High-speed video-oculography applied to assess pupil light reflex

Póster presentado en el VII European/ I World Meeting in Visual and Physiological OpticsEye response to light exposure is usually described through the pupillary light reflex, which controls the pupil diameter and allows for testing the sensory and motor functions of the eye. We have arranged an experimental setup and developed a procedure in order to improve the video-oculography experiment through high-speed imaging. The technique has been applied over eleven people distinguishing between consensual and direct pupillary light reflexes and analyzing the eye dominance. We found no significant differences. Moreover, obtained time parameters describing the pupil light reflex agree with those from literature.The authors acknowledge the projects BIA2011-22704, GV/2013/009 and PROMETEO/ 2011/021. A. B. Roig acknowledges a grant from Cajamurcia

A high-resolution binocular video-oculography system: assessment of pupillary light reflex and detection of an early incomplete blink and an upward eye movement

Background: The pupillary light reflex characterizes the direct and consensual response of the eye to the perceived brightness of a stimulus. It has been used as indicator of both neurological and optic nerve pathologies. As with other eye reflexes, this reflex constitutes an almost instantaneous movement and is linked to activation of the same midbrain area. The latency of the pupillary light reflex is around 200 ms, although the literature also indicates that the fastest eye reflexes last 20 ms. Therefore, a system with sufficiently high spatial and temporal resolutions is required for accurate assessment. In this study, we analyzed the pupillary light reflex to determine whether any small discrepancy exists between the direct and consensual responses, and to ascertain whether any other eye reflex occurs before the pupillary light reflex. Methods: We constructed a binocular video-oculography system two high-speed cameras that simultaneously focused on both eyes. This was then employed to assess the direct and consensual responses of each eye using our own algorithm based on Circular Hough Transform to detect and track the pupil. Time parameters describing the pupillary light reflex were obtained from the radius time-variation. Eight healthy subjects (4 women, 4 men, aged 24–45) participated in this experiment. Results: Our system, which has a resolution of 15 microns and 4 ms, obtained time parameters describing the pupillary light reflex that were similar to those reported in previous studies, with no significant differences between direct and consensual reflexes. Moreover, it revealed an incomplete reflex blink and an upward eye movement at around 100 ms that may correspond to Bell’s phenomenon. Conclusions: Direct and consensual pupillary responses do not any significant temporal differences. The system and method described here could prove useful for further assessment of pupillary and blink reflexes. The resolution obtained revealed the existence reported here of an early incomplete blink and an upward eye movement.The authors acknowledge the support of the Spanish Ministerio de Economía y Competitividad through the project BIA2011-22704 and the Generalitat Valenciana through the projects GV/2013/009 and PROMETEO/2011/021. A. B. Roig acknowledges a grant from Cajamurcia

Three-dimensional planar object tracking with sub-pixel accuracy

Subpixel techniques are commonly used to increase the spatial resolution in tracking tasks. Object tracking with targets of known shape permits obtaining information about object position and orientation in the three-dimensional space. A proper selection of the target shape allows us to determine its position inside a plane and its angular and azimuthal orientation under certain limits. Our proposal is demonstrated both numerical and experimentally and provides an increase the accuracy of more than one order of magnitude compared to the nominal resolution of the sensor. The experiment has been performed with a high-speed camera, which simultaneously provides high spatial and temporal resolution, so it may be interesting for some applications where this kind of targets can be attached, such as vibration monitoring and structural analysis.The authors acknowledge the support of the Spanish Ministerio de Economía y Competitividad through the project BIA2011-22704 and the Generalitat Valenciana through the projects GV/2013/009 and PROMETEO/ 2011/021. A. B. Roig acknowledges a grant from Cajamurcia

Effective focal length through a microscope

Póster presentado en el VII European/ I World Meeting in Visual and Physiological OpticsThe optical power of a thick spherical lens and its Coddington shape factor are essential magnitudes that characterize its image quality. We propose an experimental procedure and apparatus that allow accurate determination of those magnitudes for any spherical lens from geometrical measurements. It overcomes the drawbacks of other devices that need of the refractive index or may damage the lens surfaces, like spherometers, and provides similar results to commercial lensmeters.The authors acknowledge the projects BIA2011-22704, GV/2013/009 and PROMETEO/ 2011/021. J. Espinosa thanks R. Tomás the provisioning. A. B. Roig acknowledges a grant from Cajamurcia

EDUCACIÓN AMBIENTAL Y SOCIEDAD. SABERES LOCALES PARA EL DESARROLLO Y LA SUSTENTABILIDAD

Este texto contribuye al análisis científico de varias áreas del conocimiento como la filosofía social, la patología, la educación para el cuidado del medio ambiente y la sustentabilidad que inciden en diversas unidades de aprendizaje de la Licenciatura en Educación para la Salud y de la Maestría en Sociología de la SaludLas comunidades indígenas de la sierra norte de Oaxaca México, habitan un territorio extenso de biodiversidad. Sin que sea una área protegida y sustentable, la propia naturaleza de la región ofrece a sus visitantes la riqueza de la vegetación caracterizada por sus especies endémicas que componen un paisaje de suma belleza

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Analysis of fixational eye micromovements through pupil tracking

Póster presentado en OPTYKA Optical Fair 2012, Poznan, Polonia, 9-10 noviembre 2012.The human eye is constantly moving even when it is maintaining a stable fixation point. Stable images on the retina rapidly saturate the photoreceptors and fade. The visual system needs of constant stimulation is achieved by fixation micromovements consisting of microtremors, drifts and microsaccades. We present a technique for pupil segmentation and contour analysis which will provide valuable information about fixational eye movements.Thanks to Spanish Ministerio de Ciencia e Innovación through BIA2011-22704, the Generalitat Valenciana through the project PROMETEO/2011/021 and the University of Alicante through the project GRE10-09. A.B. Roig thanks Fundación Cajamurcia the grant “Beca de investigación Cajamurcia 2010

Kinematics of eyelid movement and eye retraction in the blinking

Resumen del póster expuesto en el 6th EOS Topical Meeting on Visual and Physiological Optics (EMVPO 2012), Dublín, 20-22 Agosto 2012.A non-invasive technique is used to measure the eyelid closure and simultaneous eye retraction. We have fitted the displacement of the eyelid to an analytical function and have extended the kinematic model to the eye retraction movements. As a result, some dynamic parameters have been presented.Spanish Ministerio de Ciencia e Innovación through the project FIS2009-05639-ET and BIA2011-22704, the Generalitat Valenciana through the project PROMETEO/2011/021 and the University of Alicante through the project GRE10-09. A.B. Roig thanks Fundación Cajamurcia the grant “Beca de investigación Cajamurcia 2010”

Tracking of fixational eye micromovements through pupil image segmentation

Póster y comunicación presentados en 5th European Meeting on Visual and Physiological Optics (EMVPO), Stockholm, 22-24 August 2010.We present a technique for pupil segmentation and contour analysis which will provide valuable information about fixational eye movements. The method is based on fitting an ellipse to the pupil contour. Pupillary hippus, microsaccades and drifts are obtained, as well as cyclotorsional movements.This work has been supported by the Generalitat Valenciana project nr. GV/2009/002