Extreme internal charging currents in medium Earth orbit: Analysis of SURF plate currents on Giove-A

Relativistic electrons can penetrate spacecraft shielding and can damage satellite components. Spacecraft in medium Earth orbit pass through the heart of the outer radiation belt and may be exposed to large fluxes of relativistic electrons, particularly during extreme space weather events. In this study we perform an extreme value analysis of the daily average internal charging currents at three different shielding depths in medium Earth orbit as a function of L∗ and along the orbit path. We use data from the SURF instrument on board the European Space Agency's Giove-A spacecraft from December 2005 to January 2016. The top, middle, and bottom plates of this instrument respond to electrons with energies >500 keV, >700 keV, and >1.1 MeV, respectively. The 1 in 10 year daily average top plate current decreases with increasing L∗ ranging from 1.0 pA cm−2 at L∗=4.75 to 0.03 pA cm−2 at L∗=7.0. The 1 in 100 year daily average top plate current is a factor of 1.2 to 1.8 larger than the corresponding 1 in 10 year current. The 1 in 10 year daily average middle and bottom plate currents also decrease with increasing L∗ ranging from 0.4 pA cm−2 at L∗=4.75 to 0.01 pA cm−2 at L∗=7.0. The 1 in 100 year daily average middle and bottom plate currents are a factor of 1.2 to 2.7 larger than the corresponding 1 in 10 year currents. Averaged along the orbit path the 1 in 10 year daily average top, middle, and bottom plate currents are 0.22, 0.094, and 0.094 pA cm−2, respectively

Results from Testing Low-Cost, High-Performance Terrestrial Processors for Use in Low-Cost High-Performance Space Missions

There has been a significant and exciting increase in the use of microsatellites and cubesats in the past decade. However, it has proved difficult to scale up current cubesat avionics systems to enable larger, longer, more complex missions, and challenging to scale down traditional microsatellites to an affordable price point. The need exists for a system that provides the capability of a microsatellite at a cubesat cost; KISPE Space (“KISPE”) is developing the Next Generation Microsatellite Platform (“NGMP”) to address this need and is releasing the design as an open source resource via the Open Source Satellite Programme (“OSSAT”) A key enabler of developing a robust Next Generation Microsatellite Platform is the identification of a suitable low-cost microprocessor that can be used to form the foundation of an affordable, robust, flexible, performant and autonomous satellite platform avionics system. Space-qualified, long-lifetime, radiation-tolerant (or hardened) processors do exist, however, these technologies are very expensive and tend to deliver poor mission performance compared to the latest terrestrial Commercial-Off-The-Shelf (COTS) components and are not compatible with the limited resources available from cubesats and smallsats. We performed a test campaign to identify one or more commercially available microprocessors that leverage the latest innovations in microprocessor technology and which meet a set of system criteria that make them suitable for use as a microsatellite platform processor for a wide range of missions; from single modest spacecraft, through to proliferated architectures requiring autonomous operations. We are sharing these test results freely with the space community to advance small satellite capabilities and to stimulate the development of the next wave of cost-effective missions, applications and services. Three COTS processors (SAMV71, STM32H7 and SAMA5D3) were downselected for Total Ionising Dose (electron) radiation testing to characterize their performance in a representative space radiation environment, in partnership with the University of Surrey and with the input of OSSA T collaborators. All three processors were deemed to be candidates for further evaluation and derisking: The devices began to fail at 60kRads, 47kRads and in excess of 120kRads respectively

Innovative Teaching Strategies and Conventional Approaches for Enhanced Learning in a Global Information Environment

