8,467 research outputs found
DNA-Protein Binding Rates: Bending Fluctuation and Hydrodynamic Coupling Effects
We investigate diffusion-limited reactions between a diffusing particle and a
target site on a semiflexible polymer, a key factor determining the kinetics of
DNA-protein binding and polymerization of cytoskeletal filaments. Our theory
focuses on two competing effects: polymer shape fluctuations, which speed up
association, and the hydrodynamic coupling between the diffusing particle and
the chain, which slows down association. Polymer bending fluctuations are
described using a mean field dynamical theory, while the hydrodynamic coupling
between polymer and particle is incorporated through a simple heuristic
approximation. Both of these we validate through comparison with Brownian
dynamics simulations. Neither of the effects has been fully considered before
in the biophysical context, and we show they are necessary to form accurate
estimates of reaction processes. The association rate depends on the stiffness
of the polymer and the particle size, exhibiting a maximum for intermediate
persistence length and a minimum for intermediate particle radius. In the
parameter range relevant to DNA-protein binding, the rate increase is up to
100% compared to the Smoluchowski result for simple center-of-mass motion. The
quantitative predictions made by the theory can be tested experimentally.Comment: 21 pages, 11 figures, 1 tabl
Substrate concentration dependence of the diffusion-controlled steady-state rate constant
The Smoluchowski approach to diffusion-controlled reactions is generalized to
interacting substrate particles by including the osmotic pressure and
hydrodynamic interactions of the nonideal particles in the Smoluchoswki
equation within a local-density approximation. By solving the strictly
linearized equation for the time-independent case with absorbing boundary
conditions, we present an analytic expression for the diffusion-limited
steady-state rate constant for small substrate concentrations in terms of an
effective second virial coefficient B_2*. Comparisons to Brownian dynamics
simulations excluding HI show excellent agreement up to bulk number densities
of B_2* rho_0 < 0.4 for hard sphere and repulsive Yukawa-like interactions
between the substrates. Our study provides an alternative way to determine the
second virial coefficient of interacting macromolecules experimentally by
measuring their steady-state rate constant in diffusion-controlled reactions at
low densities.Comment: 7 pages, 3 figure
Shear-induced reaction-limited aggregation kinetics of Brownian particles at arbitrary concentrations
The aggregation of interacting Brownian particles in sheared concentrated
suspensions is an important issue in colloid and soft matter science per se.
Also, it serves as a model to understand biochemical reactions occurring in
vivo where both crowding and shear play an important role. We present an
effective medium approach within the Smoluchowski equation with shear which
allows one to calculate the encounter kinetics through a potential barrier
under shear at arbitrary colloid concentrations. Experiments on a model
colloidal system in simple shear flow support the validity of the model in the
range considered. By generalizing Kramers' rate theory to the presence of
collective hydrodynamics, our model explains the significant increase in the
shear-induced reaction-limited aggregation kinetics upon increasing the colloid
concentration
To swim or not to swim: an interpretation of farmed mink's motivation for a water bath
How an animalâs behavioural (ethological) needs can be met is a pivotal issue in the assessment of welfare for captive animals. The value of swimming water for farmed mink is an example how scientific and societal questions relating to animal welfare can be answered. A number of studies have addressed the issue of the indispensability of swimming water for mink; however, so far with inconclusive evidence. In this paper, the results of these studies and related literature are reviewed.
First, the biological definition of need is discussed. Subsequently, attention is paid to the effects of the presence, absence and the removal of swimming water on behavioural and physiological correlates of well-being including stereotypic and anticipatory behaviour and urinary cortisol. Thereafter we discuss individual differences in the use of swimming water, the price animals pay for access to a water bath, and the effect of access to swimming water on juvenile play.
