Raster image correlation spectroscopy in live cells expressing Endothelin ETA Receptor

Fluorescence spectroscopy is the most common non-radioactive technique used to study GPCR interactions with their ligands. Raster image correlation spectroscopy (RICS) exploits spatio-temporal correlation functions rather than the simple temporal correlations of conventional fluorescence correlation spectroscopy. In this paper we describe the use of RICS and the number and brightness method to determine the diffusion of a construct of endothelin ETA receptor with EGFP and the aggregation state in the cytoplasm. Our construct seems to locate mainly in the cytoplasm where it undergoes diffusion and it appears to be monomeric. Although our construct could not fully represent the native protein, we believe that the methodology we describe in this paper could be used by anyone in this field

Challenges and Opportunities of Doing Fieldwork as a Woman on Women in Guinea

The researcher with his or her multiple identities, his or her background, and habits forms part of the experience in the field and therefore influences data generation. In this chapter, Ammann reflects on how her identity shaped her field research on the women-state nexus in a Muslim West African city. She analyses how she has dealt with gender-related difficulties and opportunities she came across during fieldwork, especially how she adapted her ethnographic approach because accessibility to women proved to be a major challenge. In the conclusion, Ammann argues that flexibility and adaptation are key for ethnographic research. She pleads for more contributions in which not only female but also male researchers reflect on how their gendered presence influences their research settings