269 research outputs found
Stacking order dynamic in the quasi-two-dimensional dichalcogenide 1T-TaS probed with MeV ultrafast electron diffraction
Transitions between different charge density wave (CDW) states in
quasi-two-dimensional materials may be accompanied also by changes in the
inter-layer stacking of the CDW. Using MeV ultrafast electron diffraction, the
out-of-plane stacking order dynamics in the quasi-two-dimensional
dichalcogenide 1T-TaS is investigated for the first time. From the
intensity of the CDW satellites aligned around the commensurate = 1/6
characteristic stacking order, it is found out that this phase disappears with
a 0.5 ps time constant. Simultaneously, in the same experiment, the emergence
of the incommensurate phase, with a slightly slower 2.0 ps time constant, is
determined from the intensity of the CDW satellites aligned around the
incommensurate = 1/3 characteristic stacking order. These results might be
of relevance in understanding the metallic character of the laser-induced
metastable "hidden" state recently discovered in this compound
Spin configurations in Co2FeAl0.4Si0.6 Heusler alloy thin film elements
We determine experimentally the spin structure of half-metallic
Co2FeAl0.4Si0.6 Heusler alloy elements using magnetic microscopy. Following
magnetic saturation, the dominant magnetic states consist of quasi-uniform
configurations, where a strong influence from the magnetocrystalline anisotropy
is visible. Heating experiments show the stability of the spin configuration of
domain walls in confined geometries up to 800 K. The switching temperature for
the transition from transverse to vortex walls in ring elements is found to
increase with ring width, an effect attributed to structural changes and
consequent changes in magnetic anisotropy, which start to occur in the narrower
elements at lower temperatures.Comment: 4 pages, 4 figure
De Novo Missense Variants in SLC32A1 Cause a Developmental and Epileptic Encephalopathy Due to Impaired GABAergic Neurotransmission
Objective:Rare inherited missense variants inSLC32A1, the gene that encodes the vesicular gamma-aminobutyric acid(GABA) transporter, have recently been shown to cause genetic epilepsy with febrile seizures plus. We aimed to clarifyif de novo missense variants inSLC32A1can also cause epilepsy with impaired neurodevelopment.Methods:Using exome sequencing, we identified four individuals with a developmental and epileptic encephalopathyand de novo missense variants inSLC32A1. To assess causality, we performed functional evaluation of the identifiedvariants in a murine neuronal cell culture model.Results:The main phenotype comprises moderate-to-severe intellectual disability, infantile-onset epilepsy within thefirst 18 months of life, and a choreiform, dystonic, or dyskinetic movement disorder. In silico modeling and functionalanalyses reveal that three of these variants, which are located in helices that line the putative GABA transport pathway,result in reduced quantal size, consistent with impairedfilling of synaptic vesicles with GABA. The fourth variant,located in the vesicular gamma-aminobutyric acid N-terminus, does not affect quantal size, but increases presynapticrelease probability, leading to more severe synaptic depression during high-frequency stimulation. Thus, variants invesicular gamma-aminobutyric acid can impair GABAergic neurotransmission through at least two mechanisms, byaffecting synaptic vesiclefilling and by altering synaptic short-term plasticity.Interpretation:This work establishes de novo missense variants inSLC32A1as a novel cause of a developmental andepileptic encephalopathy
Magnetic switching in granular FePt layers promoted by near-field laser enhancement
Light-matter interaction at the nanoscale in magnetic materials is a topic of
intense research in view of potential applications in next-generation
high-density magnetic recording. Laser-assisted switching provides a pathway
for overcoming the material constraints of high-anisotropy and high-packing
density media, though much about the dynamics of the switching process remains
unexplored. We use ultrafast small-angle x-ray scattering at an x-ray
free-electron laser to probe the magnetic switching dynamics of FePt
nanoparticles embedded in a carbon matrix following excitation by an optical
femtosecond laser pulse. We observe that the combination of laser excitation
and applied static magnetic field, one order of magnitude smaller than the
coercive field, can overcome the magnetic anisotropy barrier between "up" and
"down" magnetization, enabling magnetization switching. This magnetic switching
is found to be inhomogeneous throughout the material, with some individual FePt
nanoparticles neither switching nor demagnetizing. The origin of this behavior
is identified as the near-field modification of the incident laser radiation
around FePt nanoparticles. The fraction of not-switching nanoparticles is
influenced by the heat flow between FePt and a heat-sink layer
Emergent dynamic chirality in a thermally driven artificial spin ratchet
