Coccolith Morphology and Paleoclimatology - 2. Cell Ultrastructure and Formation of Coccoliths in Cyclococcolithina leptopora (Murray and Blackman) Wilcoxon and Gephyrocapsa oceanica Kamptner.

Current interest in utilization of coccoliths for paleoclimate reconstruction necessitates background information on environmental limits for growth and coccolith production as well as examination of cell ultrastructure in specimens collected in the field and in cultured representatives. Successful isolation of the two geologically important species Gephyrocapsa oceanica (strain A674) and Cyclococcolithina leptopora (strain A650) allows investigation of ultrastructure in cultured forms. Fine structure of cells and coccoliths was observed in the SEM using critical point dried preparations and ultrastructure was examined with the transmission electron microscope. Coccoliths are formed intracellularly and appear to form within Golgi-derived vesicles located near the nuclear membrane. Formation and development of coccoliths in the two species resemble these processes in Emiliania huxleyi but differ from those of Cricosphaera carterae, notably in the absence of coccolithosomes and scales and in the fact that coccoliths are produced intracellularly one at a time

The accumulation of barium by marine phytoplankton grown in culture

Marine phytoplankton have been implicated as potentially important vectors for the vertical transport of barium in the oceans. To better assess the extent to which phytoplankton can influence the geochemical cycling of barium, its bioconcentration was studied in 21 clones of 19 species of marine phytoplankters belonging to 9 algal classes. Barium levels in the ash ranged from less than 2 μg g–1 for the coccolithophore Emiliania huxleyi and the red alga Porphyridium cruenturn to 589 μg g–1 for the flagellate Tetraselmis levis. Concentrations ≥4000 μg g–1, previously reported for certain samples of diatom ash were not encountered in this study. Concentration factors on a volume basis (VCF) ranged from 0 to 3.2 × 104; the geometric mean VCF for all species was 225. Diatoms and coccolithophores generally had lower VCFs (geometric means of 90 and 12, respectively) than did other species; dinoflagellates had a geometric mean VCF of 490. Experiments with the diatom Thalassiosira pseudonana indicated that Ba cell–1 increased linearly with ambient Ba concentration. Experiments to localize the site of Ba deposition in diatom cells indicated that most of the Ba was associated with the frustules rather than with the organic fraction. Dinoflagellates and several other algae not only concentrated Ba to relatively high levels, but also accumulated Si when grown in Si-enriched medium, although they grew at least as well without added Si as with it. Ba and Si accumulation were generally negatively correlated

Congenital dislocation of the hip: Optimal screening strategies in 2014

AbstractA prospective multi-centre nationwide study of patients with congenital dislocation of the hip (CDH) diagnosed after 3 months of age was conducted with support from the French Society for Paediatric Orthopaedics (Société Française d’Orthopédie Pédiatrique [SoFOP]), French Organisation for Outpatient Paediatrics (Association Française de Pédiatrie Ambulatoire [AFPA]), and French-Speaking Society for Paediatric and Pre-Natal Imaging (Société Francophone d’Imagerie Pédiatrique et Prénatale [SFIPP]). The results showed inadequacies in clinical screening for CDH that were patent when assessed quantitatively and probably also present qualitatively. These findings indicate a need for a communication and educational campaign aimed at highlighting good clinical practice guidelines in the field of CDH screening. The usefulness of routine ultrasound screening has not been established. The findings from this study have been used by the authors and French National Health Authority (Haute Autorité de Santé [HAS]) to develop recommendations about CDH screening. There is an urgent need for a prospective randomised multi-centre nationwide study, which should involve primary-care physicians

Optical performance of the JWST MIRI flight model: characterization of the point spread function at high-resolution

