Transposição dentária: dois relatos de casos e revisão de literatura

Tooth transposition is a rare developmental disorder in which a normal tooth erupts in an inappropriate position. The change in position occurs between two adjacent teeth within the same quadrant. In this article, two cases of tooth transposition involving mandibular molars are described. Patient 1 presented with bilateral transposition between first and second mandibular molars. In addition, giroversion of the first molars was observed. Patient 2 presented with unilateral transposition between the mandibular left molars. Because this tooth disorder does not affect quality of life, both patients declined to seek dental treatment. Out of the 120 cases of tooth transposition found in the literature, 92,5% occurred in the maxilla and 15% were bilateral, 65,8% percent of these cases involved the canine and first premolar; 26.6% involved the canine and lateral incisor; 2,5% involved the canine and central incisor; and only 0.85% involved the first and second molar. The majority of tooth transposition cases occurred in women (66,6%). In literature, tooth transposition is a poorly-documented condition. Consequently, this report seeks to contribute to the existing information in the literature on this disorder.Resumo em português não disponíve

Gene Expression Analysis of the Hepatotoxicant Methapyrilene in Primary Rat Hepatocytes: An Interlaboratory Study

Genomics technologies are used in several disciplines, including toxicology. However, these technologies are relatively new, and their applications require further investigations. When investigators apply these technologies to in vitro experiments, two major issues need to be clarified: a) can in vitro toxicity studies, in combination with genomics analyses, be used to predict the toxicity of a compound; and b) are the generated toxicogenomics data reproducible between laboratories? These questions were addressed by an interlaboratory study with laboratories of four pharmaceutical companies. We evaluated gene expression patterns from cultured rat primary hepatocytes after a 24-hr incubation with methapyrilene (MP). Extensive data analysis showed that comparison of genomics data from different sources is complex because both experimental and statistical variability are important confounding factors. However, appropriate statistical tools allowed us to use gene expression profiles to distinguish high-dose–treated cells from vehicle-treated cells. Moreover, we correctly identified MP in an independently generated in vitro database, underlining that in vitro toxicogenomics could be a predictive tool for toxicity. From a mechanistic point of view, despite the observed site-to-site variability, there was good concordance regarding the affected biologic processes. Several subsets of regulated genes were obtained by analyzing the data sets with one method or using different statistical analysis methods. The identified genes are involved in cellular processes that are associated to the exposure of primary hepatocytes to MP. Whether they are specific for MP and are cause or consequence of the toxicity requires further investigations

Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using integration free Sendai virus technology

We have generated a human induced pluripotent stem cell (iPSC) line derived from urinary cells of a 28 year old healthy female donor. The cells were reprogrammed using a non-integrating viral vector and have shown full differentiation potential. Together with the iPSC line, the donor provided blood cells for the study of immunological effects of the iPSC line and its derivatives in autologous and allogeneic settings. The line is available and registered in the human pluripotent stem cell registry as BCRTi005-A

Generation of integration free induced pluripotent stem cells from fibrodysplasia ossificans progressiva (FOP) patients from urine samples

Fibrodysplasia ossificans progressiva (FOP) is an extremely rare, autosomal dominant transmitted genetic disease. Patients experience progressive bone formation replacing tendons, ligaments, muscle and soft tissue. Cause of FOP are gain-of-function mutations in the Bone Morphogenetic Protein (BMP) receptor Activin A receptor type 1 (ACVR1) ( Kaplan et al., 2008). The most common mutation is R206H, which leads to the substitution of codon 206 from arginine to histidine (Shore et al., 2006). Here, we describe the derivation and characterization of two hiPSC lines from two FOP patients, both carrying the mutation R206H. Cells were isolated from urine and reprogrammed using integration free Sendai virus vectors under defined conditions

Keratoacanthoma associated to cutaneous horn manifestation: case report and difficulty of diagnosis

Background: Cutaneous horn (CH) is a conical hyperkatotic projection of skin with keratotic material. A broad variety of lesions may be found with clinical aspect of cutaneous horn, including malignant tumors.  Case report: We report a case of patient with 77- year-old male, presenting an asymptomatic lesion with clinical aspect of CH in lower lip. The clinical diagnosis hypothesis was squamous cell carcinoma (SCC) and an incisional biopsy was performed. The histopathological analysis revealed a keratoacantoma (KA) and the lesion was completely removed. The one year follow-up revealed no recurrence. Discussion: CH rarely is accompanied by KA. However, KA shows a greater degree of nuclear atypia than SCC making the differentiation of two diseases very difficult. This case highligh the importance of histopathological examination to rule out malignancy.Introdução: O corno cutâneo (CC) é uma projeção hiperceratótica cônica da pele. Uma ampla variedade de lesões podem ser encontrados com aspecto clínico de corno cutâneo, incluindo tumores malignos. Relato de caso: Relatamos um caso de paciente do sexo masculino, 77 anos de idade, apresentando uma lesão assintomática, com aspecto clínico da CC no lábio inferior. A hipótese de diagnóstico clínico foi de carcinoma espinocelular (CEC) e uma biópsia incisional foi realizada. A análise histopatológica revelou um ceratoacantoma e a lesão foi completamente removida. Em um ano de acompanhamento nenhuma recidiva foi observada. Discussão: CC raramente é acompanhada por ceratoacantoma. No entanto, ceratoacantoma mostra um maior grau de atipia nuclear do que CEC tornando a diferenciação de duas doenças muito difícil. Neste caso ressaltamos a importância do exame histopatológico para descartar malignidade

