Survey of Dairy Management Practices on One Hundred Thirteen North Central and Northeastern United States Dairies

The objective was to conduct a broad survey of dairy management practices that have an effect on animal well-being. Dairies were visited during the fall and winter of 2005 and 2006 in Wisconsin, Minnesota, Indiana, Iowa, and New York. Data were collected on 113 dairies on colostrum feeding, dehorning, tail-docking, euthanasia methods, producer statements about welfare, use of specialized calf-raising farms (custom), level of satisfaction with calf-raising by producers, and cow behavior. Calves were raised by the owner on 50.4% of dairies; 30.1% were raised on custom farms during the milk-feeding period, 18.6% were custom raised after weaning, and 1% sold calves with the option to buy them back as first-lactation heifers. A total of 51.8% of producers were very satisfied with their current calf-raising methods. Three feedings of colostrum were fed to the calves on 23.9% of dairies, 2 feedings on 39.8% of farms, 1 feeding on 31.0% of farms, and colostrum replacement products were fed on 5.3% of farms. Many farms (61.9%) provided 3.8 L at first feeding. Calves were dehorned at different ages by various methods. By 8 wk, 34.5% of calves were dehorned. By 12 wk, 78.8% of calves were dehorned. The majority of calves were dehorned by hot iron (67.3%). The remainder were dehorned by gouging (8.8%), paste (9.7%), saw (3.5%), or unknown by calf owner (10.6%). Anesthetic use was reported by 12.4% of dairy owners and analgesia use by 1.8%. Tail-docking was observed on 82.3% of dairies. The most common reported docking time was pre- or postcalving (35.2%). The second most commonly reported time was d 1 (15.4%). Rubber band was the most common method (92.5%), followed by amputation (7.5%). Three dairies amputated precalving, 1 at 2 mo and 3 at d 1 or 2. Cow hygiene was the most common reason given to dock (73.5%), followed by parlor worker comfort (17.4%) and udder health (1.0%). Producers reported 2.0% of cows obviously lame. Gun was the preferred euthanasia method (85.7%), followed by i.v. euthanasia (8.0%), live pick-up (1.8%), and nondisclosure (3.5%). Most producers (77.9%) stated that cows were in an improved environment as compared with 20 yr ago, whereas 8.0% stated conditions were worse, and 14.2% were undecided. Dairies with higher percentages of cows that either approached or touched the observer had lower somatic cell counts. The survey results showed management practices that were important for animal welfare

Effect of stall base type on herd health, costs, and producer satisfaction

The objective of this field study was to compare effect of stall base on herd health, stall maintenance, bedding cost, and producer satisfaction. Ninety-one dairies visited during a 4-mo period starting October 14, 2005 included 33 rubber-filled mattress (RFM), 27 sand, and 31 waterbed (WB) dairies. In this study, percent culled was higher for RFM (P = 0.001) and sand (P = 0.06) than WB stall base dairies. Percent of cows in fourth lactation or greater was higher on WB than either RFM (P = 0.01) or sand (P = 0.02) dairies. There was no difference between base types for production or somatic cell count. Bedding cost per bed per week was WB ($0.73), RFM ($0.89), and sand ($0.97). Sand beds were bedded less frequently (P = 0.01). Comparisons between RFM and sand indicate higher satisfaction for RFM regarding manure management (P \u3c 0.0001) and higher satisfaction with sand for cow comfort (P \u3c 0.0001). Producers with WB were more satisfied with base life (P \u3c 0.000 1) and cow comfort (P \u3c 0.000 1) than those with RFM. Producers with WB were more satisfied with cow longevity (P \u3c 0.0001) as compared to RFM. Length of sand stall was correlated with longevity (0.56, P = 0.01) while percent of mature cows was greater on dairies that provided WBs (P = 0.02). This data indicates that WB may be a viable option for cows and producers, when good quality sand is unavailable or handling sand-laden manure is not feasible