The Mid Infra Red Instrument (MIRI) is one of the four instruments onboard the James Webb Space Telescope (JWST), providing imaging, coronagraphy and spectroscopy over the 5-28 microns band. To verify the optical performance of the instrument, extensive tests were performed at CEA on the flight model (FM) of the Mid-InfraRed IMager (MIRIM) at cryogenic temperatures and in the infrared. This paper reports on the point spread function (PSF) measurements at 5.6 microns, the shortest operating wavelength for imaging. At 5.6 microns the PSF is not Nyquist-sampled, so we use am original technique that combines a microscanning measurement strategy with a deconvolution algorithm to obtain an over-resolved MIRIM PSF. The microscanning consists in a sub-pixel scan of a point source on the focal plane. A data inversion method is used to reconstruct PSF images that are over-resolved by a factor of 7 compared to the native resolution of MIRI. We show that the FWHM of the high-resolution PSFs were 5-10% wider than that obtained with Zemax simulations. The main cause was identified as an out-of-specification tilt of the M4 mirror. After correction, two additional test campaigns were carried out, and we show that the shape of the PSF is conform to expectations. The FWHM of the PSFs are 0.18-0.20 arcsec, in agreement with simulations. 56.1-59.2% of the total encircled energy (normalized to a 5 arcsec radius) is contained within the first dark Airy ring, over the whole field of view. At longer wavelengths (7.7-25.5 microns), this percentage is 57-68%. MIRIM is thus compliant with the optical quality requirements. This characterization of the MIRIM PSF, as well as the deconvolution method presented here, are of particular importance, not only for the verification of the optical quality and the MIRI calibration, but also for scientific applications.Comment: 13 pages, submitted to SPIE Proceedings vol. 7731, Space Telescopes and Instrumentation 2010: Optical, Infrared, and Millimeter Wav

Contemporary integrative taxonomy for sexually deprived protists: A case study of Trachelomonas (Euglenaceae) from western Ukraine

As many other protist groups, euglenophytes are prone to false identification based solely on morphology because of a limited amount of morphological features and cryptic speciation. One of the supposedly completely asexual groups within the freshwater phototrophic representatives of euglenophytes is Trachelomonas , capable of forming an inorganic shell around its cell (i.e., the lorica). The International Code of Nomenclature for algae, fungi, and plants regulates the taxonomy not only of flowering plants, but explicitly also of phototrophic protists, and provides powerful tools to resolve various taxonomic challenges. To exemplify some of the problems and potential solutions, a number of Trachelomonas strains were collected from the muddy, lake‐rich region of Dobrostany and cultivated under stable laboratory conditions. Being a type locality of 58 unclarified Trachelomonas names, this region in western Ukraine is of great taxonomic importance. Based on light and electron microscopy, and on RAxML and MrBayes phylogenetics using multiple loci and a representative taxon sample, a detailed description of investigated strains and their systematic placement is provided. Morphologically, the strains differed slightly but consistently in minute characters such as size, lorica shape and ornamentation. The presently most comprehensive molecular tree of the Euglenaceae indicated to the existence of at least five different species present in the newly investigated samples, although they were collected from localities in very close vicinity to each other and at the same date. Based on morphological comparisons with type illustrations of species validly described 100 or more years ago, biological material was used to epitypify three names of Trachelomonas , eternally linking morphology with reliable genetic information. This taxonomic application is one of the powerful methods to clarify ambiguous scientific names, which has particular importance in character‐poor protists such as the euglenophytes

Empirical bayes analysis of sequencing-based transcriptional profiling without replicates

Background: Recent technological advancements have made high throughput sequencing an increasingly popular approach for transcriptome analysis. Advantages of sequencing-based transcriptional profiling over microarrays have been reported, including lower technical variability. However, advances in technology do not remove biological variation between replicates and this variation is often neglected in many analyses. Results: We propose an empirical Bayes method, titled Analysis of Sequence Counts (ASC), to detect differential expression based on sequencing technology. ASC borrows information across sequences to establish prior distribution of sample variation, so that biological variation can be accounted for even when replicates are not available. Compared to current approaches that simply tests for equality of proportions in two samples, ASC is less biased towards highly expressed sequences and can identify more genes with a greater log fold change at lower overall abundance. Conclusions: ASC unifies the biological and statistical significance of differential expression by estimating the posterior mean of log fold change and estimating false discovery rates based on the posterior mean. The implementation in R is available at http://www.stat.brown.edu/Zwu/research.aspx

H_2 emission arises outside photodissociation regions in ultra-luminous infrared galaxies

