A new lab facility for measuring bidirectional reflectance/emittance distribution functions of soils and canopies

Recently, a laboratory measurement facility has been realized for assessing the anisotropic reflectance and emittance behaviour of soils, leaves and small canopies under controlled illumination conditions. The facility consists of an ASD FieldSpec 3 spectroradiometer covering the spectral range from 350 – 2500 nm at 1 nm spectral sampling interval. The spectroradiometer is deployed using a fiber optic cable with either a 1°, 8° or 25° instantaneous field of view (IFOV). These measurements can be used to assess the plant pigment (chlorophyll, xanthophyll, etc.) and non-pigment system (water, cellulose, lignin, nitrogen, etc.). The thermal emittance is measured using a NEC TH9100 Infrared Thermal Imager. It operates in a single band covering the spectral range from 8 – 14 mm with a resolution of 0.02 K. Images are 320 (H) by 240 (V) pixels with an IFOV of 1.2 mrad. A 1000 W Quartz Tungsten Halogen (QTH) lamp is used as illumination source, approximating the radiance distribution of the sun. This one is put at a fixed position during a measurement session. Multi-angular measurements are achieved by using a robotic positioning system allowing to perform either reflectance or emittance measurements over almost a complete hemisphere. The hemisphere can be sampled continuously between 0° and 80° from nadir and up to a few degrees from the hot-spot configuration (depending on the IFOV of the measurement device) for a backscattering target. Measurement distance to targets can be varied between 0.25 and 1 m, although with a distance of more than 0.6 m it is not possible to cover the full hemisphere. The goal is to infer the BRDF (bidirectional reflectance distribution function) and BTDF (bidirectional thermal distribution function) from these multi-angular measurements for various surface types (like soils, agricultural crops, small tree canopies and artificial objects) and surface roughness. The steering of the robotic arm and the reading of the spectroradiometer and the thermal camera are all fully automated

Polarizing intestinal epithelial cells electrically through Ror2

© 2014. Published by The Company of Biologists Ltd.Peer reviewedPublisher PD

Ефективність симбіотичної азотфіксації в агроценозах України

Проведено порівняльну оцінку ефективності виробничих і перспективних штамів бульбочкових бактерій колекції Південної дослідної станції ІСГМ УААН у симбіозі з сучасними сортами бобових культур. Визначені високоефективні комбінації “сорти – штами”. Показано доцільність передпосівної інокуляції насіння бобових культур на фоні ґрунтової популяції ризобій.Проведена сравнительная оценка эффективности производственных и перспективных штаммов клубеньковых бактерий коллекции Южной опытной станции ИСХМ УААН в симбиозе с современными сортами бобовых культур. Определены высокоэффективные комбинации “сорта – штаммы”. Показана целесообразность предпосевной инокуляции семян бобовых культур на фоне почвенной популяции ризобий.The comparative estimation of rhisobia strains (from the collection of the Southern Experimental Station of Institute of Agricultural Microbiology UAAS) efficiency in symbiosis with modern legume cultivars was done. Highly effective complemented „cultivars – strains” combinations have been determined. It was shown the expediency of legume seeds pre-sowing treatment on background of soil rhisobia population

Land cover classification using multi-temporal MERIS vegetation indices

The spectral, spatial, and temporal resolutions of Envisat's Medium Resolution Imaging Spectrometer (MERIS) data are attractive for regional- to global-scale land cover mapping. Moreover, two novel and operational vegetation indices derived from MERIS data have considerable potential as discriminating variables in land cover classification. Here, the potential of these two vegetation indices (the MERIS global vegetation index (MGVI), MERIS terrestrial chlorophyll index (MTCI)) was evaluated for mapping eleven broad land cover classes in Wisconsin. Data acquired in the high and low chlorophyll seasons were used to increase inter-class separability. The two vegetation indices provided a higher degree of inter-class separability than data acquired in many of the individual MERIS spectral wavebands. The most accurate landcover map (73.2%) was derived from a classification of vegetation index-derived data with a support vector machine (SVM), and was more accurate than the corresponding map derived from a classification using the data acquired in the original spectral wavebands

Cancer stem cells revisited

© 2017 Nature America, Inc., part of Springer Nature. All rights reserved. The cancer stem cell (CSC) concept was proposed four decades ago, and states that tumor growth, analogous to the renewal of healthy tissues, is fueled by small numbers of dedicated stem cells. It has gradually become clear that many tumors harbor CSCs in dedicated niches, and yet their identification and eradication has not been as obvious as was initially hoped. Recently developed lineage-tracing and cell-ablation strategies have provided insights into CSC plasticity, quiescence, renewal, and therapeutic response. Here we discuss new developments in the CSC field in relationship to changing insights into how normal stem cells maintain healthy tissues. Expectations in the field have become more realistic, and now, the first successes of therapies based on the CSC concept are emerging