Effect of stall base type on herd health, costs, and producer satisfaction

The objective of this field study was to compare effect of stall base on herd health, stall maintenance, bedding cost, and producer satisfaction. Ninety-one dairies visited during a 4-mo period starting October 14, 2005 included 33 rubber-filled mattress (RFM), 27 sand, and 31 waterbed (WB) dairies. In this study, percent culled was higher for RFM (P = 0.001) and sand (P = 0.06) than WB stall base dairies. Percent of cows in fourth lactation or greater was higher on WB than either RFM (P = 0.01) or sand (P = 0.02) dairies. There was no difference between base types for production or somatic cell count. Bedding cost per bed per week was WB ($0.73), RFM ($0.89), and sand ($0.97). Sand beds were bedded less frequently (P = 0.01). Comparisons between RFM and sand indicate higher satisfaction for RFM regarding manure management (P \u3c 0.0001) and higher satisfaction with sand for cow comfort (P \u3c 0.0001). Producers with WB were more satisfied with base life (P \u3c 0.000 1) and cow comfort (P \u3c 0.000 1) than those with RFM. Producers with WB were more satisfied with cow longevity (P \u3c 0.0001) as compared to RFM. Length of sand stall was correlated with longevity (0.56, P = 0.01) while percent of mature cows was greater on dairies that provided WBs (P = 0.02). This data indicates that WB may be a viable option for cows and producers, when good quality sand is unavailable or handling sand-laden manure is not feasible

Influence of Free-Stall Base on Tarsal Joint Lesions and Hygiene in Dairy Cows

The objective was to quantify the incidence of tarsal lesions and level of hygiene by stall bed type. Cows were scored on 100 dairies from Wisconsin, Minnesota, Indiana, Iowa, and New York in the fall and winter. Thirty-eight dairies used rubber-filled mattresses (RFM), 27 had sand beds, 29 had waterbeds, and 6 used compost packs (CPk). Stocking density, stall dimensions, bedding amount, bedding frequency, and type of bedding were recorded. One pen of early-lactation multiparous cows on each dairy was scored based on injury of the tarsal joints at the lateral and medial surfaces and tuber calcis at the dorsal, lateral, and medial surfaces. A tarsal score of 1 represented hair loss, 2 was moderate, and 3 indicated severe swelling. Differences between bed types in the percentages of cows with lesions were tested with one-way ANOVA by lesion severity and incidence, with farm as the experimental unit. Cows on sand beds or waterbeds had fewer lesion scores of 1, 2, and 3 than those on RFM. The percentages of score 1 were 54.6 ± 4.4 (RFM), 22.5 ± 4.7 (sand), and 29.8 ± 4.3 (waterbed), whereas the percentages of score 2 were 14.0 ± 1.4, 2.3 ± 1.5, and 5.0 ± 1.4, and of score 3 were 3.0 ± 0.4, 0.2 ± 0.4, and 0.4 ± 0.4. Cows on CPk had no lesions. Hygiene scores ranged from 1 to 5, with 1 being clean and 5 soiled. The percentages of hygiene score 1 were 0 (compost), 0.4 (RFM), 0.4 (sand), and 0.4 (waterbeds); those with score 2 were 79.0, 84.0 ± 0.01, 73.2 ± 0.01, and 80.4 ± 0.01; with score 3 were 20.3, 15.2 ± 0.01, 23.8 ± 0.01, and 18.6 ± 0.01; with score 4 were 0.8, 0.005 ± 0.001, 0.006 ± 0.001, and 0.025 ± 0.003; and with score 5 was 0 for all bed types. Cows on RFM and waterbeds had improved hygiene compared with cows on sand beds. There was no difference in somatic cell count (SCC) by bed type. The percentage of cows in fourth lactation or greater on waterbeds (19.8 ± 1.8) was greater than those on RFM (13.3 ± 1.6) or on sand (13.5 ± 1.8). The percentage culled was lower for cows on waterbeds than on RFM (22.8 ± 1.5 vs. 29.4 ± 1.4). Score 3 tarsal lesions were correlated (r = 0.60) with SCC. The length of the sand bed was correlated with a greater percentage of mature cows. The SCC was correlated with the percentage of cows reported lame on the day of the visit (r = 0.45) and with neck rail height (r = −0.26). On dairies with RFM, severe lesions (r = 0.60), death losses (r = 0.52), and percentage of the herd reported lame on the day of the visit (r = 0.52) were all correlated with the SCC. Dairies with higher percentages of lesions had higher SCC, death losses, lameness, and culling rates. Adding bedding several times per week may reduce the incidence of lesions

