294 research outputs found
Molecular Marker Linkage Map for Apple
Linkage maps for two apple clones, White Angel and Rome Beauty, were constructed using isozyme and DNA polymorphisms segregating in a population produced from a Rome Beauty Ă White Angel cross. The linkage map for White Angel consists of 253 markers arranged in 24 linkage groups and extends over 950 cM. The Rome Beauty map contains 156 markers on 21 linkage groups. The White Angel map was taken as the standard, and we were able to identify linkage groups in Rome Beauty homologous to 13 White Angel linkage groups. The location of several genes not segregating in the Rome Beauty Ă White Angel population could be determined on the basis of known linkages with segregating markers. Hence, the standard map for apple now contains about 360 markers, with most linkage groups saturated at 10-15 cM. The double pseudotestcross format of the mapping population permitted the comparison of recombination frequencies in male and female parents in certain regions of the genome where appropriate markers were available. The recombination frequencies observed for the approximately 170 cM that were comparable gave no indication that a sex-related difference in recombination rate was characteristic of appl
Pantic B-spline wavelets and their application for solving linear integral equations
Abstract In this work we deal with the question: how can one improve the approximation level for some nonlinear integral equations? Good candidates for this aim are semi orthogonal B-spline scaling functions and their duals. Although there are different works in this area, only B-spline of degree at most 2 are used for this approximation. Here we compute B-spline scaling functions of degree 4 and their duals, then we will show that, by using them, one can have better approximation results for the solution of integral equations in comparison with less degrees or other kinds of scaling functions. Some numerical examples show their attractiveness and usefulness
Accumulation of lead and zinc by plants colonizing a metal mining area in Central Iran
The Irankouh area, located in Central Iran, is a vast mountainous region with mineralized soils and several active zinc and lead mining and smelting sites. In this study plants and soils from 5 different sites in this area were collected and analyzed for Zn and Pb. Analysis of soils from different sites showed the expected high concentrations of Zn and Pb - up to 23,000 and 18,000 ÎŒg g-1 for total, 30 and 20 ÎŒg g-1 for exchangeable, 1 and 0.6 ÎŒg g-1 for water-soluble fractions, respectively. Plants collected from these sites total 67 species from 66 genera and 29 families. Most of these are annual herbs found also on non-metalliferous soils in this region. The concentrations of Zn and Pb in the leaf dry matter of plants were variable, with up to 4800 ÎŒg g-1 for Zn and 740 ÎŒg g-1 Pb in Matthiola chenopodiifolia and Pinus elderica, respectively. A significant positive correlation was detected between the concentrations of Zn and Pb in plant dry matter and those in soils. The concentrations of Zn and Pb in the leaves of most species collected were significantly higher than for other plants from non-metalliferous soils. Some accumulator plants found in this area could have potential for soil clean-up by phytoextraction
Neutrophils, Crucial, or Harmful Immune Cells Involved in Coronavirus Infection: A Bioinformatics Study
The latest member of the Coronaviridae family, called SARS-CoV-2, causes the Coronavirus Disease 2019 (COVID-19). The disease has caused a pandemic and is threatening global health. Similar to SARS-CoV, this new virus can potentially infect lower respiratory tract cells and can go on to cause severe acute respiratory tract syndrome, followed by pneumonia and even death in many nations. The molecular mechanism of the disease has not yet been evaluated until now. We analyzed the GSE1739 microarray dataset including 10 SARS-positive PBMC and four normal PBMC. Co-expression network analysis by WGCNA suggested that highly preserved 833 turquoise module with genes were significantly related to SARS-CoV infection. ELANE, ORM2, RETN, BPI, ARG1, DEFA4, CXCL1, and CAMP were the most important genes involved in this disease according to GEO2R analysis as well. The GO analysis demonstrated that neutrophil activation and neutrophil degranulation are the most activated biological processes in the SARS infection as well as the neutrophilia, basophilia, and lymphopenia predicted by deconvolution analysis of samples. Thus, using Serpins and Arginase inhibitors during SARS-CoV infection may be beneficial for increasing the survival of SARS-positive patients. Regarding the high similarity of SARS-CoV-2 to SARS-CoV, the use of such inhibitors might be beneficial for COVID-19 patients
