Revisão sistemática na socialização do género na fase de educação da primeira infância

Throughout the early childhood education stage, gender identity is established through the process of socialisation through which boys and girls acquire and construct stereotypes and stereotyped gender roles, in many cases dichotomised and sexist. The general objective guiding this study is to find out about gender socialisation and the acquisition and construction of stereotyped roles and gender stereotypes in boys and girls at the Early Childhood Education stage based on systematic review in the Web of Science, Scopus and Dialnet databases. After a systematic search, 124 documents from all databases made up the final sample. To begin with, a qualitative-quantitative analysis of the selected publications in each of the databases was carried out, followed by a content analysis of the presence of gender roles and/or stereotypes of boys and girls in these studies. We conclude with the identification of a greater number of publications in the Web of Science database on this subject; the existence of a greater number of publications that focus their studies on boys and girls in the second cycle of Early Childhood Education; and the presence of stereotyped roles and gender stereotypes concerning play and toys.A lo largo de la etapa de Educación infantil se configura la identidad de género mediante el proceso de socialización por el cual, niños y niñas adquieren y construyen es­tereotipos y roles estereotipados de género, en muchos casos dicotomizados y sexistas. El objetivo general que guía este estudio es conocer la socialización de género y la adquisición y construcción de roles estereotipados y estereotipos de género en niños y niñas en la etapa de Educación Infantil a partir de la revisión sistemática en las bases de datos Web of Science, Scopus y Dialnet. Tras una búsqueda sistemática, 124 documentos de todas las bases de datos han conformado la muestra final. Para empezar, se ha realizado un análisis cualitativo-cuanti­tativo de las publicaciones seleccionadas en cada una de las bases de datos para en segundo lugar, realizar un análisis de contenido sobre la presencia de roles y/o estereotipos de género en los niños y niñas en dichos estudios. Se concluye con la identificación de un mayor número de publicaciones en la base de datos Web of Science sobre dicha temática; la existencia de un mayor número de publicaciones que centran sus estudios en niños y niñas del Segundo ciclo de Educación Infantil; y la presencia de roles estereotipados y estereotipos de género en relación al juego y los juguetes.Ao longo da fase da educação infantil, a identidade de género é moldada pelo processo de socialização através do qual rapazes e raparigas adquirem e constroem estereótipos e papéis de género estereotipados, em muitos casos dicotomizados e sexistas. O objetivo geral que norteia este estudo é conhecer a socialização de gênero e a aquisição e construção de papéis estereotipados e estereótipos de gênero em meninos e meninas na etapa da Educação Infantil a partir da análise bibliométrica nas bases de dados Web of Science, Scopus e Dialnet. Após uma pesquisa sistemática, 124 documentos de todas as bases de dados formaram a amostra final. Para começar, foi realizada uma análise qualitativa-quantitativa das publicações seleccionadas em cada uma das bases de dados, seguida de uma análise de conteúdo da presença de papéis e/ou estereótipos de género em rapazes e raparigas nestes estudos. Concluímos com a identificação de um maior número de publicações na base de dados Web of Science sobre este assunto; a existência de um maior número de publicações que focalizam os seus estudos em rapazes e raparigas no segundo ciclo de Educação Infantil; e a presença de papéis estereotipados e estereótipos de género em relação a brincadeiras e brinquedos

Activity-Dependent Downscaling of Subthreshold Synaptic Inputs during Slow-Wave-Sleep-like Activity In Vivo.

Activity-dependent synaptic plasticity is critical for cortical circuit refinement. The synaptic homeostasis hypothesis suggests that synaptic connections are strengthened during wake and downscaled during sleep; however, it is not obvious how the same plasticity rules could explain both outcomes. Using whole-cell recordings and optogenetic stimulation of presynaptic input in urethane-anesthetized mice, which exhibit slow-wave-sleep (SWS)-like activity, we show that synaptic plasticity rules are gated by cortical dynamics in vivo. While Down states support conventional spike timing-dependent plasticity, Up states are biased toward depression such that presynaptic stimulation alone leads to synaptic depression, while connections contributing to postsynaptic spiking are protected against this synaptic weakening. We find that this novel activity-dependent and input-specific downscaling mechanism has two important computational advantages: (1) improved signal-to-noise ratio, and (2) preservation of previously stored information. Thus, these synaptic plasticity rules provide an attractive mechanism for SWS-related synaptic downscaling and circuit refinement

Distinct mechanisms of spike timing-dependent LTD at vertical and horizontal inputs onto L2/3 pyramidal neurons in mouse barrel cortex

