Interspecific and intraspecific gene variability in a 1-Mb region containing the highest density of NBS-LRR genes found in the melon genome

Background: Plant NBS-LRR -resistance genes tend to be found in clusters, which have been shown to be hot spots of genome variability. In melon, half of the 81 predicted NBS-LRR genes group in nine clusters, and a 1 Mb region on linkage group V contains the highest density of R-genes and presence/absence gene polymorphisms found in the melon genome. This region is known to contain the locus of Vat, an agronomically important gene that confers resistance to aphids. However, the presence of duplications makes the sequencing and annotation of R-gene clusters difficult, usually resulting in multi-gapped sequences with higher than average errors. - Results: A 1-Mb sequence that contains the largest NBS-LRR gene cluster found in melon was improved using a strategy that combines Illumina paired-end mapping and PCR-based gap closing. Unknown sequence was decreased by 70% while about 3,000 SNPs and small indels were corrected. As a result, the annotations of 18 of a total of 23 NBS-LRR genes found in this region were modified, including additional coding sequences, amino acid changes, correction of splicing boundaries, or fussion of ORFs in common transcription units. A phylogeny analysis of the R-genes and their comparison with syntenic sequences in other cucurbits point to a pattern of local gene amplifications since the diversification of cucurbits from other families, and through speciation within the family. A candidate Vat gene is proposed based on the sequence similarity between a reported Vat gene from a Korean melon cultivar and a sequence fragment previously absent in the unrefined sequence. - Conclusions: A sequence refinement strategy allowed substantial improvement of a 1 Mb fragment of the melon genome and the re-annotation of the largest cluster of NBS-LRR gene homologues found in melon. Analysis of the cluster revealed that resistance genes have been produced by sequence duplication in adjacent genome locations since the divergence of cucurbits from other close families, and through the process of speciation within the family a candidate Vat gene was also identified using sequence previously unavailable, which demonstrates the advantages of genome assembly refinements when analyzing complex regions such as those containing clusters of highly similar genes

Historias contadas por profesores de cursos a distancia: experiencias de aprendizaje mediadas por la tecnología ¿Centralidad del estudiante o disminución del rol de facilitación?

En la presente investigación, se analizan 15 textos de experiencias de profesores al impartir cursos en línea, se adoptó el método de narrativa. Se destacan en el estudio el rol del facilitador y la centralidad del estudiante. Desde las historias, se advierte una naciente disociación entre lo que es asumido hacia tales temas por profesores y aprendices, toda vez que el rol fuerte de facilitación auto percibido por los profesores es apenas reconocido por quienes aprenden, los profesores a su vez, solo reconocen una centralidad parcial de los estudiantes al tiempo que éstos empiezan a hacer cuestionamientos abiertos al rol del facilitador.In this work, 15 narratives of online course experiences, from the teachers, were analyzed. In the study, two main themes are emerging through the qualitative data analysis: the tutor role and the student autonomy. From the narratives analyzed, is beginning a special dissociation to this topics by teachers and students, while the teachers think about their strong tutor role when they are tutoring online courses, the students think different abut the same role, instead it, they are questioning the performance of the online tutor

Does the midbrain dorsolateral periaqueductal grey have direct connections with the pontine A5 region? A neuropharmacologic and electrophysiological study

This study has been performed in spontaneously breathing anesthetised rats. We have analysed the possible interactions between the midbrain dorsolateral periaqueductal grey matter (dlPAG) and the pontine A5 region. Electrical stimulations of the dlPAG (1 ms pulses, 20-30 μA given at 100 Hz for 5s) were elicited and the evoked cardiorespiratory changes were analysed before and after ipsilateral blockade of the neurotransmission within the A5 region by means of microinjections of muscimol (50 nl, 0.25 nmol, 5s). Electrical stimulations evoked the classical “defence response” characterized by tachipnoea, hypertension and tachycardia. Tachipnoea consisted of an inspiratory facilitatory response [increase in respiratory rate (p<0.001) due to a decrease in expiratory time (p<0.01)] and was accompanied by a pressor (p<0.001) and tachycardic (p<0.001) response. Muscimol microinjected within the A5 region reduced pressor (p<0.05), tachycardic (p<0.001) and tachypnoeic (p<0.001) responses evoked to dlPAG electrical stimulations. Finally, in order to assess functional interactions between A5 and dlPAG, extracellular recordings of 40 putative A5 cells during dlPAG electrical stimulation were recorded. 16 cells were affected by dlPAG stimulation (40%). 3 cells showed orthodromic activation (14.2 ± 1.7 ms). 5 cells were excited (10.1 ± 1.6 ms). 7 cells decreased spontaneous activity to dlPAG stimulation. 24 cells were not modified by dlPAG stimulation (2 presented a respiratory pattern and 1 presented a cardiovascular pattern). These results contribute with new data on the role of the A5 region neurones in the modulation of the cardiorespiratory response evoked on dlPAG stimulation.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Constructive heuristics for the unrelated parallel machines scheduling problem with machine eligibility and setup times

