Pollen development in Annona cherimola Mill. (Annonaceae). Implications for the evolution of aggregated pollen

Implications for the evolution of aggregated polle

Molecular Characterization of Apricot Germplasm from an Old Stone Collection

Increasing germplasm erosion requires the recovery and conservation of traditional cultivars before they disappear. Here we present a particular case in Spain where a thorough prospection of local fruit tree species was performed in the 1950s with detailed data of the origin of each genotype but, unfortunately, the accessions are no longer conserved in ex situ germplasm collections. However, for most of those cultivars, an old stone collection is still preserved. In order to analyze the diversity present at the time when the prospection was made and to which extent variability has been eroded, we developed a protocol in apricot (Prunus armeniaca L.) to obtain DNA from maternal tissues of the stones of a sufficient quality to be amplified by PCR. The results obtained have been compared with the results from the profiles developed from apricot cultivars currently conserved in ex situ germplasm collections. The results highlight the fact that most of the old accessions are not conserved ex situ but provide a tool to prioritize the recovery of particular cultivars. The approach used in this work can also be applied to other plant species where seeds have been preserved

Self-incompatibility in apricot: identifying pollination requirements to optimize fruit production

In recent years, an important renewal of apricot cultivars is taking place worldwide, with the introduction of many new releases. Self-incompatible genotypes tolerant to the sharka disease caused by the plum pox virus (PPV), which can severely reduce fruit production and quality, are being used as parents in most breeding programs. As a result, the self-incompatibility trait present in most of those accessions can be transmitted to the offspring, leading to the release of new self-incompatible cultivars. This situation can considerably affect apricot management, since pollination requirements were traditionally not considered in this crop and information is lacking for many cultivars. Thus, the objective of this work was to determine the pollination requirements of a group of new apricot cultivars by molecular identification of the S-alleles through PCR amplification of RNase and SFB regions with different primer combinations. The S-genotype of 66 apricot cultivars is reported, 41 for the first time. Forty-nine cultivars were considered self-compatible and 12 self-incompatible, which were allocated in their corresponding incompatibility groups. Additionally, the available information was reviewed and added to the new results obtained, resulting in a compilation of the pollination requirements of 235 apricot cultivars. This information will allow an efficient selection of parents in apricot breeding programs, the proper design of new orchards, and the identification and solution of production problems associated with a lack of fruit set in established orchards. The diversity at the S-locus observed in the cultivars developed in breeding programs indicates a possible genetic bottleneck due to the use of a reduced number of parents

Editorial: Breeding Innovations in Underutilized Temperate Fruit Trees

The recent growing interest in minor species (i.e., fig, pomegranate, feijoa, etc.) has recently driven new research on breeding and genetics to address producer and consumer traits. Since these species have received little attention from the scientific community, they were less improved via conventional breeding, and lacked detailed genomic information on important traits. This lack of data, together with a general poor genetic knowledge of these species, has limited a wider cultivation of varieties with improved characteristics

901-5 Intravenous Amiodarone Restores Sinus Rhythm in Acute Myocardial Infarction Complicated with Atrial Fibrillation

The effectiveness and tolerance of intravenous (IV) amiodarone (Am) in atrialfibrillation (AF) complicated acute myocardial infarction (AMI) is not well studied. Thus, twenty patients (Pts) with AMI complicated with AF occurring within 30h (11.9 ± 10) of the onset of AMI symptoms were treated with IV administration of digitalis (d, 0.5mg and an additional 0.25mg later) followed by IV Am 300mg over 2h (starting 2h after the initial dose of d) and followed by 44mg/h for up to 3 days, if sinus rhythm (SR) was not restored. Intravenous d restored SR within 2h in 5/20pts. AF relapsed in 2 of them. Subsequent administration of Am for 2 h restored SR in the remaining 15/20pts and in the 2pts in whom AF had relapsed after the initial restoration of SR by d. Am restored SR within an average of 12.8 (range 0.5–56) h of infusion. Total dose of Am was 1922 ± 720mg in 4pts and 425 ± 241mg in the remaining 13. Am was well tolerated by all pts including 1 with cardiogenic shock assisted with the intraaortic balloon pump. In conclusion, IV Am administration ishighly effective in restoring sinusrhythm in AF complicating AMI and is well tolerated

