Analysis of Fusarium-Common Beans Pathosystem in Aguascalientes, Mexico

In Mexico, high incidences of Fusarium affect common bean (Phaseolus vulgaris L.) production, reducing grain yields due to seedling death and crop standing reductions. Production of resistant germplasm could be an appropriate strategy for grain yield increasing. Bean breeding programs need the former analysis of plant-pathogen pathosystem to perform the selection of segregating populations with improved resistance to root rot pathogens and the best agroecosystem adaptation. Here, we report our results on characterization of genetic variability patterns of Fusarium solani f. sp. phaseoli (FSP) from Aguascalientes, México; the analysis of P. vulgaris germplasm reactions to highly and naturally FSP-infested field and controlled conditions; and the identification of genetic basis of resistance to FSP root rot in segregating common bean populations. Significant genetic variability in FSP isolates from Aguascalientes and other regions of México was found. Also, we found high variation on reactions to FSP root rots, resistance was more frequent on black seed-coated beans, and susceptibility was common in pinto beans. Resistance to FSP in BAT 477 seedlings was associated with one quantitative trait loci (QTL)

RITEC & CRIS: Interoperabilidad para visibilidad y medición del impacto de la producción científica energética

[ES]El Tecnológico de Monterrey cuenta con dos plataformas para la gestión y la difusión del conocimiento científico, las cuales son el Sistema de Información de Investigación Actual (Current Research Information System – CRIS) para la gestión institucional interna y el Repositorio Institucional (RI) desarrollado como parte del movimiento educativo abierto para dar visibilidad externa en formato abierto a la investigación desarrollada por la institución. Los investigadores capturan su producción científica, como son artículos de revista, libros, capítulos de libro, artículos de conferencias, tesis entre otros, en los dos sistemas y esto hace que la captura de la misma información se duplique. El objetivo de este capítulo es dar a conocer la estrategia que se llevó a cabo, para encontrar puntos en común de ambos sistemas y así permitir la interoperabilidad entre ellos, a través de la iniciativa del proyecto “Laboratorio Binacional para la Gestión Inteligente de la Sustentabilidad Energética y la Formación Tecnológica”. Para abordar el tema se planteó la siguiente pregunta de investigación, ¿cómo se relacionan los CRIS y los del RI para que sean interoperables? Para contestar a ello, se utilizó el método comparativo. Los resultados apuntan a que su relación se entrelaza al homogeneizar los metadatos utilizados en el CRIS y en el RI a través de protocolos de interoperabilidad (e.g. SWORD, OAI) y enviar de forma unidireccional la captura de la producción científica del CRIS hacia el RI, en primera instancia, para la captura de la producción científica del proyecto mencionado, a través de una interfaz única

Bean rust resistance and yield of black bean genotypes under field conditions

Objective: To identify black bean lines resistant to rust under field conditions, and with yield equal to or greater than that of three varieties for commercial use in Veracruz. Design/methodology/approach: The genotypes were evaluated in a randomized block experimental design with three replications. Its reaction to rust was rated on an incidence scale of 1 to 9 and grain yield was estimated in kilograms per hectare. Analyses of variance were performed and to separate averages, the Least Significant Difference was applied at 0.05. Correlation analyses were also carried out between the rust incidence values ​​and the yield of the genotypes at each evaluation site. Results: Nine lines were resistant to rust with an average incidence of between 1.78 and 3.33, of which Jamapa Plus/XRAV-187-3-4-4 was the most productive with an average yield of 2183.4 kg ha-1, statistically similar. to that obtained by the Jamapa Plus/XRAV-187-3-4-1 and Jamapa Plus/XRAV-187-3-1-2 lines and the Verdín variety, and significantly higher than that of the rest of the genotypes. Limitations on study/implications: Although the rust occurred in the three study locations, its degree of incidence and the stage in which it appeared were different. However, in all cases, the degree of infection was sufficient to evaluate the reaction of the genotypes to this disease. Findings/conclusions: Three lines resistant to rust were identified under field conditions, with an average yield significantly higher than that of the Negro Jamapa and Negro Medellín varieties.  Objective: To identify black beans lines resistant to bean rust under field conditions, which have similar or higher yield than three commercial varieties sown in Veracruz, Mexico. Design/Methodology/Approach: The genotypes were evaluated using a completely randomized block design. The reaction of the genotypes to bean rust was qualified using a 1-9 incidence rate, while grain yield was estimated in kg ha-1. An analysis of variance was carried out and the resulting means were divided using a 0.05 Least Significant Difference (LSD). Additionally, a correlation analysis between the incidence values of bean rust and the yield of the genotypes was carried out in each evaluation site. Results: Nine lines were resistant to bean rust (incidence average: 1.78-3.33). Out of these lines, Jamapa Plus/XRAV-187-3-4-4 was the most productive line, with a 2,183.4 kg ha-1 average yield. This result is statistically similar to the results of the Jamapa Plus/XRAV-187-3-4-1 and Jamapa Plus/XRAV-187-3-1-2 lines and the Verdín variety. In addition, the results of Jamapa Plus/XRAV-187-3-4-4 are slightly higher than the results obtained by the rest of the genotypes. Study Limitations/Implications: Although bean rust was recorded in three of the localities of the study area, the incidence degree and the stage when it was found were different. However, in all the cases, the infection degree was enough to evaluate the reactions of the genotypes to this disease. Findings/Conclusions: Three bean rust-resistant lines were identified under field conditions. They had a significantly higher average yield than the Negro Jamapa and Negro Medellín varieties

