Review of robust measurement of phosphorus in river water: sampling, storage, fractionation and sensitivity

International audienceThis paper reviews current knowledge on sampling, storage and analysis of phosphorus (P) in river waters. Potential sensitivity of rivers with different physical, chemical and biological characteristics (trophic status, turbidity, flow regime, matrix chemistry) is examined in terms of errors associated with sampling, sample preparation, storage, contamination, interference and analytical errors. Key issues identified include: The need to tailor analytical reagents and concentrations to take into account the characteristics of the sample matrix. The effects of matrix interference on the colorimetric analysis. The influence of variable rates of phospho-molybdenum blue colour formation. The differing responses of river waters to physical and chemical conditions of storage. The higher sensitivities of samples with low P concentrations to storage and analytical errors. Given high variability of river water characteristics in space and time, no single standardised methodology for sampling, storage and analysis of P in rivers can be offered. ?Good Practice' guidelines are suggested, which recommend that protocols for sampling, storage and analysis of river water for P is based on thorough site-specific method testing and assessment of P stability on storage. For wider sampling programmes at the regional/national scale where intensive site-specific method and stability testing are not feasible, ?Precautionary Practice' guidelines are suggested. The study highlights key areas requiring further investigation for improving methodological rigour. Keywords: phosphorus, orthophosphate, soluble reactive, particulate, colorimetry, stability, sensitivity, analytical error, storage, sampling, filtration, preservative, fractionation, digestio

Review of robust measurement of phosphorus in river water: sampling, storage, fractionation and sensitivity

This paper reviews current knowledge on sampling, storage and analysis of phosphorus (P) in river waters. Potential sensitivity of rivers with different physical, chemical and biological characteristics (trophic status, turbidity, flow regime, matrix chemistry) is examined in terms of errors associated with sampling, sample preparation, storage, contamination, interference and analytical errors. Key issues identified include: The need to tailor analytical reagents and concentrations to take into account the characteristics of the sample matrix. The effects of matrix interference on the colorimetric analysis. The influence of variable rates of phospho-molybdenum blue colour formation. The differing responses of river waters to physical and chemical conditions of storage. The higher sensitivities of samples with low P concentrations to storage and analytical errors. Given high variability of river water characteristics in space and time, no single standardised methodology for sampling, storage and analysis of P in rivers can be offered. ‘Good Practice’ guidelines are suggested, which recommend that protocols for sampling, storage and analysis of river water for P is based on thorough site-specific method testing and assessment of P stability on storage. For wider sampling programmes at the regional/national scale where intensive site-specific method and stability testing are not feasible, ‘Precautionary Practice’ guidelines are suggested. The study highlights key areas requiring further investigation for improving methodological rigour. Keywords: phosphorus, orthophosphate, soluble reactive, particulate, colorimetry, stability, sensitivity, analytical error, storage, sampling, filtration, preservative, fractionation, digestio

Effects of neo-adjuvant chemotherapy for oesophago-gastric cancer on neuro-muscular gastric function

Delayed gastric emptying symptoms are often reported after chemotherapy. This study aims to characterise the effects of chemotherapy on gastric neuro-muscular function. Patients undergoing elective surgery for oesophago-gastric cancer were recruited. Acetylcholinesterase, nNOS, ghrelin receptor and motilin expressions were studied in gastric sections from patients receiving no chemotherapy (n = 3) or oesophageal (n = 2) or gastric (n = 2) chemotherapy. A scoring system quantified staining intensity (0–3; no staining to strong). Stomach sections were separately suspended in tissue baths for electrical field stimulation (EFS) and exposure to erythromycin or carbachol; three patients had no chemotherapy; four completed cisplatin-based chemotherapy within 6 weeks prior to surgery. AChE expression was markedly decreased after chemotherapy (scores 2.3 ± 0.7, 0.5 ± 0.2 and 0 ± 0 in non-chemotherapy, oesophageal- and gastric-chemotherapy groups (p < 0.03 each) respectively. Ghrelin receptor and motilin expression tended to increase (ghrelin: 0.7 ± 0.4 vs 2.0 ± 0.4 and 1.2 ± 0.2 respectively; p = 0.04 and p = 0.2; motilin: 0.7 ± 0.5 vs 2.2 ± 0.5 and 2.0 ± 0.7; p = 0.06 and p = 0.16). Maximal contraction to carbachol was 3.7 ± 0.7 g and 1.9 ± 0.8 g (longitudinal muscle) and 3.4 ± 0.4 g and 1.6 ± 0.6 (circular) in non-chemotherapy and chemotherapy tissues respectively (p < 0.05 each). There were loss of AChE and reduction in contractility to carbachol. The tendency for ghrelin receptors to increase suggests an attempt to upregulate compensating systems. Our study offers a mechanism by which chemotherapy markedly alters neuro-muscular gastric function

