NEOGLOBOQUADRINA ATLANTICA PRAEATLANTICA, NEW SUBSPECIESFROM LATE MIDDLE MIOCENE

A new subspecies of Neogloboquadrina atlantica atlantica previously reported under different names, is here distinguished and named Neogloboquadrina atlantica praeatlantica. Its first occurrence into the Mediterranean dated at 11.78 Ma, is not evolutionary appearance but is related to a global climatic cooling which pushed southwards from high latitude this taxon; however, the area of its evolutionary appearance is not clearly defined; its extinction occurs within the Globigerinoides obliquus extremus Zone. N. atlantica atlantica which evolves from N. atlantica praeatlantica occurs in the Mediterranean at 11.15 Ma, just after the Mediterranean exit of Paragloborotalia siakensis (Le Roy).  &nbsp

AN INTEGRATED CALCAREOUS PLANKTON BIOSTRATIGRAPHIC SCHEME AND BIOCHRONOLOGY FOR THE MEDITERRANEAN MIDDLE MIOCENE

The relative position of 30 main bioevents  pertaining to calcareous nannofossils and planktonic foraminifera was identified in the time interval between 13.75 Ma and 10.50 Ma, based on the quantitative study of the those microfossils in three Mediterranean sections spanning the late Langhian – lower Tortonian stratigraphic interval. The events were correlated  to the astronomic target curve using a cyclostratigraphic approach, resulting in a very detailed biostratigraphic and biochronologic subdivision of the interval. The zonal scheme proposed by Fornaciari et al. (1996) was adopted for the calcareous nannofossils, but three subzones were identified in the MMN7 Zone. For the planktonic foraminifera reference is made to the zonal scheme recently proposed by Foresi et al. (1998), slightly modified in order to increase its biostratigraphic resolution. The age of all the zonal boundaries is reported.&nbsp

HIGH RESOLUTION CALCAREOUS PLANKTON BIOSTRATIGRAPHY OF THE SERRAVALLIAN SUCCESSION OF THE TREMITI ISLANDS (ADRIATIC SEA, ITALY)

The planktonic foraminifer and calcareous nannofossil content of two Middle Miocene sections of the Tremiti Islands (Southern Adriatic Sea) have been studied. The two sections are composed of marly limestones rich in calcareous plankton which show cyclic alternations of indurated (higher carbonate content) whitish and less indurated grey or reddish beds. The two sections represent a succession with a total thickness of 38 m. Samples have been collected at a mean spacing of 10-15 cm; qualitative analyses were performed on one sample per meter but quantitative analyses were made for each sample. The abundance fluctuations of several marker species proved to be a very useful tool to correlate the two sections. The astronomical calibration of the sedimentary cycles provided absolute ages for all the recognised calcareous plankton bioevents.&nbsp

ASTROCHRONOLOGICAL CALIBRATION OF THE UPPER SERRAVALLIAN/LOWER TORTONIAN SEDIMENTARY SEQUENCE AT TREMITI ISLANDS(ADRIATIC SEA, SOUTHERN ITALY)

A high resolution cyclostratigraphic study was carried out on a cyclicaly bedded succession of late Middle Miocene deep marine deposits from the Tremiti Islands, Adriatic sea (Italy). Astronomical calibration of the sedimentary cycles provides absolute ages for different calcareous plankton bioevents, widely used for intra Mediterranean correlation, in the interval between 11.12 and 12.60 Ma. The sedimentary record of the S. nicola composite section consists of an alternation of indurated, withish coloured, CaCo3 rich and grey less indurated, CaCo3 poor marly beds, at times replaced by red coloured CaCo3 poor marls. Results of direct correlation between the La 90 (1,1) solution of the insolation curve and the cyclic lithologic patterns occurring in the studied sections, combined with results of spectral methodologies applied on the climate sensitive data (CaCo3 and Globigerinoides) showed that the classic Milankovitch periodicity can be represented through the modulation forcing of the studied sedimentary records.&nbsp

Autologous anti-GD2 CAR T cells efficiently target primary human glioblastoma

Glioblastoma (GBM) remains a deadly tumor. Treatment with chemo-radiotherapy and corticosteroids is known to impair the functionality of lymphocytes, potentially compromising the development of autologous CAR T cell therapies. We here generated pre-clinical investigations of autologous anti-GD2 CAR T cells tested against 2D and 3D models of GBM primary cells. We detected a robust antitumor effect, highlighting the feasibility of developing an autologous anti-GD2 CAR T cell-based therapy for GBM patients

