Prevalence And Clinical Significance Of Potential Drug-drug Interactions In Diabetic Patients Attended In A Tertiary Care Outpatient Center, Brazil

The aim of this study is to investigate the prevalence of potential drug-drug interactions (PDDIs), as well as classifying them in relation to level of severity, scientific evidence, time of onset, and potential clinical impact in adult and older adult patients with diabetes mellitus 2 (DM2). This cross-sectional study was conducted in a tertiary care outpatient center. The consecutive sample was made up of 140 patients with DM2. The Anatomical-Therapeutic-Chemical Classification was used for classifying the classes of medications. The PDDIs were analyzed using the DRUG-REAXA (R) system. The relationships between PDDI and the associated factors were ascertained using a multiple logistic regression model. The prevalence of total PDDI was 75 %, and the prevalence of major severity PDDI was 20.7 %. Simvastatin (30.8 %), captopril/enalapril (12.8 %), and oral anti-diabetics/insulin (12.8 %) were the medications which were most involved in the major PDDI, bringing relevant potential clinical impacts such as rhabdomyolysis, hyperkalemia, and important glycemic alterations. Polypharmacy was associated with PDDI (adjusted odds ratio = 10.46, 95 % confidence interval = 4.10-26.71). Diabetics were highly exposed to clinically significant PDDI. It is important that health professionals should be aware of the risks related to PDDI, so that measures may be implemented in order to assure safe care for the patient.36328328

Robotic Tankette for Intelligent BioEnergy Agriculture: Design, Development and Field Tests

In recent years, the use of robots in agriculture has been increasing mainly due to the high demand of productivity, precision and efficiency, which follow the climate change effects and world population growth. Unlike conventional agriculture, sugarcane farms are usually regions with dense vegetation, gigantic areas, and subjected to extreme weather conditions, such as intense heat, moisture and rain. TIBA - Tankette for Intelligent BioEnergy Agriculture - is the first result of an R&D project which strives to develop an autonomous mobile robotic system for carrying out a number of agricultural tasks in sugarcane fields. The proposed concept consists of a semi-autonomous, low-cost, dust and waterproof tankette-type vehicle, capable of infiltrating dense vegetation in plantation tunnels and carry several sensing systems, in order to perform mapping of hard-to-access areas and collecting samples. This paper presents an overview of the robot mechanical design, the embedded electronics and software architecture, and the construction of a first prototype. Preliminary results obtained in field tests validate the proposed conceptual design and bring about several challenges and potential applications for robot autonomous navigation, as well as to build a new prototype with additional functionality.Comment: 9 pages, 15 figure

Profiling DNA methylation based on next-generation sequencing approaches: New insights and clinical applications

DNA methylation is an epigenetic modification that plays a pivotal role in regulating gene expression and, consequently, influences a wide variety of biological processes and diseases. The advances in next-generation sequencing technologies allow for genome-wide profiling of methyl marks both at a single-nucleotide and at a single-cell resolution. These profiling approaches vary in many aspects, such as DNA input, resolution, coverage, and bioinformatics analysis. Thus, the selection of the most feasible method according with the project’s purpose requires in-depth knowledge of those techniques. Currently, high-throughput sequencing techniques are intensively used in epigenomics profiling, which ultimately aims to find novel biomarkers for detection, diagnosis prognosis, and prediction of response to therapy, as well as to discover new targets for personalized treatments. Here, we present, in brief, a portrayal of next-generation sequencing methodologies’ evolution for profiling DNA methylation, highlighting its potential for translational medicine and presenting significant findings in several diseases.This research was funded by Research Center—Portuguese Oncology Institute of Porto (CI-IPOFB-GEBC-2018 and FCT (POCI-01-0145-FEDER-29043). D.B.-S. is a research fellow from CI-IPOP (BI-GEBC2018/UID/DTP/00776/POCI-01-0145-FEDER-006868), and C.J.M. is a FCT Investigador (FCT contract IF/00047/2012)

Portuguese colonial architectural archives at the Arquivo Histórico Ultramarino, mid 19th century - 1975

