377 research outputs found
Coordinated balancing of muscle oxidative metabolism through PGC-1α increases metabolic flexibility and preserves insulin sensitivity
The peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) enhances oxidative metabolism in skeletal muscle. Excessive lipid oxidation and electron transport chain activity can, however, lead to the accumulation of harmful metabolites and impair glucose homeostasis. Here, we investigated the effect of over-expression of PGC-1α on metabolic control and generation of insulin desensitizing agents in extensor digitorum longus (EDL), a muscle that exhibits low levels of PGC-1α in the untrained state and minimally relies on oxidative metabolism. We demonstrate that PGC-1α induces a strictly balanced substrate oxidation in EDL by concomitantly promoting the transcription of activators and inhibitors of lipid oxidation. Moreover, we show that PGC-1α enhances the potential to uncouple oxidative phosphorylation. Thereby, PGC-1α boosts elevated, yet tightly regulated oxidative metabolism devoid of side products that are detrimental for glucose homeostasis. Accordingly, PI3K activity, an early phase marker for insulin resistance, is preserved in EDL muscle. Our findings suggest that PGC-1α coordinately coactivates the simultaneous transcription of gene clusters implicated in the positive and negative regulation of oxidative metabolism and thereby increases metabolic flexibility. Thus, in mice fed a normal chow diet, over-expression of PGC-1α does not alter insulin sensitivity and the metabolic adaptations elicited by PGC-1α mimic the beneficial effects of endurance training on muscle metabolism in this context
Interactions of local climatic, biotic and hydrogeochemical processes facilitate phosphorus dynamics along an Everglades forest-marsh gradient
Ecosystem nutrient cycling is often complex because nutrient dynamics within and between systems are mediated by the interaction of biological and geochemical conditions operating at different temporal and spatial scales. Vegetated patches in semiarid and wetland landscapes have been shown to exemplify some of these patterns and processes. We investigated biological and geochemical factors suggested to contribute to phosphorus (P) movement and availability along a forest-marsh gradient in an Everglades tree island. Our study illustrated processes that are consistent with the chemohydrodynamic nutrient (CHNT) hypothesis and the trigger-transfer, pulse-reserve (TTPR) model developed for semiarid systems. Comparison with the TTPR model was constructive as it elaborated several significant patterns and processes of the tree island ecosystem including: (1) concentration of the limiting resource (P) in the source patch (High Head which constitutes the reserve) compared with the resource-poor landscape, (2) soil zone calcite precipitation requiring strong seasonality for evapotranspiration to promote conditions for secondary soil development and calcium phosphate reprecipitation, (3) rewetting of previously dry soils by early wet season precipitation events, and (4) antecedent conditions of the source patch, including landscape position that modulated the effect of the precipitation trigger. Thus, our study showed how water availability drives soil water P dynamics and, potentially, stability of mineral soil P in this tree island ecosystem. In landscapes with extensive water management, these processes can be asynchronous with the seasonality of hydrologic dynamics, tipping the balance between a sink and source of a limiting nutrient
Enhanced glucose uptake via GLUT4 fuels recovery from calcium overload after ischaemia-reperfusion injury in sevoflurane- but not propofol-treated hearts
Background So far, no study has explored the effects of sevoflurane, propofol, and Intralipid on metabolic flux rates of fatty acid oxidation (FOX) and glucose oxidation (GOX) in hearts exposed to ischaemia-reperfusion. Methods Isolated paced working rat hearts were exposed to 20 min of ischaemia and 30 min of reperfusion. Peri-ischaemic sevoflurane (2 vol%) and propofol (100 µM) in the formulation of 1% Diprivan® were assessed for their effects on oxidative energy metabolism and intracellular diastolic and systolic Ca2+ concentrations. Substrate flux was measured using [3H]palmitate and [14C]glucose and [Ca2+] using indo-1AM. Western blotting was used to determine the expression of the sarcolemmal glucose transporter GLUT4 in lipid rafts. Biochemical analyses of nucleotides, ceramides, and 32 acylcarnitines were also performed. Results Sevoflurane, but not propofol, improved the recovery of left ventricular work (P=0.008) and myocardial efficiency (P=0.008) compared with untreated ischaemic hearts. This functional improvement was accompanied by reduced increases in post-ischaemic diastolic and systolic intracellular Ca2+ concentrations (P=0.008). Sevoflurane, but not propofol, increased GOX (P=0.009) and decreased FOX (P=0.019) in hearts exposed to ischaemia-reperfusion. GLUT4 expression was markedly increased in lipid rafts of sevoflurane-treated hearts (P=0.016). Increased GOX closely correlated with reduced Ca2+ overload. Intralipid alone decreased energy charge and increased long-chain and hydroxyacylcarnitine tissue levels, whereas sevoflurane decreased toxic ceramide formation. Conclusions Enhanced glucose uptake via GLUT4 fuels recovery from Ca2+ overload after ischaemia-reperfusion in sevoflurane- but not propofol-treated hearts. The use of a high propofol concentration (100 µM) did not result in similar protectio
On the Discovery of Monocular Rivalry by Tscherning in 1898:Translation and Review
Monocular rivalry was named by Breese in 1899. He made prolonged observation of superimposed orthogonal gratings; they fluctuated in clarity with either one or the other grating occasionally being visible alone. A year earlier, Tscherning observed similar fluctuations with a grid of vertical and horizontal lines and with other stimuli; we draw attention to his prior account. Monocular rivalry has since been shown to occur with a wide variety of superimposed patterns with several independent rediscoveries of it. We also argue that Helmholtz described some phenomenon other than monocular rivalry in 1867
Molecular cloning of a major human gall bladder mucin: complete C-terminal sequence and genomic organization of MUC5B
Uncertainty in United States coastal wetland greenhouse gas inventorying
