Regional differences in store-operated Ca2+ entry in the epithelium of the intact human lens

An elevated level of Ca2+ is an important factor in cataract, yet precisely how Ca2+ enters the lens is unknown. Lens epithelial cells contain a range of G-protein–coupled receptors and receptor tyrosine kinases that induce increases in intracellular Ca2+. Receptor-associated Ca2+ influx is, therefore, likely to be an important route for Ca2+ influx to the lens. The authors investigated stimulated and passive Ca2+ influx in in situ human lens epithelium. Ca2+ changes in equatorial (E) and central anterior (CA) epithelial cells were monitored with the use of a Ca2+ indicator (Fluo4) and confocal microscopy. Gene expression was monitored by RT-PCR and immunoblotting. Adenosine triphosphate (ATP) induced Ca2+ responses that were smaller in CA than E. Ca2+ store depletion, using ATP (100 µM) or thapsigargin (1 µM), revealed greater relative store capacity and Ca2+ influx in E. Ca2+ influx was blocked by La3+ (0.5 µM) in both regions. Unstimulated Ca2+ influx was greater in E than CA. Greater expression of Orai1 and STIM1 was detected in E than in CA. Greater Ca2+ store capacity and Ca2+ influx in E compared with CA reflects underlying differences in proliferation and differentiation between the regions. The relatively small resting Ca2+ influx in CA epithelium suggests that store-operated Ca2+ entry (SOCE) is the main route of Ca2+ influx in these cells. Greater resting influx and SOCE in E cells suggests that these are a major route for Ca2+ influx into the lens. Increased expression of Orai1 and STIM1 in E could account for the differences in Ca2+ entry. Receptor activation will modulate Ca2+ influx, and inappropriate activity may contribute to cortical cataract

The Next Generation Space Telescope

In Space Science in the Twenty-First Century, the Space Science Board of the National Research Council identified high-resolution-interferometry and high-throughput instruments as the imperative new initiatives for NASA in astronomy for the two decades spanning 1995 to 2015. In the optical range, the study recommended an 8 to 16-meter space telescope, destined to be the successor of the Hubble Space Telescope (HST), and to complement the ground-based 8 to 10-meter-class telescopes presently under construction. It might seem too early to start planning for a successor to HST. In fact, we are late. The lead time for such major missions is typically 25 years, and HST has been in the making even longer with its inception dating back to the early 1960s. The maturity of space technology and a more substantial technological base may lead to a shorter time scale for the development of the Next Generation Space Telescope (NGST). Optimistically, one could therefore anticipate that NGST be flown as early as 2010. On the other hand, the planned lifetime of HST is 15 years. So, even under the best circumstances, there will be a five year gap between the end of HST and the start of NGST. The purpose of this first workshop dedicated to NGST was to survey its scientific potential and technical challenges. The three-day meeting brought together 130 astronomers and engineers from government, industry and universities. Participants explored the technologies needed for building and operating the observatory, reviewed the current status and future prospects for astronomical instrumentation, and discussed the launch and space support capabilities likely to be available in the next decade. To focus discussion, the invited speakers were asked to base their presentations on two nominal concepts, a 10-meter telescope in space in high earth orbit, and a 16-meter telescope on the moon. The workshop closed with a panel discussion focused mainly on the scientific case, siting, and the programmatic approach needed to bring NGST into being. The essential points of this panel discussion have been incorporated into a series of recommendations that represent the conclusions of the workshop. Speakers were asked to provide manuscripts of their presentation. Those received were reproduced here with only minor editorial changes. The few missing papers have been replaced by the presentation viewgraphs. The discussion that follows each speaker's paper was derived from the question and answer sheets, or if unavailable, from the tapes of the meeting. In the latter case, the editors have made every effort to faithfully represent the discussion

A method for estimating the turbulent kinetic energy dissipation rate from a vertically pointing Doppler lidar, and independent evaluation from balloon-borne in situ measurements