RNA-Based Assay for Next-Generation Sequencing of Clinically Relevant Gene Fusions in Non-Small Cell Lung Cancer

Gene fusions represent novel predictive biomarkers for advanced non-small cell lung cancer (NSCLC). In this study, we validated a narrow NGS gene panel able to cover therapeutically-relevant gene fusions and splicing events in advanced-stage NSCLC patients. To this aim, we first assessed minimal complementary DNA (cDNA) input and the limit of detection (LoD) in different cell lines. Then, to evaluate the feasibility of applying our panel to routine clinical samples, we retrospectively selected archived lung adenocarcinoma histological and cytological (cell blocks) samples. Overall, our SiRe RNA fusion panel was able to detect all fusions and a splicing event harbored in a RNA pool diluted up to 2 ng/µL. It also successfully analyzed 46 (95.8%) out of 48 samples. Among these, 43 (93.5%) out of 46 samples reproduced the same results as those obtained with conventional techniques. Intriguingly, the three discordant results were confirmed by a CE-IVD automated real-time polymerase chain reaction (RT-PCR) analysis (Easy PGX platform, Diatech Pharmacogenetics, Jesi, Italy). Based on these findings, we conclude that our new SiRe RNA fusion panel is a valid and robust tool for the detection of clinically relevant gene fusions and splicing events in advanced NSCLC

An approach to prevent frailty in community dwelling older adults: a pilot study performed in Campania region in the framework of the PERSSILAA project

We developed and tested an innovative physical training method in older adults that embeds the gym program into everyday life in the most conservative way possible. Physical training was included in the activities of local parishes where older women from Southern Italy spend most of their free time and was delivered by trained physical therapists with the support of an ICT tool known as CoCo. 113 older women (aged 72.0 [69.0-75.0] years) noncompliant to conventional exercise programs participated to the study. 57 of them underwent the final anthropometric assessment and 50 the final physical tests. In study completers handgrip strength and physical performance evaluated with the chair-stand, the two minutes step and the chair-sit and -reach tests significantly improved. Quality of life as evaluated with the EuroQol-5dimension (EQ-5D) questionnaire improved as well. In conclusion, a training program designed to minimally impact on life habits of older people is effective in improving fitness in patients noncompliant to other to physical exercise programs

Innovative approaches to active and healthy ageing: Campania experience to improve the adoption of innovative good practices

The demographic projections on the European population predict that people aged over 60 will increase by about two million/year in the next decades. Since 2012, the Campania Reference Site of the European Innovation Partnership on Active and Healthy Ageing supports the innovation of the Regional Health System, to face up demographic changes and sustainability. Campania Reference Site provides the opportunity to connect loco-regional stakeholders in social and health care services (universities, healthcare providers, social services, local communities and municipalities), with international organizations, in order to adopt and scale up innovative solutions and approaches. This paper describes the building process of Campania Reference Site and the main results achieved, that have been allowing it to become a hub for open innovation in the field of active and healthy aging at regional, national and international level

Cabbage and fermented vegetables : From death rate heterogeneity in countries to candidates for mitigation strategies of severe COVID-19

Large differences in COVID-19 death rates exist between countries and between regions of the same country. Some very low death rate countries such as Eastern Asia, Central Europe, or the Balkans have a common feature of eating large quantities of fermented foods. Although biases exist when examining ecological studies, fermented vegetables or cabbage have been associated with low death rates in European countries. SARS-CoV-2 binds to its receptor, the angiotensin-converting enzyme 2 (ACE2). As a result of SARS-CoV-2 binding, ACE2 downregulation enhances the angiotensin II receptor type 1 (AT(1)R) axis associated with oxidative stress. This leads to insulin resistance as well as lung and endothelial damage, two severe outcomes of COVID-19. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is the most potent antioxidant in humans and can block in particular the AT(1)R axis. Cabbage contains precursors of sulforaphane, the most active natural activator of Nrf2. Fermented vegetables contain many lactobacilli, which are also potent Nrf2 activators. Three examples are: kimchi in Korea, westernized foods, and the slum paradox. It is proposed that fermented cabbage is a proof-of-concept of dietary manipulations that may enhance Nrf2-associated antioxidant effects, helpful in mitigating COVID-19 severity.Peer reviewe