Oligocene Deep Water Export from the North Atlantic and the Development of the Antarctic Circumpolar Current Examined with Neodymium Isotopes

Neodymium (Nd) isotopes were measured on 181 samples of fossil fish teeth recovered from Oligocene toMiocene sections at Ocean Drilling Program Site 1090 (3700 m water depth) on Agulhas Ridge in the Atlanticsector of the Southern Ocean. A long-term decreasing trend toward less radiogenic Nd isotope compositionsdominates the record. This trend is interrupted by shifts toward more radiogenic compositions near the early/lateOligocene boundary and the Oligocene/Miocene boundary. Overall, eNd values at Agulhas Ridge are moreradiogenic than at other Atlantic locations, and are similar to those at Indian Ocean locations. The pattern ofvariability is remarkably similar to Nd isotope results from Walvis Ridge (South Atlantic) and Ninetyeast Ridge(Indian Ocean). In contrast, Agulhas Ridge and Maud Rise Nd isotope records do not show similar patterns overthis interval. Results from this study indicate that deep water in the Atlantic flowed predominantly from north tosouth during the Oligocene and Miocene, and that export of Northern Component Water (NCW) to the SouthernOcean increased in the late Oligocene. There is also evidence for efficient exchange of deep waters between theAtlantic sector of the Southern Ocean and the Indian Ocean, although the direction of deep water flow is notentirely clear from these data. The shifts to more radiogenic Nd isotopic compositions most likely representincreases in the flux of Pacific waters through Drake Passage, and the timing of these events reflect developmentof a mature Antarctic Circumpolar Current (ACC). The relative timing of increased NCW export and ACCmaturation support hypotheses that link deep water formation in the North Atlantic to the opening of DrakePassage

Eocene to Miocene Magnetostratigraphy, Biostratigraphy, and Chemostratigraphy at ODP Site 1090 (Sub-Antarctic South Atlantic)

At Ocean Drilling Program (ODP) Site 1090 (lat 42854.89S, long 8854.09E) locatedin a water depth of 3702 m on the Agulhas Ridge in the sub-Antarctic South Atlantic, ~300 m of middle Eocene to middle Miocenesediments were recovered with the advancedpiston corer (APC) and the extendedcore barrel (XCB). U-channel samplesfrom the 70–230 meters composite depth(mcd) interval provide a magnetic polaritystratigraphy that is extended to 380 mcd byshipboard whole-core and discrete sampledata. The magnetostratigraphy can be interpretedby the fit of the polarity-zone patternto the geomagnetic polarity time scale(GPTS) augmented by isotope data andbioevents with documented correlation tothe GPTS. Three normal-polarity subchrons(C5Dr.1n, C7Ar.1n, and C13r.1n),not included in the standard GPTS, are recordedat Site 1090. The base of the sampledsection is correlated to C19n (middleEocene), although the interpretation is unclearbeyond C17r. The top of the sampledsection is correlated to C5Cn (late earlyMiocene), although, in the uppermost 10 m of the sampled section, a foraminifer (Globorotaliasphericomiozea) usually associatedwith the Messinian and early Pliocene hasbeen identified. 87Sr/86Sr, d13C, and d18Ovalues measured on foraminifera, includingthe d18O and d13C shifts close to the Eocene/Oligocene boundary, support the correlationto the GPTS. For the interval spanningthe Oligocene/Miocene boundary, benthicd13C, d18O, and 87Sr/86Sr records from Site1090 can be correlated to isotope recordsfrom ODP Site 929 (Ceara Rise), providing support for the recently-published Oligocene/Miocene boundary age (22.92 Ma) of Shackleton et al

A Nd Isotopic Study of Southern Sourced Waters and Indonesian Throughflow at Intermediate Depths in the Cenozoic Indian Ocean