Anaerobic co-digestion of oil refinery wastewater and chicken manure to produce biogas, and kinetic parameters determination in batch reactors
ArticleIn order to improve the anaerobic fermentation of oil refinery wastewater (ORWW)
via an appropriate nutrients pool for microbial and buffer capacity growth, a study was carried
out on related anaerobic co-digestion (AcoD) with a rich organic carbon source, namely chicken
manure (CM). The kinetic parameters were investigated (including cumulative biogas production,
bio-methane content, retention time, and soluble chemical oxygen demand stabilisation rate) of
batch AcoD experiments related to six ORWW:CM-ratio treatments (5:0, 4:1, 3:2, 2:3, 1:4, and
0:5) under mesophilic conditions. The highest soluble chemical oxygen demand removal rate was
obtained for the 4:1-ratio treatment. However, the highest biogas production and bio-methane
contents were achieved for the 1:4-ratio treatment. When taking into consideration the highest oil
refinery wastewater portion in the AcoD mixtures and the statistical test results (LSD0.05) for the
kinetic parameters, it can be seen that the 4:1-ratio treatment provided the maximum biogas
production levels
Time-domain numerical modeling of THz receivers based on photoconductive antennas
We present here a simulator that solves the main semiconductor charge and transport equations coupled to Maxwell equations to study receivers based on photoconductive antennas (R-PCAs). Making use of this tool we were able to correctly characterize the operation of these antennas. In doing so, we compared simulations with the results of the semi-empirical expression ITHz(t) â Ïc(t) â ETHz(t) employed to evaluate the detected photo-current by means of the convolution between the photoconductivity in the receiver and the electric field linked to the emitter antenna. We were able to accurately reproduce experimental data with our simulation tool. These kind of tools are essential to model photoconductive antennas, a fundamental step needed for the development of terahertz time domain spectroscopy (THz-TDS) applications based on PCAs.The work described in this paper is partially funded by the Spanish
National Projects TEC2013-48414-C3-01, CSD2008-00068, P09-TIC-5327, P12-TIC-1442, and by the GENIL excellence network
Methylene tetrahydrofolate reductase, transforming growth factor-ÎČ1 and lymphotoxin-α genes polymorphisms and susceptibility to rheumatoid arthritis
AbstractBackgroundRheumatoid arthritis is a widely prevalent autoimmune disorder with suggested genetic predisposition.ObjectivesThe aim of this study is to detect the pattern of genetic polymorphism of methylene tetrahydrofolate reductase (MTHFR C677 T and A1298 C), transforming growth factor-ÎČ1 (TGF-ÎČ1 T869 C) and lymphotoxin-α (LT-α A252G) in patients having rheumatoid arthritis and correlate these patterns to disease activity and serum levels of tumor necrosis factor-alpha (TNF-α), B-Cell Activating Factor (BAFF), and osteopontin.MethodsA total of 194 subjects, 90 controls and 104 patients with rheumatoid arthritis were genotyped for MTHFR C677 T and A1298 C, TGF-ÎČ1 T869 C and LT-α A252G polymorphisms using a methodology based on PCR-RFLP. Also serum levels of TNF-α, osteopontin and BAFF were measured by ELISA kits.ResultsThe CT genotype and T allele of MTHFR C677 T and GG genotype and G allele of LT-α A252G are associated with the risk of RA and with higher levels of the pro-inflammatory cytokine, TNF-α in patients with rheumatoid arthritis.ConclusionOur findings suggest that there is association between MTHFR C677 T and LT-α A252G genes polymorphisms and increased risk of RA in this sample of Egyptian population
Polimorfismos dos genes metilenotetrahidrofolato redutase, fator de crescimento transformador ÎČ1 e linfotoxinaâα e susceptibilidade Ă artrite reumatoide
