Antibiotic resistance and mecA characterization of coagulase-negative staphylococci isolated from three hotels in London, UK

Antibiotic resistance in bacteria isolated from non–healthcare environments, is a potential problem to public health. In our survey a total of 71 coagulase negative staphylococci belonging to 11 different species were isolated from 3 large hotels in London, UK. The most prevalent species was Staphylococcus haemolyticus, with S. hominis, S. warneri, S.cohnii and S. epidermidis commonly detected. Antimicrobial susceptibilities and carriage of the mecA gene were determined for all of these isolates. Most (85.9%) staphylococci were resistant to multiple antibiotics with all displaying increased susceptibility towards penicillin, fusidic acid, erythromycin and cefepime. 21 (29.5%) of the isolates were mecA positive, however MIC values to oxacillin, normally associated with the carriage of mecA, varied widely in this group (from 0.06 mg/L to 256 mg/L). 15 of the 21 mecA positive isolates carried SCCmec of these 7 were type V, 1 type I, 1 type II and 1 type IV. Additionally, five of these 15 isolates carried a previously unreported type, 1A, which involves an association between class A mec complex and ccr type 1. The remaining 6 of the 21 isolates were non-typeable and carried a combination of class A mec complex and ccrC. In addition to this, we also report on new MLST types which were assigned for 5 Staphylococcus epidermidis isolates. 4 out of these 5 isolates had MICs between 0.06 to 256 mg/L to oxacillin and would be regarded as clinically susceptible but one isolate had a high oxacillin MIC of 256 mg/L. We demonstrated widespread multiple drug resistance among different staphylococcal species isolated from non-healthcare environments highlighting the potential for these species to act as a reservoir for methicillin and other forms of drug resistance

Whole-genome sequencing and comparative genomics analysis of a newly emerged multidrug-resistant Klebsiella pneumoniae isolate of ST967

Whole-genome sequencing and population genetics analysis of K. pneumoniae are scarce from LMICs, and none has been reported for Armenia. Multilevel comparative analysis revealed that ARM01 (an isolate belonging to a newly emerged K. pneumoniae ST967 lineage) was genetically similar to two isolates recovered from Qatar

Could Public Restrooms Be an Environment for Bacterial Resistomes?

PMCID: PMC3547874This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

MALDI TOF MS and currently related proteomic technologies in reconciling bacterial systematics

The chapter is on development and application of matrix assisted laser desorption ionisation time of flight mass spectrometry (MALDI-TOF) to identification and and classification of bacteria

Summary of Family and Genera of bacteria identified by MALDI-TOF-MS.

*<p>Others include genera of <i>Korucia (6); Rothia (2); Arthrobacter (2); Anaerococcus (3); Rhodococcus (2)</i>.</p

Identification of Staphylococci isolates by MALDI-TOF MS and API ID 32 STAPH system compared with 16S RNA sequencing.

<p>Identification of Staphylococci isolates by MALDI-TOF MS and API ID 32 STAPH system compared with 16S RNA sequencing.</p

Concordance between conventional and direct and extracted MALDI-TOF-MS methods for Gram-negative isolates of three genera.

<p>D-MALDI-TOF (direct method); E-MALDI-TOF (extracted method); AE increase (increase of identification after alcoholic extraction).</p

Supplemental Material for Xu et al., 2018

<div>Figure S1. Pairwise allignments of the genome presented in this study; G6_2 compared against a) 949_S8; b) ATCC12228; c) BPH 0662; d) PM221, e) RP62A and e) SEI</div><div>TABLE S1: ASSEMBLY METRICS (GENERATED USING WEB QUAST), ALL STATISTICS ARE BASED ON CONTIGS OF SIZE >= 500 BP</div><div>TABLE S2: SIMILARITY MATRIX FOR EACH STRAIN IN THIS STUDY, G6 2 AND REFERENCE STRAINS</div><div><br></div