High Ice Water Content at Low Radar Reflectivity near Deep Convection: Part II. Evaluation of Microphysical Pathways in Updraft Parcel Simulations

The aeronautics industry has established that a threat to aircraft is posed by atmospheric conditions of substantial ice water content (IWC) where equivalent radar reflectivity (Ze) does not exceed 20-30 dBZ and supercooled water is not present; these conditions are encountered almost exclusively in the vicinity of deep convection. Part 1 (Fridlind et al., 2015) of this two-part study presents in situ measurements of such conditions sampled by Airbus in three tropical regions, commonly near 11 km and -43 C, and concludes that the measured ice particle size distributions are broadly consistent with past literature with profiling radar measurements of Z(sub e) and mean Doppler velocity obtained within monsoonal deep convection in one of the regions sampled. In all three regions, the Airbus measurements generally indicate variable IWC that often exceeds 2 gm (exp -3) with relatively uniform mass median area-equivalent diameter (MMD(sub eq) of 200-300 micrometers. Here we use a parcel model with size-resolved microphysics to investigate microphysical pathways that could lead to such conditions. Our simulations indicate that homogeneous freezing of water drops produces a much smaller ice MMD(sub eq) than observed, and occurs only in the absence of hydrometeor gravitational collection for the conditions considered. Development of a mass mode of ice aloft that overlaps with the measurements requires a substantial source of small ice particles at temperatures of about -10 C or warmer, which subsequently grow from water vapor. One conceivable source in our simulation framework is Hallett-Mossop ice production; another is abundant concentrations of heterogeneous ice freezing nuclei acting together with copious shattering of water drops upon freezing. Regardless of the production mechanism, the dominant mass modal diameter of vapor-grown ice is reduced as the ice-multiplication source strength increases and as competition for water vapor increases. Both mass and modal diameter are reduced by entrainment and by increasing aerosol concentrations. Weaker updrafts lead to greater mass and larger modal diameters of vapor-grown ice, the opposite of expectations regarding lofting of larger ice particles in stronger updrafts. While stronger updrafts do loft more dense ice particles produced primarily by raindrop freezing, we find that weaker updrafts allow the warm rain process to reduce competition for diffusional growth of the less dense ice expected to persist in convective outflow

Evaluation of radar reflectivity factor simulations of ice crystal populations from in situ observations for the retrieval of condensed water content in tropical mesoscale convective systems

This study presents the evaluation of a technique to estimate cloud condensed water content (CWC) in tropical convection from airborne cloud radar reflectivity factors at 94 GHz and in situ measurements of particle size distributions (PSDs) and aspect ratios of ice crystal populations. The approach is to calculate from each 5 s mean PSD and flight-level reflectivity the variability of all possible solutions of m(D) relationships fulfilling the condition that the simulated radar reflectivity factor (T-matrix method) matches the measured radar reflectivity factor. For the reflectivity simulations, ice crystals were approximated as oblate spheroids, without using a priori assumptions on the mass–size relationship of ice crystals. The CWC calculations demonstrate that individual CWC values are in the range ±32 % of the retrieved average CWC value over all CWC solutions for the chosen 5 s time intervals. In addition, during the airborne field campaign performed out of Darwin in 2014, as part of the international High Altitude Ice Crystals/High Ice Water Content (HAIC/HIWC) projects, CWCs were measured independently with the new IKP-2 (isokinetic evaporator probe) instrument along with simultaneous particle imagery and radar reflectivity. Retrieved CWCs from the T-matrix radar reflectivity simulations are on average 16 % higher than the direct CWCIKP measurements. The differences between the CWCIKP and averaged retrieved CWCs are found to be primarily a function of the total number concentration of ice crystals. Consequently, a correction term is applied (as a function of total number concentration) that significantly improves the retrieved CWC. After correction, the retrieved CWCs have a median relative error with respect to measured values of only −1 %. Uncertainties in the measurements of total concentration of hydrometeors are investigated in order to calculate their contribution to the relative error of calculated CWC with respect to measured CWCIKP. It is shown that an overestimation of the concentration by about +50 % increases the relative errors of retrieved CWCs by only +29 %, while possible shattering, which impacts only the concentration of small hydrometeors, increases the relative error by about +4 %. Moreover, all cloud events with encountered graupel particles were studied and compared to events without observed graupel particles. Overall, graupel particles seem to have the largest impact on high crystal number-concentration conditions and show relative errors in retrieved CWCs that are higher than for events without graupel particles