This SIG session features five panels that will share innovative ideas on teaching and learning in LIS. Each panel will showcase a novel approaches to pedagogy that attendees will find useful. Agosto and Poole discuss Community-Based Librarianship, a postbaccalaureate certificate program being developed at Drexel University. In Determining Community Needs with CARES, Bossaller, Adkins, and Kleinsorge demonstrate how the CARES Engagement Network, a free online resource, can be used in the LIS curriculum. Hands and Tucker discuss The 7-Slide Update: A Pedagogical Tool for Enriching Scholarly Communication, a guided approach that focuses on key dimensions of doctoral work. Alman and Faires provide an overview of the social media apps in use by iSchool faculty at San Jose State University in Extend Learning Beyond the Classroom with Social Media & Cloud-based Apps: Connecting, Communicating and Transforming LIS Education. In Social Justice Design and Implementation: Transforming LIS Education. Mehra discusses his critical pedagogies and reflective practices as an instructor of three graduate courses taught in LIS at the University of Alabama. Presentations will be followed by an interactive question and answer session

Radiation effects on satellites during extreme space weather events

High‐energy trapped electrons in the Van Allen belts pose a threat to the survivability of orbiting spacecraft. Two key radiation effects are total ionising dose (TID) and displacement damage dose (DDD) in components and materials, both of which cause cumulative and largely irreversible damage. During an extreme space weather event, trapped electron fluxes in the Van Allen belts can increase by several orders of magnitude in intensity, leading to an enhanced risk of satellite failure. We use extreme environments generated by modelling and statistical analyses to estimate the consequences for satellites in terms of the radiation effects described above. A worst‐case event could lead to significant losses in power generating capability ‐ up to almost 8% ‐ and cause up to four years’ worth of ionising dose degradation, leading to component damage and a life‐shortening effect on satellites. The consequences of such losses are hugely significant given our increasing reliance on satellites for a vast array of services, including communication, navigation, defence and critical infrastructure

Realistic worst case for a severe space weather event driven by a fast solar wind stream

Satellite charging is one of the most important risks for satellites on orbit. Satellite charging can lead to an electrostatic discharge resulting in component damage, phantom commands, and loss of service and in exceptional cases total satellite loss. Here we construct a realistic worst case for a fast solar wind stream event lasting 5 days or more and use a physical model to calculate the maximum electron flux greater than 2 MeV for geostationary orbit. We find that the flux tends toward a value of 106 cm−2·s−1·sr−1 after 5 days and remains high for another 5 days. The resulting flux is comparable to a 1 in 150‐year event found from an independent statistical analysis of electron data. Approximately 2.5 mm of Al shielding would be required to reduce the internal charging current to below the National Aeronautics and Space Administration‐recommended guidelines, much more than is currently used. Thus, we would expect many satellites to report electrostatic discharge anomalies during such an event with a strong likelihood of service outage and total satellite loss. We conclude that satellites at geostationary orbit are more likely to be at risk from fast solar wind stream event than a Carrington‐type storm

Analysis of the interaction with the hepatitis C virus mRNA reveals an alternative mode of RNA recognition by the human La protein

Human La protein is an essential factor in the biology of both coding and non-coding RNAs. In the nucleus, La binds primarily to 3′ oligoU containing RNAs, while in the cytoplasm La interacts with an array of different mRNAs lacking a 3′ UUUOH trailer. An example of the latter is the binding of La to the IRES domain IV of the hepatitis C virus (HCV) RNA, which is associated with viral translation stimulation. By systematic biophysical investigations, we have found that La binds to domain IV using an RNA recognition that is quite distinct from its mode of binding to RNAs with a 3′ UUUOH trailer: although the La motif and first RNA recognition motif (RRM1) are sufficient for high-affinity binding to 3′ oligoU, recognition of HCV domain IV requires the La motif and RRM1 to work in concert with the atypical RRM2 which has not previously been shown to have a significant role in RNA binding. This new mode of binding does not appear sequence specific, but recognizes structural features of the RNA, in particular a double-stranded stem flanked by single-stranded extensions. These findings pave the way for a better understanding of the role of La in viral translation initiation

Heterodimerization of the human RNase P/MRP subunits Rpp20 and Rpp25 is a prerequisite for interaction with the P3 arm of RNase MRP RNA

