429 research outputs found

    Wavelets and Wavelet Sets

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    Wavelets are functions that are useful for representing signals and approximating other functions. Wavelets sets are defined in terms of Fourier transforms of certain wavelet functions. In this paper, we provide an introduction to wavelets and wavelets sets, examine the preexisting literature on the subject, and investigate an algorithm for creating wavelet sets. This algorithm creates single wavelets, which can be used to create bases for L2(Rn) through dilation and translation. We investigate the convergence properties of the algorithm, and implement the algorithm in Matlab

    Graham Gussin: FORSAKENFOCUSVERTIGOPREDICTION

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    Solo exhibition and publication, curated and edited by AR. ISBN 978-1-909693-07-

    Trehalase: A New Pollen Enzyme

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    Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factor

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    We have sequenced a cloned segment of E. coli chromosomal DNA that includes the heat shock regulatory gene htpR. This segment contains an 852 nucleotide open reading frame bounded by transcriptional and translational signals. Both in vivo and in vitro the cloned segment produces a single protein that migrates in gels with the cellular protein (F33.4) implicated as the htpR product. Properties of a cloned fragment of the coding sequence truncated at the promoter-distal end are consistent with this assignment. The htpR gene product appears homologous to the sigma factor of RNA polymerase, and the two proteins are predicted to have similar secondary structure. In addition, two regions of the predicted htpR product resemble protein-DNA contact points conserved in known DNA-binding proteins.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/24738/1/0000160.pd

    Quantum Dots for Live Cell and In Vivo Imaging

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    In the past few decades, technology has made immeasurable strides to enable visualization, identification, and quantitation in biological systems. Many of these technological advancements are occurring on the nanometer scale, where multiple scientific disciplines are combining to create new materials with enhanced properties. The integration of inorganic synthetic methods with a size reduction to the nano-scale has lead to the creation of a new class of optical reporters, called quantum dots. These semiconductor quantum dot nanocrystals have emerged as an alternative to organic dyes and fluorescent proteins, and are brighter and more stable against photobleaching than standard fluorescent indicators. Quantum dots have tunable optical properties that have proved useful in a wide range of applications from multiplexed analysis such as DNA detection and cell sorting and tracking, to most recently demonstrating promise for in vivo imaging and diagnostics. This review provides an in-depth discussion of past, present, and future trends in quantum dot use with an emphasis on in vivo imaging and its related applications

    A CI-Independent Form of Replicative Inhibition: Turn Off of Early Replication of Bacteriophage Lambda

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    Several earlier studies have described an unusual exclusion phenotype exhibited by cells with plasmids carrying a portion of the replication region of phage lambda. Cells exhibiting this inhibition phenotype (IP) prevent the plating of homo-immune and hybrid hetero-immune lambdoid phages. We have attempted to define aspects of IP, and show that it is directed to repΞ» phages. IP was observed in cells with plasmids containing a Ξ» DNA fragment including oop, encoding a short OOP micro RNA, and part of the lambda origin of replication, oriΞ», defined by iteron sequences ITN1-4 and an adjacent high AT-rich sequence. Transcription of the intact oop sequence from its promoter, pO is required for IP, as are iterons ITN3–4, but not the high AT-rich portion of oriΞ». The results suggest that IP silencing is directed to theta mode replication initiation from an infecting repΞ» genome, or an induced repΞ» prophage. Phage mutations suppressing IP, i.e., Sip, map within, or adjacent to cro or in O, or both. Our results for plasmid based IP suggest the hypothesis that there is a natural mechanism for silencing early theta-mode replication initiation, i.e. the buildup of Ξ» genomes with oop+ oriΞ»+ sequence

    Low male voice repertoire in contemporary musical theatre: a studio and performance guide of selected songs 1996-2020

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    Thesis (DM) – Indiana University, Music, 2020Discussion of trends in low voice classification, voice science, current market and schooling practices prior to selection guidelines and in-depth analysis of songs from the recent musical theatre canon suitable for use in studio training or performance. Appendices showcase additional suggested repertoire, performer history, and where to find published versions of sheet music

    The Boy on the Beach : building community through play

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