Faktory ovlivňující pohyb a chování živočichů v říčním prostředí

Tekoucí vody představují velmi variabilní prostředí a organismy, které je obývají, využívají různé adaptační mechanismy, které umožňují jejich existenci a rozmnožování. Chování a migrace živočichů patří mezi klíčové mechanismy umožňující přizpůsobení k podmínkám prostředí a jejich změnám. Ve své disertační práci jsem se zabývala zejména abiotickými a antropogenními faktory, které ovlivňují časovou a prostorovou distribuci a chování živočichů v říčním prostředí. Disertační práce se skládá ze dvou částí: první část je složena ze tří kapitol, které zkoumají které abiotické faktory a jakým způsobem ovlivňují chování živočichů. První dvě kapitoly presentují výsledky rozsáhlých telemetrických studií, popisujících diurnální chování a migrace jelce jesena (Leuciscus idus) a lipana podhorního (Thymallus thymallus). První kapitola představuje výsledky ročního sledování diurnální a sezónní variability v chování jelce jesena v řece Labi, druhá kapitola je výsledkem tříletého sledování juvenilních lipanů v průběhu podzimního a zimního období. V této studii jsme se dále zaměřili na rozdílnosti v chování divokých lipanů a lipanů ze sádek. Třetí kapitola zkoumá diurnální chování a šíření invazního korýše, pontokaspického vidlonožce Hemimysis anomala. Druhá část disertační práce zahrnuje dvě publikace založené na...Animals living in riverine environments are influenced by various abiotic and biotic factors which shape their behaviour. Among the most distinctive behavioural traits by which animals react and adapt to environmental variability is movement behaviour. In my study I focused on movement and distribution of several species and their response to environmental and anthropogenic factors. The thesis consists of two parts: the first part synthesizes studies on behaviour of European grayling Thymallus thymallus, Ide Leuciscus idus and an invasive crustacean Hemimysis anomala; the objectives of individual projects were to determine which environmental factors influence the species' behaviour and how. The first two chapters present extensive radio-telemetry studies which examined diel movements and migration of ide (one year observation in the River Elbe) and European grayling (study conducted in autumn-winter periods of three consecutive years in the River Blanice, focusing on differences between juveniles of wild and hatchery origin). The third study was also conducted in the Elbe and evaluated the diurnal behavioural patterns and spread of the Pontocaspian mysid shrimp Hemimysis anomala. The second part of the thesis consists of two chapters focusing on young-of-the-year (YOY) fish assemblages: the first...Katedra ekologieDepartment of EcologyFaculty of SciencePřírodovědecká fakult

Sports hall

V diplomové práci je vypracován ve dvou variantách návrh nosné konstrukce sportovní haly pro běžné sporty (házená, malý fotbal, tenis, volejbal, košíková) o půdorysných rozměrech 42 m x 72 m a světlé výšce 8 m. Konstrukce je navržena pro oblast Brno. Ve statickém výpočtu jsou navrženy a posouzeny hlavní nosné konstrukční prvky jako dřevěný lepený plnostěnný vazník, dřevěné vaznice, ocelové trámky, ztužidla a táhla, sloupy, které spolu s vazníkem tvoří příčnou vazbu, dále patka a kotvení. Vybraná varianta je dále podrobněji rozpracována. Součástí práce je výkresová dokumentace vybrané varianty skládající se z dispozičního výkresu, výkresu vybraných konstrukčních dílců a charakteristické detaily.The goal of this master´s thesis is to design load bearing structure of a sports hall. The thesis is elaborated in two variants of the structure design of the sports hall for common types of sports (handball, footsal, tennis, volleyball, basketball). The ground plan dimension is 42 to 72 meters and the construction height is 8 meters. The construction is designed for the district of Brno city. In the structural anlysis there were the following main load bearing structural elements designed and examined: glue laminated timber truss, wood purlins, steel beams, rods and bracing, columns, which together with the transverse girders are linked and foot and anchoring. The selected variant is also further developed. Part of the work is drawing documentation of the choosen variant, disposition drawing and drawings of the selected details.

