6,698 research outputs found

    Modular and adaptable tumor niche prepared from visible light-initiated thiol-norbornene photopolymerization

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    Photopolymerized biomimetic hydrogels with adaptable properties have been widely used for cell and tissue engineering applications. As a widely adopted gel crosslinking method, photopolymerization provides experimenters on-demand and spatial-temporal controls in gelation kinetics. Long wavelength ultraviolet (UV) light initiated photopolymerization is among the most popular methods in the fabrication of cell-laden hydrogels owing to its rapid and relatively mild gelation conditions. The use of UV light, however, still causes concerns regarding its potential negative impacts on cells. Alternatively, visible light based photopolymerization can be used to crosslink cell-laden hydrogels. The majority of visible light based gelation schemes involve photoinitiator, co-initiator, and co-monomer. This multi-component initiation system creates added challenges for optimizing hydrogel formulations. Here, we report a co-initiator/co-monomer-free visible light initiated thiol-norbornene photopolymerization scheme to prepare modular biomimetic hydrogels suitable for in situ cell encapsulation. Eosin-Y was used as the sole initiator to initiate modular gelation between synthetic macromers (e.g., thiolated poly(vinyl alcohol) or poly(ethylene glycol)) and functionalized extracellular matrices (ECM), including norbornene-functionalized gelatin (GelNB) and/or thiolated hyaluronic acid (THA). These components are modularly crosslinked to afford bio-inert (i.e., purely synthetic), bioactive (i.e., using gelatin), and biomimetic (i.e., using gelatin and hyaluronic acid) hydrogels. The stiffness of the hydrogels can be easily tuned without affecting the contents of the bioactive components. Furthermore, the use of naturally-derived biomacromolecules (e.g., gelatin and HA) renders these hydrogels susceptible to enzyme-mediated degradation. In addition to demonstrating efficient and tunable visible light mediated gelation, we also utilized this biomimetic modular gelation system to formulate artificial tumor niche and to study the effects of cell density and gel modulus on the formation of pancreatic ductal adenocarcinoma (PDAC) spheroids.

    The Hidden Inconsistencies Introduced by Predictive Algorithms in Judicial Decision Making

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    Algorithms, from simple automation to machine learning, have been introduced into judicial contexts to ostensibly increase the consistency and efficiency of legal decision making. In this paper, we describe four types of inconsistencies introduced by risk prediction algorithms. These inconsistencies threaten to violate the principle of treating similar cases similarly and often arise from the need to operationalize legal concepts and human behavior into specific measures that enable the building and evaluation of predictive algorithms. These inconsistencies, however, are likely to be hidden from their end-users: judges, parole officers, lawyers, and other decision-makers. We describe the inconsistencies, their sources, and propose various possible indicators and solutions. We also consider the issue of inconsistencies due to the use of algorithms in light of current trends towards more autonomous algorithms and less human-understandable behavioral big data. We conclude by discussing judges and lawyers' duties of technological ("algorithmic") competence and call for greater alignment between the evaluation of predictive algorithms and corresponding judicial goals

    Compatibility of Gauss maps with metrics

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    We give necessary and sufficient conditions on a smooth local map of a Riemannian manifold MmM^m into the sphere SmS^m to be the Gauss map of an isometric immersion u:Mm→Rnu:M^m \to R^n, n=m+1n=m+1. We briefly discuss the case of general nn as wellComment: 14 pages, no figure

    Orientation of Vortices in a Superconducting Thin-Film: Quantitative Comparison of Spin-Polarized Neutron Reflectivity and Magnetization

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    We present a quantitative comparison of the magnetization measured by spin-polarized neutron reflectivity (SPNR) and DC magnetometry on a 1370 \AA\ -thick Nb superconducting film. As a function of magnetic field applied in the film plane, SPNR exhibits reversible behavior whereas the DC magnetization shows substantial hysteresis. The difference between these measurements is attributed to a rotation of vortex magnetic field out of the film plane as the applied field is reduced. Since SPNR measures only the magnetization parallel to the film plane whereas DC magnetization is strongly influenced by the perpendicular component of magnetization when there is a slight sample tilt, combining the two techniques allows one to distinguish two components of magnetization in a thin film.Comment: 12 pages, 8 figures, It will be printed in PRB, Oct. 200

