Electrical-Impedance Plethysmography - its Use in Studying the Cerebral-Circulation of the Rabbit

Electrical impedance plethysmography (EIP) is a noninvasive method that may be useful for both the continuous and serial measurement of changes in pulsatile cerebral blood volume and perhaps cerebral blood flow (CBF). It has not been well validated by comparison with other methods. To attempt to validate the EIP technique, the relationship between the peak amplitude of the transcranial, cardiac-synchronous impedance waveform (dZ(p)) and cerebral blood flow measured by the radiolabelled microsphere technique (CBF(rlm)) and laser Doppler spectroscopy (CBF(lds) was studied in rabbits. CBF was altered by inducing hypertension using metaraminol, hypotension by controlled haemorrhage or hypocarbia by hyperventilation. Twenty-three comparisons between dZ(p) and CBF(lds) and 19 comparisons with CBF(rim) were made in eight rabbits. The percentage change between each measurement using the three techniques in each animal was calculated. Using pooled data from all the animals, the linear regression equations were dZ(p) = 0.5 CBF(rlm) + 33 (r = 0.38, p = 0.22, SE = 79) and dZ(p) = 0-84 CBF(lds) + 19.6 (r = 0.46, p = 0.09, SE = 72). It is concluded that, in the anaesthetised rabbit, when large changes in CBF are induced by the manoeuvres described above, changes in dZ(p) correlate very weakly with changes in either cortical or global CBF, and are influenced by other factors such as pulsatile intracranial blood volume