100 research outputs found
Microbiological Quality of Raw Goat Milk in Bogor, Indonesia
Milk samples were investigated for counts and prevalence of indicator bacteria, which were TPC, coliforms, coagulase positive Staphylococci (CPS), and coagulase negative Staphylococci (CNS). Ten potential risk factors were also evaluated in relation to the prevalence of indicator bacteria. The results showed that the median values of indicator bacterial counts from overall udder-half milk samples were 3.74, 0.70, 1.70, and 2.52 log cfu/ml and from bulk milk samples were 5.69, 2.98, 3.66 and 3.32 log cfu/ml for TPC, coliforms, CPS, and CNS, respectively. None of the median values of overall udder-half milk samples exceeded the maximum limit of the standards for all indicator bacteria. However, in the bulk milk samples only the median value of TPC below the maximum limit of the standards. Overall prevalence of coliforms, CPS and CNS from udder-half milk samples were 46.3%, 37.7%, and 66.0%, respectively, and from bulk milk samples were 86.7%, 76.7%, and 86.7%, respectively. Saanen crossbreed, fifth parity and udders with inflammation were found to be risk factors. This study results indicated that the hygienic practices in the dairy goat farms are still need to be increased. To increase the hygienic level of the milk, the identified significant risk factors must be controlled
insights by genetic characterization
Background Giardia duodenalis is a common flagellated protozoan parasite that
infects the small intestine of a wide range of vertebrate hosts. This study
aimed to determine whether tracing of G. duodenalis isolates by current
genetic typing tools is possible using an exemplary set of samples from
infected cattle, buffalo and children from the Ismailia province, Egypt.
Method A total of 804 fecal samples from ruminant animals was collected from
191 herds and 165 samples from diarrheal children below the age of 10 years.
Parasites were detected in these samples using the copro-antigen RIDA®QUICK
test and by real-time PCR. Samples were then genetically characterized based
on the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes.
Results The prevalence of G. duodenalis was 53% in ruminants and 21% in
symptomatic children and infection was not positively correlated with
diarrheal symptoms. Sequence typing analysis confirmed predominance of B-type
sequences (>67%) in humans and E-type sequences (>81%) in ruminants over
A-type sequences. For 39 samples the complete sequence information of the
three marker gene fragments could be derived. Integration of the concatenated
sequence information of the three marker gene fragments with the spatial data
of the respective sample revealed that identical or near identical (only up to
1 out of 1358 bp different) concatenated sequencing types were spatially
related in 4 out of 5 cases. Conclusion The risk of zoonotic infection
emanating from ruminants even in high prevalence areas is negligible. Genetic
characterization indicated a predominant anthropogenic cycle of infection
within the pediatric population studied. Integration of sequence typing data
with information on geographic origins of samples allows parasite sub-
population tracing using current typing tools
Epidemiology of Giardia duodenalis infection in ruminant livestock and children in the Ismailia province of Egypt: insights by genetic characterization
Background: Giardia duodenalis is a common flagellated protozoan parasite that infects the small intestine of a wide range of vertebrate hosts. This study aimed to determine whether tracing of G. duodenalis isolates by current genetic typing tools is possible using an exemplary set of samples from infected cattle, buffalo and children from the Ismailia province, Egypt. Method: A total of 804 fecal samples from ruminant animals was collected from 191 herds and 165 samples from diarrheal children below the age of 10 years. Parasites were detected in these samples using the copro-antigen RIDA®QUICK test and by real-time PCR. Samples were then genetically characterized based on the triosephosphate isomerase, glutamate dehydrogenase and β-giardin genes. Results: The prevalence of G. duodenalis was 53% in ruminants and 21% in symptomatic children and infection was not positively correlated with diarrheal symptoms. Sequence typing analysis confirmed predominance of B-type sequences (>67%) in humans and E-type sequences (>81%) in ruminants over A-type sequences. For 39 samples the complete sequence information of the three marker gene fragments could be derived. Integration of the concatenated sequence information of the three marker gene fragments with the spatial data of the respective sample revealed that identical or near identical (only up to 1 out of 1358 bp different) concatenated sequencing types were spatially related in 4 out of 5 cases. Conclusion: The risk of zoonotic infection emanating from ruminants even in high prevalence areas is negligible. Genetic characterization indicated a predominant anthropogenic cycle of infection within the pediatric population studied. Integration of sequence typing data with information on geographic origins of samples allows parasite sub-population tracing using current typing tools
Percolation properties of non-ideal gas
We estimate locations of the regions of the percolation and of the
non-percolation in the plane : the Poisson rate -- the inverse
temperature, for interacted particle systems in finite dimension Euclidean
spaces. Our results about the percolation and about the non-percolation are
obtained under different assumptions. The intersection of two groups of the
assumptions reduces the results to two dimension Euclidean space, , and
to a potential function of the interactions having a hard core.