The main conclusions of the literature review are that 1) the use of a water bath for mink is most likely related to foraging behaviour (foraging areas: land and water); 2) absence of swimming water, without prior experience, does not lead to consistent changes in level of stereotypic behaviour, or anticipatory responses; 3) removal of a previously experienced water bath may induce short-term stress as indicated by behavioural parameters and elevated cortisol responses; 4) mink work hard for access to a swimming bath and running wheel in consumer demand studies. Other cage modifications such as tunnels and biting objects, may also provide environmental enrichment, if they are added to otherwise impoverished conditions; 5) There are individual differences in the use of swimming water: these are related in part to variation in prior experience of aquatic resources.; 6) As prior experience is important both with respect to individual use of swimming water and the response to deprivation, swimming water can not be described as biological need in the sense of a fixed requirement for survival. As swimming water appears to act as an incentive that induces its own motivation a more accurate term may be an âincentive induced or environmentally facilitated needâ. Given the available evidence, it is not possible to conclude whether mink that have never experienced swimming water, suffer as a consequence of its absence. However, it is possible to predict that mink with access to water have improved quality of life, due to increased behavioural opportunities, in comparison to farmed mink without access to swimming water. In practical terms, it is still open to debate whether mink should be provided with swimming water, or if alternative, less valued, but easier to install and maintain forms of environmental enrichment, should be provided in mink housing.
To clarify these issues a number of future studies would be valuable. These include; 1) whether specific environmental cues affect motivation to swim, such as the form of drinking water delivery systems ; 2) whether prior experience of swimming water affects its incentive value; in other words âcan you miss what you never experienced?â; 3) do behavioural parameters such as stereotypic behaviour; rebound effects and vacuum activity have any general utility in assessing the value of absent resources; 4) what are preferences for and the value of alternative resources which may act as substitutes for swimming water. In addition we would recommend further work investigating: relationship between access to swimming water and positive indicators of welfare such as play and/or anticipatory behaviour; the effects of preventing the performance of rewarding behaviours and deprivation of a previous experienced resource; and health and hygeine issues related to provision of a water bath. In future work, it would be desirable to present be the actual percentages of animals using a water bath during the experiment and the use of power analyses, to aid their interpretation
A large stellar evolution database for population synthesis studies: VI. White dwarf cooling sequences
We present a new set of cooling models and isochrones for both H- and
He-atmosphere white dwarfs, incorporating accurate boundary conditions from
detailed model atmosphere calculations, and carbon-oxygen chemical abundance
profiles based on updated stellar evolution calculations from the BaSTI stellar
evolution archive - a theoretical data center for the Virtual Observatory. We
discuss and quantify the uncertainties in the cooling times predicted by the
models, arising from the treatment of mixing during the central H- and
He-burning phases, number of thermal pulses experienced by the progenitors,
progenitor metallicity and the reaction rate. The
largest sources of uncertainty turn out to be related to the treatment of
convection during the last stages of the progenitor central He-burning phase,
and the reaction rate. We compare our new models
to previous calculations performed with the same stellar evolution code, and
discuss their application to the estimate of the age of the solar neighborhood,
and the interpretation of the observed number ratios between H- and
He-atmosphere white dwarfs. The new white dwarf sequences and an extensive set
of white dwarf isochrones that cover a large range of ages and progenitor
metallicities are made publicly available at the official BaSTI website.Comment: 34 pages, 13 figures, The Astrophysical Journal, in pres
The white dwarf cooling sequence in the old open cluster NGC 188
We develop the white dwarf luminosity function (LF) of the old open cluster
NGC 188 in order to determine a lower limit to the age of the cluster by using
the faint end of the cooling sequence.
To produce an extensive sequence of the cooling white dwarfs we imaged four
contiguous HST-WFPC2 fields in the center of the cluster in the F555W and F814W
filters. After imposing selection criteria on the detected objects we found a
white dwarf cooling sequence (down to V ~26.5) including 28 candidate white
dwarfs in the cluster. The exposures are not deep enough to reach the end of
this sequence, but the results of our analysis allow us to establish a lower
limit to the age of the cluster independently of the isochrone fit to the
cluster turnoff.
