Advanced Tuneable Micronanoplatforms for Sensitive and Selective Multiplexed Spectroscopic Sensing via Electro-Hydrodynamic Surface Molecular Lithography

Micro- and nanopatterning of materials, one of the cornerstones of emerging technologies, has transformed research capabilities in lab-on-a-chip diagnostics. Herein, a micro- and nanolithographic method is developed, enabling structuring materials at the submicron scale, which can, in turn, accelerate the development of miniaturized platform technologies and biomedical sensors. Underpinning it is the advanced electro-hydrodynamic surface molecular lithography, via inducing interfacial instabilities produces micro- and nanostructured substrates, uniquely integrated with synthetic surface recognition. This approach enables the manufacture of design patterns with tuneable feature sizes, which are functionalized via synthetic nanochemistry for highly sensitive, selective, rapid molecular sensing. The development of a high-precision piezoelectric lithographic rig enables reproducible substrate fabrication with optimum signal enhancement optimized for functionalization with capture molecules on each micro- and nanostructured array. This facilitates spatial separation, which during the spectroscopic sensing, enables multiplexed measurement of target molecules, establishing the detection at minute concentrations. Subsequently, this nano-plasmonic lab-on-a-chip combined with the unconventional computational classification algorithm and surface enhanced Raman spectroscopy, aimed to address the challenges associated with timely point-of-care detection of disease-indicative biomarkers, is utilized in validation assay for multiplex detection of traumatic brain injury indicative glycan biomarkers, demonstrating straightforward and cost-effective micro- and nanoplatforms for accurate detection.</p

