cultural traits. We attempt to reconstruct ancestral marriage practices using hunter-gatherer phylogenies based on mitochondrial DNA sequences. Results: Arranged marriages are inferred to go back at least to first modern human migrations out of Africa. Reconstructions are equivocal on whether or not earlier human marriages were arranged because several African hunter-gatherers have courtship marriages. Phylogenetic reconstructions suggest that marriages in early ancestral human societies probably had low levels of polygyny (low reproductive skew) and reciprocal exchanges between the families of marital partners (i.e., brideservice or brideprice). Discussion: Phylogenetic results suggest a deep history of regulated exchange of mates and resources among lineages tha

Pubertal high fat diet: effects on mammary cancer development

INTRODUCTION: Epidemiological studies linking dietary fat intake and obesity to breast cancer risk have produced inconsistent results. This may be due to the difficulty of dissociating fat intake from obesity, and/or the lack of defined periods of exposure in these studies. The pubertal mammary gland is highly sensitive to cancer-causing agents. We assessed how high fat diet (HFD) affects inflammation, proliferative, and developmental events in the pubertal gland, since dysregulation of these can promote mammary tumorigenesis. To test the effect of HFD initiated during puberty on tumorigenesis, we utilized BALB/c mice, for which HFD neither induces obesity nor metabolic syndrome, allowing dissociation of HFD effects from other conditions associated with HFD. METHODS: Pubertal BALB/c mice were fed a low fat diet (12% kcal fat) or a HFD (60% kcal fat), and subjected to carcinogen 7,12-dimethylbenz[a]anthracene (DMBA)-induced tumorigenesis. RESULTS: HFD elevated mammary gland expression of inflammatory and growth factor genes at 3 and 4 weeks of diet. Receptor activator of nuclear factor kappa-B ligand (RANKL), robustly induced at 4 weeks, has direct mitogenic activity in mammary epithelial cells and, as a potent inducer of NF-κB activity, may induce inflammatory genes. Three weeks of HFD induced a transient influx of eosinophils into the mammary gland, consistent with elevated inflammatory factors. At 10 weeks, prior to the appearance of palpable tumors, there were increased numbers of abnormal mammary epithelial lesions, enhanced cellular proliferation, increased growth factors, chemokines associated with immune-suppressive regulatory T cells, increased vascularization, and elevated M2 macrophages. HFD dramatically reduced tumor latency. Early developing tumors were more proliferative and were associated with increased levels of tumor-related growth factors, including increased plasma levels of HGF in tumor-bearing animals. Early HFD tumors also had increased vascularization, and more intra-tumor and stromal M2 macrophages. CONCLUSIONS: Taken together in this non-obesogenic context, HFD promotion of inflammatory processes, as well as local and systemically increased growth factor expression, are likely responsible for the enhanced tumorigenesis. It is noteworthy that although DMBA mutagenesis is virtually random in its targeting of genes in tumorigenesis, the short latency tumors arising in animals on HFD showed a unique gene expression profile, highlighting the potent overarching influence of HFD

CCL2-Mediated Stromal Interactions Drive Macrophage Polarization to Increase Breast Tumorigenesis

CCL2 is an inflammatory cytokine that regulates macrophage activity and is implicated in increased mammographic density and early breast tumorigenesis. The role of CCL2 in mediating stromal interactions that contribute to breast tumorigenesis has yet to be fully elucidated. THP-1-derived macrophages and mammary fibroblasts were co-cultured for 72 h. Fibroblasts and macrophages were analysed for phenotype, expression of inflammatory and ECM-regulatory genes and collagen production. Mice overexpressing CCL2 in the mammary glands were analysed for global gene expression by RNAseq at 12 weeks of age. These mice were cross-bred with PyMT mammary tumour mice to examine the role of CCL2 in tumorigenesis. The co-culture of macrophages with fibroblasts resulted in macrophage polarization towards an M2 phenotype, and upregulated expression of CCL2 and other genes associated with inflammation and ECM remodelling. CCL2 increased the production of insoluble collagen by fibroblasts. A global gene expression analysis of CCL2 overexpressing mice revealed that CCL2 upregulates cancer-associated gene pathways and downregulates fatty acid metabolism gene pathways. In the PyMT mammary tumour model, CCL2 overexpressing mice exhibited increased macrophage infiltration and early tumorigenesis. Interactions between macrophages and fibroblasts regulated by CCL2 can promote an environment that may increase breast cancer risk, leading to enhanced early tumorigenesis

Matrix metalloproteinases and tissue inhibitors of metalloproteinases in gingival crevicular fluid during orthodontic tooth movement.

Contains fulltext : 79854.pdf (publisher's version ) (Closed access)Orthodontic tooth movement requires extensive re-modelling of the periodontium. Matrix metalloproteinases (MMPs) degrade the extracellular matrix during re-modelling, while their activity is regulated by the tissue inhibitors of metalloproteinases (TIMPs). The aim of this study was to investigate differences in MMP and TIMP levels in the gingival crevicular fluid (GCF) at the resorption and apposition sides of orthodontically moved teeth, and to compare these with control teeth. GCF samples were collected from eight orthodontic patients wearing fixed appliances with superelastic nickel-titanium coil springs. The samples were analysed by gelatin zymography, which allows detection of both active and latent MMPs, and reverse zymography for analysis of TIMPs. Western blotting was performed to confirm the identity of MMPs. The data were analysed using either the one-way analysis of variance or the Kruskal-Wallis test. In general, higher levels of MMPs and TIMPs were found at both the resorption and apposition sides compared with the control teeth. Remarkably, partially active MMP-1 was found in GCF from both the resorption and the apposition side but was barely present at the control teeth. TIMP-1 was strongly increased at the apposition side. Gelatinases were mainly present at the resorption side, while gelatinolytic fragments were exclusively detected at the apposition side. MMP-9, which is known to be involved in bone degradation, and a 48 kDa gelatinase were increased at the resorption side. The small increase in TIMP-1 at the resorption side might stimulate bone resorption, whereas the large increase at the apposition side reduces bone resorption. The analysis of MMPs and TIMPs may contribute to the improvement of orthodontic treatment regimens