Evaluation of cell proliferation rate in non-dysplastic leukoplakias

Objective: Analyze whether the most frequent cases of non-dysplastic leukoplakias, hyperkeratosis (H), acanthosis (A), and hyperkeratosis with acanthosis (HA) have similar cell proliferation rates and to compare them with epithelial dysplastic (ED) leukoplakias and normal oral epithelium (NOE).Study design: The sample comprised 10 cases of normal oral epithelium, 10 cases of hyperkeratosis, 10 cases of acanthosis, 10 cases of hyperkeratosis with acanthosis and 10 cases of epithelial dysplasia. The mean number of AgNORs per nucleus (mAgNOR) and the mean percentage of cells with 1, 2, 3 and 4 or more AgNORs per nucleus (pAgNOR) were recorded. Results: The results of mAgNOR showed differences between disorders in the evaluation of the basal layer, of the parabasal layer, and in the overall evaluation. mAgNOR and pAgNOR=2 increased progressively from normal oral epithelium to hyperkeratosis with acanthosis, hyperkeratosis, acanthosis and epithelial dysplasia (p<0.05). Cell proliferation rate was different between different subtypes of non-dysplastic leukoplakias and this group presented a higher proliferative behavior when compared to normal oral epithelium. Conclusion: It may be suggested that non-dysplastic leukoplakias had different characteristics regarding cell proliferation rates and sometimes showed a proliferative behavior similar to that found in epithelial dysplasia. More studies should be conduced to increase knowledge about the biological profile of non-dysplastic leukoplakias, especially as it pertains to acanthosis

Overexpression of ALDH1 and EMT marker profile are linked with unfavorable outcome in head and neck cancer

The aim of this research was to assess the expression of aldehyde dehydrogenase 1 (ALDH1) and epithelial-mesenchymal transition (EMT) markers in head and neck squamous cell carcinoma (HNSCC), and to correlate them with the clinical and histopathological parameters of a patient cohort with follow-up over an 8-year period. For this, seventeen HNSCC and non-neoplastic adjacent epithelium (AE) samples were subjected to laser microdissection and real-time PCR to evaluate the mRNA expression of ALDH1, E-cadherin (E-CAD), N-cadherin (N-CAD), and vimentin (VIM). Also, immunohistochemistry was performed for ALDH1, E-CAD, N-CAD, and VIM in the tumor center (TC), invasion front (IF), and AE of the seventeen samples. Mann-Whitney, Kruskal-Wallis and Chi-square tests were used to correlate the mRNA and immunohistochemical expression with different variables, considering p<0.05. Kaplan-Meier curves were produced for local recurrence, regional metastasis and treatment. A mRNA overexpression of ALDH1 in primary tumors was associated with regional metastasis and a high ALDH1 immunostaining was related to metastasis and a worse patient outcome. Additionally, a favorable outcome was associated with the transition phase and an unfavorable outcome was associated with EMT event. An overall 26.9 months was observed with longer survival associated with surgery and radiotherapy. However, due to the intense variability inherent to the indicator proteins in the EMT process, the complete profile markers related to this biological process should be continuous investigated

Non-muscle myosin II as a predictive factor in head and neck squamous cell carcinoma

The present study attempted to provide information regarding non-muscle myosin II (MII) isoforms immunoreactivity in patients with head and neck squamous cell carcinoma (HNSCC) and analysis of the patients? clinical status after 5 years of monitoring. A semiquantitative analysis of the immunoreactivity of the MII isoforms was performed in 54 surgical specimens and its correlation with clinical and pathological variables and prognosis was verified. Data were analyzed using chi-square, Mann-Whitney and Kruskal-Wallis tests. To evaluate the survival over the total monitoring time and any connection with the proteins studied, the Kaplan-Meier analysis was used. P values ?0.05 were considered statistically significant. In the advanced stages of pathological tumor-node-metastasis, the expression of MIIB in adjacent non-neoplastic epithelial tissues tended to increase (p = 0.057). In tumoral zones there was an association of high expression among the three isoforms (MIIA/MIIB p=0,001, MIIB/MIIC p=0,006 and MIIA/MIIC p=0,012). Negative clinical evolution in patients was directly correlated to increased MIIC expression in the tumoral zone of invasion in HNSCC (p = 0.017). Based on clinical evolution after the monitoring period, patients with tumors expressing MIIC had poorer prognoses (p = 0.048). The present study suggests that MIIB expression in non-neoplastic adjacent epithelial tissues may indicate a potential for regional metastasis and that MIIC expression in the tumoral zone of invasion is predictive of negative evolution of the disease