ALPNAP – A NEW PROJECT ON TRAFFIC-INDUCED NOISE AND AIR POLLUTION ALONG TRANSPORT ROUTES IN THE ALPS

Abstract. ALPNAP (Monitoring and Minimisation of Traffic-Induced Noise and Air Pollution Along Major Alpine Transport Routes) is a new project financed under the EU Interreg III B Alpine Space Programme. It will run from 2005-2007. A network of eleven institutions has been formed with DLR Institute of Atmospheric Physics as the lead partner. The project aims at the integrated use of advanced science-based methods to monitor, assess, and predict air pollution and noise and their impact on the environment, quality of life and health along major transport routes. These methods are adapted to the Alpine topography and its specific meteorological phenomena which often amplify the levels of concentration and noise. The purpose of the project is to promote these methods to regional and local authorities, to supplement standard methods towards more reliable predictions and scenario assessments, to quantify the limits of emissions if given air quality and noise standards are to be met, and to assess the environmental impact of traffic flow changes due to regulations, new infrastructure, or modal shifts. Previous work has shown that the conditions for the propagation of noise and the dispersion of air pollutants in Alpine valleys are quite different, and often considerably more unfavourable than in flat terrain. Standard models which are still applied in such environments, too, are not adequate. The project aims at demonstrating the advantage of more advanced methods taking into account state-of-the-art scientific tools

Die Linke in der SPD

Eine Untersuchung über die Chancen der Linken in der SPD erfordert eigentlich auch eine Einschätzung der Gesamtpartei, die wir hier nicht leisten können. Wrr sprechen von der SPD als einer „Volkspartei" in Anführungszeichen, da mit diesem Begriff die Veränderungen in der SPD seit 1959 nur unzureichend beschrieben werden. Wir gehen davon aus, daß sich spätestens seit 1959 in der SPD eine sozialreformerische Mehrheit durchgesetzt hat, die die bestehende privatkapitalistischeGesellschaft nur noch reformerisch verbessern will. Die Linke iri der SPD sehen wir als Einheit von sicherlich verschiedenen reformistischen Ansätzen. Es geht uns, gerade auch bei den Jusos, nicht um eine Darstellung und Kritik der unterschiedlichen Positionen. Ohne Zweifel bleibt diese Kritik weiterhin notwendig. (Vgl. dazu als ein Beispiel in dieser Zeitschrift: Siegfried Heimann/Bodo Zeuner, Eine neue Integrationsideologie. Zu den Thesen zur Strategie und Taktik des demokratischen Sozialismus des Peter von Oertzen, in: Prokla 14/ 15 (1974), S.105-148).Wir unterscheiden allerdings, ohne das weiter auszuführen, im Spektrum der reformistischen Linken in der SPD sich anpassende Reformisten, die sich mit der Formulierung von Modellen bescheiden und kämpferische Reformisten, die diejenigen, in deren Namen ein „Demokratischer Sozialismus" auf reformistischem Wege erreicht werden soll, als handelnde Subjekte mit einbeziehen

Effects of small-scale turbulence on the growth of two diatoms of different size in a phosphorus-limited medium

15 pages, 7 figures, 5 tables, 1 appendix.-- Issue title: "Workshop on Future Directions in Modelling Physical-Biological Interactions (WKFDPBI)"The effect of turbulence on the nutrient flux towards osmotrophic cells is predicted to be size dependent. This should translate into growth. We experimentally followed and modelled the growth of two marine diatoms of different size (Thalassiosira pseudonana, 6 μm in diameter and Coscinodiscus sp., ca. 109 μm in diameter) under still water and turbulent conditions, using a shaker table. Experiments were done with phosphorus-limited cultures and lasted for ca. 5 days. Turbulence enhanced the growth of Coscinodiscus sp. in agreement with theory but not the growth of T. pseudonana, which was actually slightly lower under turbulence. At the end of the experiments there were about 1.7 times as many Coscinodiscus sp. cells in the turbulent treatment than in the still treatment, while for T. pseudonana almost the same cell concentration was found in both conditions. In addition, the Coscinodiscus sp. cells growing under still conditions presented a higher specific alkaline phosphatase activity than those growing in turbulence which indicates a higher need for phosphorus in the still cultures. A simple dynamic model, based on Michaelis Menten nutrient uptake kinetics, needed nearly no optimisation other than using observed initial conditions of phosphate and cell concentrations. The model showed how an increased nutrient flux towards the cells translates non-linearly into cell growth, most likely by affecting the half-saturation constant (KM). However, since Coscinodiscus sp. experienced significant mortality and cells partially settled to the bottom of the containers, unequivocal support for the size-dependent effect of turbulence on nutrient uptake will require further experiments and more sophisticated modelling. The mechanisms to connect an increased nutrient flux towards cells with population growth and whether this process is size dependent are important in parameterizing the effects of turbulence on marine plankton in coupled physical biological modelsThis work was supported by Spanish Projects TURFI (REN2002-01591/MAR) and VARITEC (CTM2004-04442-C02) and by European Union projects MEDEA (MAS3-CT95-0016) and NTAP (EVK3-CT-2000-00022). This is European Land Ocean Interaction Studies contribution 523/40Peer reviewe