Modern nanofabrication techniques have opened the possibility to create novel functional materials, whose properties transcend those of their constituent elements. In particular, tuning the magnetostatic interactions in geometrically frustrated arrangements of nanoelements called artificial spin ice1, 2 can lead to specific collective behaviour3, including emergent magnetic monopoles4, 5, charge screening6, 7 and transport8, 9, as well as magnonic response10, 11, 12. Here, we demonstrate a spin-ice-based active material in which energy is converted into unidirectional dynamics. Using X-ray photoemission electron microscopy we show that the collective rotation of the average magnetization proceeds in a unique sense during thermal relaxation. Our simulations demonstrate that this emergent chiral behaviour is driven by the topology of the magnetostatic field at the edges of the nanomagnet array, resulting in an asymmetric energy landscape. In addition, a bias field can be used to modify the sense of rotation of the average magnetization. This opens the possibility of implementing a magnetic Brownian ratchet13, 14, which may find applications in novel nanoscale devices, such as magnetic nanomotors, actuators, sensors or memory cells
Metagenomics-based proficiency test of smoked salmon spiked with a mock community
An inter-laboratory proficiency test was organized to assess the ability of participants to perform shotgun metagenomic sequencing of cold smoked salmon, experimentally spiked with a mock community composed of six bacteria, one parasite, one yeast, one DNA, and two RNA viruses. Each participant applied its in-house wet-lab workflow(s) to obtain the metagenomic dataset(s), which were then collected and analyzed using MG-RAST. A total of 27 datasets were analyzed. Sample pre-processing, DNA extraction protocol, library preparation kit, and sequencing platform, influenced the abundance of specific microorganisms of the mock community. Our results highlight that despite differences in wet-lab protocols, the reads corresponding to the mock community members spiked in the cold smoked salmon, were both detected and quantified in terms of relative abundance, in the metagenomic datasets, proving the suitability of shotgun metagenomic sequencing as a genomic tool to detect microorganisms belonging to different domains in the same food matrix. The implementation of standardized wet-lab protocols would highly facilitate the comparability of shotgun metagenomic sequencing dataset across laboratories and sectors. Moreover, there is a need for clearly defining a sequencing reads threshold, to consider pathogens as detected or undetected in a food sample
Production of Mesons in the Reaction at 3.67 GeV/c
The ratio of the total exclusive production cross sections for
and mesons has been measured in the reaction at
GeV/c. The observed ratio is
from which the exclusive
meson production cross section is determined to be
. Differential cross section
distributions have been measured. Their shape is consistent with isotropic
meson production.Comment: 14 pages, 5 figures, accepted by Phys.Lett.
Lentiviral Vpx Accessory Factor Targets VprBP/DCAF1 Substrate Adaptor for Cullin 4 E3 Ubiquitin Ligase to Enable Macrophage Infection
Vpx is a small virion-associated adaptor protein encoded by viruses of the HIV-2/SIVsm lineage of primate lentiviruses that enables these viruses to transduce monocyte-derived cells. This probably reflects the ability of Vpx to overcome an as yet uncharacterized block to an early event in the virus life cycle in these cells, but the underlying mechanism has remained elusive. Using biochemical and proteomic approaches, we have found that Vpx protein of the pathogenic SIVmac 239 strain associates with a ternary protein complex comprising DDB1 and VprBP subunits of Cullin 4–based E3 ubiquitin ligase, and DDA1, which has been implicated in the regulation of E3 catalytic activity, and that Vpx participates in the Cullin 4 E3 complex comprising VprBP. We further demonstrate that the ability of SIVmac as well as HIV-2 Vpx to interact with VprBP and its associated Cullin 4 complex is required for efficient reverse transcription of SIVmac RNA genome in primary macrophages. Strikingly, macrophages in which VprBP levels are depleted by RNA interference resist SIVmac infection. Thus, our observations reveal that Vpx interacts with both catalytic and regulatory components of the ubiquitin proteasome system and demonstrate that these interactions are critical for Vpx ability to enable efficient SIVmac replication in primary macrophages. Furthermore, they identify VprBP/DCAF1 substrate receptor for Cullin 4 E3 ubiquitin ligase and its associated protein complex as immediate downstream effector of Vpx for this function. Together, our findings suggest a model in which Vpx usurps VprBP-associated Cullin 4 ubiquitin ligase to enable efficient reverse transcription and thereby overcome a block to lentivirus replication in monocyte-derived cells, and thus provide novel insights into the underlying molecular mechanism
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