ResumoAntecedentesA artrite reumatoide Ă© uma doença autoimune amplamente prevalente com sugerida predisposição genĂ©tica.ObjetivosDetectar o padrĂŁo de polimorfismo dos genes metilenotetrahidrofolato redutase (MTHFR C677T e A1298C), fator de crescimento transformador ÎČ1 (TGFâÎČ1 T869C) e linfotoxinaâα (LTâα A252G) em pacientes com artrite reumatoide e correlacionar esses padrĂ”es com a atividade da doença e os nĂveis sĂ©ricos de fator de necrose tumoral alfa (TNFâα), fator ativador de linfĂłcitos B (BAFF) e osteopontina.MĂ©todosForam genotipados 194 indivĂduos â 90 controles e 104 com artrite reumatoide â Ă procura de polimorfismos dos genes MTHFR C677T e A1298C, TGFâÎČ1 T869C e LTâα A252G com uma metodologia baseada na PCRâRFLP. Mensuraramâse tambĂ©m os nĂveis sĂ©ricos de TNFâα, osteopontina e BAFF com kits de Elisa.ResultadosO genĂłtipo CT e o alelo T do MTHFR C677T e o genĂłtipo GG e alelo G do LTâα A252G estĂŁo associados ao risco de AR e a nĂveis mais elevados da citocina prĂłâinflamatĂłria TNFâα em pacientes com artrite reumatoide.ConclusĂŁoOs achados do presente estudo sugerem que hĂĄ associação entre os polimorfismos dos genes MTHFR C677T e LTâα A252G e um risco aumentado de AR nessa amostra da população egĂpcia.AbstractBackgroundRheumatoid arthritis is a widely prevalent autoimmune disorder with suggested genetic predisposition.ObjectivesThe aim of this study is to detect the pattern of genetic polymorphism of methylene tetrahydrofolate reductase (MTHFR C677T and A1298C), transforming growth factorâÎČ1 (TGFâÎČ1 T869C) and lymphotoxinâα (LTâα A252G) in patients having rheumatoid arthritis and correlate these patterns to disease activity and serum levels of tumor necrosis factorâalpha (TNFâα), BâCell Activating Factor (BAFF), and osteopontin.MethodsA total of 194 subjects, 90 controls and 104 patients with rheumatoid arthritis were genotyped for MTHFR C677T and A1298C, TGFâÎČ1 T869C and LTâα A252G polymorphisms using a methodology based on PCRâRFLP. Also serum levels of TNFâα, osteopontin and BAFF were measured by ELISA kits.ResultsThe CT genotype and T allele of MTHFR C677T and GG genotype and G allele of LTâα A252G are associated with the risk of RA and with higher levels of the proâinflammatory cytokine, TNFâα in patients with rheumatoid arthritis.ConclusionOur findings suggest that there is association between MTHFR C677T and LTâα A252G genes polymorphisms and increased risk of RA in this sample of Egyptian population
Radar remote sensing for crop classification and canopy condition assessment: Ground-data documentation
A vegetation and soil-moisture experiment was conducted in order to examine the microwave emission and backscattering from vegetation canopies and soils. The data-acquisition methodology used in conjunction with the mobile radar scatterometer (MRS) systems is described and associated ground-truth data are documented. Test fields were located in the Kansas River floodplain north of Lawrence, Kansas. Ten fields each of wheat, corn, and soybeans were monitored over the greater part of their growing seasons. The tabulated data summarize measurements made by the sensor systems and represent target characteristics. Target parameters describing the vegetation and soil characteristics include plant moisture, density, height, and growth stage, as well as soil moisture and soil-bulk density. Complete listings of pertinent crop-canopy and soil measurements are given
Ligating Behavior Of Some Sulphur Containing Benzotriazole Derivatives Towards Some Transition Metal Ions And Their Biological Effect
Abstract: New Cu 2+ and Ni 2+ complexes of N 1 -phenyl-2-[1H-1,2,3-bezotriazol-1-yl] 3-phenyl-3-oxopropane thioamide, HL, has been synthesized and characterized by different spectral and magnetic measurements and elemental analysis. The spectral studies indicated that HL exist in the thion form in the solid state and the IR spectra of the complexes indicated that the ligand act as monobasic bidentate ligand giving distorted tetragonal structure in case of Cu 2+ and square planar structure in case of Ni 2+ , which was the reason of their different antimicrobial activity. Thermal decomposition of both complexes showed similar steps
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