'Anyone but England'? Exploring Anti-English Sentiment as Part of Scottish National Identity in Sport.

This article explores the reasons behind the expression of anti-English sentiment by Scots in relation to both sporting and wider social contexts, whilst also considering the impact of migration to England on the attitudes expressed by members of the Scottish diaspora. Drawing upon the conceptual framework of ‘narrative identity’ proposed elsewhere, data was generated through semi-structured interviews which focused upon the ‘ontological’ and ‘public’ narratives of Scottish identity as expressed by Scots living in England. The findings demonstrate the influence of a wide range of personal, social, historical and political factors in highlighting the cleavage between Scotland and England within the context of sport and society, allowing for a more nuanced understanding of the complex relationship between these two nations in the context of sporting rivalry. This relationship is argued to be heavily influenced by the existence of an ‘underdog mentality’-style grand ‘public narrative’ for Scots in relation to their English neighbours, based on perceived differences in economic and sporting resources between the two countries. This ‘underdog mentality’ therefore acts as a legitimating force for the expression of anti-English sentiment in both a sporting and wider social context as part of some individuals’ ‘ontological narrative’, although the extent of such sentiment was found to vary significantly between individuals and contrasting contexts

Sport, social change and the public intellectual

This article argues that the role of the public intellectual in sport is desperately needed. The research for the article draws upon key interviews and newspaper reports. The paper examines three questions: (i) What is the role of the public intellectual in sport? (ii) Do we wish to encourage the role of the public intellectual in sport? (iii) How does one balance the objective of challenging unseen silences in sport with its potential transformative capacity to produce change (or at least be a resource for hope) in many communities. The challenge is for today’s sociologists of sport and others not to accept the narrow job description of the academic but instead to ensure that the social study of sport is one of these very public, visible forms of activity and engagement

Future agriculture with minimized phosphorus losses to waters: research needs and direction

The series of papers in this issue of AMBIO represent technical presentations made at the 7th International Phosphorus Workshop (IPW7), held in September, 2013 in Uppsala, Sweden. At that meeting, the 150 delegates were involved in round table discussions on major, predetermined themes facing the management of agricultural phosphorus (P) for optimum production goals with minimal water quality impairment. The six themes were (1) P management in a changing world; (2) transport pathways of P from soil to water; (3) monitoring, modeling, and communication; (4) importance of manure and agricultural production systems for P management; (5) identification of appropriate mitigation measures for reduction of P loss; and (6) implementation of mitigation strategies to reduce P loss. This paper details the major challenges and research needs that were identified for each theme and identifies a future roadmap for catchment management that cost-effectively minimizes P loss from agricultural activities

Sport for Yes? The role of sporting issues in pro-independence political discourse during the Scottish independence referendum campaign

This article critically considers the extent to which sporting issues were harnessed by pro-independence political campaigners during the Scottish independence referendum campaign. Developments such as the inclusion of sport within the Scottish Government’s White Paper on Scottish independence, the establishment of the ‘Working Group on Scottish Sport’ and the establishment of the ‘Sport for Yes’ campaign group demonstrate the harnessing of sporting issues as an additional, if somewhat peripheral, debate point in the referendum campaigns (Lafferty 2014, Scottish Government 2013, Working Group on Scottish Sport and Scottish Government 2013, 2014). The latter of these developments, the establishment of the ‘Sport for Yes’ campaign group, is of particular interest, offering evidence of the explicit political mobilisation of past and present athletes in support of the ‘Yes Scotland’ pro-independence campaign. The use of sport within pro-independence political discourse is therefore scrutinised, drawing upon the principles of critical discourse analysis to explore the ideological assumptions underpinning the discursive representation of sport in relation to Scottish independence