This work considers a scheduling problem identified in a factory producing customised Heating, Ventilation and Air Conditioning (HVAC) equipment. More specifically, the metal folding section is modelled as unrelated parallel machines with machine eligibility and sequence-dependent setup times. The objective under consideration is the minimisation of the total tardiness. The problem is known to be NP-hard so approximate methods are needed to solve real-size instances. In order to embed the scheduling procedure into a decision support system providing high-quality solutions in nearly real time, the goal of this paper is to develop fast, efficient constructive heuristics for the problem. Due to the lack of methods for this specific problem, some existing heuristics and one metaheuristic are selected from related problems and adapted. In addition, a set of heuristics with novel repair and improvement phases are proposed. The performance of the methods adapted and the proposals are compared with the optimal/approximate solutions obtained by a solver for an MILP in two sets of instances with small and medium sizes. Additionally, big-size instances (representing more realistic cases for our company) have been solved using the proposed constructive heuristics, providing efficient solutions in negligible computational times. Even if the adaptation of heuristics performs reasonably well, these are outperformed by the new heuristic proposed in this paper. In addition, when the new heuristic is embedded in the metaheuristic adapted from a related the problem, the results obtained are excellent in terms of the quality of the solution, even if the computational effort is somewhat higher.Ministerio de Ciencia en Innovación. “PROMISE

Monitoring System of Environmental Variables Using a Wireless Sensor Network and Platforms of Internet of Things

This work proposes a system for collecting meteorological data using a Wireless Sensor Network (WSN), that is able to transmit data in real-time. The system automatizes the process of collecting the data in a continuous manner for long periods of time, for this, the module is equipped with a source of solar energy that allows autonomous operation. In order to obtain viability of design and prototype implementation, the construction of two systems was proposed based on DigiMesh and Wi-Fi; those prototypes could be applied to different scenarios such as urban and rural areas. Additionally, it was performed an evaluation of broadcasting of information to platforms of Internet of Things (IoT), where the data collected by the nodes will be managed and displayed. This system was conceived as a low-cost alternative compared with conventional weather stations that offer these facilities and are based on free hardware and software components. Finally, the validation of the obtained results was performed using a statistical analysis with the collected data of the weather station Davis Vantage Pro, obtaining a maximum average relative error of 4.93%

Data on recovery of 21 amino acids, 9 biogenic amines and ammonium ions after spiking four different beers with five concentrations of these analytes

A novel chromatographic method for the simultaneous analysis of nine biogenic amines, 21 amino acids and ammonium ions in beer has been recently described in “A UHPLC method for the simultaneous analysis of biogenic amines, amino acids and ammonium ions in beer” (Redruello et al., 2017) [1]. The present article provides recovery data of the 31 analytes after spiking four different beers with five concentrations of each analyte (15, 30, 60, 120 and 240 µM).This work was performed with the financial support of the Spanish Ministry of Economy and Competitiveness (AGL2013-45431-R) and the Plan for Science, Technology and Innovation 2013–2017 of the Principality of Asturias, which is co-funded by the European Regional Development Fund (GRUPIN14-137).Peer reviewe

High presence/absence gene variability in defense-related gene clusters of Cucumis melo

Changes in the copy number of DNA sequences are one of the main mechanisms generating genome variability in eukaryotes. These changes are often related to phenotypic effects such as genetic disorders or novel pathogen resistance. The increasing availability of genome sequences through the application of next-generation massive sequencing technologies has allowed the study of genomic polymorphisms at both the interspecific and intraspecific levels, thus helping to understand how species adapt to changing environments through genome variability. Data on gene presence/absence variation (PAV) in melon was obtained by resequencing a cultivated accession and an old-relative melon variety, and using previously obtained resequencing data from three other melon cultivars, among them DHL92, on which the current draft melon genome sequence is based. A total of 1,697 PAV events were detected, involving 4.4% of the predicted melon gene complement. In all, an average 1.5% of genes were absent from each analyzed cultivar as compared to the DHL92 reference genome. The most populated functional category among the 304 PAV genes of known function was that of stress response proteins (30% of all classified PAVs). Our results suggest that genes from multi-copy families are five times more likely to be affected by PAV than singleton genes. Also, the chance of genes present in the genome in tandem arrays being affected by PAV is double that of isolated genes, with PAV genes tending to be in longer clusters. The highest concentration of PAV events detected in the melon genome was found in a 1.1 Mb region of linkage group V, which also shows the highest density of melon stress-response genes. In particular, this region contains the longest continuous gene-containing PAV sequence so far identified in melon. The first genome-wide report of PAV variation among several melon cultivars is presented here. Multi-copy and clustered genes, especially those with putative stress-response functions, were found to be particularly affected by PAV polymorphisms. As cucurbits are known to possess a significantly lower number of defense-related genes compared to other plant species, PAV variation may play an important role in generating new pathogen resistances at the subspecies level. In addition, these results show the limitations of single reference genome sequences as the only basis for characterization and cloning of resistance genes