A method of estimating dissipation rates from a vertically pointing Doppler lidar with high temporal and spatial resolution has been evaluated by comparison with independent measurements derived from a balloon-borne sonic anemometer. This method utilizes the variance of the mean Doppler velocity from a number of sequential samples and requires an estimate of the horizontal wind speed. The noise contribution to the variance can be estimated from the observed signal-to-noise ratio and removed where appropriate. The relative size of the noise variance to the observed variance provides a measure of the confidence in the retrieval. Comparison with in situ dissipation rates derived from the balloon-borne sonic anemometer reveal that this particular Doppler lidar is capable of retrieving dissipation rates over a range of at least three orders of magnitude. This method is most suitable for retrieval of dissipation rates within the convective well-mixed boundary layer where the scales of motion that the Doppler lidar probes remain well within the inertial subrange. Caution must be applied when estimating dissipation rates in more quiescent conditions. For the particular Doppler lidar described here, the selection of suitably short integration times will permit this method to be applicable in such situations but at the expense of accuracy in the Doppler velocity estimates. The two case studies presented here suggest that, with profiles every 4 s, reliable estimates of ϵ can be derived to within at least an order of magnitude throughout almost all of the lowest 2 km and, in the convective boundary layer, to within 50%. Increasing the integration time for individual profiles to 30 s can improve the accuracy substantially but potentially confines retrievals to within the convective boundary layer. Therefore, optimization of certain instrument parameters may be required for specific implementations

The DEEP Groth Strip Galaxy Redshift Survey. III. Redshift Catalog and Properties of Galaxies

The Deep Extragalactic Evolutionary Probe (DEEP) is a series of spectroscopic surveys of faint galaxies, targeted at the properties and clustering of galaxies at redshifts z ~ 1. We present the redshift catalog of the DEEP 1 GSS pilot phase of this project, a Keck/LRIS survey in the HST/WFPC2 Groth Survey Strip. The redshift catalog and data, including reduced spectra, are publicly available through a Web-accessible database. The catalog contains 658 secure galaxy redshifts with a median z=0.65, and shows large-scale structure walls to z = 1. We find a bimodal distribution in the galaxy color-magnitude diagram which persists to z = 1. A similar color division has been seen locally by the SDSS and to z ~ 1 by COMBO-17. For red galaxies, we find a reddening of only 0.11 mag from z ~ 0.8 to now, about half the color evolution measured by COMBO-17. We measure structural properties of the galaxies from the HST imaging, and find that the color division corresponds generally to a structural division. Most red galaxies, ~ 75%, are centrally concentrated, with a red bulge or spheroid, while blue galaxies usually have exponential profiles. However, there are two subclasses of red galaxies that are not bulge-dominated: edge-on disks and a second category which we term diffuse red galaxies (DIFRGs). The distant edge-on disks are similar in appearance and frequency to those at low redshift, but analogs of DIFRGs are rare among local red galaxies. DIFRGs have significant emission lines, indicating that they are reddened mainly by dust rather than age. The DIFRGs in our sample are all at z>0.64, suggesting that DIFRGs are more prevalent at high redshifts; they may be related to the dusty or irregular extremely red objects (EROs) beyond z>1.2 that have been found in deep K-selected surveys. (abridged)Comment: ApJ in press. 24 pages, 17 figures (12 color). The DEEP public database is available at http://saci.ucolick.org

Observations of ice multiplication in a weakly convective cell embedded in supercooled mid-level stratus

Simultaneous observations of cloud microphysical properties were obtained by in-situ aircraft measurements and ground based Radar/Lidar. Widespread mid-level stratus cloud was present below a temperature inversion (~5 °C magnitude) at 3.6 km altitude. Localised convection (peak updraft 1.5 m s−1) was observed 20 km west of the Radar station. This was associated with convergence at 2.5 km altitude. The convection was unable to penetrate the inversion capping the mid-level stratus. The mid-level stratus cloud was vertically thin (~400 m), horizontally extensive (covering 100 s of km) and persisted for more than 24 h. The cloud consisted of supercooled water droplets and small concentrations of large (~1 mm) stellar/plate like ice which slowly precipitated out. This ice was nucleated at temperatures greater than −12.2 °C and less than −10.0 °C, (cloud top and cloud base temperatures, respectively). No ice seeding from above the cloud layer was observed. This ice was formed by primary nucleation, either through the entrainment of efficient ice nuclei from above/below cloud, or by the slow stochastic activation of immersion freezing ice nuclei contained within the supercooled drops. Above cloud top significant concentrations of sub-micron aerosol were observed and consisted of a mixture of sulphate and carbonaceous material, a potential source of ice nuclei. Particle number concentrations (in the size range 0.1<D<3.0 μm) were measured above and below cloud in concentrations of ~25 cm−3. Ice crystal concentrations in the cloud were constant at around 0.2 L−1. It is estimated that entrainment of aerosol particles into cloud cannot replenish the loss of ice nuclei from the cloud layer via precipitation. Precipitation from the mid-level stratus evaporated before reaching the surface, whereas rates of up to 1 mm h−1 were observed below the convective feature. There is strong evidence for the Hallett-Mossop (HM) process of secondary ice particle production leading to the formation of the precipitation observed. This includes (1) Ice concentrations in the convective feature were more than an order of magnitude greater than the concentration of primary ice in the overlaying stratus, (2) Large concentrations of small pristine columns were observed at the ~−5 °C level together with liquid water droplets and a few rimed ice particles, (3) Columns were larger and increasingly rimed at colder temperatures. Calculated ice splinter production rates are consistent with observed concentrations if the condition that only droplets greater than 24 μm are capable of generating secondary ice splinters is relaxed. This case demonstrates the importance of understanding the formation of ice at slightly supercooled temperatures, as it can lead to secondary ice production and the formation of precipitation in clouds which may not otherwise be considered as significant precipitation sources