Spike timing‐dependent plasticity (STDP ) is an attractive candidate to mediate the synaptic changes that support circuit plasticity in sensory cortices during development. STDP is prevalent at excitatory synapses, but it is not known whether the underlying mechanisms are universal, or whether distinct mechanisms underpin STDP at different synapses. Here, we set out to compare and contrast STDP at vertical layer 4 and horizontal layer 2/3 inputs onto postsynaptic layer 2/3 neurons in the mouse barrel cortex. We find that both vertical and horizontal inputs show STDP , but that they display different time windows for induction of timing‐dependent long‐term depression (t‐LTD ). Moreover, whereas t‐LTD at vertical inputs requires presynaptic NMDA receptors and is expressed presynaptically, using paired recordings we find that t‐LTD at horizontal inputs requires postsynaptic NMDA receptors and is expressed postsynaptically. These results demonstrate that similar forms of plasticity on the same postsynaptic neuron can be mediated by distinct mechanisms, and suggest that these forms of plasticity may enable these two types of cortical synapses to support different functions

TRACTOR_DB: a database of regulatory networks in gamma-proteobacterial genomes

Experimental data on the Escherichia coli transcriptional regulatory system has been used in the past years to predict new regulatory elements (promoters, transcription factors (TFs), TFs' binding sites and operons) within its genome. As more genomes of gamma-proteobacteria are being sequenced, the prediction of these elements in a growing number of organisms has become more feasible, as a step towards the study of how different bacteria respond to environmental changes at the level of transcriptional regulation. In this work, we present TRACTOR_DB (TRAnscription FaCTORs' predicted binding sites in prokaryotic genomes), a relational database that contains computational predictions of new members of 74 regulons in 17 gamma-proteobacterial genomes. For these predictions we used a comparative genomics approach regarding which several proof-of-principle articles for large regulons have been published. TRACTOR_DB may be currently accessed at http://www.bioinfo.cu/Tractor_DB, http://www.tractor.lncc.br/ or at http://www.cifn.unam.mx/Computational_Genomics/tractorDB. Contact Email id is [email protected]

Presynaptic self-depression at developing neocortical synapses

A central tenet of most theories of synaptic modification during cortical development is that correlated activity drives plasticity in synaptically connected neurons. Unexpectedly, however, using sensory-evoked activity patterns recorded from the developing mouse cortex in vivo, the synaptic learning rule that we uncover here relies solely on the presynaptic neuron. A burst of three presynaptic spikes followed, within a restricted time window, by a single presynaptic spike induces robust long-term depression (LTD) at developing layer 4 to layer 2/3 synapses. This presynaptic spike pattern-dependent LTD (p-LTD) can be induced by individual presynaptic layer 4 cells, requires presynaptic NMDA receptors and calcineurin, and is expressed presynaptically. However, in contrast to spike timing-dependent LTD, p-LTD is independent of postsynaptic and astroglial signaling. This spike pattern-dependent learning rule complements timing-based rules and is likely to play a role in the pruning of synaptic input during cortical development

Inorganic carbon stimulates the metabolic routes related to the polyhydroxybutyrate production in a Synechocystis sp. strain (cyanobacteria) isolated from wastewater

Cyanobacteria are capable of transforming CO2 into polyhydroxybutyrate (PHB). In this study, different inorganic carbon concentrations (0–2 gC L−1) were evaluated for a Synechocystis sp. strain isolated from wastewater. Quantitative RT-qPCR was also performed to decipher the links between inorganic carbon and PHB and glycogen metabolism. 2 gC L−1 of bicarbonate stimulated cell growth, nutrients consumption and production of PHB. Using this concentration, a 14%dcw of PHB and an average productivity of 2.45 mgPHB L−1 d−1 were obtained. Gene expression analysis revelated that these conditions caused the overexpression of genes related to glycogen and PHB synthesis. Moreover, a positive correlation between the genes codifying for the glycogen phosphorylase, the acetyl-CoA reductase and the poly(3-hydroxyalkanoate) polymerase was found, meaning that PHB synthesis and glycogen catabolism are strongly related. These results provide an exhaustive evaluation of the effect of carbon on the PHB production and cyanobacterial metabolism

Evaluating the functional, sexual and seasonal variation in the chemical constituents from feces of adult Iberian wolves (Canis lupus signatus)

Chemical signals deposited in feces play an important role in intraspecific and interspecific communication of many mammals. We collected fresh feces of adult wolves from wild breeding groups. All samples visually identified as belonging to wolves were subsequently identified to species level by sequencing a small fragment of mtDNA and sexed typing DBX6 and DBY7 sex markers. Using gas chromatography-mass spectrometry (GC–MS), we identified 56 lipophilic compounds in the feces, mainly heterocyclic aromatic organic compounds, such as indole or phenol, but also steroids, such as cholesterol, carboxylic acids and their esters between n-C4 and n-C18, aldehydes, alcohols and significant quantities of squalene and α-tocopherol, which would increase the chemical stability of feces on humid substrates. There was variability in the number and proportions of compounds between sexes, which could be indicative of their function as chemical signals. We also found variability in different reproductive states, especially in odorous compounds, steroids and α-tocopherol. Feces with a presumed marking function had higher proportions of α-tocopherol and steroids than feces with non-marking function. These compounds could be involved in intragroup and intergroup communication of wolves and their levels in feces could be directly related with the wolf’s sex and physiological and reproductive status.Financial support was provided by the project MCI-CGL2008-02119/BOS