Bioaccumulation of chemical elements at post-industrial freshwater sites varies predictably between habitats, elements and taxa: A power law approach

Elevated environmental levels of elements originating from anthropogenic activities threaten natural communities and public health, as these elements can persist and bioaccumulate in the environment. However, their environmental risks and bioaccumulation patterns are often habitat-, species- and element-specific. We studied the bioaccumulation patterns of 11 elements in seven freshwater taxa in post-mining habitats in the Czech Republic, ranging from less polluted mining ponds to highly polluted fly ash lagoons. We found nonlinear, power-law relationships between the environmental and tissue concentrations of the elements, which may explain differences in bioaccumulation factors (BAF) reported in the literature. Tissue concentrations were driven by the environmental concentrations in non-essential elements (Al, As, Co, Cr, Ni, Pb and V), but this dependence was limited in essential elements (Cu, Mn, Se and Zn). Tissue concentrations of most elements were also more closely related to substrate than to water concentrations. Bioaccumulation was habitat specific in eight elements: stronger in mining ponds for Al and Pb, and stronger in fly ash lagoons for As, Cu, Mn, Pb, Se, V and Zn, although the differences were often minor. Bioaccumulation of some elements further increased in mineral-rich localities. Proximity to substrate, rather than trophic level, drove increased bioaccumulation levels across taxa. This highlights the importance of substrate as a pollutant reservoir in standing freshwaters and suggests that benthic taxa, such as molluscs (e.g., Physella) and other macroinvertebrates (e.g., Nepa), constitute good bioindicators. Despite the higher environmental risks in fly ash lagoons than in mining ponds, the observed ability of freshwater biota to sustain pollution supports the conservation potential of post-industrial sites. The power law approach used here to quantify and disentangle the effects of various bioaccumulation drivers may be helpful in additional contexts, increasing our ability to predict the effects of other contaminants and environmental hazards on biota.info:eu-repo/semantics/publishedVersio

Three major steps toward the conservation of freshwater and riparian biodiversity

J.M. and J.H. thank Stiftelsen Längmanska kulturfonden for funding travel to the conference. As.L. and K.R. thank the Estonian Research Council (grant 1121) for financial support, and A.M. acknowledges the Leverhulme Trust (RPG-2019-402). H.H. was supported by the European Union Horizon 2020 Research and Innovation Programme under the Marie Sklodowska-Curie Actions (grant agreement 860800): RIBES (river flow regulation, fish behaviour, and status), and V.A. acknowledges the support from the Leibniz Competition project Freshwater Megafauna Futures. E.J. received support through the National Laboratory for Health Security (RRF-2.3.1-21-2022-00006), Centre for Ecological Research, Budapest, Hungary, and thanks Z. Molnár for support.Freshwater ecosystems and their bordering wetlands and riparian zones are vital for human society and biological diversity. Yet, they are among the most degraded ecosystems, where sharp declines in biodiversity are driven by human activities, such as hydropower development, agriculture, forestry, and fisheries. Because freshwater ecosystems are characterized by strongly reciprocal linkages with surrounding landscapes, human activities that encroach on or degrade riparian zones ultimately lead to declines in freshwater–riparian ecosystem functioning. We synthesized results of a symposium on freshwater, riparian, and wetland processes and interactions and analyzed some of the major problems associated with improving freshwater and riparian research and management. Three distinct barriers are the lack of involvement of local people in conservation research and management, absence of adequate measurement of biodiversity in freshwater and riparian ecosystems, and separate legislation and policy on riparian and freshwater management. Based on our findings, we argue that freshwater and riparian research and conservation efforts should be integrated more explicitly. Best practices for overcoming the 3 major barriers to improved conservation include more and sustainable use of traditional and other forms of local ecological knowledge, choosing appropriate metrics for ecological research and monitoring of restoration efforts, and mirroring the close links between riparian and freshwater ecosystems in legislation and policy. Integrating these 3 angles in conservation science and practice will provide substantial benefits in addressing the freshwater biodiversity crisis.Peer reviewe