Endosomal accumulation of APP in wobbler motor neurons reflects impaired vesicle trafficking: Implications for human motor neuron disease

Palmisano R, Golfi P, Heimann P, et al. Endosomal accumulation of APP in wobbler motor neurons reflects impaired vesicle trafficking: Implications for human motor neuron disease. BMC Neuroscience. 2011;12(1): 24.Background: The cause of sporadic amyotrophic lateral sclerosis (ALS) is largely unknown but hypotheses about disease mechanisms include oxidative stress, defective axonal transport, mitochondrial dysfunction and disrupted RNA processing. Whereas familial ALS is well represented by transgenic mutant SOD1 mouse models, the mouse mutant wobbler (WR) develops progressive motor neuron degeneration due to a point mutation in the Vps54 gene, and provides an animal model for sporadic ALS. VPS54 protein as a component of a protein complex is involved in vesicular Golgi trafficking; impaired vesicle trafficking might also be mechanistic in the pathogenesis of human ALS. Results: In motor neurons of homozygous symptomatic WR mice, a massive number of endosomal vesicles significantly enlarged (up to 3 mu m in diameter) were subjected to ultrastructural analysis and immunohistochemistry for the endosome-specific small GTPase protein Rab7 and for amyloid precursor protein (APP). Enlarged vesicles were neither detected in heterozygous WR nor in transgenic SOD1(G93A) mice; in WR motor neurons, numerous APP/Rab7-positive vesicles were observed which were mostly LC3-negative, suggesting they are not autophagosomes. Conclusions: We conclude that endosomal APP/Rab7 staining reflects impaired vesicle trafficking in WR mouse motor neurons. Based on these findings human ALS tissues were analysed for APP in enlarged vesicles and were detected in spinal cord motor neurons in six out of fourteen sporadic ALS cases. These enlarged vesicles were not detected in any of the familial ALS cases. Thus our study provides the first evidence for wobbler-like aetiologies in human ALS and suggests that the genes encoding proteins involved in vesicle trafficking should be screened for pathogenic mutations

Absence of Plekhg5 Results in Myelin Infoldings Corresponding to an Impaired Schwann Cell Autophagy, and a Reduced T-Cell Infiltration Into Peripheral Nerves

Lüningschrör P, Slotta C, Heimann P, et al. Absence of Plekhg5 Results in Myelin Infoldings Corresponding to an Impaired Schwann Cell Autophagy, and a Reduced T-Cell Infiltration Into Peripheral Nerves. Frontiers in Cellular Neuroscience. 2020;14: 185.Inflammation and dysregulation of the immune system are hallmarks of several neurodegenerative diseases. An activated immune response is considered to be the cause of myelin breakdown in demyelinating disorders. In the peripheral nervous system (PNS), myelin can be degraded in an autophagy-dependent manner directly by Schwann cells or by macrophages, which are modulated by T-lymphocytes. Here, we show that the NF-κB activator Pleckstrin homology containing family member 5 (Plekhg5) is involved in the regulation of both Schwann cell autophagy and recruitment of T-lymphocytes in peripheral nerves during motoneuron disease. Plekhg5-deficient mice show defective axon/Schwann cell units characterized by myelin infoldings in peripheral nerves. Even at late stages, Plekhg5-deficient mice do not show any signs of demyelination and inflammation. Using RNAseq, we identified a transcriptional signature for an impaired immune response in sciatic nerves, which manifested in a reduced number of CD4+ and CD8+ T-cells. These findings identify Plekhg5 as a promising target to impede myelin breakdown in demyelinating PNS disorders

Primary rat LSECs preserve their characteristic phenotype after cryopreservation

Liver disease is a leading cause of morbidity and mortality worldwide. Recently, the liver non-parenchymal cells have gained increasing attention for their potential role in the development of liver disease. Liver sinusoidal endothelial cells (LSECs), a specialized type of endothelial cells that have unique morphology and function, play a fundamental role in maintaining liver homeostasis. Current protocols for LSEC isolation and cultivation rely on freshly isolated cells which can only be maintained differentiated in culture for a few days. This creates a limitation in the use of LSECs for research and a need for a consistent and reliable source of these cells. To date, no LSEC cryopreservation protocols have been reported that enable LSECs to retain their functional and morphological characteristics upon thawing and culturing. Here, we report a protocol to cryopreserve rat LSECs that, upon thawing, maintain full LSEC-signature features: fenestrations, scavenger receptor expression and endocytic function on par with freshly isolated cells. We have confirmed these features by a combination of biochemical and functional techniques, and super-resolution microscopy. Our findings offer a means to standardize research using LSECs, opening the prospects for designing pharmacological strategies for various liver diseases, and considering LSECs as a therapeutic target