© The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Environmental Research Letters 13 (2018): 115005, doi:10.1088/1748-9326/aae157.Coastal wetlands store carbon dioxide (CO2) and emit CO2 and methane (CH4) making them an important part of greenhouse gas (GHG) inventorying. In the contiguous United States (CONUS), a coastal wetland inventory was recently calculated by combining maps of wetland type and change with soil, biomass, and CH4 flux data from a literature review. We assess uncertainty in this developing carbon monitoring system to quantify confidence in the inventory process itself and to prioritize future research. We provide a value-added analysis by defining types and scales of uncertainty for assumptions, burial and emissions datasets, and wetland maps, simulating 10 000 iterations of a simplified version of the inventory, and performing a sensitivity analysis. Coastal wetlands were likely a source of net-CO2-equivalent (CO2e) emissions from 2006–2011. Although stable estuarine wetlands were likely a CO2e sink, this effect was counteracted by catastrophic soil losses in the Gulf Coast, and CH4 emissions from tidal freshwater wetlands. The direction and magnitude of total CONUS CO2e flux were most sensitive to uncertainty in emissions and burial data, and assumptions about how to calculate the inventory. Critical data uncertainties included CH4 emissions for stable freshwater wetlands and carbon burial rates for all coastal wetlands. Critical assumptions included the average depth of soil affected by erosion events, the method used to convert CH4 fluxes to CO2e, and the fraction of carbon lost to the atmosphere following an erosion event. The inventory was relatively insensitive to mapping uncertainties. Future versions could be improved by collecting additional data, especially the depth affected by loss events, and by better mapping salinity and inundation gradients relevant to key GHG fluxes. Social Media Abstract: US coastal wetlands were a recent and uncertain source of greenhouse gasses because of CH4 and erosion.Financial
support was provided primarily by NASA Carbon
Monitoring Systems (NNH14AY67I) and the USGS
Land Carbon Program, with additional support from
The Smithsonian Institution, The Coastal Carbon
Research Coordination Network (DEB-1655622), and
NOAA Grant: NA16NMF4630103
Greater temperature sensitivity of plant phenology at colder sites: implications for convergence across northern latitudes
Warmer temperatures are accelerating the phenology of organisms around the world. Temperature sensitivity of phenology might be greater in colder, higher latitude sites than in warmer regions, in part because small changes in temperature constitute greater relative changes in thermal balance at colder sites. To test this hypothesis, we examined up to 20 years of phenology data for 47 tundra plant species at 18 high-latitude sites along a climatic gradient. Across all species, the timing of leaf emergence and flowering was more sensitive to a given increase in summer temperature at colder than warmer high-latitude locations. A similar pattern was seen over time for the flowering phenology of a widespread species, Cassiope tetragona. These are among the first results highlighting differential phenological responses of plants across a climatic gradient and suggest the possibility of convergence in flowering times and therefore an increase in gene flow across latitudes as the climate warms
Integrated Carbon Budget Models for the Everglades Terrestrial-Coastal-Oceanic Gradient: Current Status and Needs for Inter-Site Comparisons
Recent studies suggest that coastal ecosystems can bury significantly more C than tropical forests, indicating that continued coastal development and exposure to sea level rise and storms will have global biogeochemical consequences. The Florida Coastal Everglades Long Term Ecological Research (FCE LTER) site provides an excellent subtropical system for examining carbon (C) balance because of its exposure to historical changes in freshwater distribution and sea level rise and its history of significant long-term carbon-cycling studies. FCE LTER scientists used net ecosystem C balance and net ecosystem exchange data to estimate C budgets for riverine mangrove, freshwater marsh, and seagrass meadows, providing insights into the magnitude of C accumulation and lateral aquatic C transport. Rates of net C production in the riverine mangrove forest exceeded those reported for many tropical systems, including terrestrial forests, but there are considerable uncertainties around those estimates due to the high potential for gain and loss of C through aquatic fluxes. C production was approximately balanced between gain and loss in Everglades marshes; however, the contribution of periphyton increases uncertainty in these estimates. Moreover, while the approaches used for these initial estimates were informative, a resolved approach for addressing areas of uncertainty is critically needed for coastal wetland ecosystems. Once resolved, these C balance estimates, in conjunction with an understanding of drivers and key ecosystem feedbacks, can inform cross-system studies of ecosystem response to long-term changes in climate, hydrologic management, and other land use along coastlines
The tundra phenology database: more than two decades of tundra phenology responses to climate change
Observations of changes in phenology have provided some of the strongest
signals of the effects of climate change on terrestrial ecosystems. The International
Tundra Experiment (ITEX), initiated in the early 1990s, established a common protocol to
measure plant phenology in tundra study areas across the globe. Today, this valuable collection
of phenology measurements depicts the responses of plants at the colder extremes of
our planet to experimental and ambient changes in temperature over the past decades.
The database contains 150 434 phenology observations of 278 plant species taken at 28 study
areas for periods of 1\u201326 years. Here we describe the full data set to increase the
visibility and use of these data in global analyses and to invite phenology data contributions
from underrepresented tundra locations. Portions of this tundra phenology database have
been used in three recent syntheses, some data sets are expanded, others are from entirely
new study areas, and the entirety of these data are now available at the Polar Data Catalogue
(https://doi.org/10.21963/13215)
The succession pattern of soil microbial communities and its relationship with tobacco bacterial wilt
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