Characterization of slow and fast phase nystagmus

A current literature review of the analog and digital process of vestibular and optical kinetic nystagmus reveals little agreement in the methods used by various labs. The strategies for detection of saccade (fast phase velocity component of nystagmus) vary between labs, and most of the process have not been evaluated and validated with a standard database. A survey was made of major vestibular labs in the U.S. that perform computer analyses of vestibular and optokinetic reflexes to stimuli, and a baseline was established from which to standardize data acquisition and analysis programs. The concept of an Error Index was employed as the criterium for evaluating the performance of the vestibular analysis software programs. The performance criterium is based on the detection of saccades and is the average of the percentages of missed detections and false detections. Evaluation of the programs produced results for lateral gaze with saccadic amplitude of one, two, three, five, and ten degrees with various signal-to-noise ratios. In addition, results were obtained for sinusoidal pursuit of 0.05, 0.10, and 0.50 Hz with saccades from one to ten degrees at various signal-to-noise ratios. Selection of the best program was made from the performance in the lateral gaze with three degrees of saccadic amplitude and in the 0.10 Hz sinusoid with three degrees of saccadic amplitude

Conservative and disruptive modes of adolescent change in human brain functional connectivity

Adolescent changes in human brain function are not entirely understood. Here, we used multiecho functional MRI (fMRI) to measure developmental change in functional connectivity (FC) of resting-state oscillations between pairs of 330 cortical regions and 16 subcortical regions in 298 healthy adolescents scanned 520 times. Participants were aged 14 to 26 y and were scanned on 1 to 3 occasions at least 6 mo apart. We found 2 distinct modes of age-related change in FC: “conservative” and “disruptive.” Conservative development was characteristic of primary cortex, which was strongly connected at 14 y and became even more connected in the period from 14 to 26 y. Disruptive development was characteristic of association cortex and subcortical regions, where connectivity was remodeled: connections that were weak at 14 y became stronger during adolescence, and connections that were strong at 14 y became weaker. These modes of development were quantified using the maturational index (MI), estimated as Spearman’s correlation between edgewise baseline FC (at 14 y, FC14) and adolescent change in FC (ΔFC14−26), at each region. Disruptive systems (with negative MI) were activated by social cognition and autobiographical memory tasks in prior fMRI data and significantly colocated with prior maps of aerobic glycolysis (AG), AG-related gene expression, postnatal cortical surface expansion, and adolescent shrinkage of cortical thickness. The presence of these 2 modes of development was robust to numerous sensitivity analyses. We conclude that human brain organization is disrupted during adolescence by remodeling of FC between association cortical and subcortical areas

Structural features and immunological perception of the cell surface glycans of Lactobacillus plantarum: a novel rhamnose-rich polysaccharide and teichoic acids

Abstract The capsular material from Lactobacillus plantarum IMB19, an isolate from fermented vegetables, has been analyzed and our results demonstrate that most of the coat of this bacterium consists of glycerol- and ribitol-type teichoic acids, further decorated with other substituents (α-glucose and alanine), and of a capsular polysaccharide (CPS) with a linear nonasaccharide repeating unit, rich in rhamnose, interconnected to the next via a phosphodiester bridge. Stimulation of immune cells with the total capsular material resulted in the enhancement of immunostimulatory (IFNγ, TNF-α, IL-6 and IL-12) or immuno-regulatory (IL-10) cytokines in an in vitro splenocyte culture system. The capsular polysaccharide, and not the teichoic acids mixture, was responsible for the IFNγ production. Indeed, a significant increase of IFNγ along with other inflammatory cytokines, and a dose response in IFNγ expression with an EC50 of 3.16 μM was found for CPS, disclosing that this polysaccharide is a potent immunostimulatory molecule

Parallel Tempering: Theory, Applications, and New Perspectives

We review the history of the parallel tempering simulation method. From its origins in data analysis, the parallel tempering method has become a standard workhorse of physiochemical simulations. We discuss the theory behind the method and its various generalizations. We mention a selected set of the many applications that have become possible with the introduction of parallel tempering and we suggest several promising avenues for future research.Comment: 21 pages, 4 figure

The long-term effects of CVDs on economic development: the case of Bangalore

The aim of this paper is to demonstrate the economic and health burden of Cardiovascular diseases (CVDs) in the context of Bangalore during the period 2010-2013. A data set containing in excess of 1 million historic records corresponding to all-cause mortality was obtained from the central repository data register. After a data quality assessment, a dataset of n=183,893 was obtained. Spatial analysis was carried out to highlight the hotspots of CVD. Potential Years of Life Lost due to CVD and Present Value of Lifetime Earnings were computed. CVDs were responsible for 25% of the total of potential years of life lost. The potential value of lifetime earnings highlighted a loss in excess of 8 billion INR over the four years. CVD poses a tremendous challenge for socio-economic development, and there is an urgent need for a strategic action to promote CVD prevention and enable a sustainable development for the economy