PENGARUH PERUBAHAN SUHU PADA PROPERTI ADSORPSI DAN DESORPSI THERMOSENSITIVE NIPAM-co-DMAAPS GEL

Gel adsorben yang memiliki kemampuan mengadsorpsi dan mendesorpsi ion secara simultan dengan perubahan suhu (temperature swing) dihasilkan melalui reaksi kopolimerisasi radikal bebas antara N-isopropylacrylamide (NIPAM), dan N,Ndimethyl( acrylamidopropyl)ammonium propane sulfonate (DMAAPS). NIPAM dipergunakan sebagai agen thermosensitive dan DMAAPS digunakan sebagai agen pengadsorpsi. Tujuan dari penelitian ini adalah untuk mempelajari pengaruh perubahan suhu terhadap properti swelling, adsorpsi, dan desorpsi ion pada thermosensitive NIPAM-co-DMAAPS gel dan hubungan antara properti-properti tersebut. NaNO3 dipergunakan sebagai larutan uji dalam mempelajari properti swelling, adsorpsi, dan desorpsi. Uji swelling dilakukan dengan mengukur diameter dari silinder gel pada keadaan kering dan setelah kontak dengan larutan NaNO3 selama 12 jam. Untuk uji adsorpsi dilakukan dengan memasukkan satu gram crush gel ke dalam 20 ml larutan NaNO3 pada berbagai konsentrasi dan suhu selama 12 jam yang disertai dengan pengadukan. Sedangkan uji desorpsi dilakukan dengan memasukkan gel yang diperoleh pada uji adsorpsi yang telah dikeringkan sebelumnya ke dalam distilled water selama 12 jam 1. Jovanio Bosco C. G. A. 2311 100 085 2. Desi Ratnasari 2311 100 105 1. Dr. Eva Oktavia Ningrum, ST., MS. 2. Prida Novarita Trisanti, ST., M.T. pada suhu yang ditetapkan disertai dengan pengadukan. Gel hasil adsorpsi dan desorpsi dipisahkan dari larutannya untuk mendapatkan konsentrasi larutan akhir, kemudian larutan ini dianalisa menggunakan Atomic Absorption Spectrophotometry (AAS) Dari hasil penelitian diketahui bahwa pada suhu tinggi, adsorpsi ion pada kopolimer gel nilainya rendah namun prosentase desorpsi dan nilai swelling degree cenderung tinggi. Nilai dari swelling degree, adsopsi, dan desorpsi ion pada kopolimer gel juga berbanding lurus terhadap konsentrasi larutan NaNO3 yang digunakan. Berdasarkan hubungan antara properti swelling dan adsorpsi dapat disimpulkan bahwa pada nilai swelling degree yang rendah, adsorpsi ion pada kopolimer gel menunjukkan nilai yang konstan. Namun dengan meningkatnya nilai swelling degree, adsorpsi ion cenderung menurun. ====================================================================================== Adsorbent gels with ability to adsorb and desorb ion simultaneously with temperature swing are synthesized by free radical copolymerization reaction of N-isopropylacrylamide (NIPAM) and N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate (DMAAPS). In this study, NIPAM acts as a thermosensitive agent and DMAAPS as an adsorbent agent. The purposes of this research are to investigate the effects of temperature on the swelling, adsorption and desorption behaviors of ion onto thermosensitive NIPAM-co-DMAAPS gel, and to study the relationship between these behaviors. NaNO3 solution was selected as the target solution and used in swelling, adsorption and desorption test. Swelling test was done by measuring diameter of the cylindrical gel after and before immersion in the NaNO3 solution for 12 hours. Adsorption test was carried out by introducing one gram grinded gels into 20 ml NaNO3 solution of desired concentration and temperature under stirring for 12 hours. Conversely, desorption test was performed by introducing the gels which were obtained from adsorption experiment and dried previously, into distilled water at the desired temperature and stirred gently for 12 hours. After adsorption and desorption, the gels were removed from the solution by centrifuge in order to measure the residual ion concentration in the solution. 1. Jovanio Bosco C. G. Amaral 2311 100 085 2. Desi Ratnasari 2311 100 105 1. Dr. Eva Oktavia Ningrum, ST., MS. 2. Prida Novarita Trisanti, ST., M.T. The concentrations of the solutions which were obtained from adsorption and desorption experiment were then analyzed by atomic Absorption Spectrophotometry (AAS). The swelling degree of the gels as measured by using millimeter block. The result revealed that at high temperature, the amount of ion adsorbed by the gels was low. On the contrary, the gel exhibited a high degree of swelling and high amount of ion desorbed by the gel. Moreover, higher concentration of NaNO3 resulting in the higher value of the swelling degree, adsorption and desorption ability. Based on the relationship between the swelling properties and adsorption properties, it can be concluded that at a lower degree of swelling, the amount of ion adsorbed onto copolymer gel showed a constant value. However, the amount of ion adsorbed decrease with further increasing the swelling degree

Noncommutative massive Thirring model in three-dimensional spacetime

We evaluate the noncommutative Chern-Simons action induced by fermions interacting with an Abelian gauge field in a noncommutative massive Thirring model in (2+1)-dimensional spacetime. This calculation is performed in the Dirac and Majorana representations. We observe that in Majorana representation when $\theta$ goes to zero we do not have induced Chern-Simons term in the dimensional regularization scheme.Comment: Accepted to Phys. Rev. D; 9 pages, Revtex4, no figures, references added, minor improvements, Eq.31 correcte

Correlation and disorder-enhanced nematic spin response in superconductors with weakly broken rotational symmetry

Recent experimental and theoretical studies have highlighted the possible role of a electronic nematic liquid in underdoped cuprate superconductors. We calculate, within a model of d-wave superconductor with Hubbard correlations, the spin susceptibility in the case of a small explicitly broken rotational symmetry of the underlying lattice. We then exhibit how the induced spin response asymmetry is strongly enhanced by correlations as one approaches the instability to stripe order. In the disorder-induced stripe phase, impurities become spin nematogens with a C_2 symmetric impurity resonance state, and the disorder-averaged spin susceptibility remains only C_2 symmetric at low energies, similar to recent data from neutron scattering experiments on underdoped YBCO.Comment: 6 pages, 5 figure

A Consistent Noncommutative Field Theory: the Wess-Zumino Model

We show that the noncommutative Wess-Zumino model is renormalizable to all orders of perturbation theory. The noncommutative scalar potential by itself is non-renormalizable but the Yukawa terms demanded by supersymmetry improve the situation turning the theory into a renormalizable one. As in the commutative case, there are neither quadratic nor linear divergences. Hence, the IR/UV mixing does not give rise to quadratic infrared poles.Comment: 22 pages, 7 figures. Added references. Typos correcte