The most ancient white dwarfs found are ~4 Gyr old, an age that is set solely
by the photometric limit of our data. Classical methods provide an estimate of
\~7 Gyr (Sarajedini et al., 1999).Comment: 6 pages, 5 figures, accepted on Astronomy and Astrophysic
Kramers rate theory of ionization and dissociation of bound states
Calculating the microscopic dissociation rate of a bound state, such as a
classical diatomic molecule, has been difficult so far. The problem was that
standard theories require an energy barrier over which the bound particle (or
state) escapes into the preferred low-energy state. This is not the case when
the long-range repulsion responsible for the barrier is either absent or
screened (as in Cooper pairs, ionized plasma, or biomolecular complexes). We
solve this classical problem by accounting for entropic memory at the
microscopic level. The theory predicts dissociation rates for arbitrary
potentials and is successfully tested on the example of plasma, where it yields
an estimate of ionization in the core of Sun in excellent agreement with
experiments. In biology, the new theory accounts for crowding in
receptor-ligand kinetics and protein aggregation
Prediction of the Atomization Energy of Molecules Using Coulomb Matrix and Atomic Composition in a Bayesian Regularized Neural Networks
Exact calculation of electronic properties of molecules is a fundamental step
for intelligent and rational compounds and materials design. The intrinsically
graph-like and non-vectorial nature of molecular data generates a unique and
challenging machine learning problem. In this paper we embrace a learning from
scratch approach where the quantum mechanical electronic properties of
molecules are predicted directly from the raw molecular geometry, similar to
some recent works. But, unlike these previous endeavors, our study suggests a
benefit from combining molecular geometry embedded in the Coulomb matrix with
the atomic composition of molecules. Using the new combined features in a
Bayesian regularized neural networks, our results improve well-known results
from the literature on the QM7 dataset from a mean absolute error of 3.51
kcal/mol down to 3.0 kcal/mol.Comment: Under review ICANN 201
Outer boundary conditions for evolving cool white dwarfs
White dwarf evolution is essentially a gravothermal cooling process,
which,for cool white dwarfs, sensitively depends on the treatment of the outer
boundary conditions. We provide detailed outer boundary conditions appropriate
for computing the evolution of cool white dwarfs employing detailed non-gray
model atmospheres for pure H composition. We also explore the impact on the
white dwarf cooling times of different assumptions for energy transfer in the
atmosphere of cool white dwarfs. Detailed non-gray model atmospheres are
computed taken into account non-ideal effects in the gas equation of state and
chemical equilibrium, collision-induced absorption from molecules, and the
Lyman alpha quasi-molecular opacity. Our results show that the use of detailed
outer boundary conditions becomes relevant for effective temperatures lower
than 5800 and 6100K for sequences with 0.60 and 0.90 M_sun, respectively.
Detailed model atmospheres predict ages that are up to approx 10% shorter at
log L/L_sun=-4 when compared with the ages derived using Eddington-like
approximations at tau_Ross=2/3. We also analyze the effects of various
assumptions and physical processes of relevance in the calculation of outer
boundary conditions. In particular, we find that the Ly_alpha red wing
absorption does not affect substantially the evolution of white dwarfs. White
dwarf cooling timescales are sensitive to the surface boundary conditions for
T_eff < 6000K. Interestingly enough, non-gray effects have little consequences
on these cooling times at observable luminosities. In fact, collision-induced
absorption processes, which significantly affect the spectra and colors of old
white dwarfs with hydrogen-rich atmospheres, have not noticeable effects in
their cooling rates, except throughout the Rosseland mean opacity.Comment: 6 pages, 9 figures, to be published in Astronomy and Astrophysic
The Spectral Energy Distributions of White Dwarfs in 47 Tucanae: The Distance to the Cluster
We present a new distance determination to the Galactic globular cluster 47
Tucanae by fitting the spectral energy distributions of its white dwarfs to
pure hydrogen atmosphere white dwarf models. Our photometric dataset is
obtained from a 121 orbit Hubble Space Telescope program using the Wide Field
Camera 3 UVIS/IR channels, capturing F390W, F606W, F110W, and F160W images.
These images cover more than 60 square arcmins and extend over a radial range
of 5-13.7 arcmin (6.5-17.9 pc) within the globular cluster. Using a likelihood
analysis, we obtain a best fitting unreddened distance modulus of (m -
M)o=13.36+/-0.02+/-0.06 corresponding to a distance of 4.70+/-0.04+/-0.13 kpc,
where the first error is random and the second is systematic. We also search
the white dwarf photometry for infrared excess in the F160W filter, indicative
of debris disks or low mass companions, and find no convincing cases within our
sample.Comment: Accepted to The Astronomical Journal, 13 Figures, 2 Tables. Figures 3
and 6 are figure sets, each composed of 59 subfigures (to appear in the
electronic journal). This is a Companion paper to the article ID:
submit/037561
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