Die Deutschen und ihr Verhältnis zu ihrem höchsten Gericht : eine »Quasifestschrift« der besonderen Art: In dem Sammelband zum 60. Geburtstag des Bundesverfassungsgerichts kommen Wissenschaftler verschiedener Disziplinen zu Wort

Rezension zu: Michael Stolleis (Hrsg.) Herzkammern der Republik. Die Deutschen und das Bundesverfassungsgericht. München 2011, Verlag C.H. Beck, ISBN 978-3-406-62377-6, 298 Seiten, 20,95 Euro

Freiheit der Literatur und Verfassungsrecht : Kollisionen zwischen der Kunstfreiheit und dem allgemeinen Persönlichkeitsrecht

Das Verhältnis zwischen Literatur und Verfassungsrecht, zwischen Produzenten und Vermittlern literarischer Kunst einerseits und dem Bundesverfassungsgericht andererseits, ist schwierig. Verantwortlich dafür sind nicht die Beteiligten, sondern grundlegende systemische Differenzen. Wo literarische Kunst Rätsel aufgibt oder aufgeben darf, muss das Recht Rätsel ausschließen, den Zweifel zum Schweigen bringen. Wo die Literatur mit Mehrdeutigkeit spielen kann, ihre Interpretation in permanenter Fluktuation begriffen, unerschöpflich und deshalb auch unabschließbar ist, müssen Gerichte am Ende eines Verfahrens systembedingt unausweichlich zu einer Entscheidung gelangen

Evaluation of the shortwave cloud radiative effect over the ocean by use of ship and satellite observations

In this study the shortwave cloud radiative effect (SWCRE) over ocean calculated by the ECHAM 5 climate model is evaluated for the cloud property input derived from ship based measurements and satellite based estimates and compared to ship based radiation measurements. The ship observations yield cloud fraction, liquid water path from a microwave radiometer, cloud bottom height as well as temperature and humidity profiles from radiosonde ascents. Level-2 products of the Satellite Application Facility on Climate Monitoring (CM~SAF) from the Spinning Enhanced Visible and InfraRed Imager (SEVIRI) have been used to characterize clouds. Within a closure study six different experiments have been defined to find the optimal set of measurements to calculate downward shortwave radiation (DSR) and the SWCRE from the model, and their results have been evaluated under seven different synoptic situations. Four of these experiments are defined to investigate the advantage of including the satellite-based cloud droplet effective radius as additional cloud property. The modeled SWCRE based on satellite retrieved cloud properties has a comparable accuracy to the modeled SWCRE based on ship data. For several cases, an improvement through introducing the satellite-based estimate of effective radius as additional information to the ship based data was found. Due to their different measuring characteristics, however, each dataset shows best results for different atmospheric conditions

Collaborative study to evaluate a candidate World Health Organization international standard for chikungunya virus for nucleic acid amplification technique (NAT)-based assays

Collaborative Study Group - Portugal:Maria João Alves, Líbia Zé-Zé (National Institute of Health Dr. Ricardo Jorge, Center for Vectors and Infectious Diseases Research)This report describes the World Health Organization (WHO) project to develop an international standard (IS) for Chikungunya virus (CHIKV) RNA for use with nucleic acid amplification technique (NAT)-based assays. An international collaborative study was conducted to determine the potency of the candidate standard using a range of NAT-based assays for CHIKV, and to evaluate the suitability of the candidate for the calibration of secondary reference materials and the standardization of CHIKV viral load measurements. The candidate standard consisted of a heat inactivated CHIKV strain of the East/South/Central African genotype (ESCA), also known as the Indian Ocean Lineage, isolated from a patient returning from India to the United States in 20061 , diluted in human negative plasma. The lyophilized candidate preparation (Sample 1), the corresponding liquid-frozen bulk material (Sample 2) and three different clinical samples (Sample 3, Sample 4 and Sample 5) were included in the collaborative study. Twenty-five laboratories representing 14 countries participated in the study to evaluate the material using their routine CHIKV NAT assays. Twenty-four laboratories returned 31 data sets from 17 commercial assays and 14 in-house methods. Of these 31 methods, 11 were quantitative and 20 were qualitative. The results of the study indicate the suitability of the candidate material of the CHIKV strain of ESCA genotype (Sample 1) as the proposed 1st WHO IS for CHIKV. It is therefore proposed that the candidate material (PEI code 11785/16) is established as the 1st WHO IS for CHIKV RNA for NAT-based assays with an assigned potency of 2,500,000 International Units (IU)/mL when reconstituted in 0.5 mL of nuclease-free water. On-going studies for real-time and accelerated stability of the proposed IS indicate that the preparation is stable and suitable for long-term use under the proposed storage conditions.info:eu-repo/semantics/publishedVersio