Rpp20 and Rpp25 are two key subunits of the human endoribonucleases RNase P and MRP. Formation of an Rpp20–Rpp25 complex is critical for enzyme function and sub-cellular localization. We present the first detailed in vitro analysis of their conformational properties, and a biochemical and biophysical characterization of their mutual interaction and RNA recognition. This study specifically examines the role of the Rpp20/Rpp25 association in the formation of the ribonucleoprotein complex. The interaction of the individual subunits with the P3 arm of the RNase MRP RNA is revealed to be negligible whereas the 1:1 Rpp20:Rpp25 complex binds to the same target with an affinity of the order of nM. These results unambiguously demonstrate that Rpp20 and Rpp25 interact with the P3 RNA as a heterodimer, which is formed prior to RNA binding. This creates a platform for the design of future experiments aimed at a better understanding of the function and organization of RNase P and MRP. Finally, analyses of interactions with deletion mutant proteins constructed with successively shorter N- and C-terminal sequences indicate that the Alba-type core domain of both Rpp20 and Rpp25 contains most of the determinants for mutual association and P3 RNA recognition

Basic science232. Certolizumab pegol prevents pro-inflammatory alterations in endothelial cell function

Background: Cardiovascular disease is a major comorbidity of rheumatoid arthritis (RA) and a leading cause of death. Chronic systemic inflammation involving tumour necrosis factor alpha (TNF) could contribute to endothelial activation and atherogenesis. A number of anti-TNF therapies are in current use for the treatment of RA, including certolizumab pegol (CZP), (Cimzia ®; UCB, Belgium). Anti-TNF therapy has been associated with reduced clinical cardiovascular disease risk and ameliorated vascular function in RA patients. However, the specific effects of TNF inhibitors on endothelial cell function are largely unknown. Our aim was to investigate the mechanisms underpinning CZP effects on TNF-activated human endothelial cells. Methods: Human aortic endothelial cells (HAoECs) were cultured in vitro and exposed to a) TNF alone, b) TNF plus CZP, or c) neither agent. Microarray analysis was used to examine the transcriptional profile of cells treated for 6 hrs and quantitative polymerase chain reaction (qPCR) analysed gene expression at 1, 3, 6 and 24 hrs. NF-κB localization and IκB degradation were investigated using immunocytochemistry, high content analysis and western blotting. Flow cytometry was conducted to detect microparticle release from HAoECs. Results: Transcriptional profiling revealed that while TNF alone had strong effects on endothelial gene expression, TNF and CZP in combination produced a global gene expression pattern similar to untreated control. The two most highly up-regulated genes in response to TNF treatment were adhesion molecules E-selectin and VCAM-1 (q 0.2 compared to control; p > 0.05 compared to TNF alone). The NF-κB pathway was confirmed as a downstream target of TNF-induced HAoEC activation, via nuclear translocation of NF-κB and degradation of IκB, effects which were abolished by treatment with CZP. In addition, flow cytometry detected an increased production of endothelial microparticles in TNF-activated HAoECs, which was prevented by treatment with CZP. Conclusions: We have found at a cellular level that a clinically available TNF inhibitor, CZP reduces the expression of adhesion molecule expression, and prevents TNF-induced activation of the NF-κB pathway. Furthermore, CZP prevents the production of microparticles by activated endothelial cells. This could be central to the prevention of inflammatory environments underlying these conditions and measurement of microparticles has potential as a novel prognostic marker for future cardiovascular events in this patient group. Disclosure statement: Y.A. received a research grant from UCB. I.B. received a research grant from UCB. S.H. received a research grant from UCB. All other authors have declared no conflicts of interes

Case Reports1. A Late Presentation of Loeys-Dietz Syndrome: Beware of TGFβ Receptor Mutations in Benign Joint Hypermobility