Dry selection and wet evaluation for the rational discovery of new anthelmintics

Helminths infections remain a major problem in medical and public health. In this report, atom-based 2D bilinear indices, a TOMOCOMD-CARDD (QuBiLs-MAS module) molecular descriptor family and linear discriminant analysis (LDA) were used to find models that differentiate among anthelmintic and non-anthelmintic compounds. Two classification models obtained by using non-stochastic and stochastic 2D bilinear indices, classified correctly 86.64% and 84.66%, respectively, in the training set. Equation 1(2) correctly classified 141(135) out of 165 [85.45%(81.82%)] compounds in external validation set. Another LDA models were performed in order to get the most likely mechanism of action of anthelmintics. The model shows an accuracy of 86.84% in the training set and 94.44% in the external prediction set. Finally, we carry out an experiment to predict the biological profile of our ‘in-house’ collections of indole, indazole, quinoxaline and cinnoline derivatives (∼200 compounds). Subsequently, we selected a group of nine of the theoretically most active structures. Then, these chemicals were tested in an invitro assay and one good candidate (VA5-5c) as fasciolicide compound (100% of reduction at concentrations of 50 and 10 mg/L) was discovered.Yovani Marrero-Ponce acknowledges the support from USFQ with partial finance of project ID5455Peer Reviewe

Identification of black bean (Phaseolus vulgaris L.) genotypes resistant to anthracnose and rust for Veracruz and Chiapas, Mexico

Objective:to determine the reaction of 53 lines and three varieties of black beans to inoculation with Uromyces appendiculatusand Colletotrichum lindemuthianum, to identify genotypes resistant to rust and anthracnose. Design/methodology/approach:ten seedlings of each genotype were inoculated in the greenhouse with a suspension of U. appendiculatusurediospores and another 10 with a suspension of C. lindemuthianumconidia. Fourteen days after inoculation, the reaction of the genotypes to rust was evaluated with a severity scale of 1 to 6, and to anthracnose, with a scale of 0 to 4. The data were analyzed in a completely randomized design with 10 replications per treatment and for the separation of averages the LSD at 0.05 was applied. Results:41 genotypes showed a hypersensitivity reaction to rust, of which 25 had a reaction value of 2.0, statistically lower than that of the controls. In turn, 45 genotypes were resistant to anthracnose, of which 18 had a value of 1.0, statistically similar to that of Negro Jamapa and lower than that of the rest of the genotypes. Limitations of the study/implications:due to the diversity of races of both pathogens, the genotypes were inoculated with monopustular isolates of the main races of U. appendiculatusand with monosporic cultures of C. lindemuthianum, which occur in the bean crops of Veracruz and Chiapas. Findings/conclusions:25 lines resistant to rust and 18 to anthracnose were identified, which stood out for presenting the least damage from these diseases.Objective: to determine the reaction of 53 lines and three varieties of black bean (Phaseolus vulgaris L.) to inoculation with Uromyces appendiculatus and Colletotrichum lindemuthianum, to identify genotypes resistant to rust and anthracnose.Design/methodology/approach: 10 seedlings of each genotype were inoculated in the greenhouse with a suspension of U. appendiculatus uredospores and another 10 with a suspension of C. lindemuthianum conidia. At 14 days after inoculation, the reaction of the genotypes to rust was evaluated with a severity scale of 1 to 6, and to anthracnose, with ascale of 0 to 4. The data were analyzed in a completely randomized design with 10 replications per treatment and LSD at 0.05 was applied for the separation of averages.Results: 41 genotypes showed a hypersensitivity reaction to rust, of which 25 had a reaction value of 2.0, statistically lower than those of controls. In turn, 45 genotypes were resistant to anthracnose, of which 18 had a value of 1.0, statistically similar to that of Negro Jamapa and lower than those of the rest of the genotypes.Study limitations/implications: due to the diversity of races of both pathogens, the genotypes were inoculated with monopustular isolates of the principal races of U. appendiculatus and with monosporic cultures of C. lindemuthianum, which occur in the bean crops of Veracruz and Chiapas.Findings/conclusions: 25 lines resistant to rust and 18 to anthracnose were identified, which stood out for presenting the least damage from these diseases