Redox regulation of inflammatory processes is enzymatically controlled

Redox regulation depends on the enzymatically controlled production and decay of redox active molecules. NADPH oxidases, superoxide dismutases, nitric oxide synthases, and others produce the redox active molecules superoxide, hydrogen peroxide, nitric oxide, and hydrogen sulfide. These react with target proteins inducing spatiotemporal modifications of cysteine residues within different signaling cascades. Thioredoxin family proteins are key regulators of the redox state of proteins. They regulate the formation and removal of oxidative modifications by specific thiol reduction and oxidation. All of these redox enzymes affect inflammatory processes and the innate and adaptive immune response. Interestingly, this regulation involves different mechanisms in different biological compartments and specialized cell types. The localization and activity of distinct proteins including, for instance, the transcription factor NFκB and the immune mediator HMGB1 are redox-regulated. The transmembrane protein ADAM17 releases proinflammatory mediators, such as TNFα, and is itself regulated by a thiol switch. Moreover, extracellular redox enzymes were shown to modulate the activity and migration behavior of various types of immune cells by acting as cytokines and/or chemokines. Within this review article, we will address the concept of redox signaling and the functions of both redox enzymes and redox active molecules in innate and adaptive immune responses

Impact of AVHRR channel 3b noise on climate data records: filtering method applied to the CM SAF CLARA-A2 data record

A method for reducing the impact of noise in the 3.7 micron spectral channel in climate data records derived from coarse resolution (4 km) global measurements from the Advanced Very High Resolution Radiometer (AVHRR) data is presented. A dynamic size-varying median filter is applied to measurements guided by measured noise levels and scene temperatures for individual AVHRR sensors on historic National Oceanic and Atmospheric Administration (NOAA) polar orbiting satellites in the period 1982–2001. The method was used in the preparation of the CM SAF cLoud, Albedo and surface RAdiation dataset from AVHRR data—Second Edition (CLARA-A2), a cloud climate data record produced by the EUMETSAT Satellite Application Facility for Climate Monitoring (CM SAF), as well as in the preparation of the corresponding AVHRR-based datasets produced by the European Space Agency (ESA) Climate Change Initiative (CCI) project ESA-CLOUD-CCI. The impact of the noise filter was equivalent to removing an artificial decreasing trend in global cloud cover of 1–2% per decade in the studied period, mainly explained by the very high noise levels experienced in data from the first satellites in the series (NOAA-7 and NOAA-9). View Full-Tex

Thioredoxin family proteins in physiology and disease

Proteins of the thioredoxin (Trx) family are ubiquitously expressed oxidoreductases. They use cysteinyl residues within their active site to modify substrate proteins posttranslationally by reduction/oxidation reactions or de-/glutathionylation. They play a crucial role in regulating cellular functions such as proliferation, differentiation and apoptosis. This thesis entitled “Thioredoxin family proteins in physiology and disease” focusses on these proteins, i.e. the Trx systems, the glutaredoxin (Grx) systems and the peroxiredoxins (Prxs). Because descriptions of cellular functions of redoxins are rare, we aimed at identifying new interaction partners and functions under physiological and hypoxic conditions in various cell culture and animal models. This thesis emphasizes the concept of compartmentalised redox signaling and demonstrates not only the complexity of the Trx family proteins, but the species-, tissue- and cell type-specific responses to oxygen deprivation and the distinct contribution of the redoxins to controling the fate of a cell. In detail: - Human, mitochondrial Grx2 is an electron donor for Prx3. - Vertebrate Grx3 (human and zebrafish) is involved in iron regulation. - Trx family proteins showed a tissue- and cell type-specific expression and distribution in the rat CNS and responded tissue- and cell type-specific to oxygen deprivation in numerous cell lines and models for perinatal asphyxia, renal ischemia/reperfusion (I/R) injury and transplantation of pancreatic β-cells. (compare to summary of the thesis

