A DESCONSIDERAÇÃO INVERSA DA PERSONALIDADE JURÍDICA: UMA ANÁLISE DA APLICABILIDADE DA LEI DA LIBERDADE ECONÔMICA NO CASO DO DEVEDOR DE ALIMENTOS

O presente trabalho analisa a Lei 13.874/2019, chamada de Declaração de Direitos da Liberdade Econômica, sob o viés da desconsideração da personalidade jurídica inversa, especificamente identificando sua aplicabilidade no caso de obrigações alimentares, em que o devedor pratica atos de ocultação patrimonial. A Lei da Liberdade Econômica, inicialmente concebida pela Medida Provisória de nº 881, proclama ideais neoliberais de mercado, temas tidos como centrais pelo atual governo. Por sua vez, a desconsideração da personalidade jurídica inversa, prevista na mencionada lei, constava unicamente de construção jurisprudencial e agora é regulamentada pelo artigo 50, §3º, do Código Civil. Por meio do presente artigo, verificou-se ser possível a desconsideração da personalidade jurídica inversa, na hipótese do devedor de alimentos que utiliza da pesssoa jurídica para tornar-se inadimplente com seus deveres alimentares. Assim, tal medida é necessária para efetivar o direito do alimentando. Para isso, qualifica-se a pesquisa como quantitativa e qualitativa e de método indutivo. A pesquisa caracteriza-se quanto seus objetivos, como descritiva.&nbsp

Prognostic impact of pretransplant measurable residual disease assessed by peripheral blood WT1-mRNA expression in patients with AML and MDS

OBJECTIVE As peripheral blood (PB) Wilm's Tumor 1 (WT1)-mRNA expression is established as MRD-marker during conventional AML chemotherapy, impact of pretransplant WT1 expression remains unclear. Therefore, we aimed to assess prognostic impact of pretransplant WT1 expression on post-transplant outcome in patients with AML/MDS. METHODS In 64 AML/MDS patients, pretransplant WT1 expression was retrospectively analyzed using a standardized assay offering high sensitivity, specificity, and a validated cut-off. Patients were divided into three groups determined by pretransplant remission and WT1 expression. Post-transplant outcome of these groups was compared regarding cumulative incidence of relapse (CIR), relapse-free (RFS), and overall survival (OS). RESULTS Pretransplant forty-six patients (72%) showed hematologic remission, including 21 (46%) MRD-negative and 25 (54%) MRD-positive patients indicated by WT1 expression, while 18 refractory patients (28%) showed active disease. Two-year estimates of post-transplant CIR, RFS, and OS were similar in MRD-positive (61%, 37%, 54%) and refractory patients (70%, 26%, 56%), but significantly inferior compared with MRD-negative patients (10%, 89%, 90%). After multivariable adjustment, pretransplant MRD negativity measured by WT1 expression retained its prognostic impact on CIR (P~=~.008), RFS (P~=~.005), and OS (P~=~.049). CONCLUSIONS PB WT1 expression represents a useful method to estimate pretransplant MRD, which is highly predictable for post-transplant outcome and may help improving peri-transplant management in AML/MDS patients

DELIMITAÇÃO E CARACTERIZAÇÃO DE APP ATRAVÉS DO USO DE UM SISTEMA DE INFORMAÇÃO GEOGRÁFICA (SIG): O CASO DAS APP’S NOS CURSOS DE ÁGUA DA SUB-BACIA DO LAJEADO PARDO, NOROESTE DO RS

O uso de tecnologias como o Sistema de Informação Geográfica (SIG) tem eficácia na identificação e no controle de áreas específicas e podem ser implementadas em curto prazo em diferentes escalas. A utilização destas tecnologias vem crescendo rapidamente em todo o mundo, uma vez que ela possibilita um melhor gerenciamento de informações tornando possível analisar as mudanças ou problemas que ocorrem em um determinado local, permitindo a tomada de decisão em áreas de grande complexidade. O presente estudo utilizou o software Quantum GIS (QGIS) o qual é uma ferramenta do sistema de informação geográfica, para delimitar e caracterizar as Áreas de Preservação Permanente (APP) de uma sub-bacia do Lajeado Pardo, localizada entre os municípios de Frederico Westphalen e Taquaruçu do Sul no estado do Rio Grande do Sul