Nuclear Activity In Isolated Galaxies

We present a spectroscopic study of the incidence of AGN nuclear activity in two samples of isolated galaxies (Karachentseva, V.E. & Varela, J.). Our results show that the incidence of non-thermal nuclear activity is about 43% and 31% for galaxies with emission lines and for the total sample 40% and 27% respectively. For the first time we have a large number of bona-fide isolated galaxies (513 objects), with statistically significant number of all types. We find a clear relation between bulge mass and the incidence of nuclear activity in the sample with emission lines. This relation becomes flatter when we take into account the complete sample with no emission line galaxies. A large fraction (~70%) of elliptical galaxies or early type spirals have an active galactic nucleus and ~70% of them are LINERs. Only 3% of the AGN show the presence of broad lines (a not a single one can be classified as type 1 AGN). This is a remarkable result which is completely at odds with the unified model even if we consider warped or clumpy tori. Finally, we interpret the large fraction of AGN in isolated galaxies as the result of secular evolution of their supermasive black holes.Comment: 7 figures, 7 table

Liquid biopsy-based protein biomarkers for risk prediction, early diagnosis, and prognostication of cholangiocarcinoma

Cholangiocarcinoma (CCA), heterogeneous biliary tumours with dismal prognosis, lacks accurate early diagnostic methods especially important for individuals at high-risk (i.e. those with primary sclerosing cholangitis [PSC]). Here, we searched for protein biomarkers in serum extracellular vesicles (EVs). EVs from patients with isolated PSC (n = 45), concomitant PSC-CCA (n = 44), PSC who developed CCA during follow-up (PSC to CCA; n = 25), CCAs from non-PSC aetiology (n = 56), and hepatocellular carcinoma (n = 34) and healthy individuals (n = 56) were characterised by mass spectrometry. Diagnostic biomarkers for PSC-CCA, non-PSC CCA, or CCAs regardless of aetiology (Pan-CCAs) were defined and validated by ELISA. Their expression was evaluated in CCA tumours at a single-cell level. Prognostic EV biomarkers for CCA were investigated. High-throughput proteomics of EVs identified diagnostic biomarkers for PSC-CCA, non-PSC CCA, or Pan-CCA, and for the differential diagnosis of intrahepatic CCA and hepatocellular carcinoma, which were cross-validated by ELISA using total serum. Machine learning-based algorithms disclosed CRP/FIBRINOGEN/FRIL for the diagnosis of PSC-CCA (local disease [LD]) vs. isolated PSC (AUC = 0.947; odds ratio [OR] =36.9) and, combined with carbohydrate antigen 19-9, overpowers carbohydrate antigen 19-9 alone. CRP/PIGR/VWF allowed the diagnosis of LD non-PSC CCAs vs. healthy individuals (AUC = 0.992; OR = 387.5). It is noteworthy that CRP/FRIL accurately diagnosed LD Pan-CCA (AUC = 0.941; OR = 89.4). Levels of CRP/FIBRINOGEN/FRIL/PIGR showed predictive capacity for CCA development in PSC before clinical evidence of malignancy. Multi-organ transcriptomic analysis revealed that serum EV biomarkers were mostly expressed in hepatobiliary tissues, and single-cell RNA sequencing and immunofluorescence analysis of CCA tumours showed their presence mainly in malignant cholangiocytes. Multivariable analysis unveiled EV prognostic biomarkers, with COMP/GNAI2/CFAI and ACTN1/MYCT1/PF4V associated negatively and positively with patients' survival, respectively. Serum EVs contain protein biomarkers for the prediction, early diagnosis, and prognostication of CCA that are detectable using total serum, representing a tumour cell-derived liquid biopsy tool for personalised medicine. The accuracy of current imaging tests and circulating tumour biomarkers for cholangiocarcinoma (CCA) diagnosis is far from satisfactory. Most CCAs are considered sporadic, although up to 20% of patients with primary sclerosing cholangitis (PSC) develop CCA during their lifetime, constituting a major cause of PSC-related death. This international study has proposed protein-based and aetiology-related logistic models with predictive, diagnostic, or prognostic capacities by combining two to four circulating protein biomarkers, moving a step forward into personalised medicine. These novel liquid biopsy tools may allow the (i) easy and non-invasive diagnosis of sporadic CCAs, (ii) identification of patients with PSC with higher risk for CCA development, (iii) establishment of cost-effective surveillance programmes for the early detection of CCA in high-risk populations (e.g. PSC), and (iv) prognostic stratification of patients with CCA, which, altogether, may increase the number of cases eligible for potentially curative options or to receive more successful treatments, decreasing CCA-related mortality.Spanish Carlos III Health Institute (ISCIII

