Regulatory T cells in melanoma revisited by a computational clustering of FOXP3+ T cell subpopulations

CD4+ T cells that express the transcription factor FOXP3 (FOXP3+ T cells) are commonly regarded as immunosuppressive regulatory T cells (Treg). FOXP3+ T cells are reported to be increased in tumour-bearing patients or animals, and considered to suppress anti-tumour immunity, but the evidence is often contradictory. In addition, accumulating evidence indicates that FOXP3 is induced by antigenic stimulation, and that some non-Treg FOXP3+ T cells, especially memory-phenotype FOXP3low cells, produce proinflammatory cytokines. Accordingly, the subclassification of FOXP3+ T cells is fundamental for revealing the significance of FOXP3+ T cells in tumour immunity, but the arbitrariness and complexity of manual gating have complicated the issue. Here we report a computational method to automatically identify and classify FOXP3+ T cells into subsets using clustering algorithms. By analysing flow cytometric data of melanoma patients, the proposed method showed that the FOXP3+ subpopulation that had relatively high FOXP3, CD45RO, and CD25 expressions was increased in melanoma patients, whereas manual gating did not produce significant results on the FOXP3+ subpopulations. Interestingly, the computationally-identified FOXP3+ subpopulation included not only classical FOXP3high Treg but also memory-phenotype FOXP3low cells by manual gating. Furthermore, the proposed method successfully analysed an independent dataset, showing that the same FOXP3+ subpopulation was increased in melanoma patients, validating the method. Collectively, the proposed method successfully captured an important feature of melanoma without relying on the existing criteria of FOXP3+ T cells, revealing a hidden association between the T cell profile and melanoma, and providing new insights into FOXP3+ T cells and Treg

Negative illness perceptions are related to poorer health‐related quality of life among thyroid cancer survivors:Results from the PROFILES registry

Background Differentiated thyroid cancer (DTC) reports a poorer health‐related quality of life (HRQoL) than a norm population. Patients' illness perceptions are modifiable and known associates of HRQoL in other cancers. The aim was to examine the relationship between illness perceptions and HRQoL among DTC survivors. Methods DTC survivors registered in the Netherlands Cancer Registry diagnosed between 1990 and 2008, received a survey on illness perceptions (Brief‐Illness Perception Questionnaire; B‐IPQ) and HRQoL (European Organisation for Research and Treatment of Cancer, Quality of Life Questionnaire‐Core 30; EORTC QLQ‐C30). Multiple regression analyses were conducted investigating the relation between illness perceptions and HRQoL, while controlling for sociodemographic and clinical characteristics. Results Two hundred and eighty‐four DTC survivors were included. DTC survivors who believed their illness had many negative consequences; who perceived their illness as controllable by treatment; who had strong beliefs symptoms could be attributed to their illness; and who had strong beliefs their illness causes negative emotions, reported a poorer HRQoL. Conclusions Stronger negative illness perceptions are related to a poorer HRQoL among DTC survivors

The Rotation of Sub-Populations in omega Centauri

We present the first result of the Ital-FLAMES survey of red giant branch (RGB) stars in omega Cen. Radial velocities with a precision of ~0.5 km/s are presented for 650 members of omega Cen observed with FLAMES-Giraffe at the Very Large Telescope. We found that stars belonging to the metal -poor (RGB-MP), metal-intemediate (RGB-MInt) and metal-rich (RGB-a) sub -populations of Omega Cen are all compatible with having the same rotational pattern. Our results appear to contradict past findings by Norris et al., who could not detect any rotational signature for metal -rich stars. The slightly higher precision of the present measurements and the much larger sample size, especially for the metal-richer stars, appear as the most likely explanation for this discrepancy. The result presented here weakens the body of evidence in favour of a merger event in the past history of omega Cen.Comment: 5 pages, 3 fiures, electronic table can be obtained from E. Pancino. Accepted for publication in ApJ Letter

Place et rôle du riz pluvial dans les systèmes de production du lac Alaotra Les systèmes SCV (semis direct sur couverture végétale permanente) : une alternative viable pour une agriculture pluviale durable