We present Nd isotopic data for fossil fish teeth recovered from the past 40 m.y. at ODP Site 757, currentlylocated at 1650m water depth on the Ninetyeast Ridge in the Indian Ocean. Although Site 757 sits in a regionstrongly influenced by weathering inputs from the Himalayas and volcanic inputs from the Indonesian arc, the pattern of Nd isotopic variations does not appear to respond to these potential sources of Nd. Instead, secular variations correlate to changes in the composition of intermediate to deep water masses bathing thesite and circulation patterns in the Indian Ocean. From ~40 to 10 Ma, eNd values and the pattern of change at Site 757 closely match those of ODP Site 1090, a deep water site in the Atlantic sector of the Southern Ocean. Comparison to data from several Fe-Mn crusts in the Indian Ocean suggests that intermediate to deep waterflow paths were similar to the modern distribution of Circumpolar DeepWater. At approximately 10 Ma, Ndisotopic values increase in a step function by 2 eNd units, suggesting that plate motions had carried Site 757into a region influenced by Indonesian Throughflow. Estimates of the vertical and horizontal position of thissite at 10 Ma imply that Indonesian Throughflow extended as far south as ~20S and to a depth of ~1500 m. From 10 to 0 Ma, Nd isotopic variations at Site 757 appear to record variations in Indonesian Throughflow. From 10 to 5.5 Ma, values at Site 757 overlap with those from crusts located in the southwest Pacific,indicating extensive flow through the Indonesian Seaway. From 5.5 to 3.4 Ma, eNd values become lessradiogenic at Site 757 and more radiogenic in the southwest Pacific, suggesting increasing closure of theseaway and concomitant rerouting of equatorial Pacific waters. Beginning at 3.4 Ma, eNd values becomemore radiogenic again at Site 757, which may be attributed to enhanced opening of the seaway or to a changein the source of Throughflow waters from a southern to a northern Pacific region

Evidence for Changes in Subsurface Circulation in the Late Eocene Equatorial Pacific from Radiolarian-Bound Nitrogen Isotope Values

Microfossil-bound organic matter represents an important archive of surface ocean environmental information. Sedimentary nitrogen (N) isotope reconstructions of surface nitrate consumption and nitrogen source changes are made using fossil diatom (autotrophs) and planktic foraminiferal (heterotrophs)-bound organic matter with success. However, because diatoms and planktic foraminifera are poorly preserved and sedimentary organic matter content is near zero during the late Eocene, our ability to examine nutrient dynamics across this important climate transition is limited. Here we present new data exploring the use of N isotope records from radiolarian tests. A comparison of surface ocean nitrate and core top bulk and radiolarian N isotope values (as δ15N) from the equatorial Pacific indicates that radiolarian-N records δ15N variability with fidelity but that a significant offset exists between bulk sedimentary and diatom δ15N values and those measured from radiolarians (~7.1 ± 1.1‰). A downcore profile of radiolarian δ15N values is compared to siliceous microfossil assemblage changes across the Eocene-Oligocene boundary. Average of radiolarian-bound δ15N values is 0.5 ± 2.0‰, which, when corrected using the offset derived from the modern surface samples, suggests that the mean nitrogen isotopic composition of the early Cenozoic eastern Pacific was not significantly different from today. The overall trend, of decreasing δ15N values with decreasing export productivity, is consistent with either a regional decline in pelagic denitrification or a large-scale change in nutrient sources to the eastern equatorial Pacific (EEP), both linked to the cooling climate and changing intermediate water circulation. Decreasing/low δ15N values cooccur with high radiolarian species turnover at ~35.5 and 34 Ma, suggestive of a significant ecological change in the EEP, consistent with cooling and water mass distribution changes. The preliminary results suggest that radiolarian-bound organic nitrogen represents another promising archive and underscores the fact that the different microfossil fractions must be separated to ensure robust results

The Use of Flagella and Motility for Plant Colonization and Fitness by Different Strains of the Foodborne Pathogen Listeria monocytogenes

The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either flaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the flaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a flaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the flaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases