6,073 research outputs found
Expression of complementary RNA from chloroplast transgenes affects editing efficiency of transgene and endogenous chloroplast transcripts
The expression of angiosperm chloroplast genes is modified by C-to-U RNA editing. The mechanism for recognition of the ∼30 C targets of editing is not understood. There is no single consensus sequence surrounding editing sites, though sites can be grouped into small ‘clusters’ of two to five sites exhibiting some sequence similarity. While complementary RNA that guides nucleotides for alteration has been detected in other RNA modification systems, it is not known whether complementary RNA is involved in chloroplast editing site recognition. We investigated the effect of expressing RNA antisense to the sequences −20 to +6 surrounding the RpoB-2 C target of editing, which is a member of a cluster that includes the PsbL-1 and Rps14-1 sites. Previous experiments had shown that chloroplast rpoB transgene transcripts carrying only these 27 nt were edited in vivo at the proper C. Though transcripts carrying sequences −31 to +60 surrounding the RpoB-2 sites were edited in chloroplast transgenic plants, transcripts carrying the −31 to +62 region followed by the 27 nt complementary region were not edited at all. In contrast, a similar construct, in which the C target as well as the preceding and subsequent nucleotides were mismatched within the 27 nt region, was efficiently edited. The presence of any of the four transgenes carrying RpoB-2 sequences in sense and/or antisense orientation resulted in reduced editing at the PsbL-1 site. Chloroplast transgenic plants expressing the three different antisense RNA constructs exhibited abnormal growth and development, though plants expressing the 92 nt sense transcripts were phenotypically normal
Integrated Analysis of Production Potential and Profitability of a Horizontal Well in the Lower Glen Rose Formation, Maverick County, Texas
The U.S. Department of Energy/Morgantown Energy Technology Center (DOE/METC) awarded a contract in 1991 to Prime Energy Corporation (PEC) to demonstrate the benefit of using horizontal wells to recover gas from low permeability formations. The project area was located in the Chittim field of Maverick County, Texas. The Lower Glen Rose Formation in the Chittim field was a promising horizontal well candidate based on the heterogenous nature of the reservoir (suggested by large well-to-well variances in reserves) and the low percentage of economical vertical wells. Since there was substantial evidence of reservoir heterogeneity, it was unknown whether the selected, wellsite would penetrate a reservoir with the desired properties for a horizontal well. Thus, an integrated team was formed to combine geologic analysis, seismic interpretation, reservoir engineering, reservoir simulation, and economic assessment to analyze the production potential and profitability of completing a horizontal well in the Lower Glen Rose formation
Impact of Electronic Chronic Pain Questions on patient-reported outcomes and healthcare utilization, and attitudes toward eCPQ use among patients and physicians: prospective pragmatic study in a US general practice setting
OBJECTIVE: The Electronic Chronic Pain Questions (eCPQ) has been developed to help healthcare providers systematically capture chronic pain data. This study evaluated the impact of using the eCPQ on patient-reported outcomes (PROs) and healthcare resource utilization (HCRU) in a primary care setting, and patient and physician perceptions regarding use of, and satisfaction with, the eCPQ.
METHODS: This was a prospective pragmatic study conducted at the Internal Medicine clinic within the Henry Ford Health (HFH) Detroit campus between June 2017 and April 2020. Patients (aged ≥18 years) attending the clinic for chronic pain were allocated to an Intervention Group to complete the eCPQ in addition to regular care, or a control group to receive regular care only. The Patient Health Questionnaire-2 and a Patient Global Assessment were assessed at baseline, 6-months, and 12-months study visits. HCRU data were extracted from the HFH database. Telephone qualitative interviews were conducted with randomly selected patients and physicians who used the eCPQ.
RESULTS: Two hundred patients were enrolled, 79 in each treatment group completed all 3 study visits. No significant differences (p \u3e 0.05) were found in PROs and HCRU between the 2 groups. In qualitative interviews, physicians and patients reported the eCPQ as useful, and using the eCPQ improved patient-clinician interactions.
CONCLUSION: Adding the eCPQ to regular care for patients with chronic pain did not significantly impact the PROs assessed in this study. However, qualitative interviews suggested that the eCPQ was a well-accepted and potentially useful tool from a patient and physician perspective. By using the eCPQ, patients were better prepared when they attended a primary care visit for their chronic pain and the quality of patient-physician communication was increased
Design, Implementation and First Measurements with the Medipix Neutron Camera in CMS
The Medipix detector is the first device dedicated to measuring mixed-field
radiation in the CMS cavern and able to distinguish between different particle
types. Medipix2-MXR chips bump bonded to silicon sensors with various neutron
conversion layers developed by the IEAP CTU in Prague were successfully
installed for the 2008 LHC start-up in the CMS experimental and services
caverns to measure the flux of various particle types, in particular neutrons.
They have operated almost continuously during the 2010 run period, and the
results shown here are from the proton run between the beginning of July and
the end of October 2010. Clear signals are seen and different particle types
have been observed during regular LHC luminosity running, and an agreement in
the measured flux rate is found with the simulations. These initial results are
promising, and indicate that these devices have the potential for further and
future LHC and high energy physics applications as radiation monitoring devices
for mixed field environments, including neutron flux monitoring. Further
extensions are foreseen in the near future to increase the performance of the
detector and its coverage for monitoring in CMS.Comment: 15 pages, 16 figures, submitted to JINS
Search for scalar leptoquarks and T-odd quarks in the acoplanar jet topology using 2.5 fb-1 of ppbar collision data at sqrt(s)=1.96 TeV
A search for new physics in the acoplanar jet topology has been performed in
2.5 fb-1 of data from ppbar collisions at sqrt(s)=1.96 TeV, recorded by the D0
detector at the Fermilab Tevatron Collider. The numbers of events with exactly
two acoplanar jets and missing transverse energy are in good agreement with the
standard model expectations. The result of this search has been used to set a
lower mass limit of 205 GeV at the 95% C.L. on the mass of a scalar leptoquark
when this particle decays exclusively into a quark and a neutrino. In the
framework of the Little Higgs model with T-parity, limits have also been
obtained on the T-odd quark mass as a function of the T-odd photon mass
Measurement of the B0_s semileptonic branching ratio to an orbitally excited D_s** state, Br(B0_s -> Ds1(2536) mu nu)
In a data sample of approximately 1.3 fb-1 collected with the D0 detector
between 2002 and 2006, the orbitally excited charm state D_s1(2536) has been
observed with a measured mass of 2535.7 +/- 0.6 (stat) +/- 0.5 (syst) MeV via
the decay mode B0_s -> D_s1(2536) mu nu X. A first measurement is made of the
branching ratio product Br(b(bar) -> D_s1(2536) mu nu X).Br(D_s1(2536)->D*
K0_S). Assuming that D_s1(2536) production in semileptonic decay is entirely
from B0_s, an extraction of the semileptonic branching ratio Br(B0_s ->
D_s1(2536) mu nu X) is made.Comment: 7 pages, 2 figures, LaTeX, version with minor changes as accepted by
Phys. Rev. Let
Simultaneous measurement of the ratio B(t->Wb)/B(t->Wq) and the top quark pair production cross section with the D0 detector at sqrt(s)=1.96 TeV
We present the first simultaneous measurement of the ratio of branching
fractions, R=B(t->Wb)/B(t->Wq), with q being a d, s, or b quark, and the top
quark pair production cross section sigma_ttbar in the lepton plus jets channel
using 0.9 fb-1 of ppbar collision data at sqrt(s)=1.96 TeV collected with the
D0 detector. We extract R and sigma_ttbar by analyzing samples of events with
0, 1 and >= 2 identified b jets. We measure R = 0.97 +0.09-0.08 (stat+syst) and
sigma_ttbar = 8.18 +0.90-0.84 (stat+syst)} +/-0.50 (lumi) pb, in agreement with
the standard model prediction.Comment: submitted to Phys.Rev.Letter
Search for R-parity violating supersymmetry via the LLE couplings lambda_{121}, lambda_{122} or lambda_{133} in ppbar collisions at sqrt(s)=1.96 TeV
A search for gaugino pair production with a trilepton signature in the
framework of R-parity violating supersymmetry via the couplings lambda_121,
lambda_122, or lambda_133 is presented. The data, corresponding to an
integrated luminosity of L~360/pb, were collected from April 2002 to August
2004 with the D0 detector at the Fermilab Tevatron Collider, at a
center-of-mass energy of sqrt(s)=1.96 TeV. This analysis considers final states
with three charged leptons with the flavor combinations eel, mumul, and eetau
(l=e or mu). No evidence for supersymmetry is found and limits at the 95%
confidence level are set on the gaugino pair production cross section and lower
bounds on the masses of the lightest neutralino and chargino are derived in two
supersymmetric models.Comment: 9 pages, 4 figures (fig2 includes 3 subfigures
Measurements of differential cross sections of Z/gamma*+jets+X events in proton anti-proton collisions at sqrt{s}=1.96 TeV
We present cross section measurements for Z/gamma*+jets+X production,
differential in the transverse momenta of the three leading jets. The data
sample was collected with the D0 detector at the Fermilab Tevatron proton
anti-proton collider at a center-of-mass energy of 1.96 TeV and corresponds to
an integrated luminosity of 1 fb-1. Leading and next-to-leading order
perturbative QCD predictions are compared with the measurements, and agreement
is found within the theoretical and experimental uncertainties. We also make
comparisons with the predictions of four event generators. Two
parton-shower-based generators show significant shape and normalization
differences with respect to the data. In contrast, two generators combining
tree-level matrix elements with a parton shower give a reasonable description
of the the shapes observed in data, but the predicted normalizations show
significant differences with respect to the data, reflecting large scale
uncertainties. For specific choices of scales, the normalizations for either
generator can be made to agree with the measurements.Comment: Published in PLB. 11 pages, 3 figure
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