Психолого-педагогічний аналіз комунікативної діяльності педагога

У статті комунікативна діяльність розглядається як структурно-функціональний компонент педагогічної діяльності вчителя, аналізується взаємозв’язок педагогічної й комунікативної задач, розкриваються особливості процесу вирішення комунікативно-педагогічної задачі.In the article communicative activities are considered as structural and functional components of the teacher’s pedagogical activities, the relationship of pedagogical and communicative tasks are analyzed, features of the solution process communicative and pedagogical tasks are revealed

Detection of the tulip breaking virus (TBV) in tulips using optical sensors

The tulip breaking virus (TBV) causes severe economic losses for countries that export tulips such as the Netherlands. Infected plants have to be removed from the field as soon as possible. There is an urgent need for a rapid and objective method of screening. In this study, four proximal optical sensing techniques for the detection of TBV in tulip plants were evaluated and compared with a visual assessment by crop experts as well as with an ELISA (enzyme immunoassay) analysis of the same plants. The optical sensor techniques used were an RGB color camera, a spectrophotometer measuring from 350 to 2500 nm, a spectral imaging camera covering a spectral range from 400 to 900 nm and a chlorophyll fluorescence imaging system that measures the photosynthetic activity. Linear discriminant classification was used to compare the results of these optical techniques and the visual assessment with the ELISA score. The spectral imaging system was the best optical technique and its error was only slightly larger than the visual assessment error. The experimental results appear to be promising, and they have led to further research to develop an autonomous robot for the detection and removal of diseased tulip plants in the open field. The application of this robot system will reduce the amount of insecticides and the considerable pressure on labor for selecting diseased plants by the crop expert. © 2010 The Author(s

WNT signaling regulates self-renewal and differentiation of prostate cancer cells with stem cell characteristics

Prostate cancer cells with stem cell characteristics were identified in human prostate cancer cell lines by their ability to form from single cells self-renewing prostaspheres in non-adherent cultures. Prostaspheres exhibited heterogeneous expression of proliferation, differentiation and stem cell-associated makers CD44, ABCG2 and CD133. Treatment with WNT inhibitors reduced both prostasphere size and self-renewal. In contrast, addition of Wnt3a caused increased prostasphere size and self-renewal, which was associated with a significant increase in nuclear Β-catenin, keratin 18, CD133 and CD44 expression. As a high proportion of LNCaP and C4-2B cancer cells express androgen receptor we determined the effect of the androgen receptor antagonist bicalutamide. Androgen receptor inhibition reduced prostasphere size and expression of PSA, but did not inhibit prostasphere formation. These effects are consistent with the androgen-independent self-renewal of cells with stem cell characteristics and the androgen-dependent proliferation of transit amplifying cells. As the canonical WNT signaling effector Β-catenin can also associate with the androgen receptor, we propose a model for tumour propagation involving a balance between WNT and androgen receptor activity. That would affect the self-renewal of a cancer cell with stem cell characteristics and drive transit amplifying cell proliferation and differentiation. In conclusion, we provide evidence that WNT activity regulates the self-renewal of prostate cancer cells with stem cell characteristics independently of androgen receptor activity. Inhibition of WNT signaling therefore has the potential to reduce the self-renewal of prostate cancer cells with stem cell characteristics and improve the therapeutic outcome.Peer reviewe

WNT signalling in prostate cancer

Genome sequencing and gene expression analyses of prostate tumours have highlighted the potential importance of genetic and epigenetic changes observed in WNT signalling pathway components in prostate tumours-particularly in the development of castration-resistant prostate cancer. WNT signalling is also important in the prostate tumour microenvironment, in which WNT proteins secreted by the tumour stroma promote resistance to therapy, and in prostate cancer stem or progenitor cells, in which WNT-β-catenin signals promote self-renewal or expansion. Preclinical studies have demonstrated the potential of inhibitors that target WNT receptor complexes at the cell membrane or that block the interaction of β-catenin with lymphoid enhancer-binding factor 1 and the androgen receptor, in preventing prostate cancer progression. Some WNT signalling inhibitors are in phase I trials, but they have yet to be tested in patients with prostate cancer

Mathematical and Statistical Techniques for Systems Medicine: The Wnt Signaling Pathway as a Case Study

The last decade has seen an explosion in models that describe phenomena in systems medicine. Such models are especially useful for studying signaling pathways, such as the Wnt pathway. In this chapter we use the Wnt pathway to showcase current mathematical and statistical techniques that enable modelers to gain insight into (models of) gene regulation, and generate testable predictions. We introduce a range of modeling frameworks, but focus on ordinary differential equation (ODE) models since they remain the most widely used approach in systems biology and medicine and continue to offer great potential. We present methods for the analysis of a single model, comprising applications of standard dynamical systems approaches such as nondimensionalization, steady state, asymptotic and sensitivity analysis, and more recent statistical and algebraic approaches to compare models with data. We present parameter estimation and model comparison techniques, focusing on Bayesian analysis and coplanarity via algebraic geometry. Our intention is that this (non exhaustive) review may serve as a useful starting point for the analysis of models in systems medicine.Comment: Submitted to 'Systems Medicine' as a book chapte