Background: Thoracic aortic aneurysms (TAA) and dissections are not uncommon causes of sudden death in young adults. Loeys-Dietz syndrome (LDS) is a rare, recently described, autosomal dominant, connective tissue disease characterized by aggressive arterial aneurysms, resulting from mutations in the transforming growth factor beta (TGFβ) receptor genes TGFBR1 and TGFBR2. Mean age at death is 26.1 years, most often due to aortic dissection. We report an unusually late presentation of LDS, diagnosed following elective surgery in a female with a long history of joint hypermobility. Methods: A 51-year-old Caucasian lady complained of chest pain and headache following a dural leak from spinal anaesthesia for an elective ankle arthroscopy. CT scan and echocardiography demonstrated a dilated aortic root and significant aortic regurgitation. MRA demonstrated aortic tortuosity, an infrarenal aortic aneurysm and aneurysms in the left renal and right internal mammary arteries. She underwent aortic root repair and aortic valve replacement. She had a background of long-standing joint pains secondary to hypermobility, easy bruising, unusual fracture susceptibility and mild bronchiectasis. She had one healthy child age 32, after which she suffered a uterine prolapse. Examination revealed mild Marfanoid features. Uvula, skin and ophthalmological examination was normal. Results: Fibrillin-1 testing for Marfan syndrome (MFS) was negative. Detection of a c.1270G > C (p.Gly424Arg) TGFBR2 mutation confirmed the diagnosis of LDS. Losartan was started for vascular protection. Conclusions: LDS is a severe inherited vasculopathy that usually presents in childhood. It is characterized by aortic root dilatation and ascending aneurysms. There is a higher risk of aortic dissection compared with MFS. Clinical features overlap with MFS and Ehlers Danlos syndrome Type IV, but differentiating dysmorphogenic features include ocular hypertelorism, bifid uvula and cleft palate. Echocardiography and MRA or CT scanning from head to pelvis is recommended to establish the extent of vascular involvement. Management involves early surgical intervention, including early valve-sparing aortic root replacement, genetic counselling and close monitoring in pregnancy. Despite being caused by loss of function mutations in either TGFβ receptor, paradoxical activation of TGFβ signalling is seen, suggesting that TGFβ antagonism may confer disease modifying effects similar to those observed in MFS. TGFβ antagonism can be achieved with angiotensin antagonists, such as Losartan, which is able to delay aortic aneurysm development in preclinical models and in patients with MFS. Our case emphasizes the importance of timely recognition of vasculopathy syndromes in patients with hypermobility and the need for early surgical intervention. It also highlights their heterogeneity and the potential for late presentation. Disclosures: The authors have declared no conflicts of interes

Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease

Background: Abnormal handling of E. coli by lamina propria (LP) macrophages may contribute to Crohn’s disease (CD) pathogenesis. We aimed to determine LP macrophage phenotypes in CD, ulcerative colitis (UC) and healthy controls (HC), and in CD, to compare macrophage phenotypes according to E. coli carriage. Methods: Mucosal biopsies were taken from 35 patients with CD, 9 with UC and 18 HCs. Laser capture microdissection was used to isolate E. coli-laden and unladen LP macrophages from ileal or colonic biopsies. From these macrophages, mRNA was extracted and cytokine and activation marker expression measured using RT-qPCR. Results: E. coli-laden LP macrophages were identified commonly in mucosal biopsies from CD patients (25/35, 71 %), rarely in UC (1/9, 11 %) and not at all in healthy controls (0/18). LP macrophage cytokine mRNA expression was greater in CD and UC than healthy controls. In CD, E. coli-laden macrophages expressed high IL-10 & CD163 and lower TNFα, IL-23 & iNOS irrespective of macroscopic inflammation. In inflamed tissue, E. coli-unladen macrophages expressed high TNFα, IL-23 & iNOS and lower IL-10 & CD163. In uninflamed tissue, unladen macrophages had low cytokine mRNA expression, closer to that of healthy controls. Conclusion: In CD, intra-macrophage E. coli are commonly found and LP macrophages express characteristic cytokine mRNA profiles according to E. coli carriage. Persistence of E. coli within LP macrophages may provide a stimulus for chronic inflammation