Entrainment and development of larval fish assemblages in two contrasting cold core eddies of the East Australian Current system

Cyclonic eddies are diverse in their size, age, upwelling and behaviour, which has significant implications for fisheries production and connectivity when they interact with the continental shelf. To ascertain coastal entrainment by eddies, we compared the larval fish community of 2 contrasting cyclonic eddies in 3 depth strata (0-5, 5-50, 50-100 m), and with the adjacent shelf community. The frontal cyclonic eddy was smaller and younger than the mesoscale cyclonic eddy. A larval fish entrainment index, based on the ratio of coastal to oceanic taxa, revealed the relative abundance of coastal larvae entrained into the upper mixed layer of the frontal eddy, consistent with published numerical modelling studies of similar eddies. The frontal eddy had a high abundance of commercially important coastal taxa entrained from the inner shelf. However, the adjacent inner shelf water and putative location for frontal eddy formation had recently been displaced by the East Australian Current, resulting in the larval fish community being dominated by oceanic taxa. The spatial and temporal dynamics of coastal entrainment into the larger, older cyclonic eddy and the adjacent shelf region were revealed by mixtures of coastal and oceanic taxa in each of the depth strata. The larger cyclonic eddy had a higher biomass of zooplankton, indicating the cumulative effects of eddy age and production. Eddies which interact with the shelf en able cross-shelf mixing and may contribute to coastal fisheries

Synthesis and biological activity of α-glucosyl C24:0 and C20:2 ceramides

a-Glucosyl ceramides 4 and 5 have been synthesised and evaluated for their ability to stimulate the activation and expansion of human iNKT cells. The key challenge in the synthesis of both target molecules was the stereoselective synthesis of the a-glycosidic linkage. Of the methods examined, glycosylation using per-TMS-protected glucosyl iodide 16 was completely a-selective and provided gram quantities of amine 11, from which a-glucosyl ceramides 4 and 5 were obtained by N-acylation. a-GlcCer 4, containing a C24 saturated acyl chain, stimulated a marked proliferation and expansion of human circulating iNKT cells in short-term cultures. a-GlcCer 5, which contains a C20 11,14-cis-diene acyl chain (C20:2),induced extremely similar levels of iNKT cell activation and expansion

International Collaboration Towards Cambodia’s First Small Satellite Education Program: Lessons Learnt

Since 2019, students from the University of Tokyo (UTokyo) and the Institute of Technology of Cambodia (ITC) have collaborated to set up Cambodia’s first small satellite education program. Around 30 students from both institutions have organized and taken part in joint educational activities, articulated around the development of a 1U CubeSat, which is planned to become Cambodia’s first satellite. This paper presents lessons learnt so far during the cooperative education program. We first explain how students have led the collaboration between a mentor (UTokyo) and mentee (ITC) institution in different countries, mostly online due to the pandemic. We then provide transferable lessons on how to create a small satellite education program in a resource-scarce environment, based on the development of CubeSat training at the ITC. Finally, we present ongoing methods at the ITC to cultivate a supporting ecosystem for the new satellite education program. It is hoped that this paper will provide a useful reference point for other actors building a new and sustainable satellite education program with limited resources

Requirement of RIZ1 for cancer prevention by methyl-balanced diet

The typical Western diet is not balanced in methyl nutrients that regulate the level of the methyl donor S-adenosylmethionine (SAM) and its derivative metabolite S-adenosylhomocysteine (SAH), which in turn may control the activity of certain methyltransferases. Feeding rodents with amino acid defined and methyl-imbalanced diet decreases hepatic SAM and causes liver cancers. RIZ1 (PRDM2 or KMT8) is a tumor suppressor and functions in transcriptional repression by methylating histone H3 lysine 9. Here we show that a methyl-balanced diet conferred additional survival benefits compared to a tumor-inducing methyl-imbalanced diet only in mice with wild type RIZ1 but not in mice deficient in RIZ1. While absence of RIZ1 was tumorigenic in mice fed the balanced diet, its presence did not prevent tumor formation in mice fed the imbalanced diet. Unlike most of its related enzymes, RIZ1 was upregulated by methyl-balanced diet. Methyl-balanced diet did not fully repress oncogenes such as c-Jun in the absence of RIZ1. The data identify RIZ1 as a critical target of methyl-balanced diet in cancer prevention. The molecular understanding of dietary carcinogenesis may help people make informed choices on diet, which may greatly reduce the incidence of cancer