z~7 galaxy candidates from NICMOS observations over the HDF South and the CDF-S and HDF-N GOODS fields

We use ~88 arcmin**2 of deep (>~26.5 mag at 5 sigma) NICMOS data over the two GOODS fields and the HDF South to conduct a search for bright z>~7 galaxy candidates. This search takes advantage of an efficient preselection over 58 arcmin**2 of NICMOS H-band data where only plausible z>~7 candidates are followed up with NICMOS J-band observations. ~248 arcmin**2 of deep ground-based near-infrared data (>~25.5 mag, 5 sigma) is also considered in the search. In total, we report 15 z-dropout candidates over this area -- 7 of which are new to these search fields. Two possible z~9 J-dropout candidates are also found, but seem unlikely to correspond to z~9 galaxies. The present z~9 search is used to set upper limits on the prevalence of such sources. Rigorous testing is undertaken to establish the level of contamination of our selections by photometric scatter, low mass stars, supernovae (SNe), and spurious sources. The estimated contamination rate of our z~7 selection is ~24%. Through careful simulations, the effective volume available to our z>~7 selections is estimated and used to establish constraints on the volume density of luminous (L*(z=3), or -21 mag) galaxies from these searches. We find that the volume density of luminous star-forming galaxies at z~7 is 13_{-5}^{+8}x lower than at z~4 and >25x lower (1 sigma) at z~9 than at z~4. This is the most stringent constraint yet available on the volume density of >~L* galaxies at z~9. The present wide-area, multi-field search limits cosmic variance to <20%. The evolution we find at the bright end of the UV LF is similar to that found from recent Subaru Suprime-Cam, HAWK-I or ERS WFC3/IR searches. The present paper also includes a complete summary of our final z~7 z-dropout sample (18 candidates) identified from all NICMOS observations to date (over the two GOODS fields, the HUDF, galaxy clusters).Comment: 13 pages, 6 figures, 6 tables, accepted for publication in the Astrophysical Journal, replaced to match accepted version, see http://firstgalaxies.org/astronomers-area/ for a link to a complete reduction of the NICMOS observations over the two GOODS field

The DEEP Groth Strip Survey IX: Evolution of the Fundamental Plane of Field Galaxies

Fundamental Plane studies provide an excellent means of understanding the evolutionary history of early-type galaxies. Using the Low Resolution Imaging Spectrograph on the Keck telescope, we obtained internal stellar kinematic information for 36 field galaxies in the Groth Strip--21 early-type and 15 disk galaxies. Their redshifts range from 0.3--1.0, with a median redshift 0.8. The slope of the relation shows no difference compared with the local slope. However, there is significant evolution in the zero-point offset; an offset due to evolution in magnitude requires a 2.4 magnitude luminosity brightening at z=1. We see little differences of the offset with bulge fraction, which is a good surrogate for galaxy type. Correcting for the luminosity evolution reduces the orthogonal scatter in the Fundamental Plane to 8%, consistent with the local scatter. This scatter is measured for our sample, and does not include results from other studies which may have different selection effects. The difference in the degree of evolution between our field sample and published cluster galaxies suggests a more recent formation epoch--around z=1.5 for field galaxies compared to z>2.0 for cluster galaxies. The magnitude difference implies that the field early-type galaxies are about 2 Gyr younger than the cluster ellipticals using standard single-burst models. However, the same models imply a significant change in the rest-frame U-B color from then to present, which is not seen in our sample. Continuous low-level star formation, however, would serve to explain the constant colors over this large magnitude change. A consistent model has 7% of the stellar mass created after the initial burst, using an exponentially decaying star formation rate with an e-folding time of 5 Gyr.Comment: 25 pages, accepted for publication in the Astrophysical Journal, high resolution version at http://hoku.as.utexas.edu/~gebhardt/FPpaper.p

The DEEP Groth Strip Survey II. Hubble Space Telescope Structural Parameters of Galaxies in the Groth Strip

