Designation of a Neotype for \u3ci\u3eOeneis melissa\u3c/i\u3e (\u3ci\u3eFabricius\u3c/i\u3e, 1775) and a Lectotype for \u3ci\u3eOeneis polixenes\u3c/i\u3e (\u3ci\u3eFabricius\u3c/i\u3e, 1775) (Lepidoptera: Nymphalidae: Satyrinae)

The Oeneis melissa (Fabricius, 1775) “neotype” designations of Lukhtanov and Eitschberger (2000; 2001) do not meet several critical requirements of the ICZN and are therefore invalid. In addition, the locality data is too vague to be taxonomically useful, their “neotype” specimen is a specimen of the taxon generally known as O. melissa semplei, and acceptance of their specimen as neotype would change the meaning of the taxon Oeneis melissa. Hence, I reject their neotype designation. A neotype Oeneis melissa (Fabricius, 1775) is designated from Nain, Labrador with careful consideration of the Articles of the International Code of Zoological Nomenclature. The ‘barcode’ mtDNA (CO1 gene) structure for the neotype is provided. The original number of type specimens of Oeneis polixenes (Fabricius, 1775) is unknown and a holotype was not designated explicitly or through implication; therefore, the type specimens are syntypes. There is one specimen that has been identified as a syntype of O. polixenes in the Natural History Museum in London (formerly the British Museum of Natural History), and no other extant specimens are known. I have expressly and deliberately designated the specimen in the collection of the Natural History Museum in London, as the lectotype of Oeneis polixenes (Fabricius, 1775) (ICZN Article 74.7.3) to fix a specific phenotype for the name

The Nomenclatural Status of Ten Names in the Genus \u3ci\u3ePieris\u3c/i\u3e (Lepidoptera: Pieridae)

The nomenclatural status of ten names in the genus Pieris are reviewed. The dates and locations of publication of pseudobryoniae Verity, 1908 and adalwinda Fruhstorfer, 1909 are corrected; they are infrasubspecific unavailable names. The names pseudobryoniae Barnes and McDunnough, 1916 and arctica Barnes and McDunnough, 1916 are newly identified as available species-group names with at least four and six syntypes respectively. A lectotype of pseudobryoniae Barnes and McDunnough is selected, with the type locality being Nulato, Alaska; browni Eitschberger, 1983 is a subjective synonym. A lectotype of arctica Barnes and McDunnough is designated, with the type locality being northern Norway. The name arctica Verity, 1911 is an infrasubspecific unavailable name. The date and location of publication of pseudonapi Verity, 1909 is corrected; it is an available name with pseudonapi Barnes & McDunnough, 1916 a primary homonym. The name macdunnoughii Remington, 1954 is the correct and original spelling; Miller and Brown (1981) provide the incorrect subsequent spelling mcdunnoughi. The name passosi Warren, 1968 is an available species-group name. A lectotype for passosi is designated; the name meckyae Eitschberger, 1983 is a subjective synonym. A lectotype is designated for pallidissima Barnes and McDunnough, 1916. The name angelika Eitschberger, 1981 is a nomen nudum, but angelika Eitschberger, 1983 is an available name. We suggest placing two taxa as subspecies of P. angelika, resulting in the combinations P. angelika sheljuzhkoi Eitschberger, 1983 and P. angelika schintlmeisteri Eitschberger, 1983. The publication date of Ulf Eitschberger’s Systematische untersuchungen am Pieris napi-bryoniae-komplex (s. l.) (Lepidoptera, Pieridae) is determined to be sometime in December 1983, nominally placed as December 31, 1983

Name-Bearing Types and Taxonomic Synopsis of Three Lycaenid Butterfly Taxa from Western Canada (Lepidoptera: Lycaenidae)

We clarify which of two designated lectotypes of Chrysophanus florus Edwards, 1884 is valid. We also show that the putative holotype of Plebeius saepiolus insulanus Blackmore, 1920 is actually a lectotype. A valid neotype designation for Lycaena saepiolus amica Edwards, 1863 is provided. Publication dates are corrected and we also briefly review the various taxonomic interpretations and distributions that appear in the literature with respect to these taxa

Standardization and methodological considerations for the isometric mid-thigh pull

The isometric mid-thigh pull (IMTP) is commonly used to assess an athlete’s force generation ability. This test is highly reliable and is simple and relatively quick to perform. The data that can be determined from the force-time curves generated by the test have been shown to be closely related to performance capacities in a variety of dynamic athletic tasks. However, within the scientific literature there are inconsistencies in the data collection procedures and methods used for data analysis that may impact the resultant output and the ability to compare and generalize results. Therefore, the primary aim of this review is to identify the differences in IMTP testing procedures and data analysis techniques, while identifying the potential impact this may have on the data collected. The secondary aim is to provide recommendations for the standardization of testing procedures to ensure that future IMTP data is of maximal benefit to practitioners and researchers

Integrin α2β1 Expression Regulates Matrix Metalloproteinase-1-Dependent Bronchial Epithelial Repair in Pulmonary Tuberculosis.