Survey of Campylobacter in foods implicated in foodborne diseases in Southern Brazil

Campylobacter is not routinely tested in foodborne disease investigations in Brazil. Here, we studied the occurrence of Campylobacter among other food-related bacteria commonly found in foodborne disease outbreaks reported in Rio Grande do Sul State, Southern Brazil. Seventy-two food samples were analyzed by using culture-based detection methods during the 18-month investigation of 36 foodborne disease outbreaks. The sampled foods from the foodborne disease outbreaks were all negative for Campylobacter. However, at least one of other routinely investigated foodborne-related bacteria was detected in 29.17% (21/72) of the samples. Taken together, these results suggest the need to monitor Campylobacter in foodborne diseases to detect sporadic cases caused by Campylobacter that might go unnoticed in Rio Grande do Sul

Electrocatalytic Reduction of CO2 to Acetic Acid by a Molecular Manganese Corrole Complex

The controlled electrochemical reduction of carbon dioxide to value added chemicals is an important strategy in terms of renewable energy technologies. Therefore, the development of efficient and stable catalysts in an aqueous environment is of great importance. In this context, we focused on synthesizing and studying a molecular MnIII-corrole complex, which is modified on the three meso-positions with polyethylene glycol moieties for direct and selective production of acetic acid from CO2. Electrochemical reduction of MnIII leads to an electroactive MnII species, which binds CO2 and stabilizes the reduced intermediates. This catalyst allows to electrochemically reduce CO2 to acetic acid in a moderate acidic aqueous medium (pH 6) with a selectivity of 63 % and a turn over frequency (TOF) of 8.25 h−1, when immobilized on a carbon paper (CP) electrode. In terms of high selectivity towards acetate, we propose the formation and reduction of an oxalate type intermediate, stabilized at the MnIII-corrole center. © 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhei

New tendencies in non-surgical periodontal therapy

The aim of this review was to update the evidence of new approaches to non-surgical therapy (NSPT) in the treatment of periodontitis. Preclinical and clinical studies addressing the benefits of adjunctive antimicrobial photodynamic therapy, probiotics, prebiotics/synbiotics, statins, pro-resolving mediators, omega-6 and -3, ozone, and epigenetic therapy were scrutinized and discussed. Currently, the outcomes of these nine new approaches, when compared with subgingival debridement alone, did not demonstrate a significant added clinical benefit. However, some of these new alternative interventions may have the potential to improve the outcomes of NSPT alone. Future evidence based on randomized controlled clinical trials would help clinicians and patients in the selection of different adjunctive therapies

Disruption of the vacuolar-type H+-ATPase complex in liver causes MTORC1-independent accumulation of autophagic vacuoles and lysosomes

The vacuolar-type H+-translocating ATPase (v-H+-ATPase) has been implicated in the amino aciddependent activation of the mechanistic target of rapamycin complex 1 (MTORC1), an important regulator of macroautophagy. To reveal the mechanistic links between the v-H+-ATPase and MTORC1, we destablilized v-H+-ATPase complexes in mouse liver cells by induced deletion of the essential chaperone ATP6AP2. ATP6AP2-mutants are characterized by massive accumulation of endocytic and autophagic vacuoles in hepatocytes. This cellular phenotype was not caused by a block in endocytic maturation or an impaired acidification. However, the degradation of LC3-II in the knockout hepatocytes appeared to be reduced. When v-H+-ATPase levels were decreased, we observed lysosome association of MTOR and normal signaling of MTORC1 despite an increase in autophagic marker proteins. To better understand why MTORC1 can be active when v-H+-ATPase is depleted, the activation of MTORC1 was analyzed in ATP6AP2-deficient fibroblasts. In these cells, very little amino acid-elicited activation of MTORC1 was observed. In contrast, insulin did induce MTORC1 activation, which still required intracellular amino acid stores. These results suggest that in vivo the regulation of macroautophagy depends not only on v-H+-ATPase-mediated regulation of MTORC1.Peer reviewe

