Phylogenetic diversity, antimicrobial susceptibility and virulence gene profiles of Brachyspira hyodysenteriae isolates from pigs in Germany

Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17.6, bhlp29.7, bhmp39f, and bhmp39h) as well as iron acquisition factors (ftnA and bitC). Multilocus sequence typing (MLST) revealed that 79.4% of the isolates belonged to only three STs, namely ST52 (41.4%), ST8 (12.1%), and ST112 (25.9%) which have been observed in other European countries before. Another 24 isolates belonged to twelve new STs (ST113-118, ST120-123, ST131, and ST193). The temporal distribution of STs revealed the presence of new STs as well as the regular presence of ST52 over three decades (1990s–2000s). The proportion of strains that showed resistance to both tiamulin und valnemulin (39.1%) varied considerably among the most frequent STs ranging from 0% (0/14 isolates resistant) in ST8 isolates to 46.7% (14/30), 52.1% (25/48), and 85.7% (6/7) in isolates belonging to ST112, ST52, and ST114, respectively. All hemolysin genes as well as the iron-related gene ftnA and the OMP gene bhlp29.7 were regularly present in the isolates, while the OMP genes bhlp17.6 and bhmp39h could not be detected. Sequence analysis of hemolysin genes of selected isolates revealed co-evolution of tlyB, BHWA1_RS02885, BHWA1_RS09085, and BHWA1_RS02195 with the core genome and suggested independent evolution of tlyA, tlyC, and hlyA. Our data indicate that in Germany, swine dysentery might be caused by a limited number of B. hyodysenteriae clonal groups. Major STs (ST8, ST52, and ST112) are shared with other countries in Europe suggesting a possible role of the European intra-Community trade of pigs in the dissemination of certain clones. The identification of several novel STs, some of which are single or double locus variants of ST52, may on the other hand hint towards an ongoing diversification of the pathogen in the studied area. The linkage of pleuromutilin susceptibility and sequence type of an isolate might reflect a clonal expansion of the underlying resistance mechanism, namely mutations in the ribosomal RNA genes. A linkage between single virulence-associated genes (VAGs) or even VAG patterns and the phylogenetic background of the isolates could not be established, since almost all VAGs were regularly present in the isolates

Herschel far-infrared photometric monitoring of protostars in the Orion Nebula Cluster

We have obtained time series observations of the Orion Nebula Cluster at 70 microns and 160 microns from the Herschel/PACS Photometer. This represents the first wide-field far-infrared photometric monitoring of a young star forming region. The acquired 35'x35' maps show complex extended structures, with unprecedented details, that trace the interaction between the molecular gas and the young hot stars. We detect 43 protostars, most of which are situated along the integral-shaped filament extending from the Orion nebula, through OMC2 and to OMC3. We present high-reliability light curves for some of these objects using the first six epochs of our observing program spread over 6 weeks. We find amplitude variations in excess of 20% for a fraction of the detected protostars over periods as short as a few weeks. This is inconsistent with the dynamical time-scales of cool far-IR emitting material that orbits at hundreds of AU from the protostar, and it suggests that the mechanism(s) responsible for the observed variability originates from the inner region of the protostars, likely driven by variable mass accretion.Comment: 14 pages, 3 figures, 2 table

Canine Brucellosis: Insights Into the Epidemiologic Situation in Europe

Brucella canis is one of many responsible pathogens of discospondylitis in dogs and infections require specific management. Little is known about the epidemiologic situation in Europe. The purpose of the study was to get insights into the occurrence of brucellosis in dogs in Europe. The database of a European veterinary laboratory was screened for Brucella positive samples. Additionally, medical records of a veterinary hospital in Germany were screened for diagnosis of discospondylitis and brucellosis. The laboratory received samples from 20 European countries for Brucella testing in dogs: 3.7% of submitted samples were Brucella spp. PCR-positive (61/1,657), and Brucella canis antibodies were identified in 5.4% of submitted samples (150/2,764). Brucella spp. PCR-positive samples originated from Spain (11.1% of submitted samples), Poland (6.7% of submitted samples) and rarely from Italy and France. Samples with Brucella canis antibodies originated from 13 European countries (Sweden, Belgium, Austria, Switzerland, Italy, Finland, Germany, Denmark, Hungary, Norway, Poland, France, Netherlands). Young dogs (0-24 months) had a 5.4-fold increased risk of PCR positive samples. The supplementary medical records search identified four young female dogs (7-30 months) with Brucella canis discospondylitis in Germany. The four dogs had been imported to Germany from Eastern European countries (Moldavia, Romania, Macedonia). In conclusion, infection with Brucella canis needs to be considered in dogs in Europe and diagnostics for Brucella canis infection appear indicated in young dogs with discospondylitis

Drying of NCM Cathode Electrodes with Porous, Nanostructured Particles Versus Compact Solid Particles: Comparative Study of Binder Migration as a Function of Drying Conditions

Porous, nanostructured Li(NiₓCo$_{y}$Mn)O₂ (NCM) achieves an improvement in the fast-charging capability and the durability of lithium-ion batteries. This improvement is attributed to an extended electrolyte—active material interface, where the electrochemical reactions take place and thus shorter diffusion paths inside the active material particles are necessary for charge transfer. Due to the porous particle morphology, new processing challenges arise compared to compact solid NCM. Herein, the properties of the slurries and the electrodes made of the two active materials and, in particular, the influence of the drying process on the binder distribution, are comparatively investigated. For the same composition of the slurries, a significantly lower dependence of adhesion force and discharge capacity at higher C-rates on the drying rate is shown when using porous, nanostructured particles instead of solid particles. Binder migration and thus an inhomogeneous concentration distribution of the polyvinylidene fluoride binder is less pronounced for these electrodes during faster drying. Cell tests with half cells show that after increasing the drying rate by more than 350%, the discharge capacity of the electrodes consisting of solid NCM is reduced by about 63% at 5C while for the electrodes made of porous material no reduction is measured

