Involvement of Thyroid Hormones in the Expression of MHC class I Antigens During Ontogeny in Xenopus

The major histocompatibility complex (MHC) is a cluster of genes encoding products central to all major functions of the vertebrate immune system. Evidence for an MHC can be found in all vertebrate groups that have been examined except the jawless fishes. Expression of MHC class I and class II antigens early in ontogeny is critically important for development of T lymphocytes capable of discriminating self from nonself. Because of this essential role in T-cell development, the ontogeny of MHC expression in the South African clawed frog, Xenopus laevis, was studied. Previous studies of MHC class I expression in Xenopus laevis suggested that class I antigens are virtually absent from tadpole tissues until climax of metamorphosis. We therefore examined the possible role of thyroid hormones (TH) in the induction of class I. By flow cytometry, a small amount of class I expression was detectable on splenocytes and erythrocytes in untreated frogs at prometamorphic stages 55-58, and the amount increased significantly at the conclusion of metamorphic climax. Thus, metamorphosis is associated with increased intensity of class I expression. Neither inhibition nor acceleration of metamorphosis altered the timing of onset of class I expression. However, inhibition of metamorphosis prevented the increase in class I expression characteristic of adult cell populations. Because expression was not accelerated in TH-treated frogs or delayed in metamorphosis-inhibited frogs, it is unlikely that TH are the direct developmental cues that induce expression, although they seem to be required for the upregulation of class I expression occurring at metamorphosis. Differences in the pattern of expression in different subpopulations of cells suggest a complex pattern of regulation of expression of class I antigens during ontogeny

Involvement of Thyroid Hormones in the Expression of MHC class I Antigens During Ontogeny in Xenopus

The major histocompatibility complex (MHC) is a cluster of genes encoding products central to all major functions of the vertebrate immune system. Evidence for an MHC can be found in all vertebrate groups that have been examined except the jawless fishes. Expression of MHC class I and class II antigens early in ontogeny is critically important for development of T lymphocytes capable of discriminating self from nonself. Because of this essential role in T-cell development, the ontogeny of MHC expression in the South African clawed frog, Xenopus laevis, was studied. Previous studies of MHC class I expression in Xenopus laevis suggested that class I antigens are virtually absent from tadpole tissues until climax of metamorphosis. We therefore examined the possible role of thyroid hormones (TH) in the induction of class I. By flow cytometry, a small amount of class I expression was detectable on splenocytes and erythrocytes in untreated frogs at prometamorphic stages 55-58, and the amount increased significantly at the conclusion of metamorphic climax. Thus, metamorphosis is associated with increased intensity of class I expression. Neither inhibition nor acceleration of metamorphosis altered the timing of onset of class I expression. However, inhibition of metamorphosis prevented the increase in class I expression characteristic of adult cell populations. Because expression was not accelerated in TH-treated frogs or delayed in metamorphosis-inhibited frogs, it is unlikely that TH are the direct developmental cues that induce expression, although they seem to be required for the upregulation of class I expression occurring at metamorphosis. Differences in the pattern of expression in different subpopulations of cells suggest a complex pattern of regulation of expression of class I antigens during ontogeny

The Impact of Menthol Cigarettes on Smoking Initiation among Non-Smoking Young Females in Japan

Japan presents an excellent case-study of a nation with low female smoking rates and a negligible menthol market which changed after the cigarette market was opened to foreign competition. Internal tobacco industry documents demonstrate the intent of tobacco manufacturers to increase initiation among young females through development and marketing of menthol brands. Japanese menthol market share rose rapidly from less than 1% in 1980 to 20% in 2008. Menthol brand use was dominated by younger and female smokers, in contrast with non-menthol brands which were used primarily by male smokers. Nationally representative surveys confirm industry surveys of brand use and provide further evidence of the end results of the tobacco industry’s actions—increased female smoking in Japan. These findings suggest that female populations may be encouraged to initiate into smoking, particularly in developing nations or where female smoking rates remain low, if the tobacco industry can successfully tailor brands to them. The Japanese experience provides a warning to public health officials who wish to prevent smoking initiation among young females

Disease prevention strategies for QX disease (Marteilia sydneyi) of Sydney rock oysters (Saccostrea glomerata)