Ultra-luminous infrared galaxies are among the most luminous objects in the local universe and are thought to be powered by intense star formation. It has been shown that in these objects the rotational spectral lines of molecular hydrogen observed at mid-infrared wavelengths are not affected by dust obscuration, leaving unresolved the source of excitation of this emission. Here I report an analysis of archival Spitzer Space Telescope data on ultra-luminous infrared galaxies and demonstrate that star formation regions are buried inside optically thick clouds of gas and dust, so that dust obscuration affects star-formation indicators but not molecular hydrogen. I thereby establish that the emission of H_2 is not co-spatial with the buried starburst activity and originates outside the obscured regions. This is rather surprising in light of the standard view that H_2 emission is directly associated with star-formation activity. Instead, I propose that H_2 emission in these objects traces shocks in the surrounding material, which are in turn excited by interactions with nearby galaxies, and that powerful large-scale shocks cooling by means of H_2 emission may be much more common than previously thought. In the early universe, a boost in H_2 emission by this process may speed up the cooling of matter as it collapsed to form the first stars and galaxies and would make these first structures more readily observable.Comment: Main text and supplemental information, 21 pages including 6 figures, 2 table

Nuclear high-ionisation outflow in the Compton-thick AGN NGC6552 as seen by the JWST mid-infrared instrument

During the commissioning of the James Webb Space Telescope (JWST), the mid-infrared instrument (MIRI) observed NGC6552 with the MIRI Imager and the medium-resolution spectrograph (MRS). NGC6552 is an active galactic nucleus (AGN) at redshift 0.0266 classified as a Seyfert 2 nucleus in the optical, and Compton-thick AGN in X-rays. This work exemplifies and demonstrates the MRS capabilities to study the mid-infrared (mid-IR) spectra and characterize the physical conditions and kinematics of the ionized and molecular gas in the nuclear regions of nearby galaxies. We obtained the nuclear, circumnuclear, and central mid-IR spectra of NGC6552. They provide the first clear observational evidence for a nuclear outflow in NGC6552. The outflow contributes to 67$\pm$7% of the total line flux independent of the ionization potential (27 to 187 eV) and critical densities (10$^4$ to 4$\times$10$^{6}$ cm$^{-3}$), showing an average blue-shifted peak velocity of -127$\pm$45 kms$^{-1}$ and an outflow maximal velocity of 698$\pm$80 kms$^{-1}$. Since the mid-IR photons penetrate dusty regions as efficiently as X-ray keV photons, we interpret these results as the evidence for a highly ionized, non-stratified, AGN-powered, and fast outflowing gas in a low density environment (few 10$^{3}$ cm$^{-3}$) located very close (<0.2kpc) to the Compton-thick AGN. Nine pure rotational molecular Hydrogen lines are detected and spectrally resolved, and exhibit symmetric Gaussian profiles, consistent with the galactic rotation, and with no evidence of outflowing H$_{2}$ material. We detect a warm H$_{2}$ mass of $1.9\pm1.1\times10^7 M_{\odot}$ in the central region (1.8 kpc in diameter) of the galaxy, with almost 30% of that mass in the circum-nuclear region. Line ratios confirm that NGC6552 has a Seyfert nucleus with a black hole mass estimated in the range of 0.6 to 6 million solar masses.Comment: 13 pages, 5 figures, 5 tables, accepted in A&

The Mid-Infrared Instrument for the James Webb Space Telescope, III: MIRIM, The MIRI Imager

In this article, we describe the MIRI Imager module (MIRIM), which provides broad-band imaging in the 5 - 27 microns wavelength range for the James Webb Space Telescope. The imager has a 0"11 pixel scale and a total unobstructed view of 74"x113". The remainder of its nominal 113"x113" field is occupied by the coronagraphs and the low resolution spectrometer. We present the instrument optical and mechanical design. We show that the test data, as measured during the test campaigns undertaken at CEA-Saclay, at the Rutherford Appleton Laboratory, and at the NASA Goddard Space Flight Center, indicate that the instrument complies with its design requirements and goals. We also discuss the operational requirements (multiple dithers and exposures) needed for optimal scientific utilization of the MIRIM.Comment: 29 pages, 9 figure