In Vitro Antibacterial Activity of Microbial Natural Products against Bacterial Pathogens of Veterinary and Zoonotic Relevance

Antimicrobial resistance (AMR) is considered one of the greatest threats to both human and animal health. Efforts to address AMR include implementing antimicrobial stewardship programs and introducing alternative treatment options. Nevertheless, effective treatment of infectious diseases caused by bacteria will still require the identification and development of new antimicrobial agents. Eight different natural products were tested for antimicrobial activity against seven pathogenic bacterial species (Brachyspira sp., Chlamydia sp., Clostridioides sp., Mannheimia sp., Mycobacterium sp., Mycoplasma sp., Pasteurella sp.). In a first pre-screening, most compounds (five out of eight) inhibited bacterial growth only at high concentrations, but three natural products (celastramycin A [CA], closthioamide [CT], maduranic acid [MA]) displayed activity at concentrations 16 µg/mL against Mannheimia for CA, CT, and MA, respectively. CA, CT, and MA exhibited higher MIC50 and MIC90 values against Pasteurella isolates with a known AMR phenotype against commonly used therapeutic antimicrobial agents than against isolates with unknown AMR profiles. This study demonstrates the importance of whole-cell antibacterial screening of natural products to identify promising scaffolds with broad- or narrow-spectrum antimicrobial activity against important Gram-negative veterinary pathogens with zoonotic potentia

Analysis of variability of TW Hya as observed by MOST and ASAS in 2009

As a continuation of our previous studies in 2007 and 2008, new photometric observations of the T Tauri star TW Hya obtained by the MOST satellite and the ASAS project over 40 days in 2009 with temporal resolution of 0.2 days are presented. A wavelet analysis of the combined MOST-ASAS data provides a rich picture of coherent, intermittent, variable-period oscillations, similarly as discovered in the 2008 data. The periods (1.3 - 10 days) and systematic period shortening on time scales of weeks can be interpreted within the model of magneto-rotationally controlled accretion processes in the inner accretion disk around the star. Within this model and depending on the assumed visibility of plasma parcels causing the oscillations, the observed shortest-period oscillation period may indicate the stellar rotation period of 1.3 or 2.6 d, synchronized with the disk at 4.5 or 7.1 solar radii, respectively.Comment: Accepted to MNRA

Fundamental Flaws of Hormesis for Public Health Decisions

Hormesis (defined operationally as low-dose stimulation, high-dose inhibition) is often used to promote the notion that while high-level exposures to toxic chemicals could be detrimental to human health, low-level exposures would be beneficial. Some proponents claim hormesis is an adaptive, generalizable phenomenon and argue that the default assumption for risk assessments should be that toxic chemicals induce stimulatory (i.e., “beneficial”) effects at low exposures. In many cases, nonmonotonic dose–response curves are called hormetic responses even in the absence of any mechanistic characterization of that response. Use of the term “hormesis,” with its associated descriptors, distracts from the broader and more important questions regarding the frequency and interpretation of nonmonotonic dose responses in biological systems. A better understanding of the biological basis and consequences of nonmonotonic dose–response curves is warranted for evaluating human health risks. The assumption that hormesis is generally adaptive is an oversimplification of complex biological processes. Even if certain low-dose effects were sometimes considered beneficial, this should not influence regulatory decisions to allow increased environmental exposures to toxic and carcinogenic agents, given factors such as interindividual differences in susceptibility and multiplicity in exposures. In this commentary we evaluate the hormesis hypothesis and potential adverse consequences of incorporating low-dose beneficial effects into public health decisions

Photometric variability of the T Tauri star TW Hya on time scales of hours to years

MOST (Microvariability & Oscillations of STars) and ASAS (All Sky Automated Survey) observations have been used to characterize photometric variability of TW Hya on time scales from a fraction of a day to 7.5 weeks and from a few days to 8 years, respectively. The two data sets have very different uncertainties and temporal coverage properties and cannot be directly combined, nevertheless, they suggests a global variability spectrum with "flicker noise" properties, i.e. with amplitudes a ~ 1/sqrt(f), over >4 decades in frequency, in the range f = 0.0003 to 10 cycles per day (c/d). A 3.7 d period is clearly present in the continuous 11 day, 0.07 d time resolution, observations by MOST in 2007. Brightness extrema coincide with zero-velocity crossings in periodic (3.56 d) radial velocity variability detected in contemporaneous spectroscopic observations of Setiawan et al. (2008) and interpreted as caused by a planet. The 3.56/3.7 d periodicity was entirely absent in the second, four times longer MOST run in 2008, casting doubt on the planetary explanation. Instead, a spectrum of unstable single periods within the range of 2 - 9 days was observed; the tendency of the periods to progressively shorten was well traced using the wavelet analysis. The evolving periodicities and the overall flicker-noise characteristics of the TW Hya variability suggest a combination of several mechanisms, with the dominant ones probably related to the accretion processes from the disk around the star.Comment: MNRAS submitte