Pulmonary tuberculosis (TB) is caused by inhalation of Mycobacterium tuberculosis, which damages the bronchial epithelial barrier to establish local infection. Matrix metalloproteinase-1 plays a crucial role in the immunopathology of TB, causing breakdown of type I collagen and cavitation, but this collagenase is also potentially involved in bronchial epithelial repair. We hypothesized that the extracellular matrix (ECM) modulates M. tuberculosis-driven matrix metalloproteinase-1 expression by human bronchial epithelial cells (HBECs), regulating respiratory epithelial cell migration and repair. Medium from monocytes stimulated with M. tuberculosis induced collagenase activity in bronchial epithelial cells, which was reduced by ~87% when cells were cultured on a type I collagen matrix. Matrix metalloproteinase-1 had a focal localization, which is consistent with cell migration, and overall secretion decreased by 32% on type I collagen. There were no associated changes in the specific tissue inhibitors of metalloproteinases. Decreased matrix metalloproteinase-1 secretion was due to ligand-binding to the α2β1 integrin and was dependent on the actin cytoskeleton. In lung biopsies, samples from patients with pulmonary TB, integrin α2β1 is highly expressed on the bronchial epithelium. Areas of lung with disrupted collagen matrix showed an increase in matrix metalloproteinases-1 expression compared with areas where collagen was comparable to control lung. Type I collagen matrix increased respiratory epithelial cell migration in a wound-healing assay, and this too was matrix metalloproteinase-dependent, since it was blocked by the matrix metalloproteinase inhibitor GM6001. In summary, we report a novel mechanism by which α2β1-mediated signals from the ECM modulate matrix metalloproteinase-1 secretion by HBECs, regulating their migration and epithelial repair in TB

An engaged approach to exploring issues around poverty and mental health: A reflective evaluation of the research process from researchers and community partners involved in the DeStress study

Background Involving patients, service users, carers and members of the public in research has been part of health policy and practice in the UK for the last 15 years. However, low‐income communities tend to remain marginalized from the co‐design and delivery of mental health research, perpetuating the potential for health inequalities. Greater understanding is therefore needed on how to meaningfully engage low‐income communities in mental health research. Objectives To explore and articulate whether and how an engaged research approach facilitated knowledge coproduction relating to poverty and mental distress. Setting A reflective evaluation of community and researcher engagement in the DeStress study that took place in two low‐income areas of South‐west England. Design Reflective evaluation by the authors through on‐going feedback, a focus group and first‐person writing and discussion on experiences of working with the DeStress project, and how knowledge coproduction was influenced by an engaged research approach. Results An engaged research approach influenced the process and delivery of the DeStress project, creating a space where community partners felt empowered to coproduce knowledge relating to poverty‐related mental distress, treatment and the training of health professionals that would otherwise have been missed. We examine motivations for involvement, factors sustaining engagement, how coproduction influenced research analysis, findings and dissemination of outputs, and what involvement meant for different stakeholders. Conclusion Engaged research supported the coproduction of knowledge in mental health research with low‐income communities which led to multiple impacts

Phosphorus bioavailability in soil profiles of a long-term fertilizer experiment: The evaluation of their bioaccessibility

Global agricultural productivity depends on the use of finite phosphorus (P) resources of which not only the topsoil, but also subsoil, can hold immense reserves. To assess potential soil contribution to plant nutrition, we compared the P status of Stagnic Cambisol profiles in experimental plots that received different P fertilizer applications (control, triple superphosphate (TSP), compost, compost+TSP) for 16 years. Sequential fractionation was combined with P K-edge X-ray absorption near edge structure (XANES) spectroscopy to identify the chemical P speciation. Fertilized topsoils (21 to 69 kg P ha-1 a-1) showed P reserves larger by a factor of 1.2 to 1.4, and subsoil P reserves larger by a factor of 1.3 to 1.5 than those of the control. P-XANES revealed the predominance of inorganic P species such as moderately labile Fe- (46 to 92%), Al- (0 to 40%), and Ca- (0 to 15%) P compounds besides organic P (0 to 13%) in all treatments. The fertilizer application slightly altered P speciation throughout the profiles, but the type of fertilizer had no significant effect on it. Optimal plant growth requirements are restricted by the exchangeable P from the solid phase within the soil solution. Therefore, ongoing research focuses on the accessibility of P from P loaded amorphous Fe- and Al-hydroxides, previously identified as the predominant abiotic P forms. To assess their P desorption potential, P-33 rhizotron experiments combined with P-33 isotopic exchange kinetics (IEK) are underway. Preliminary results indicated that besides differences in P binding capacity of soil hydroxides, physical soil parameters, such as the matric potential, strongly control soil P availability, thus plant P acquisition rates can vary among different soil types. Our results gained new detailed information about P bioavailability under agricultural practice. The investigations towards P bioaccessibility may contribute to improved interpretation of soil P tests and reduced fertilizer recommendations

Rapid and complete paraffin removal from human tissue sections delivers enhanced Raman spectroscopic and histopathological analysis

Incomplete removal of paraffin and organic contaminants from tissues processed for diagnostic histology has been a profound barrier to the introduction of Raman spectroscopic techniques into clinical practice. We report a route to rapid and complete paraffin removal from a range of formalin-fixed paraffin embedded tissues using super mirror stainless steel slides. The method is equally effective on a range of human and animal tissues, performs equally well with archived and new samples and is compatible with standard pathology lab procedures. We describe a general enhancement of the Raman scatter and enhanced staining with antibodies used in immunohistochemistry for clinical diagnosis. We conclude that these novel slide substrates have the power to improve diagnosis through anatomical pathology by facilitating the simultaneous combination of improved, more sensitive immunohistochemical staining and simplified, more reliable Raman spectroscopic imaging, analysis and signal processing