The Arquivo Histórico Ultramarino (AHU - Overseas Historical Archive) holds Portuguese architectural and also engineering archival records created and managed by the Colonial Public Offices seated in Lisbon, dating back to the end of the 16th century to 1975. The archives from the offices that performed duties in the Portuguese colonies remained there, even after their independence from Portugal. Until recently, records dated from the mid 19th century onwards were poorly identified, a panorama that changed over the last decade. These records are related to Angola, Cabo Verde, Guinea-Bissao, São Tomé and Príncipe, Mozambique, previous Portugese possessions in India, Macao and East Timor. The AHU hosted two funded projects by the Portuguese Foundation to Science and Technology (FCT) submitted by the Centre for Socioeconomic and Territorial Studies from the Lisbon University Institute (DINÂMIA'CET-IUL). The participation of the AHU in both projects allowed processing these records bulk. The first one was “The Colonial Urbanization Offices”, a three year project which benefited from the collaboration of five archival fellowships during nearly two years. The second project is the “Coast to Coast”, has a similar duration to the previous and will be ongoing until December 2019, with the contribution of an archival fellowship. Two archivists of the AHU participated in these projects as well, supervising the archival processing as researchers.info:eu-repo/semantics/publishedVersio

Effect of boundary conditions on diffusion in two-dimensional granular gases

We analyze the influence of boundary conditions on numerical simulations of the diffusive properties of a two dimensional granular gas. We show in particular that periodic boundary conditions introduce unphysical correlations in time which cause the coefficient of diffusion to be strongly dependent on the system size. On the other hand, in large enough systems with hard walls at the boundaries, diffusion is found to be independent of the system size. We compare the results obtained in this case with Langevin theory for an elastic gas. Good agreement is found. We then calculate the relaxation time and the influence of the mass for a particle of radius $R_s$ in a sea of particles of radius $R_b$. As granular gases are dissipative, we also study the influence of an external random force on the diffusion process in a forced dissipative system. In particular, we analyze differences in the mean square velocity and displacement between the elastic and inelastic cases.Comment: 15 figures eps figures, include

Energy Dissipation and Trapping of Particles Moving on a Rough Surface

We report an experimental, numerical and theoretical study of the motion of a ball on a rough inclined surface. The control parameters are $D$, the diameter of the ball, $\theta$, the inclination angle of the rough surface and $E_{ki}$, the initial kinetic energy. When the angle of inclination is larger than some critical value, $\theta>\theta_{T}$, the ball moves at a constant average velocity which is independent of the initial conditions. For an angle $\theta < \theta_{T}$, the balls are trapped after moving a certain distance. The dependence of the travelled distances on $E_{ki}$, $D$ and $\theta$. is analysed. The existence of two kinds of mechanisms of dissipation is thus brought to light. We find that for high initial velocities the friction force is constant. As the velocity decreases below a certain threshold the friction becomes viscous.Comment: 8 pages RevTeX, 12 Postscript figure

The Steiner Multi Cycle Problem with Applications to a Collaborative Truckload Problem

We introduce a new problem called Steiner Multi Cycle Problem that extends the Steiner Cycle problem in the same way the Steiner Forest extends the Steiner Tree problem. In this problem we are given a complete weighted graph G=(V,E), which respects the triangle inequality, a collection of terminal sets {T_1,..., T_k}, where for each a in [k] we have a subset T_a of V and these terminal sets are pairwise disjoint. The problem is to find a set of disjoint cycles of minimum cost such that for each a in [k], all vertices of T_a belong to a same cycle. Our main interest is in a restricted case where |T_a| = 2, for each a in [k], which models a collaborative less-than-truckload problem with pickup and delivery. In this problem, we have a set of agents where each agent is associated with a set T_a containing a pair of pickup and delivery vertices. This problem arises in the scenario where a company has to periodically exchange goods between two different locations, and different companies can collaborate to create a route that visits all its pairs of locations sharing the total cost of the route. We show that even the restricted problem is NP-Hard, and present some heuristics to solve it. In particular, a constructive heuristic called Refinement Search, which uses geometric properties to determine if agents are close to each other. We performed computational experiments to compare this heuristic to a GRASP based heuristic. The Refinement Search obtained the best solutions in little computational time

Emerging lab-on-a-chip approaches for liquid biopsy in lung cancer: Status in ctcs and ctdna research and clinical validation