The quantitative morphological classification of distant galaxies is essential to the understanding of the evolution of galaxies over the history of the Universe. This paper presents Hubble Space Telescope WFPC2 F606W and F814W photometric structural parameters for 7450 galaxies in the ``Groth Strip.'' These parameters are based on a two-dimensional bulge+disk surface brightness model and were obtained using an automated reduction and analysis pipeline described in detail here. A first set of fits was performed separately in each bandpass, and a second set of fits was performed simultaneously on both bandpasses. The information produced by these two types of fits can be used to explore different science goals. Systematic and random fitting errors in all structural parameters as well as bulge and disk colors are carefully characterized through extensive sets of simulations. The results of these simulations are given in catalogs similar to the real science catalogs so that both real and simulated measurements can be sampled according to the same selection criteria to show biases and errors in the science data subset of interest. The effects of asymmetric structures on the recovered bulge+disk fitting parameters are also explored through simulations. The full multidimensional photometric survey selection function of the Groth Strip is also computed. This selection function, coupled to bias maps from simulations, provides a complete and objective reproduction of the observational limits, and these limits can be applied to theoretical predictions from galaxy evolution models for direct comparisons with the data.Comment: 22 pages+16 PNG/JPEG figures, ApJ Supplements accepted. Full resolution paper+figures at http://deep.ucolick.org/publications.html . Tables 2 and 3 available upon publication of the redshift catalogue (Phillips et al. in prep.). Observed quantities available through interactive database at http://saci.ucolick.or

The DEEP Groth Strip Survey. I. The Sample

The Deep Extragalactic Exploratory Probe (DEEP) is a multi-phase research program dedicated to the study of the formation and evolution of galaxies and of large scale structure in the distant Universe. This paper describes the first five-year phase, denoted DEEP1. A series of ten DEEP1 papers will discuss a range of scientific topics (e.g., the study of photometric and spectral properties of a general distant galaxy survey, the evolution observed in galaxy populations of varied morphologies). The observational basis for these studies is the Groth Survey Strip field, a 127 square arcminute region which has been observed with the Hubble Space Telescope in both broad I-band and V-band optical filters and with the Low Resolution Imaging Spectrograph on the Keck Telescopes. Catalogs of photometric and structural parameters have been constructed for 11,547 galaxies and stars at magnitudes brighter than 29, and spectroscopy has been conducted for a magnitude-color weighted subsample of 818 objects. We evaluate three independent techniques for constructing an imaging catalog for the field from the HST data, and discuss the depth and sampling of the resultant catalogs. The selection of the spectroscopic subsample is discussed, and we describe the multifaceted approach taken to prioritizing objects of interest for a variety of scientific subprograms. A series of Monte Carlo simulations then demonstrates that the spectroscopic subsample can be adequately modeled as a simple function of magnitude and color cuts in the imaging catalog.Comment: ApJS accepted, 15 pages, 12 figures. Version with higher-quality figures available at http://astronomy.nmsu.edu/nicol

FRA2A is a CGG repeat expansion associated with silencing of AFF3

Folate-sensitive fragile sites (FSFS) are a rare cytogenetically visible subset of dynamic mutations. Of the eight molecularly characterized FSFS, four are associated with intellectual disability (ID). Cytogenetic expression results from CGG tri-nucleotide-repeat expansion mutation associated with local CpG hypermethylation and transcriptional silencing. The best studied is the FRAXA site in the FMR1 gene, where large expansions cause fragile X syndrome, the most common inherited ID syndrome. Here we studied three families with FRA2A expression at 2q11 associated with a wide spectrum of neurodevelopmental phenotypes. We identified a polymorphic CGG repeat in a conserved, brain-active alternative promoter of the AFF3 gene, an autosomal homolog of the X-linked AFF2/FMR2 gene: Expansion of the AFF2 CGG repeat causes FRAXE ID. We found that FRA2A-expressing individuals have mosaic expansions of the AFF3 CGG repeat in the range of several hundred repeat units. Moreover, bisulfite sequencing and pyrosequencing both suggest AFF3 promoter hypermethylation. cSNP-analysis demonstrates monoallelic expression of the AFF3 gene in FRA2A carriers thus predicting that FRA2A expression results in functional haploinsufficiency for AFF3 at least in a subset of tissues. By whole-mount in situ hybridization the mouse AFF3 ortholog shows strong regional expression in the developing brain, somites and limb buds in 9.5-12.5dpc mouse embryos. Our data suggest that there may be an association between FRA2A and a delay in the acquisition of motor and language skills in the families studied here. However, additional cases are required to firmly establish a causal relationship