UniPlonk: Plonk with Universal Verifier

We propose UniPlonK, a modification of the PlonK protocol that uniformizes the Verifier’s work for families of circuits. Specifically, a single fixed-cost “Universal Verifier” can check proofs for circuits of different: sizes, public input lengths, selector polynomials, copy constraints, and even different custom gate sets. UniPlonK therefore extends the universality of PlonK beyond the SRS; it enables a single “Universal Verifier Circuit” capable of verifying proofs from different PlonK circuits. The Universal Verifier’s marginal cost over the ordinary Plonk verifier is small: for circuits using only the vanilla Plonk gate, the Universal Verifier performs a number of additional field multiplications proportional to the logarithm of the maximum supported circuit size; it incurs no additional elliptic curve operations. For circuits using custom gates, the Universal Verifier incurs additional elliptic curve arithmetic only when verifying proofs from circuits that do not use all supported gate types. For circuits that use all supported gates, the Universal Verifier’s additional cost consists only of field multiplications proportional to the logarithm of the maximum supported circuit size, the number of custom gate types, and the number of witness variables used by these gates. In both settings (vanilla-only and custom gates) the marginal cost to the prover is a fixed-base MSM of size ℓ, the length of the public input vector

New links between SOD1 and metabolic dysfunction from a yeast model of amyotrophic lateral sclerosis

A number of genes have been linked to familial forms of the fatal motor neuron disease amyotrophic lateral sclerosis (ALS). Over 150 mutations within the gene encoding superoxide dismutase 1 (SOD1) have been implicated in ALS, but why such mutations lead to ALS-associated cellular dysfunction is unclear. In this study, we identify how ALS-linked SOD1 mutations lead to changes in the cellular health of the yeast Saccharomyces cerevisiae We find that it is not the accumulation of aggregates but the loss of Sod1 protein stability that drives cellular dysfunction. The toxic effect of Sod1 instability does not correlate with a loss of mitochondrial function or increased production of reactive oxygen species, but instead prevents acidification of the vacuole, perturbs metabolic regulation and promotes senescence. Central to the toxic gain-of-function seen with the SOD1 mutants examined was an inability to regulate amino acid biosynthesis. We also report that leucine supplementation results in an improvement in motor function in a Caenorhabditis elegans model of ALS. Our data suggest that metabolic dysfunction plays an important role in Sod1-mediated toxicity in both the yeast and worm models of ALS

Pengaruh Perubahan Suhu pada Properti Adsorpsi dan Desorpsi Thermosensitive NIPAM-co¬-DMAAPS Gel

Adsorbent gels with ability to absorb and desorb ion simultaneously with temperature swing are synthesized by free radical copolymerization reaction of N-isopropylacrylamide (NIPAM) and N,N-dimethyl(acrylamidopropyl)ammonium propane sulfonate (DMAAPS). In this study, NIPAM acts as a thermosensitive agent and DMAAPS as an adsorbent agent. The purposes of this research are to investigate the effects of temperature on the swelling, adsorption and desorption behaviors of ion onto thermosensitive NIPAM-co-DMAAPS gel, and to study the relationship between these behaviors. NaNO3 solution was selected as the target solution and used in swelling, adsorption and desorption test. Swelling test was done by measuring diameter of the cylindrical gel after and before immersion in the NaNO3 solution for 12 hours. The result revealed that at high temperature, the amount of ion adsorbed onto the gels was low. On the contrary, the gel exhibited a high degree of swelling and high amount of ion desorbed from the gel. Moreover, higher concentration of NaNO3 solution resulting in the higher value of the swelling degree, adsorption and desorption ability. Based on the relationship between the swelling properties and adsorption properties, it can be concluded that at a lower degree of swelling, the amount of ion adsorbed onto the gels showed a constant value. However, the amount of ion adsorbed decrease with further increasing the swelling degree