The technics for the percolation proof is based on a contour method which is
applied to a discretization of the Euclidean space. The technics for the
non-percolation proof is based on the coupling of the Gibbs field with a
branching process.Comment: 28 pages, 3 figure
miR-1269 promotes metastasis and forms a positive feedback loop with TGF-β
As patient survival drops precipitously from early-stage cancers to late-stage and metastatic cancers, microRNAs that promote relapse and metastasis can serve as prognostic and predictive markers as well as therapeutic targets for chemoprevention. Here we show that miR-1269a promotes colorectal cancer (CRC) metastasis and forms a positive feedback loop with TGF-β signalling. miR-1269a is upregulated in late-stage CRCs, and long-term monitoring of 100 stage II CRC patients revealed that miR-1269a expression in their surgically removed primary tumours is strongly associated with risk of CRC relapse and metastasis. Consistent with clinical observations, miR-1269a significantly increases the ability of CRC cells to invade and metastasize in vivo. TGF-β activates miR-1269 via Sox4, while miR-1269a enhances TGF-β signalling by targeting Smad7 and HOXD10, hence forming a positive feedback loop. Our findings suggest that miR-1269a is a potential marker to inform adjuvant chemotherapy decisions for CRC patients and a potential therapeutic target to deter metastasis
Palaeozoic giant dragonfies were hawker predators
The largest insects to have ever lived were the giant meganeurids of the Late Palaeozoic, ancient
stem relatives of our modern dragonfies. With wingspans up to 71cm, these iconic insects have been
the subject of varied documentaries on Palaeozoic life, depicting them as patrolling for prey through
coal swamp forests amid giant lycopsids, and cordaites. Such reconstructions are speculative as few
defnitive details of giant dragonfy biology are known. Most specimens of giant dragonfies are known
from wings or isolated elements, but Meganeurites gracilipes preserves critical body structures, most
notably those of the head. Here we show that it is unlikely it thrived in densely forested environments
where its elongate wings would have become easily damaged. Instead, the species lived in more
open habitats and possessed greatly enlarged compound eyes. These were dorsally hypertrophied, a
specialization for long-distance vision above the animal in fight, a trait convergent with modern hawker
dragonfies. Sturdy mandibles with acute teeth, strong spines on tibiae and tarsi, and a pronounced
thoracic skewness are identical to those specializations used by dragonfies in capturing prey while
in fight. The Palaeozoic Odonatoptera thus exhibited considerable morphological specializations
associated with behaviours attributable to ‘hawkers’ or ‘perchers’ among extant Odonata.This work benefted from a grant of the French ‘Agence Nationale de la Recherche’ via the program ‘Investissements d’avenir’ (ANR-11-INBS-0004-RECOLNAT)JP and MP gratefully acknowledge research support from the Grant Agency of the Czech Republic No. 18-03118 SThe work of MSE was supported by US National Science Foundation grant DEB-114416
Reversible Fluorescence Photoswitching in DNA
[Image: see text] We describe the engineering of reversible fluorescence photoswitching in DNA with high-density substitution, and its applications in advanced fluorescence microscopy methods. High-density labeling of DNA with cyanine dyes can be achieved by polymerase chain reaction using a modified DNA polymerase that has been evolved to efficiently incorporate Cy3- and Cy5-labeled cytosine base analogues into double-stranded DNA. The resulting biopolymer, “CyDNA”, displays hundreds of fluorophores per DNA strand and is strongly colored and highly fluorescent, although previous observations suggest that fluorescence quenching at such high density might be a concern, especially for Cy5. Herein, we first investigate the mechanisms of fluorescence quenching in CyDNA and we suggest that two different mechanisms, aggregate formation and resonance energy transfer, are responsible for fluorescence quenching at high labeling densities. Moreover, we have been able to re-engineer CyDNA into a reversible fluorescence photoswitchable biopolymer by using the properties of the Cy3–Cy5 pair. This novel biopolymer constitutes a new class of photoactive DNA-based nanomaterial and is of great interest for advanced microscopy applications. We show that reversible fluorescence photoswitching in CyDNA can be exploited in optical lock-in detection imaging. It also lays the foundations for improved and sequence-specific super-resolution fluorescence microscopy of DNA