Spexin and nesfatin-1-expressing neurons in the male human claustrum

Neuropeptides are involved in numerous brain activities being responsible for a wide spectrum of higher mental functions. The purpose of this concise, structural and qualitative investigation was to map the possible immunoreactivity of the novel regulatory peptides: spexin (SPX) and nesfatin-1 within the human claustrum. SPX is a newly identified peptide, a natural ligand for the galanin receptors (GALR) 2/3, with no molecular structure similarities to currently known regulatory factors. SPX seems to have multiple physiological functions, with an involvement in reproduction and food-intake regulation recently revealed in animal studies. Nesfatin-1, a second pleiotropic neuropeptide, which is a derivative of the nucleobindin-2 (NUCB-2) protein, is characterized by a wide distribution in the brain. Nesfatin-1 is a substance with a strong anorexigenic effect, playing an important role in the neuronal circuits of the hypothalamus that regulate food intake and energy homeostasis. On the other hand, nesfatin-1 may be involved in several important brain functions such as sleep, reproductive behaviour, cognitive processes, stress responses and anxiety. For the first time we detected and described a population of nesfatin-1 and SPX expressing neurons in the human claustrum using immunohistochemical and fluorescent methods. The study presents the novel identification of SPX and nesfatin-1 immunopositive neurons in the human claustrum and their assemblies show similar patterns of distribution in the whole structure

Structural Characterization of Unusual Fatty Acid Methyl Esters with Double and Triple Bonds Using HPLC/APCI-MS2 with Acetonitrile In-Source Derivatization

Double and triple bonds have significant effects on the biological activities of lipids. Determining multiple bond positions in their molecules by mass spectrometry usually requires chemical derivatization. This work presents an HPLC/MS method for pinpointing the double and triple bonds in fatty acids. Fatty acid methyl esters were separated by reversed-phase HPLC with an acetonitrile mobile phase. In the APCI source, acetonitrile formed reactive species, which added to double and triple bonds to form [M + C3H5N]+• ions. Their collisional activation in an ion trap provided fragments helpful in localizing the multiple bond positions. This approach was applied to fatty acids with isolated, cumulated, and conjugated double bonds and triple bonds. The fatty acids were isolated from the fat body of early-nesting bumblebee Bombus pratorum and seeds or seed oils of Punicum granatum, Marrubium vulgare, and Santalum album. Using the method, the presence of the known fatty acids was confirmed, and new ones were discovered

Factors influencing movement and behaviour of animals in riverine environment

Animals living in riverine environments are influenced by various abiotic and biotic factors which shape their behaviour. Among the most distinctive behavioural traits by which animals react and adapt to environmental variability is movement behaviour. In my study I focused on movement and distribution of several species and their response to environmental and anthropogenic factors. The thesis consists of two parts: the first part synthesizes studies on behaviour of European grayling Thymallus thymallus, Ide Leuciscus idus and an invasive crustacean Hemimysis anomala; the objectives of individual projects were to determine which environmental factors influence the species' behaviour and how. The first two chapters present extensive radio-telemetry studies which examined diel movements and migration of ide (one year observation in the River Elbe) and European grayling (study conducted in autumn-winter periods of three consecutive years in the River Blanice, focusing on differences between juveniles of wild and hatchery origin). The third study was also conducted in the Elbe and evaluated the diurnal behavioural patterns and spread of the Pontocaspian mysid shrimp Hemimysis anomala. The second part of the thesis consists of two chapters focusing on young-of-the-year (YOY) fish assemblages: the first..

Nursing casuistry about the patient with cancer of the endometrium

Ústav teorie a praxe ošetřovatelství 1. LF UK v PrazeInstitute of Theory and Practice of Nursing First Faculty of Medicine Charles University in PragueFirst Faculty of Medicine1. lékařská fakult