Fast protein modification in the nanomolar concentration range using an oxalyl amide as latent thioester

We show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on-demand for enabling ligation reactions down to peptide concentrations as low as a few hundreds nM at rates approaching 30 M-1 s-1

Fast protein modification in the nanomolar concentration range using an oxalyl amide as latent thioester

International audienceWe show that latent oxalyl thioester surrogates are a powerful means to modify peptides and proteins in highly dilute conditions in purified aqueous media or in mixtures as complex as cell lysates. Designed to be shelf-stable reagents, they can be activated on-demand for enabling ligation reactions down to peptide concentrations as low as a few hundreds nM at rates approaching 30 M-1 s-1

A cryogenic half-wave plate polarimeter using a superconducting magnetic bearing

We present the design and measured performance of the superconducting magnetic bearing (SMB) that was used successfully as the rotation mechanism in the half-wave plate polarimeter of the E and B Experiment (EBEX) during its North American test flight. EBEX is a NASA-supported balloon-borne experiment that is designed to measure the polarization of the cosmic microwave background. In this implementation the half-wave plate is mounted to the rotor of an SMB that is operating at the sink temperature of 4 K. We demonstrate robust, remote operation on a balloon-borne payload, with angular encoding accuracy of 0.01°. We find rotational speed variation to be 0.2% RMS. We measure vibrational modes and find them to be consistent with a simple SMB model. We search for but do not find magnetic field interference in the detectors and readout. We set an upper limit of 3% of the receiver noise level after 5 minutes of integration on such interference. At 2 Hz rotation we measure a power dissipation of 56 mW. If this power dissipation is reduced, such an SMB implementation is a candidate for low-noise space applications because of the absence of stick-slip friction and low wear

Concept design of the LiteBIRD satellite for CMB B-mode polarization

LiteBIRD is a candidate for JAXA's strategic large mission to observe the cosmic microwave background (CMB) polarization over the full sky at large angular scales. It is planned to be launched in the 2020s with an H3 launch vehicle for three years of observations at a Sun-Earth Lagrangian point (L2). The concept design has been studied by researchers from Japan, U.S., Canada and Europe during the ISAS Phase-A1. Large scale measurements of the CMB B-mode polarization are known as the best probe to detect primordial gravitational waves. The goal of LiteBIRD is to measure the tensor-to-scalar ratio (r) with precision of r < 0:001. A 3-year full sky survey will be carried out with a low frequency (34 - 161 GHz) telescope (LFT) and a high frequency (89 - 448 GHz) telescope (HFT), which achieve a sensitivity of 2.5 \u3bcK-arcmin with an angular resolution 30 arcminutes around 100 GHz. The concept design of LiteBIRD system, payload module (PLM), cryo-structure, LFT and verification plan is described in this paper

Concept design of low frequency telescope for CMB B-mode polarization satellite LiteBIRD

LiteBIRD has been selected as JAXA’s strategic large mission in the 2020s, to observe the cosmic microwave background (CMB) B-mode polarization over the full sky at large angular scales. The challenges of LiteBIRD are the wide field-of-view (FoV) and broadband capabilities of millimeter-wave polarization measurements, which are derived from the system requirements. The possible paths of stray light increase with a wider FoV and the far sidelobe knowledge of -56 dB is a challenging optical requirement. A crossed-Dragone configuration was chosen for the low frequency telescope (LFT : 34–161 GHz), one of LiteBIRD’s onboard telescopes. It has a wide field-of-view (18° x 9°) with an aperture of 400 mm in diameter, corresponding to an angular resolution of about 30 arcminutes around 100 GHz. The focal ratio f/3.0 and the crossing angle of the optical axes of 90◦ are chosen after an extensive study of the stray light. The primary and secondary reflectors have rectangular shapes with serrations to reduce the diffraction pattern from the edges of the mirrors. The reflectors and structure are made of aluminum to proportionally contract from warm down to the operating temperature at 5 K. A 1/4 scaled model of the LFT has been developed to validate the wide field-of-view design and to demonstrate the reduced far sidelobes. A polarization modulation unit (PMU), realized with a half-wave plate (HWP) is placed in front of the aperture stop, the entrance pupil of this system. A large focal plane with approximately 1000 AlMn TES detectors and frequency multiplexing SQUID amplifiers is cooled to 100 mK. The lens and sinuous antennas have broadband capability. Performance specifications of the LFT and an outline of the proposed verification plan are presented

Overview of the medium and high frequency telescopes of the LiteBIRD space mission

LiteBIRD is a JAXA-led Strategic Large-Class mission designed to search for the existence of the primordial gravitational waves produced during the inflationary phase of the Universe, through the measurements of their imprint onto the polarization of the cosmic microwave background (CMB). These measurements, requiring unprecedented sensitivity, will be performed over the full sky, at large angular scales, and over 15 frequency bands from 34 GHz to 448 GHz. The LiteBIRD instruments consist of three telescopes, namely the Low-, Medium-and High-Frequency Telescope (respectively LFT, MFT and HFT). We present in this paper an overview of the design of the Medium-Frequency Telescope (89{224 GHz) and the High-Frequency Telescope (166{448 GHz), the so-called MHFT, under European responsibility, which are two cryogenic refractive telescopes cooled down to 5 K. They include a continuous rotating half-wave plate as the first optical element, two high-density polyethylene (HDPE) lenses and more than three thousand transition-edge sensor (TES) detectors cooled to 100 mK. We provide an overview of the concept design and the remaining specific challenges that we have to face in order to achieve the scientific goals of LiteBIRD