A smart power electronic multiconverter for the residential sector

El futuro de la red incluye la generación distribuida y las tecnologías de red inteligente. Los sistemas de gestión del lado de la demanda (DSM) también serán esenciales para lograr un alto nivel de confiabilidad y robustez en los sistemas de energía. Para hacer eso, es necesario expandir la Infraestructura de medición avanzada (AMI) y los Sistemas de gestión de energía (EMS). La dirección de la tendencia es hacia la creación de centros de recursos energéticos, como el concepto de comunidad inteligente. Este documento presenta un sistema multiconvertidor inteligente para el sector residencial / vivienda con un Sistema de Almacenamiento de Energía Híbrido (HESS) que consta de supercapacitador y batería, y con integración de fuente de energía fotovoltaica (PV) local. El dispositivo funciona como una unidad de energía distribuida ubicada en cada casa de la comunidad, recibiendo puntos de ajuste de energía activos proporcionados por una comunidad inteligente EMS. Este SGA central es responsable de administrar los flujos de energía activa entre la red eléctrica, las fuentes de energía renovables, los equipos de almacenamiento y las cargas existentes en la comunidad. El multiconvertidor propuesto es responsable de cumplir con los puntos de referencia de potencia activa de referencia con la calidad de potencia adecuada; garantizando que los módulos fotovoltaicos locales funcionen con un algoritmo de seguimiento del punto de máxima potencia (MPPT); y prolongando la vida útil de la batería gracias a un funcionamiento cooperativo del HESS. Se ha desarrollado un modelo de simulación para mostrar el funcionamiento detallado del sistema. Finalmente, se implementó un prototipo de la plataforma de multiconversores y se realizaron algunas pruebas experimentales para validarlo.The future of the grid includes distributed generation and smart grid technologies. Demand Side Management (DSM) systems will also be essential to achieve a high level of reliability and robustness in power systems. To do that, expanding the Advanced Metering Infrastructure (AMI) and Energy Management Systems (EMS) are necessary. The trend direction is towards the creation of energy resource hubs, such as the smart community concept. This paper presents a smart multiconverter system for residential/housing sector with a Hybrid Energy Storage System (HESS) consisting of supercapacitor and battery, and with local photovoltaic (PV) energy source integration. The device works as a distributed energy unit located in each house of the community, receiving active power set-points provided by a smart community EMS. This central EMS is responsible for managing the active energy flows between the electricity grid, renewable energy sources, storage equipment and loads existing in the community. The proposed multiconverter is responsible for complying with the reference active power set-points with proper power quality; guaranteeing that the local PV modules operate with a Maximum Power Point Tracking (MPPT) algorithm; and extending the lifetime of the battery thanks to a cooperative operation of the HESS. A simulation model has been developed in order to show the detailed operation of the system. Finally, a prototype of the multiconverter platform has been implemented and some experimental tests have been carried out to validate it.Ministerio de Economía y Competitividad (España) y Fondos FEDER: Proyecto TEC2013-47316-C3-3-PpeerReviewe

Laryngeal effects of stimulation of the dorsomedial hypothalamic nucleus and perifornical area in spontaneously breathing anaesthetized rats

Background: Stimulation of the dorsomedial hypothalamic nucleus and perifornical area (DMH-PeF) in rats evokes a cardiorespiratory response characterised by an increase in blood pressure, heart rate and respiratory frequency. In previous studies (Díaz-Casares et al., 2012; López-González et al., 2018) we have shown a functional interaction between DMH-PeF and pontine structures (A5 region and Parabrachial Complex). We have also shown that rostral and ventral pontine structures are involved in the changes of laryngeal caliber (Lara et al., 2002). The aim of this study was to characterize the relations between hypothalamic and pontine neuronal circuits involved in laryngeal activity and its effect on vocalization. Methods: Experimental studies were carried out with non-inbred male rats (n=7). Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2 mg/ kg, i.v., as necessary). A double tracheal cannulation (upwards in direction of the glottis for the “glottis isolated in situ” technique) was done. Subglottic pressure was recorded with an aneroid transducer by passing a stream of humidified medical air upwards through the larynx at a constant rate of 30-70 ml/min with a thermal mass digital air flow meter controller. Electrical stimulation of the DMH-PeF using concentric bipolar electrodes (1 ms pulses, 20-40 µA, 100 Hz for 5 s) was performed. Respiratory flow, pleural pressure, blood pressure, heart rate and ECG activity were also recorded. Results: DMH-PeF stimulation evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,001) accompanied with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,001), together with a pressor (p<0,001) and a tachycardic response (p<0,001). Conclusions: The results of our study contribute with new data on the role of the DMH-PeF in the mechanisms controlling subglottic pressure and laryngeal activity.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech