568 research outputs found

    Learning with Heterogeneous Expectations in an Evolutionary World

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    Validation of the scale of positive and negative experience in a South African student sample

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    Abstract: This study investigated the validity of the Scale of Positive and Negative Experience (SPANE) in a South Africa context using a sample of 992 university students. Item fit and unidimensionality of the Positive and Negative Experience subscales were examined using a process of Rasch analysis. Reliability of the subscales was evaluated and correlations were between the SPANE, the Mental Health Continuum – Short Form (MHC-SF) and the Satisfaction with Life Scale (SWLS), were examined. In general, the SPANE exhibited good Rasch fit and reliability. However, there were two items, one from each the Positive and Negative Experience subscales respectively that exhibited some problems with Rasch fit. While the substantive effect of these problematic items on reliability was marginal, their identification as problematic corroborated analysis in another study, enjoining, if not their removal, certainly a direction for future research. Overall, the findings serve to both support the notion that in its present form the SPANE is a psychometrically sound instrument to measure positive and negative experience as a facet of well-being among South African university students, and to indicate directions for further research on the scale

    Purification and analytical characterization of an anti- CD4 monoclonal antibody for human therapy

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    A purification process for the monclonal anti-CD4 antibody MAX.16H5 was developed on an analytical scale using (NH&SO, precipitation, anion-exchange chromatography on MonoQ or Q-Sepharose, hydrophobic interaction chromatography on phenyl- Sepharose and gel filtration chromatography on Superdex 200. The purification schedule was scaled up and gram amounts of MAX.16H5 were produced on corresponding BioPilot columns. Studies of the identity, purity and possible contamination by a broad range of methods showed that the product was highly purified and free from contaminants such as mouse DNA, viruses, pyrogens and irritants. Overall, the analytical data confirm that the monoclonal antibody MAX.16H5 prepared by this protocol is suitable for human therapy

    Application of two phosphorus models with different complexities in a mesoscale river catchment

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    The water balance and phosphorus inputs of surface waters of the Weiße Elster catchment, Germany, have been quantified using the models GROWA/MEPhos and SWAT. A comparison of the model results shows small differences in the mean long-term total runoff for the entire study area. All relevant pathways of phosphorus transport were considered in MEPhos with phosphorus inputs resulting to about 65% from point sources. SWAT focuses on agricultural areas and estimates a phosphorus input of about 60% through erosion. The mean annual phosphorus input from erosion calculated with SWAT is six times higher than the estimation with MEPhos due to the differing model concepts. This shows the uncertainty contributed by the modelling description of phosphorus pathways

    The role of flood wave superposition in the severity of large floods

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    Abstract. The severity of floods is shaped not only by event- and catchment-specific characteristics but also depends on the river network configuration. At the confluence of relevant tributaries with the main river, flood event characteristics may change depending on the magnitude and temporal match of flood waves. This superposition of flood waves may potentially increase the flood severity downstream in the main river. However, this aspect has not been analysed for a large set of river confluences to date. To fill this gap, the role of flood wave superposition in the flood severity at downstream gauges is investigated in four large river basins in Germany and Austria (the Elbe, the Danube, the Rhine and the Weser). A novel methodological approach to analyse flood wave superposition is presented and applied to mean daily discharge data from 37 triple points. A triple point consists of three gauges: one in the tributary as well as one upstream and downstream of the confluence with the main river respectively. At the triple points, differences and similarities in flood wave characteristics between the main river and the tributary are analysed in terms of the temporal match and the magnitudes of flood peaks. At many of the confluences analysed, the tributary peaks consistently arrive earlier than the main river peaks, although high variability in the time lag is generally detected. No large differences in temporal matching are detected for floods of different magnitudes. In the majority of cases, the largest floods at the downstream gauge do not occur due to perfect temporal match between the tributary and the main river. In terms of spatial variability, the impact of flood wave superposition is site-specific. Characteristic patterns of flood wave superposition are detected for flood peaks in the Danube River, where peak discharges largely increase due to inflow from alpine tributaries. Overall, we conclude that the superposition of flood waves is not the driving factor behind flood peak severity at the major confluences in Germany; however, a few confluences show the potential for strong flood magnifications if a temporal shift in flood waves was to occur
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