La région de l'Alaotra repose sur un plateau situé à 750 mètres d'altitude avec au cœur de la plaine le lac Alaotra. La surface cultivée en riziculture est estimée à 148 500 ha en 2000 (FAO/UPDR, 2000), dont 75-80 000 ha dans la cuvette du Lac et plus de 65-70 000 ha au sud et dans les zones en périphérie, qui constitue 10% de la surface rizicole nationale pour seulement 4% des riziculteurs malgaches. Cette région réalise 33% de la valeur ajoutée de l'ensemble de la filière nationale et 15% de la richesse générée par le secteur riz. Le climat irrégulier constitue une contrainte majeure pour tous les agriculteurs du lac Alaotra. Les relations agriculture-élevage sont au cœur de la problématique de développement et d'évolution des exploitations agricoles.Ainsi, avec la saturation des rizières irrigables ou à Mauvaise Maîtrise de l'Eau (RMME), la colonisation agricole des tanety anciennement dévolus aux pâturages extensifs depuis les années 1980 s'est accélérer, induisant la perturbation des activités d'élevage bovin traditionnel extensif à objectif de capitalisation qui évoluent aujourd'hui vers un élevage productif (embouche et production laitière) ou de trait. Le diagnostic réalisé en 2007 a mis en évidence 7 types d'exploitations agricoles différents. Les différents systèmes de riziculture pluviale se retrouvent sur les plateaux sommitaux et les pentes des collines (en rotation jachère culture sur les sols très pauvres), les bas de pente et les baiboho, en rotation avec d'autres cultures pluviales (maïs, manioc, pois de terre...) avec ou sans jachère, ou en système SCV depuis leur introduction au début des années 2000. L'introduction de variétés composites poly-aptitudes de type Sebota a pu lever une contrainte majeure des zones ni totalement irriguées ni totalement pluviales mais intermédiaires dans les RMME (riziculture pluviale partiellement irriguée, selon l'accès à l'eau et les caractéristiques climatiques de la saison), Les systèmes SCV mis au point sur baiboho avec des successions culturales de type riz de saison / haricot de contre saison - maïs de saison ou riz de saison puis vesce de contre saison associée ou non à des cultures maraîchères montrent également un niveau de production et de sécurité par rapport aux aléas climatiques très proche voire supérieurs à ceux obtenus en riziculture irriguée. La part du riz pluvial dans la formation du revenu et la sécurité alimentaire a été analysée pour chaque type d'exploitation et varie de 20 à 30 % du revenu riz total (avant autoconsommation), et se révèle croissante en fonction du rapport tanety / rizière dans l'exploitation. Ainsi, sur certaines zones, le riz pluvial semble avoir de beaux jours devant lui

Synthesising, using, and correcting for telluric features in high-resolution astronomical spectra

We present a technique to synthesise telluric absorption and emission features both for in-situ wavelength calibration and for their removal from astronomical spectra. While the presented technique is applicable for a wide variety of optical and infrared spectra, we concentrate in this paper on selected high-resolution near-infrared spectra obtained with the CRIRES spectrograph to demonstrate its performance and limitation. We find that synthetic spectra reproduce telluric absorption features to about 2%, even close to saturated line cores. Thus, synthetic telluric spectra could be used to replace the observation of telluric standard stars, saving valuable observing time. This technique also provides a precise in-situ wavelength calibration, especially useful for high-resolution near-infrared spectra in the absence of other calibration sources.Comment: 11 pages, 11 figures, accepted for publication in A&A (updated version