    Winning the Peace Locally: UN Peacekeeping and Local Conflict

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    It remains contested whether peacekeeping works. The impact of peacekeepers? actions at the local (or subnational) level for overall mission success has lately received critical attention. Local peacekeeping is expected to matter because it re-assures local actors, deters resumption of armed hostilities, coerces parties to halt fighting, and makes commitment to agreements credible. Thus peacekeepers affect the relations between central and local elites and avoid the emergence of local power vacuums and areas of lawlessness. This study uses new subnational data on the deployment of United Nations peacekeepers. It uses matching and recursive bivariate probit models with exogenous variables for temporal and spatial variation to deal with possible non-random assignment of the treatment. It is demonstrated that conflict episodes last shorter when peacekeepers are deployed to conflict-prone locations inside a country, even with comparatively modest deployment. The effect of peacekeeping on the onset of local conflict is, however, less clear-cut

    Spin-polarized neutron reflectivity: A probe of vortices in thin-film superconductors

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    URL:http://link.aps.org/doi/10.1103/PhysRevB.59.14692 DOI:10.1103/PhysRevB.59.14692It is demonstrated that the specular reflectivity of spin-polarized neutrons can be used to study vortices in a thin-film superconductor. Experiments were performed on a 6000 Å thick c-axis film of YBa2Cu3O7-x with the magnetic field applied parallel to the surface. A magnetic hysteresis loop was observed for the spin-polarized reflection and, from these data, the average density of vortices was extracted. A model is presented which relates the specular reflectivity to the one-dimensional spatial distribution of vortices in the direction perpendicular to the surface. Unlike other techniques, neutron reflectivity observes vortices in a geometry where they are parallel to the interface.Support ~P.F.M., S.W.H.! from the Midwest Superconductivity Consortium ~MISCON! under the U.S. DOE Grant No. DE-FG02-90ER45427, the NSF DMR Grant No. 96-23827, and ~L.H.G., E.P.! from the NSF DMR Grant No. 94-21957, and ONR Grant No. N-00014-95-1-0831 is gratefully acknowledged. We thank E. Fullerton for useful discussions and D.H. Lowndes for help in understanding the surface roughness of oxide superconductors

    Health Links: Who Acts as a Source of Health Information in a Social Network?

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    Background: Members of a social network can influence the preventive health choices and cancer screening behaviors of other network members. Study Design: We conducted in-person interviews with 438 insured adults ages 40-70 in Massachusetts, Hawaii, and Georgia. We gathered information on social network communication regarding routine health topics and cancer screening. Participants reported whether family members and friends ask them for information or advice on health topics. Characteristics of each respondent’s social network were explored, including number of people with whom the participant has discussed colorectal cancer (CRC) screening. Participants were asked whether communication with social network members had ever led them to seek cancer screening. Principal Findings:80% of respondents in our group described themselves as a source of health information or advice for others in their social network (89% of women vs. 68% of men, p Conclusions: People who identified themselves as a source of health information within their social network were more likely to have discussed CRC screening with others and communicated with more people. Further study is necessary to understand the roles played by these individuals. As “health information ambassadors,” they may be effective targets for interventions that promote preventive screening

    The Supersymmetric Effective Action of the Heterotic String in Ten Dimensions

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    We construct the supersymmetric completion of quartic R+R4R+R^4-actions in the ten-dimensional effective action of the heterotic string. Two invariants, of which the bosonic parts are known from one-loop string amplitude calculations, are obtained. One of these invariants can be generalized to an R+F2+F4R+F^2+F^4-invariant for supersymmetric Yang-Mills theory coupled to supergravity. Supersymmetry requires the presence of B∧R∧R∧R∧RB\wedge R\wedge R\wedge R\wedge R-terms, (B∧F∧F∧F∧FB\wedge F\wedge F\wedge F\wedge F for Yang-Mills) which correspond to counterterms in the Green-Schwarz anomaly cancellation. Within the context of our calculation the ζ(3)R4\zeta(3)R^4-term from the tree-level string effective action does not allow supersymmetrization.Comment: 42 pages, UG-9/9
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