Temporal relationship of serum markers and tissue damage during acute intestinal ischemia/reperfusion

OBJECTIVE: It is essential to identify a serological marker of injury in order to study the pathophysiology of intestinal ischemia reperfusion. In this work, we studied the evolution of several serological markers after intestinal ischemia reperfusion injury in rats. The markers of non-specific cell damage were aspartate aminotransferase, alanine aminotransaminase, and lactic dehydrogenase, the markers of inflammation were tumor necrosis factor alpha, interleukin-6, and interleukin-1 beta, and the markers of intestinal mucosal damage were intestinal fatty acid binding protein and D-lactate. We used Chius classification to grade the histopathological damage. METHODS: We studied 35 Wistar rats divided into groups according to reperfusion time. The superior mesenteric artery was clamped for 30 minutes, and blood and biopsies were collected at 1, 3, 6, 12, 24, and 48 hours after reperfusion. We plotted the mean ¡ standard deviation and compared the baseline and maximum values for each marker using Student’s t-test. RESULTS: The maximum values of interleukin-1 beta and lactic dehydrogenase were present before the maximal histopathological damage. The maximum tumor necrosis factor alpha and D-lactate expressions coincided with histopathological damage. Alanine aminotransaminase and aspartate aminotransferase had a maximum expression level that increased following the histopathological damage. The maximum expressions of interluken-6 and intestinal fatty acid binding protein were not significantly different from the Sham treated group. CONCLUSION: For the evaluation of injury secondary to acute intestinal ischemia reperfusion with a 30 minute ischemia period, we recommend performing histopathological grading, quantification of D-lactate, which is synthesized by intestinal bacteria and is considered an indicator of mucosal injury, and quantification of tumor necrosis factor alpha as indicators of acute inflammation three hours after reperfusion

Non-motor symptom burden in patients with Parkinson's disease with impulse control disorders and compulsive behaviours : results from the COPPADIS cohort

The study was aimed at analysing the frequency of impulse control disorders (ICDs) and compulsive behaviours (CBs) in patients with Parkinson's disease (PD) and in control subjects (CS) as well as the relationship between ICDs/CBs and motor, nonmotor features and dopaminergic treatment in PD patients. Data came from COPPADIS-2015, an observational, descriptive, nationwide (Spain) study. We used the validated Questionnaire for Impulsive-Compulsive Disorders in Parkinson's Disease-Rating Scale (QUIP-RS) for ICD/CB screening. The association between demographic data and ICDs/CBs was analyzed in both groups. In PD, this relationship was evaluated using clinical features and treatment-related data. As result, 613 PD patients (mean age 62.47 ± 9.09 years, 59.87% men) and 179 CS (mean age 60.84 ± 8.33 years, 47.48% men) were included. ICDs and CBs were more frequent in PD (ICDs 12.7% vs. 1.6%, p < 0.001; CBs 7.18% vs. 1.67%, p = 0.01). PD patients had more frequent previous ICDs history, premorbid impulsive personality and antidepressant treatment (p < 0.05) compared with CS. In PD, patients with ICDs/CBs presented younger age at disease onset, more frequent history of previous ICDs and premorbid personality (p < 0.05), as well as higher comorbidity with nonmotor symptoms, including depression and poor quality of life. Treatment with dopamine agonists increased the risk of ICDs/CBs, being dose dependent (p < 0.05). As conclusions, ICDs and CBs were more frequent in patients with PD than in CS. More nonmotor symptoms were present in patients with PD who had ICDs/CBs compared with those without. Dopamine agonists have a prominent effect on ICDs/CBs, which could be influenced by dose