Albaricoquero: renovación varietal y necesidades de polinización

La reconversión varietal que se está produciendo en el albaricoquero es la respuesta ante diversos problemas que ha presentado el cultivo en los últimos años. El rápido lanzamiento de nuevas variedades desarrolladas en programas de mejora de todo el mundo ha provocado que las necesidades de polinización sean un factor importante a tener cada vez más en cuenta en el cultivo

Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae)

Genome duplication or polyploidy is one of the main factors of speciation in plants. It is especially frequent in hybrids and very valuable in many crops. The genus Annona belongs to the Annonaceae, a family that includes several fruit tree crops, such as cherimoya (Annona cherimola), sugar apple (Annona squamosa), their hybrid atemoya (A. cherimola × A. squamosa) or pawpaw (Asimina triloba). In this work, genome content was evaluated in several Annona species, A. triloba and atemoya. Surprisingly, while the hybrid atemoya has been reported as diploid, flow cytometry analysis of a progeny obtained from an interspecific cross between A. cherimola and A. squamosa showed an unusual ploidy variability that was also confirmed karyotype analysis. While the progeny from intraspecific crosses of A. cherimola showed polyploid genotypes that ranged from 2.5 to 33%, the hybrid atemoyas from the interspecific cross showed 35% of triploids from a total of 186 genotypes analyzed. With the aim of understanding the possible implications of the production of non-reduced gametes, pollen performance, pollen size and frequency distribution of pollen grains was quantified in the progeny of this cross and the parents. A large polymorphism in pollen grain size was found within the interspecific progeny with higher production of unreduced pollen in triploids (38%) than in diploids (29%). Moreover, using PCR amplification of selected microsatellite loci, while 13.7% of the pollen grains from the diploids showed two alleles, 41.28% of the grains from the triploids amplified two alleles and 5.63% showed up to three alleles. This suggests that the larger pollen grains could correspond to diploid and, in a lower frequency, to triploid pollen. Pollen performance was also affected with lower pollen germination in the hybrid triploids than in both diploid parents. The results confirm a higher percentage of polyploids in the interspecific cross, affecting pollen grain size and pollen performance. The occurrence of unreduced gametes in A. cherimola, A. squamosa and their interspecific progeny that may result in abnormalities of ploidy such as the triploids and tetraploids observed in this study, opens an interesting opportunity to study polyploidy in Annonaceae

Discovery of non-climacteric and suppressed climacteric bud sport mutations originating from a climacteric Japanese plum cultivar (Prunus salicina Lindl.).

Japanese plums are classified as climacteric; however, some economically important cultivars selected in California produce very little ethylene and require long ripening both "on" and "off" the tree to reach eating-ripe firmness. To unravel the ripening behavior of different Japanese plum cultivars, ripening was examined in the absence (air) or in the presence of ethylene or propylene (an ethylene analog) following a treatment or not with 1-methylcyclopropene (1-MCP, an ethylene action inhibitor). Detailed physiological studies revealed for the first time three distinct ripening types in plum fruit: climacteric, suppressed-climacteric, and non-climacteric. Responding to exogenous ethylene or propylene, the slow-softening supressed-climacteric cultivars produced detectable amounts of ethylene, in contrast to the novel non-climacteric cultivar that produced no ethylene and softened extremely slowly. Genetic analysis using microsatellite markers produced identical DNA profiles for the climacteric cultivars "Santa Rosa" and "July Santa Rosa," the suppressed-climacteric cultivars "Late Santa Rosa," "Casselman," and "Roysum" and the novel non-climacteric "Sweet Miriam," as expected since historic records present most of these cultivars as bud-sport mutations derived initially from "Santa Rosa." This present study provides a novel fruit system to address the molecular basis of ripening and to develop markers that assist breeders in providing high-quality stone fruit cultivars that can remain "on-tree," increasing fruit flavor, saving harvesting costs, and potentially reducing the need for low-temperature storage during postharvest handling