Inorganic carbon stimulates the metabolic routes related to the polyhdroxybutyrate production in a Synechocystis sp. strain (cyanobacteria) isolated from wastewater

Cyanobacteria are capable of transforming CO2 into polyhydroxybutyrate (PHB). In this study, different inorganic carbon concentrations (0–2 gC L-1) were evaluated for a Synechocystis sp. strain isolated from wastewater. Quantitative RT-qPCR was also performed to decipher the links between inorganic carbon and PHB and glycogen metabolism. 2 gC L-1 of bicarbonate stimulated cell growth, nutrients consumption and production of PHB. Using this concentration, a 14%dcw of PHB and an average productivity of 2.45 mgPHB L-1 d-1 were obtained. Gene expression analysis revelated that these conditions caused the overexpression of genes related to glycogen and PHB synthesis. Moreover, a positive correlation between the genes codifying for the glycogen phosphorylase, the acetyl-CoA reductase and the poly(3-hydroxyalkanoate) polymerase was found, meaning that PHB synthesis and glycogen catabolism are strongly related. These results provide an exhaustive evaluation of the effect of carbon on the PHB production and cyanobacterial metabolism.Authors want to acknowledge the support received by the Spanish Ministry of Science, Innovation and Universities (MCIU), the Research National Agency (AEI), and the European Regional Development Fund (FEDER) [AL4BIO, RTI2018-099495-B-C21]. Estel Rueda thanks the Spanish Ministry of Education, Culture and Sport [FPU18/04941] for her grant. Joaquim Vila is a Serra Húnter Fellow (Generalitat de Catalunya). Rubén Díez-Montero would also like to thank the Spanish Ministry of Industry and Economy for his research grants [IJC2019-042069-I].Peer ReviewedPostprint (published version

Evaluación cualitativa del sistema de recogida de sangre en Cataluña

La donación de sangre es indispensable para el mantenimiento de una prestación sanitaria de calidad. El estancamiento del número de donaciones en Cataluña hizo pertinente poner en marcha una evaluación externa del sistema de recogida de sangre que pudiera orientar las políticas futuras. Se diseñó una evaluación del sistema de hemocaptación en Cataluña adoptando una metodología cualitativa, que incluyó el uso de una variedad de técnicas como la observación participante, las entrevistas individuales, las entrevistas grupales y las técnicas documentales. El trabajo de campo se llevó a cabo en la totalidad de los sectores hemopáticos de Cataluña del 23 de noviembre al 4 de diciembre de 1998. El análisis de la información siguió parcialmente las prescripciones de la Grounded Theory y de algunas modalidades de análisis de contenido temático usando la herramienta informática para el análisis cualitativo de datos textuales Atlas/ti. Los resultados muestran las características básicas y las carencias del proceso de hemocaptación, describen los puntos fuertes y débiles del proceso de hemocaptación y de su organización, detallan los argumentos sobre la donación de sangre que utilizan los agentes implicados en el proceso, y dan cuenta de las características atribuidas a los donantes.Blood donation is indispensable for quality health service provision. The stagnation of the number of donations in Catalonia prompted up an external evaluation of the system of blood collection that could orient the future policies. An evaluation of blood-donor services in Catalonia was designed adopting a qualitative methodology. The evaluation design included the use of a variety of techniques namely participant observation, individual interviews, group interviews and documentary techniques. The field work was carried out in all blood-donation sectors of Catalonia from the 23th November to the 4th December, 1998. Using Atlas/ti, a computer science tool for the qualitative analysis of textual data, the information analysis partially followed the prescriptions of "grounded theory" and of some modalities of thematic content analysis. The results show the basic characteristics and deficiencies of the process and its organization, describing their strong and weak points. The findings also detail arguments on blood donation that the agents implied in the process, donors an professionals, use. We also report what reasons the blood-donor service managers/providers gave for donating blood, and what perceptions they had of blood donors

Globalización y dinamismo manufacturero. México y otros países emergentes.

Análisis de algunos de los problemas por los que atraviesa la industria manufacturera en México como consecuencia de la aplicación de las políticas neoliberales en los últimos 30 años. A saber, apertura comercial indiscriminada; incremento de los flujos de inversión extranjera directa, y la no intervención del Estado. En la primera parte se analizan los factores principales que han impedido o dificultado a la manufactura impulsar el crecimiento y el desarrollo económicos. En la segunda parte se estudian las experiencias del proceso de industrialización en tres países emergentes (Corea del Sur, China y Brasil). En estos países, ha sido la participación decidida y directa del Estado, lo que ha permitido un desarrollo manufacturero exitoso