Effects of Shinrin-yoku Retreat on Mental Health: A Pilot Study in Fukushima, Japan
Shinrin-yoku (forest bathing) is a cost-effective healing practice that has recently attracted the interest of social scientists who have attributed it, in part, to mental health benefits. Japanese university students suffer from high rates of mental health problems, and the number of suicides remain high despite the total number of suicides in Japan decreasing. Effective mental health approaches which increase mental wellbeing and self-compassion, and reduce associated deficits, such as loneliness, are sought after for Japanese students, however healthful treatment has not been identified to date. Accordingly, this pre-post pilot study evaluated the levels of mental wellbeing, self-compassion, and loneliness among 25 Japanese undergraduate students who participated in a three-day shinrin-yoku retreat in Fukushima. Measurements were taken prior, straight after, and two weeks-post intervention. One-way ANOVA with Tukey post hoc analysis revealed that the mean scores of self-compassion, common humanity, and mindfulness increased statistically significantly from pre-retreat to follow-up. The mean scores of mental wellbeing and loneliness did not statistically significantly change. The positive effects on self-compassion indicate that shinrin-yoku retreat should be evaluated within a larger sample and in a shorter time frame to establish optimal shinrin-yoku parameters in this arena.N/
Effectiveness of self-compassion related therapies:A systematic review and meta-analysis
This systematic review and meta-analysis investigated whether self-compassion-related therapies, including compassion-focussed therapy, mindfulness-based cognitive therapy and acceptance and commitment therapy, are effective in promoting self-compassion and reducing psychopathology in clinical and subclinical populations. A total of 22 randomised controlled trials met inclusion criteria, with data from up to 1172 individuals included in each quantitative analysis. Effect sizes were the standardised difference in change scores between intervention and control groups. Results indicated that self-compassion-related therapies produced greater improvements in all three outcomes examined: self-compassion (g = 0.52, 95% CIs [0.32, 0.71]), anxiety (g = 0.46, 95% CIs [0.25, 0.66]) and depressive symptoms (g = 0.40, 95% CIs [0.23, 0.57]). However, when analysis was restricted to studies that compared self-compassion-related therapies to active control conditions, change scores were not significantly different between the intervention and control groups for any of the outcomes. Patient status (clinical vs. subclinical) and type of therapy (explicitly compassion-based vs. other compassion-related therapies, e.g. mindfulness) were not moderators of outcome. There was some evidence that self-compassion-related therapies brought about greater improvements in the negative than the positive subscales of the Self-Compassion Scale, although a statistical comparison was not possible. The methodological quality of studies was generally good, although risk of performance bias due to a lack of blinding of participants and therapists was a concern. A narrative synthesis found that changes in self-compassion and psychopathology were correlated in several studies, but this relationship was observed in both intervention and control groups. Overall, this review presents evidence that third-wave therapies bring about improvements in self-compassion and psychopathology, although not over and beyond other interventions
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