Despite the intensive efforts dedicated to cancer diagnosis and treatment, lung cancer (LCa) remains the leading cause of cancer-related mortality, worldwide. The poor survival rate among lung cancer patients commonly results from diagnosis at late-stage, limitations in characterizing tumor heterogeneity and the lack of non-invasive tools for detection of residual disease and early recurrence. Henceforth, research on liquid biopsies has been increasingly devoted to overcoming these major limitations and improving management of LCa patients. Liquid biopsy is an emerging field that has evolved significantly in recent years due its minimally invasive nature and potential to assess various disease biomarkers. Several strategies for characterization of circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) have been developed. With the aim of standardizing diagnostic and follow-up practices, microfluidic devices have been introduced to improve biomarkers isolation efficiency and specificity. Nonetheless, implementation of lab-on-a-chip platforms in clinical practice may face some challenges, considering its recent application to liquid biopsies. In this review, recent advances and strategies for the use of liquid biopsies in LCa management are discussed, focusing on high-throughput microfluidic devices applied for CTCs and ctDNA isolation and detection, current clinical validation studies and potential clinical utility.This work was supported by FEDER funds through COMPETE (POCI-01-0145-FEDER-030831) and by Portuguese funds through Fundação para a Ciência e a Tecnologia (PTDC/BTM-TEC/30831/2017) in the framework of project TRIMARKCHIP. PCCC Beacon projects-2021 (Carmen Jeronimo & Fernando J Monteiro)

Quantitative Proteomic Analysis Reveals Changes in the Benchmark Corynebacterium pseudotuberculosis Biovar Equi Exoproteome after Passage in a Murine Host

Corynebacterium pseudotuberculosis biovar equi is the etiologic agent of ulcerative lymphangitis. To investigate proteins that could be related to the virulence of this pathogen, we combined an experimental passage process using a murine model and high-throughput proteomics with a mass spectrometry, data-independent acquisition (LC-MSE) approach to identify and quantify the proteins released into the supernatants of strain 258_equi. To our knowledge, this approach allowed characterization of the exoproteome of a C. pseudotuberculosis equi strain for the first time. Interestingly, the recovery of this strain from infected mouse spleens induced a change in its virulence potential, and it became more virulent in a second infection challenge. Proteomic screening performed from culture supernatant of the control and recovered conditions revealed 104 proteins that were differentially expressed between the two conditions. In this context, proteomic analysis of the recovered condition detected the induction of proteins involved in bacterial pathogenesis, mainly related to iron uptake. In addition, KEGG enrichment analysis showed that ABC transporters, bacterial secretion systems and protein export pathways were significantly altered in the recovered condition. These findings show that secretion and secreted proteins are key elements in the virulence and adaptation of C. pseudotuberculosis. Collectively, bacterial pathogenesis-related proteins were identified that contribute to the processes of adherence, intracellular growth and evasion of the immune system. Moreover, this study enhances our understanding of the factors that may influence the pathogenesis of C. pseudotuberculosis.Fil: Marques Da Silva, Wanderson. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasil. Institut National de la Recherche Agronomique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carvalho, Rodrigo D. De Oliveira. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Dorella, Fernanda A.. Universidade Federal de Minas Gerais; BrasilFil: Folador, Edson L.. Universidade Federal da Paraíba. Centro de Biotecnologia; BrasilFil: Souza, Gustavo H. M. F.. Waters Corporation; BrasilFil: Pimenta, Adriano M. C.. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; BrasilFil: Figueiredo, Henrique C. P.. Universidade Federal de Minas Gerais; BrasilFil: Le Loir, Yves. Institut National de la Recherche Agronomique; FranciaFil: Silva, Artur. Universidade Federal do Pará; BrasilFil: Azevedo, Vasco. Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas; Brasi

On the velocity distributions of the one-dimensional inelastic gas

We consider the single-particle velocity distribution of a one-dimensional fluid of inelastic particles. Both the freely evolving (cooling) system and the non-equilibrium stationary state obtained in the presence of random forcing are investigated, and special emphasis is paid to the small inelasticity limit. The results are obtained from analytical arguments applied to the Boltzmann equation along with three complementary numerical techniques (Molecular Dynamics, Direct Monte Carlo Simulation Methods and iterative solutions of integro-differential kinetic equations). For the freely cooling fluid, we investigate in detail the scaling properties of the bimodal velocity distribution emerging close to elasticity and calculate the scaling function associated with the distribution function. In the heated steady state, we find that, depending on the inelasticity, the distribution function may display two different stretched exponential tails at large velocities. The inelasticity dependence of the crossover velocity is determined and it is found that the extremely high velocity tail may not be observable at ``experimentally relevant'' inelasticities.Comment: Latex, 14 pages, 12 eps figure