High Ice Water Content at Low Radar Reflectivity near Deep Convection

Occurrences of jet engine power loss and damage have been associated with flight through fully glaciated deep convection at -10 to -50 degrees Centigrade. Power loss events commonly occur during flight through radar reflectivity (Zeta (sub e)) less than 20-30 decibels relative to Zeta (dBZ - radar returns) and no more than moderate turbulence, often overlying moderate to heavy rain near the surface. During 2010-2012, Airbus carried out flight tests seeking to characterize the highest ice water content (IWC) in such low-radar-reflectivity regions of large, cold-topped storm systems in the vicinity of Cayenne, Darwin, and Santiago. Within the highest IWC regions encountered, at typical sampling elevations (circa 11 kilometers), the measured ice size distributions exhibit a notably narrow concentration of mass over area-equivalent diameters of 100-500 micrometers. Given substantial and poorly quantified measurement uncertainties, here we evaluate the consistency of the Airbus in situ measurements with ground-based profiling radar observations obtained under quasi-steady, heavy stratiform rain conditions in one of the Airbus-sampled locations. We find that profiler-observed radar reflectivities and mean Doppler velocities at Airbus sampling temperatures are generally consistent with those calculated from in situ size-distribution measurements. We also find that column simulations using the in situ size distributions as an upper boundary condition are generally consistent with observed profiles of radar reflectivity (Ze), mean Doppler velocity (MDV), and retrieved rain rate. The results of these consistency checks motivate an examination of the microphysical pathways that could be responsible for the observed size-distribution features in Ackerman et al. (2015)

CDK-dependent nuclear localization of B-Cyclin Clb1 promotes FEAR activation during meiosis I in budding yeast

Cyclin-dependent kinases (CDK) are master regulators of the cell cycle in eukaryotes. CDK activity is regulated by the presence, post-translational modification and spatial localization of its regulatory subunit cyclin. In budding yeast, the B-cyclin Clb1 is phosphorylated and localizes to the nucleus during meiosis I. However the functional significance of Clb1's phosphorylation and nuclear localization and their mutual dependency is unknown. In this paper, we demonstrate that meiosis-specific phosphorylation of Clb1 requires its import to the nucleus but not vice versa. While Clb1 phosphorylation is dependent on activity of both CDK and polo-like kinase Cdc5, its nuclear localization requires CDK but not Cdc5 activity. Furthermore we show that increased nuclear localization of Clb1 during meiosis enhances activation of FEAR (Cdc Fourteen Early Anaphase Release) pathway. We discuss the significance of our results in relation to regulation of exit from meiosis I

Ethics, space, and somatic sensibilities: comparing relationships between scientific researchers and their human and animal experimental subjects

Drawing on geographies of affect and nature-society relations, we propose a radical rethinking of how scientists, social scientists, and regulatory agencies conceptualise human and animal participants in scientif ic research. The scientific rationale for using animal bodies to simulate what could be done in human bodies emphasises shared somatic capacities that generate comparable responses to clinical interventions. At the same time, regulatory guidelines and care practices stress the differences between human and animal subjects. In this paper we consider the implications of this differentiation between human and animal bodies in ethical and welfare protocols and practices. We show how the bioethical debates around the use of human subjects tend to focus on issues of consent and language, while recent work in animal welfare reflects an increasing focus on the affectual dimensions of ethical practice. We argue that this attention to the more-than-representational dimensions of ethics and welfare might be equally important for human subjects. We assert that paying attention to these somatic sensibilities can offer insights into how experimental environments can both facilitate and restrict the development of more care-full and response-able relations between researchers and their experimental subjects. <br/