The Sydney rock oyster (Saccostrea glomerata) forms the basis of an important aquaculture industry on the east coast of Australia. During the 1970s, production of S. glomerata began to decline, in part as a result of mortalities arising from Queensland unknown (QX) disease. Histological studies implicated the paramyxean parasite Marteilia sydneyi in the disease outbreaks. Disease zoning was implemented to prevent the spread of M. sydneyi-infected oysters. This control measure hindered rock oyster farming, which historically has relied on transferring wild-caught spat between estuaries for on-growing to market size and has not prevented the subsequent occurrence of QX disease in the Georges and Hawkesbury rivers in central New South Wales. Management of QX disease has been hampered by the complicated life cycle of M. sydneyi, with outbreaks of QX disease likely to be regulated by a combination of the abundance of intermediate host of M. sydneyi, environmental stressors, and the immunocompetence of S. glomerata. The future of the Sydney rock oyster industry relies on understanding these factors and progressing the industry from relying on farming wild-caught seed to the successful commercialization of hatchery-produced QX-resistant S. glomerata

Probing the Balance of AGN and Star-Forming Activity in the Local Universe with ChaMP

The combination of the SDSS and the Chandra Multiwavelength Project (ChaMP) currently offers the largest and most homogeneously selected sample of nearby galaxies for investigating the relation between X-ray nuclear emission, nebular line-emission, black hole masses, and properties of the associated stellar populations. We present here novel constraints that both X-ray luminosity Lx and X-ray spectral energy distribution bring to the galaxy evolutionary sequence H II -> Seyfert/Transition Object -> LINER -> Passive suggested by optical data. In particular, we show that both Lx and Gamma, the slope of the power-law that best fits the 0.5 - 8 keV spectra, are consistent with a clear decline in the accretion power along the sequence, corresponding to a softening of their spectra. This implies that, at z ~ 0, or at low luminosity AGN levels, there is an anti-correlation between Gamma and L/Ledd, opposite to the trend exhibited by high z AGN (quasars). The turning point in the Gamma -L/Ledd LLAGN + quasars relation occurs near Gamma ~ 1.5 and L/Ledd ~ 0.01. Interestingly, this is identical to what stellar mass X-ray binaries exhibit, indicating that we have probably found the first empirical evidence for an intrinsic switch in the accretion mode, from advection-dominated flows to standard (disk/corona) accretion modes in supermassive black hole accretors, similar to what has been seen and proposed to happen in stellar mass black hole systems. The anti-correlation we find between Gamma and L/Ledd may instead indicate that stronger accretion correlates with greater absorption. Therefore the trend for softer spectra toward more luminous, high redshift, and strongly accreting AGN/quasars could simply be the result of strong selection biases reflected in the dearth of type 2 quasar detections.Comment: 23 pages, 8 figures, 1 long (3 page) table, to appear in Ap

Search for quarks in cosmic rays with the Leeds cloud chamber

The central regions of cosmic-ray air showers near sea level have been studied with the Leeds cloud chamber for the possible occurrence of low-ionizing tracks. The average energy of the primary particles was a few times 106 GeV. Our current results give an upper limit to the "flux" of quarks of 1.2 x 10-11 cm-2 sec-1 sr-1 at a 90% confidence level. A simple model is used to obtain an upper limit to the production cross section versus quark mass.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/21994/1/0000406.pd

A Multiwavelength Study of Binary Quasars and Their Environments

We present Chandra X-ray imaging and spectroscopy for 14 quasars in spatially resolved pairs, part of a complete sample of binary quasars with small transverse separations drawn from Sloan Digital Sky Survey (DR6) photometry. We find no significant difference in X-ray properties when compared with large control samples of isolated quasars. We present infrared photometry from our observations with SWIRC at the MMT, and from the WISE Preliminary Data Release, and fit simple spectral energy distributions to all 14 QSOs. We find preliminary evidence that substantial contributions from star formation are required, but possibly no more so than for isolated X-ray-detected QSOs. Sensitive searches of the X-ray images for extended emission, and the optical images for optical galaxy excess show that these binary QSOs are not preferentially found in rich cluster environments. While larger binary QSO samples with richer far-IR and sub-millimeter multiwavelength data might better reveal signatures of merging and triggering, optical color-selection of QSO pairs may be biased against such signatures. X-ray and/or variability selection of QSO pairs, while challenging, should be attempted. We present in our Appendix a primer on X-ray flux and luminosity calculations.Comment: 21 pages, accepted to ApJ 08/31/201