Redox proteomics of the inflammatory secretome identifies a common set of redoxins and other glutathionylated proteins released in inflammation, influenza virus infection and oxidative stress

Protein cysteines can form transient disulfides with glutathione (GSH), resulting in the production of glutathionylated proteins, and this process is regarded as a mechanism by which the redox state of the cell can regulate protein function. Most studies on redox regulation of immunity have focused on intracellular proteins. In this study we have used redox proteomics to identify those proteins released in glutathionylated form by macrophages stimulated with lipopolysaccharide (LPS) after pre-loading the cells with biotinylated GSH. Of the several proteins identified in the redox secretome, we have selected a number for validation. Proteomic analysis indicated that LPS stimulated the release of peroxiredoxin (PRDX) 1, PRDX2, vimentin (VIM), profilin1 (PFN1) and thioredoxin 1 (TXN1). For PRDX1 and TXN1, we were able to confirm that the released protein is glutathionylated. PRDX1, PRDX2 and TXN1 were also released by the human pulmonary epithelial cell line, A549, infected with influenza virus. The release of the proteins identified was inhibited by the anti-inflammatory glucocorticoid, dexamethasone (DEX), which also inhibited tumor necrosis factor (TNF)-α release, and by thiol antioxidants (N-butanoyl GSH derivative, GSH-C4, and N-acetylcysteine (NAC), which did not affect TNF-α production. The proteins identified could be useful as biomarkers of oxidative stress associated with inflammation, and further studies will be required to investigate if the extracellular forms of these proteins has immunoregulatory functions

Cysteine oxidation targets peroxiredoxins 1 and 2 for exosomal release through a novel mechanism of redox-dependent secretion

Non-classical protein secretion is of major importance as a number of cytokines and inflammatory mediators are secreted via this route. Current evidence indicates that there are several mechanistically distinct methods of non-classical secretion. We have recently shown that peroxiredoxin (Prdx) 1 and Prdx2 are released by various cells upon exposure to inflammatory stimuli such as LPS or TNF-α. The released Prdx then acts to induce production of inflammatory cytokines. However, Prdx1 and 2 do not have signal peptides and therefore must be secreted by alternative mechanisms as has been postulated for the inflammatory mediators IL-1β and HMGB1. We show here that circulating Prdx1 and 2 are present exclusively as disulphide-linked homodimers. Inflammatory stimuli also induce in vitro release of Prdx1 and 2 as disulfide-linked homodimers. Mutation of cysteines Cys51 or Cys172 (but not Cys70) in Prdx2, and Cys52 or Cys173 (but not Cys71 or Cys83) in Prdx1 prevented dimer formation and this was associated with inhibition of their TNF-α-induced release. Thus, the presence and oxidation of key cysteine residues in these proteins are a prerequisite for their secretion in response to TNF-α and this release can be induced with an oxidant. In contrast, the secretion of the nuclear-associated danger signal HMGB1 is independent of cysteine oxidation, as shown by experiments with a cysteine-free HMGB1 mutant. Release of Prdx1 and 2 is not prevented by inhibitors of the classical secretory pathway; instead, both Prdx1 and 2 are released in exosomes from both HEK cells and monocytic cells. Serum Prdx1 and 2 are also associated with the exosomes. These results describe a novel pathway of protein secretion mediated by cysteine oxidation that underlines the importance of redox-dependent signalling mechanisms in inflammation