87 research outputs found
Renovation housing: analysis methods and evaluation of investment projects
The article discusses the problem of providing the population of any country affordable and comfortable housing is key in ensuring sustainable economic development. The author has been compiled a statistical analysis of the need for housing renovation in the Tomsk region. The high capital investment projects in this field it seems necessary to look for new sources of funding, as well as pre-assess their effectiveness
GÖÇ VE YAŞAM
Uluslararası Bakalorya Programı, A1 dersi Türk Dili ve Edebiyatı alanında ele alınan bu tezde, Orhan Kemal'in Gurbet Kuşları adlı yapıtında göç olgusu nedenleri ve sonuçlarıyla beraber incelenmiştir. Göç olgusuyla değişen toplumsal yapı, ekonomik ve kültürel farklılıklar çerçevesinde değerlendirilmiştir. Bu tezin amacı, göç olgusunun toplumsal yapıda alt sınıf ve üst sınıflardaki bireyler üzerindeki etkilerini ortaya koymaktır.
Üç ana bölümden oluşan tezin ilk bölümünde yapıta adını veren Gurbet Kuşları kavramı üzerinde durulmuştur. Köylülerin aidiyetsizliği ve uyum sorunu bu bölümde aktarılmıştır. Tezin ikinci bölümünde ise köylülerin köyden kente göç sürecinde yaşadıkları kadın ve erkek figürler üzerinden neden ve sonuçlarıyla işlenmiştir. Tezin üçüncü bölümünde şehirliler başlığı altından genel olarak şehirde – İstanbul – yaşayan insanların göç sürecinde köylülerle yaşadıkları uyumsuzluk ve çatışmalara yer verilmektedir. Çalışmada göç sürecinde şehre yerleşen figürlerin şehirlilerle aralarındaki ekonomik ve kültürel farklılıkların sınıflar arasında geçişe olanak tanımadığı sonucuna varılmıştır
Complete blockage of the mevalonate pathway results in male gametophyte lethality
Plants have two isoprenoid biosynthetic pathways: the cytosolic mevalonate (MVA) pathway and the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Since the discovery of the MEP pathway, possible metabolic cross-talk between these pathways has prompted intense research. Although many studies have shown the existence of such cross-talk using feeding experiments, it remains to be determined if native cross-talk, rather than exogenously applied metabolites, can compensate for complete blockage of the MVA pathway. Previously, Arabidopsis mutants for HMG1 and HMG2 encoding HMG-CoA reductase (HMGR) were isolated. Although it was shown that HMGR1 is a functional HMGR, the enzyme activity of HMGR2 has not been confirmed. It is demonstrated here that HMG2 encodes a functional reductase with similar activity to HMGR1, using enzyme assays and complementation experiments. To estimate the contribution of native cross-talk, an attempt was made to block the MVA pathway by making double mutants lacking both HMG1 and HMG2, but no double homozygotes were detected in the progeny of self-pollinated HMG1/hmg1 hmg2/hmg2 plants. hmg1 hmg2 male gametophytes appeared to be lethal based on crossing experiments, and microscopy indicated that ∼50% of the microspores from the HMG1/hmg1 hmg2/hmg2 plant appeared shrunken and exhibited poorly defined endoplasmic reticulum membranes. In situ hybridization showed that HMG1 transcripts were expressed in both the tapetum and microspores, while HMG2 mRNA appeared only in microspores. It is concluded that native cross-talk from the plastid cannot compensate for complete blockage of the MVA pathway, at least during male gametophyte development, because either HMG1 or HMG2 is required for male gametophyte development
The yield of essential oils in Melaleuca alternifolia (Myrtaceae) is regulated through transcript abundance of genes in the MEP pathway
Medicinal tea tree (Melaleuca alternifolia) leaves contain large amounts of an essential oil, dominated by monoterpenes. Several enzymes of the chloroplastic methylerythritol phosphate (MEP) pathway are hypothesised to act as bottlenecks to the production of monoterpenes. We investigated, whether transcript abundance of genes encoding for enzymes of the MEP pathway were correlated with foliar terpenes in M. alternifolia using a population of 48 individuals that ranged in their oil concentration from 39 -122 mg x g DM(-1). Our study shows that most genes in the MEP pathway are co-regulated and that the expression of multiple genes within the MEP pathway is correlated with oil yield. Using multiple regression analysis, variation in expression of MEP pathway genes explained 87% of variation in foliar monoterpene concentrations. The data also suggest that sesquiterpenes in M. alternifolia are synthesised, at least in part, from isopentenyl pyrophosphate originating from the plastid via the MEP pathway
A plastidial sodium-dependent pyruvate transporter (vol 476, pg 472, 2011)
Furumoto T, Yamaguchi T, Ohshima-Ichie Y, et al. A plastidial sodium-dependent pyruvate transporter (vol 476, pg 472, 2011). Nature. 2011;478(7368):274
The expanded tomato fruit volatile landscape
[EN] The present review aims to synthesize our present knowledge about the mechanisms implied in the biosynthesis of
volatile compounds in the ripe tomato fruit, which have a key role in tomato flavour. The difficulties in identifiying not
only genes or genomic regions but also individual target compounds for plant breeding are addressed. Ample variability
in the levels of almost any volatile compound exists, not only in the populations derived from interspecific crosses
but also in heirloom varieties and even in commercial hybrids. Quantitative trait loci (QTLs) for all tomato aroma volatiles
have been identified in collections derived from both intraspecific and interspecific crosses with different wild
tomato species and they (i) fail to co-localize with structural genes in the volatile biosynthetic pathways and (ii) reveal
very little coincidence in the genomic regions characterized, indicating that there is ample opportunity to reinforce the
levels of the volatiles of interest. Some of the identified genes may be useful as markers or as biotechnological tools
to enhance tomato aroma. Current knowledge about the major volatile biosynthetic pathways in the fruit is summarized.
Finally, and based on recent reports, it is stressed that conjugation to other metabolites such as sugars seems
to play a key role in the modulation of volatile release, at least in some metabolic pathways.We wish to thank the Metabolomics facility at the IBMCP for technical assistance. AG was supported by grants from MinECO and FECYT. This work was facilitated by the European-funded COST action FA1106 QualityFruit.Rambla Nebot, JL.; Tikunov, Y.; Monforte Gilabert, AJ.; Bovy, A.; Granell Richart, A. (2014). The expanded tomato fruit volatile landscape. Journal of Experimental Botany. 65(16):4613-4623. doi:10.1093/jxb/eru128S461346236516Abegaz, E. G., Tandon, K. S., Scott, J. W., Baldwin, E. A., & Shewfelt, R. L. (2004). Partitioning taste from aromatic flavor notes of fresh tomato (Lycopersicon esculentum, Mill) to develop predictive models as a function of volatile and nonvolatile components. Postharvest Biology and Technology, 34(3), 227-235. doi:10.1016/j.postharvbio.2004.05.023Alba, J. M., Montserrat, M., & Fernández-Muñoz, R. (2008). 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Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures
Plant science
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