Permissive and Restricted Virus Infection of Murine Embryonic Stem Cells

Recent RNA interference (RNAi) studies have identified many host proteins that modulate virus infection, but small interfering RNA 'off-target' effects and the use of transformed cell lines limit their conclusiveness. As murine embryonic stem (mES) cells can be genetically modified and resources exist where many and eventually all known mouse genes are insertionally inactivated, it was reasoned that mES cells would provide a useful alternative to RNAi screens. Beyond allowing investigation of host-pathogen interactions in vitro, mES cells have the potential to differentiate into other primary cell types, as well as being used to generate knockout mice for in vivo studies. However, mES cells are poorly characterized for virus infection. To investigate whether ES cells can be used to explore host-virus interactions, this study characterized the responses of mES cells following infection by herpes simplex virus type 1 (HSV-1) and influenza A virus. HSV-1 replicated lytically in mES cells, although mES cells were less permissive than most other cell types tested. Influenza virus was able to enter mES cells and express some viral proteins, but the replication cycle was incomplete and no infectious virus was produced. Knockdown of the host protein AHCYL1 in mES cells reduced HSV-1 replication, showing the potential for using mES cells to study host-virus interactions. Transcriptional profiling, however, indicated the lack of an efficient innate immune response in these cells. mES cells may thus be useful to identify host proteins that play a role in virus replication, but they are not suitable to determine factors that are involved in innate host defence

Efeitos do método pilates nos parâmetros de força e função pulmonar: uma revisão sistemática / Effects of the pilates method in forces and lung function parameters: a systematic review

O Método Pilates se popularizou nas últimas décadas, sendo necessária a investigação de seus benefícios para prática clínica. Este estudo teve por objetivo verificar os efeitos de programas de treinamento com método Pilates na força muscular respiratória e função pulmonar. Para tal, realizou-se uma revisão sistemática, nas bases de dados PubMed, LILACS, Cochrane-Library, PEDro e Science Direct, de acordo com as palavras do dicionário Medical Subject Heading Terms (MeSH) para PubMed e Cochrane-Library, adequadas para as demais bases. Foram incluídos: estudos de intervenção com Método Pilates; indivíduos de qualquer faixa etária; ambos os sexos; saudáveis ou não; avaliados por meio do exame de espirometria, manovacuometria ou outros testes de função pulmonar considerados relevantes; follow-up mínimo de 4 semanas. Ao final 10 estudos foram considerados elegíveis. Houve melhora significativa nos parâmetros de Força Muscular Inspiratória (PImáx) em 7 estudos, que contemplaram indivíduos saudáveis, com Doença Renal Crônica (DRC) e Fibrose Cística (FC). Em relação a Força Muscular Expiratória (PEmáx), o Método Pilates realizado isoladamente, demonstrou melhora significativa nos parâmetros de PEmáx em 7 estudos, em indivíduos saudáveis, de ambos os sexos e diferentes faixas etárias. As variáveis de função pulmonar Pico de fluxo Expiratório (PFE) e ventilação voluntária máxima (VVM), evidenciaram aumento significativo em 1 estudo e em 2 estudos, respectivamente, ambos em mulheres saudáveis jovens. Podemos concluir que o Método Pilates demonstrou influenciar positivamente os parâmetros de PImáx, PEmáx, PFE e VVM em indivíduos saudáveis de ambos os sexos e diferentes faixas etárias. Adicionalmente, foi capaz de promover melhora na PImáx de pacientes com DRC e FC.