Charge transfer electrostatic model of compositional order in perovskite alloys

We introduce an electrostatic model including charge transfer, which is shown to account for the observed B-site ordering in Pb-based perovskite alloys. The model allows charge transfer between A-sites and is a generalization of Bellaiche and Vanderbilt's purely electrostatic model. The large covalency of Pb^{2+} compared to Ba^{2+} is modeled by an environment dependent effective A-site charge. Monte Carlo simulations of this model successfully reproduce the long range compositional order of both Pb-based and Ba-based complex A(BB^{'}B^{''})O_3 perovskite alloys. The models are also extended to study systems with A-site and B-site doping, such as (Na_{1/2}La_{1/2})(Mg_{1/3}Nb_{2/3})O_3, (Ba_{1-x}La_{x})(Mg_{(1+x)/3}Nb_{(2-x)/3})O_3 and (Pb_{1-x}La_{x})(Mg_{(1+x)/3}Ta_{(2-x)/3})O_3. General trends are reproduced by purely electrostatic interactions, and charge transfer effects indicate that local structural relaxations can tip the balance between different B-site orderings in Pb based materials.Comment: 15 pages, 6 figure

Water in Comet 2/2003 K4 (LINEAR) with Spitzer

We present sensitive 5.5 to 7.6 micron spectra of comet C/2003 K4 (LINEAR) obtained on 16 July 2004 (r_{h} = 1.760 AU, Delta_{Spitzer} = 1.409 AU, phase angle 35.4 degrees) with the Spitzer Space Telescope. The nu_{2} vibrational band of water is detected with a high signal-to-noise ratio (> 50). Model fitting to the best spectrum yields a water ortho-to-para ratio of 2.47 +/- 0.27, which corresponds to a spin temperature of 28.5^{+6.5}_{-3.5} K. Spectra acquired at different offset positions show that the rotational temperature decreases with increasing distance from the nucleus, which is consistent with evolution from thermal to fluorescence equilibrium. The inferred water production rate is (2.43 +/- 0.25) \times 10^{29} molec. s^{-1}. The spectra do not show any evidence for emission from PAHs and carbonate minerals, in contrast to results reported for comets 9P/Tempel 1 and C/1995 O1 (Hale-Bopp). However, residual emission is observed near 7.3 micron the origin of which remains unidentified.Comment: 33 pages, including 11 figures, 2 tables, ApJ 2007 accepte

Electrostatic model of atomic ordering in complex perovskite alloys

We present a simple ionic model which successfully reproduces the various types of compositional long-range order observed in a large class of complex insulating perovskite alloys. The model assumes that the driving mechanism responsible for the ordering is simply the electrostatic interaction between the different ionic species. A possible new explanation for the anomalous long-range order observed in some Pb relaxor alloys, involving the proposed existence of a small amount of Pb^4+ on the B sublattice, is suggested by an analysis of the model.Comment: 4 pages, two-column style with 1 postscript figure embedded. Uses REVTEX and epsf macros. Also available at http://www.physics.rutgers.edu/~dhv/preprints/index.html#lb_orde

Assessing measurement invariance in the EORTC QLQ-C30

PURPOSE: We aimed to investigate measurement invariance (MI) in the European Organisation for research and treatment of cancer quality of life questionnaire core 30 (EORTC QLQ-C30) in a heterogeneous sample of patients with cancer. METHODS: Data from 12 studies within the PROFILES registry were used for secondary analyses (n = 7007). We tested MI by successive restrictions on thresholds, loadings, and intercepts across subgroups based on primary cancer sites, age, sex, time since diagnosis, and life stage, using multigroup confirmatory factor analysis (MGCFA) for ordered categorical measures. We also evaluated the impact of potentially miss-specified parameter equality across groups on latent factor means by releasing threshold and loading equality constraints for each item at a time. RESULTS: Results showed that the highest level of MI (invariance of thresholds, loadings, and intercepts) was found across groups based on time since diagnosis and life stage and to a lesser extent across groups based on sex, age, and primary tumor site. On item level, however, changes in the item’s associated factor means were relatively small and in most cases canceled each other out to some extent. CONCLUSIONS: Given only a few instances of non-invariance in our study, there is reason to be confident that valid conclusions can be drawn from between-group comparisons of QLQ-C30 latent means as operationalized in our study. Nonetheless, further research into MI between other subgroups for the QLQ-C30 (i.e., treatment effects and ethnicity) is warranted. We stress the importance of including MI evaluations in the development and validation of measurement instruments. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11136-021-02961-8